The Experts below are selected from a list of 318 Experts worldwide ranked by ideXlab platform
Subhash C. Pandey - One of the best experts on this subject based on the ideXlab platform.
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effects of estrogen treatment on expression of brain derived neurotrophic factor and camp response element binding protein expression and phosphorylation in rat amygdaloid and hippocampal structures
Neuroendocrinology, 2005Co-Authors: Jin Zhou, Huaibo Zhang, Rochelle S Cohen, Subhash C. PandeyAbstract:Clinical studies indicate an effect of estrogen (E2) on affect and cognition, which may be mediated by the cyclic AMP response element-binding protein (CREB) pathway and CREB-related gene target brain-derived neurotrophic factor (BDNF). We investigated the effect of E2 on CREB expression and phosphorylation and BDNF expression in the amygdala and hippocampus, areas involved in emotional processing. Ovariectomized rats were given 10 μg 17β-estradiol or vehicle for 14 days and expression of components of the CREB signaling pathway, i. e., CREB, phosphorylated CREB (pCREB), and BDNF in amygdala and hippocampus were investigated using immunogold labeling. Levels of BDNF mRNA were determined by in situ reverse-transcriptase polymerase chain reaction. We also examined the effect of E2 on CaMK IV Immunolabeling in the hippocampus and repeated our study on the effect of E2 on CaMK IV in amygdala. E2 increased Immunolabeling and mRNA levels of BDNF in the medial and basomedial amygdala and CA1 and CA3 regions of the hippocampus, but not in any other amygdaloid or hippocampal regions examined. E2 increased Immunolabeling of CaMK IV, CREB and pCREB in the medial and basomedial, but not central or basolateral, amygdala. E2 also increased CaMK IV and pCREB Immunolabeling in the CA1 and CA3 regions, but not CA2 region or dentate gyrus, of the hippocampus. There was no change in Immunolabeling of CREB in any hippocampal region. These data identify a signaling pathway through which E2 increases BDNF expression that may underlie some actions of E2 on affective behavior and indicate neuroanatomical heterogeneity in the E2 effect within the amygdala and hippocampus.
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Acute and Chronic Ethanol Consumption Effects on the Immunolabeling of Gq/11α Subunit Protein and Phospholipase C Isozymes in the Rat Brain
Journal of neurochemistry, 2002Co-Authors: Subhash C. PandeyAbstract:The goal of this investigation was to examine whether postreceptor sites [Gq/11 protein and phospholipase C (PLC) isozymes] of the phosphoinositide signal transduction system are involved in neuroadaptational mechanisms in the brain during chronic ethanol consumption. It was observed that acute ethanol treatment has no effect on the Immunolabeling of PLC-beta 1, -gamma 1, and -delta 1 and the alpha subunit of Gq/11 protein in the rat cortex as determined by western blotting using specific monoclonal antibodies. On the other hand, chronic ethanol consumption (15 days) resulted in a significant decrease in the Immunolabeling of PLC-beta 1, whereas under identical conditions, the Immunolabeling of PLC-gamma 1 and -delta 1 isozymes was not significantly altered. The decreased Immunolabeling of PLC-beta 1 during chronic ethanol consumption was not altered by 24 h of withdrawal after 15 days of ethanol consumption. The Immunolabeling of the alpha subunit of Gq/11 protein was significantly decreased after 15 days of ethanol consumption but had returned to normal levels after 24 h of ethanol withdrawal. Also, chronic ethanol treatment resulted in a significant decrease in phosphatidylinositol 4,5-bisphosphate-specific PLC activity, which remained the same after 24 h of ethanol withdrawal. These results suggest that decreased PLC activity during ethanol consumption and its withdrawal may be due to decreased protein levels of the Gq/11 protein-coupled PLC-beta 1 isozyme but not the PLC-gamma 1 or -delta 1 isozyme in the rat cortex. It is possible that changes in the protein levels of the Gq/11 protein-coupled PLC-beta 1 isozyme and in PLC activity in the brain may be involved in the cellular adaptation to chronic ethanol exposure.
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Cellular localization of serotonin2A (5HT2A) receptors in the rat brain
Brain research bulletin, 2000Co-Authors: Subhash C. PandeyAbstract:The serotonin(2A) (5HT(2A)) receptors have been shown to play an important role in several psychiatric disorders, including depression, schizophrenia, and alcoholism. This immunohistochemical study examined the cellular localization of 5HT(2A) receptors in various rat brain structures (olfactory, striatum, cortex, hippocampus, and amygdala). The colocalization of 5HT(2A) receptors in astrocytes was performed by double-immunofluorescence staining of 5HT(2A) receptors and of glial fibrillary acidic protein (GFAP) using confocal laser microscopy. 5HT(2A) receptor Immunolabeling was observed in olfactory bulbs, neostriatum, hippocampus, amygdala, and neocortex. Somata and dendrites of pyramidal cells in the frontal cortex (layer V) were densely labeled with 5HT(2A) receptors. In several other brain structures (hippocampus, amygdala, striatum, olfactory structures), 5HT(2A) receptor Immunolabeling was found in cell bodies and processes of neurons. 5HT(2A) receptor Immunolabeling was also observed in GFAP-positive cells of the various brain structures we investigated (layers I/VI of the neocortex, corpus callosum, hippocampal fissure and hilus, and amygdala). These results indicate that 5HT(2A) receptors are expressed in neurons and astrocytes and suggest the possibility that not only neuronal but also glial 5HT(2A) receptors have functional implications in psychiatric disorders.
