The Experts below are selected from a list of 321 Experts worldwide ranked by ideXlab platform
Susan Lightman - One of the best experts on this subject based on the ideXlab platform.
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cytokine production and mrna expression by conjunctival t cell lines in chronic allergic eye disease
Clinical & Experimental Allergy, 1999Co-Authors: Vl Calder, Andrea Leonardi, Roger J Buckley, Susan Lightman, Antonio G Secchi, G Jolly, M Hingorani, P AdamsonAbstract:Background Activated CD4(+) T cells, mast cells and eosinophils are the main cytokine-producing cell-types infiltrating the conjunctiva during chronic allergic eye diseases. Interactions between these cells are thought to play an important immunopathogenic role in these disorders (giant papillary conjunctivitis; vernal Keratoconjunctivitis; atopic Keratoconjunctivitis).Objective The objective was to compare the cytokine profiles of conjunctival T-cell lines from patients with different forms of chronic allergic eye disease.Methods T cells were isolated from conjunctival biopsies and non-specifically expanded into lines. The lines were immunophenotyped by flow cytometry. Cytokine production was quantified by immunoassays and more sensitive molecular techniques were used to investigate cytokine mRNA expression to identify the presence of interleukin (IL)-2, IL-4 and interferon (IFN)-gamma transcripts.Results Following four to six rounds of stimulation, the conjunctival T-cell populations were CD3(+) (> 93%), with variable levels of CD4 and CD8 expression. All were HLA-DR+ (> 80%) with some HLA-DQ expression. Conjunctival T-cell lines from atopic Keratoconjunctivitis produced selective increases in LFN-gamma, IL-10 and IL-13 (P<0.01), those from vernal Keratoconjunctivitis produced increased IL-5 (P<0.01) whereas T-cell lines from giant papillary conjunctivitis produced only low levels of cytokines. IL-4 was only detected at the mRNA level and was expressed in four out of five T-cell lines in the vernal Keratoconjunctivitis group. In contrast there was moderate to strong expression of IFN-gamma in five out of six T-cell lines in atopic Keratoconjunctivitis.Conclusion Different patterns of T-cell cytokine profiles were observed for each disease, with low-level, non-polarized cytokine production in giant papillary conjunctivitis, a TH2-like profile in vernal Keratoconjunctivitis and a shift towards a TH1-like profile in atopic Keratoconjunctivitis.
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phenotypic characterization of t cells infiltrating the conjunctiva in chronic allergic eye disease
The Journal of Allergy and Clinical Immunology, 1996Co-Authors: D Metz, Annette S Bacon, Stephen T Holgate, Susan LightmanAbstract:Abstract BACKGROUND: Chronic allergic conjunctivitis comprises a spectrum of diseases including atopic Keratoconjunctivitis, atopic blepharoconjunctivitis, vernal Keratoconjunctivitis, and contact lens–associated giant papillary conjunctivitis. Each condition is characterized by a complex immunopathology with a mixed cellular infiltrate. Treatment with conventional mast cell stabilizers is often unsatisfactory, and therapy depends heavily on topical corticosteroids. OBJECTIVE: Recent evidence suggests that T lymphocytes play an important role in mediating the immunopathology seen in the chronic allergic response in the skin and the lungs. METHODS: We have therefore investigated the prevalence of T cells and their subsets in the different chronic allergic eye conditions by means of immunohistochemistry. RESULTS: We found significantly increased numbers of CD4 + , CD45RO + , and HLA-DR + T cells in the conjunctiva of patients with atopic and vernal Keratoconjunctivitis and giant papillary conjunctivitis, with a corresponding upregulation of markers present on antigen presenting cells. CONCLUSIONS: These findings suggest that like allergic conditions in the skin and lungs, CD4 + memory T cells are involved in the regulation of the immunopathology of chronic allergic eye responses. Further characterization of these T cells will provide the information necessary for future immunotherapeutic interventions. (J ALLERGY CLIN IMMUNOL 1996;98:686-96.)
Minmuh Sheu - One of the best experts on this subject based on the ideXlab platform.
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human adenovirus type 8 epidemic Keratoconjunctivitis with large corneal epithelial full layer detachment an endemic outbreak with uncommon manifestations
Clinical Ophthalmology, 2015Co-Authors: Yuehchang Lee, Nancy Chen, Itsong Huang, Huihua Yang, Chinte Huang, Likuang Chen, Minmuh SheuAbstract:Epidemic viral conjunctivitis is a highly contagious disease that is encountered year-round. The causative agents are mainly adenoviruses and enteroviruses. It occurs most commonly upon infection with subgroup D adenoviruses of types 8, 19, or 37. For common corneal involvement of human adenovirus type 8 epidemic Keratoconjunctivitis, full-layer epithelial detachment is rarely seen. Herein, we report three cases of epidemic Keratoconjunctivitis during an outbreak which manifested as large corneal epithelial full-layer detachment within a few days. The lesions healed without severe sequelae under proper treatment. The unique manifestation of this outbreak may indicate the evolution of human adenovirus type 8.
M.v. Meireles - One of the best experts on this subject based on the ideXlab platform.
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Ceratoconjuntivite por Encephalitozoon hellem em periquitos agapornis (Agapornis spp.) no Brasil: relato de caso
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2010Co-Authors: A.a. Nakamura, C.g. Homem, S.d. Garcia, M.v. MeirelesAbstract:A clinical case of Keratoconjunctivitis by Encephalitozoon hellem in adult lovebirds (Agapornis spp.) from a commercial flock is reported. Five animals presented clinical symptoms of Keratoconjunctivitis, blepharospasm, and bilateral blepharoedema, with seropurulent secretion. Coproparasitological diagnosis was carried out in fecal samples, with negative results. Two animals were necropsied, with detection of spores and other developmental stages of Microsporidium in conjunctival smears. The confirmation of the diagnosis was accomplished by the polimerase chain reaction with specific primers for 18S subunit of the rRNA of E. hellem, followed by sequencing of amplified fragments, which revealed 100% of genetic similarity to E. hellem. This study is the first report of E. hellem infection in birds in Brazil.
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Ceratoconjuntivite por Encephalitozoon hellem em periquitos agapornis (Agapornis spp.) no Brasil: relato de caso (Keratoconjunctivitis by Encephalitozoon hellem in lovebirds (Agapornis spp.) in Brazil: case report)
2010Co-Authors: A.a. Nakamura, C.g. Homem, S.d. Garcia, M.v. MeirelesAbstract:A clinical case of Keratoconjunctivitis by Encephalitozoon hellem in adult lovebirds (Agapornis spp.) from a commercial flock is reported. Five animals presented clinical symptoms of Keratoconjunctivitis, blepharospasm, and bilateral blepharoedema, with seropurulent secretion. Coproparasitological diagnosis was carried out in fecal samples, with negative results. Two animals were necropsied, with detection of spores and other developmental stages of Microsporidium in conjunctival smears. The confirmation of the diagnosis was accomplished by the polimerase chain reaction with specific primers for 18S subunit of the rRNA of E. hellem, followed by sequencing of amplified fragments, which revealed 100% of genetic similarity to E. hellem. This study is the first report of E. hellem infection in birds in Brazil.
Per Montan - One of the best experts on this subject based on the ideXlab platform.
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spontaneous corneal perforation associated with atopic Keratoconjunctivitis a case series and literature review
Acta Ophthalmologica, 2015Co-Authors: Emma Nivenius, Per MontanAbstract:Purpose To describe spontaneous corneal perforations in patients with atopic Keratoconjunctivitis. Methods Records of patients with atopic Keratoconjunctivitis and spontaneous corneal perforation seen by the authors were reviewed. A literature search of corneal complications in atopic Keratoconjunctivitis and predisposing factors for spontaneous corneal perforations in corneal ectasias was undertaken. Results Three patients with seven incidents of corneal perforation were identified and presented. Corneal thinning and keratoconus are observed at a higher rate in patients with atopic Keratoconjunctivitis, and severe ocular allergy has been reported as a risk factor for corneal hydrops. Conclusions Atopic Keratoconjunctivitis appears to be a risk factor for spontaneous sterile corneal perforation. Corneal thinning and keratoconus in addition to inflammatory changes of the cornea in these patients probably predispose to the corneal melt in these perforations. A close observation of the fellow eye of affected patients is important and systemic immunosuppressive therapy should be considered.
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Eosinophil cationic protein in tears in allergic conjunctivitis.
British Journal of Ophthalmology, 1996Co-Authors: Per Montan, Marianne Van Hage-hamstenAbstract:AIMS/BACKGROUND--Eosinophil cationic protein (ECP) is a quantifiable product secreted by activated eosinophils. The aim of this study was to assess the degree of eosinophil activity in different clinical stages of various forms of allergic conjunctivitis. METHODS--Tears were collected in glass capillary tubes from 14 subjects with seasonal allergic conjunctivitis (SAC), 23 subjects with vernal Keratoconjunctivitis (VKC), 16 subjects with atopic Keratoconjunctivitis (AKC), 10 subjects with giant papillary conjunctivitis (GPC), and 16 healthy control subjects. The samples were analysed in duplicate with a radio-immunoassay for ECP. RESULTS--Statistically significant differences were evident between healthy controls and allergic subjects (p
Esen K Akpek - One of the best experts on this subject based on the ideXlab platform.
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ocular surface disease associated with dupilumab treatment for atopic diseases
Ocular Surface, 2021Co-Authors: Canan Asli Utine, Penny A. Asbell, Esen K Akpek, Stephen C. PflugfelderAbstract:Dupilumab is the first US FDA approved biologic for treatment of atopic dermatitis. It is a human monoclonal antibody which blocks the shared receptor component, the interleukin (IL)-4α subunit, of IL-4 and IL-13 signaling pathways. Occurrence of "conjunctivitis", mostly in atopic dermatitis trials, has been the main side effect reported thus far. The etiology of "conjunctivitis" associated with dupilumab treatment is unclear and might be similar to atopic Keratoconjunctivitis. There is evidence in the published literature that unlike the Th2-like profile in vernal Keratoconjunctivitis, Th1-mediated inflammation is predominant in atopic Keratoconjunctivitis. Blocking the Th2 pathway with dupilumab therapy might result in a shift towards Th1, causing the ocular findings associated with dupilumab. In addition, blockage of IL-13 might have implications with regards to mucin production and ocular surface health. This review highlights the clinical manifestations, reviews treatment options and offers explanations for pathogenesis of this ocular surface diseases associated with dupilumab treatment.
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Immunopathological Features of Severe Chronic Atopic Keratoconjunctivitis and Effects of Topical Cyclosporine Treatment.
Ocular immunology and inflammation, 2018Co-Authors: Canan Asli Utine, Michael E. Stern, Esen K AkpekAbstract:ABSTRACTPurpose: To assess differential roles of inflammatory cells in pathophysiology of severe atopic Keratoconjunctivitis (AKC) and evaluate immunomodulatory effects of topical cyclosporine A (C...