Alan Fine - One of the best experts on this subject based on the ideXlab platform.
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ultrastructural distribution of the α7 nicotinic acetylcholine receptor subunit in rat hippocampus
The Journal of Neuroscience, 2001Co-Authors: Ruth Fabianfine, Paul Skehel, Emanuele Sher, Alan Fine, Heather A Davies, M L Errington, Michael G StewartAbstract:Acetylcholine (ACh) is an important neurotransmitter in the mammalian brain; it is implicated in arousal, learning, and other cognitive functions. Recent studies indicate that nicotinic receptors contribute to these cholinergic effects, in addition to the established role of muscarinic receptors. In the hippocampus, where cholinergic involvement in learning and memory is particularly well documented, 7 nicotinic acetylcholine receptor subunits (7 nAChRs) are highly expressed, but their precise ultrastructural localization has not been determined. Here, we describe the results of immunogold labeling of serial ultrathin sections through stratum radiatum of area CA1 in the rat. Using both anti-7 nAChR Immunolabeling and -bungarotoxin binding, we find that 7 nAChRs are present at nearly all synapses in CA1 stratum radiatum, with Immunolabeling present at both presynaptic and postsynaptic elements. Morphological considerations and double Immunolabeling indicate that GABAergic as well as glutamatergic synapses bear 7 nAChRs, at densities approaching those observed for glutamate receptors in CA1 stratum radiatum. Postsynaptically, 7 nAChRs often are distributed at dendritic spines in a perisynaptic annulus. In the postsynaptic cytoplasm, Immunolabeling is associated with spine apparatus and other membranous structures, suggesting that 7 nAChRs may undergo dynamic regulation, with insertion into the synapse and subsequent internalization. The widespread and substantial expression of 7 nAChRs at synapses in the hippocampus is consistent with an important role in mediating and/or modulating synaptic transmission, plasticity, and neurodegeneration.
Wayne L. Rickoll - One of the best experts on this subject based on the ideXlab platform.
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Postembedding Immunolabeling of Thin Sections of Drosophila Tissues for Transmission Electron Microscopy
Cold Spring Harbor protocols, 2012Co-Authors: Kent L. Mcdonald, David J. Sharp, Wayne L. RickollAbstract:Postembedding Immunolabeling using resin sections is the recommended method for beginners carrying out electron microscopy (EM) Immunolabeling. Postembedding labeling refers to labeling on sections, which is a method of gaining access to the interior of the cell without the harshness of detergent or ionic extraction as is performed with preembed labeling. Investigators already familiar with routine EM-sectioning techniques find EM Immunolabeling using resin sections easiest to do, as procedures are similar to those used when performing light microscopy (LM) Immunolabeling, but using a different resin. In addition, the overall preservation of structure is best in resin compared to use of cryosections or preembed labeling. The most critical component of immunoEM (iEM) is what primary antibody to use. This protocol descibes antibody labeling procedures for postembedding iEM using thin sections of Drosophila tissues.
Seungmo Hong - One of the best experts on this subject based on the ideXlab platform.
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desmin and cd31 Immunolabeling for detecting venous invasion of the pancreatobiliary tract cancers
PLOS ONE, 2020Co-Authors: Junyoung Shin, Laura D Wood, Ralph H Hruban, Seungmo HongAbstract:Although venous invasion (VI) is a poor prognostic factor for patients with pancreatobiliary tract cancers, its histopathologic characteristics have not been well described. We evaluated the patterns of VI and the added benefit provided by CD31, desmin, and dual CD31‒desmin Immunolabeling for identification of VI. We included 120 surgically resected pancreatobiliary tract cancer cases-59 cases as a test set with known VI and 61 cases as a validation set without information of VI. VI was classified into three patterns: intraepithelial neoplasia-like (IN-like), conventional, and destructive. Hematoxylin and eosin (H&E) staining and CD31, desmin, and dual CD31‒desmin Immunolabeling were performed. Foci number and patterns of VI were compared with the test and validation sets. More foci of VI were detected by single CD31 (P = 0.022) than H&E staining in the test set. CD31 Immunolabeling detected more foci of the conventional pattern of VI, and desmin Immunolabeling detected more foci of the destructive pattern (all, P < 0.001). Dual CD31‒desmin Immunolabeling identified more foci of VI (P = 0.012) and specifically detected more foci of IN-like (P = 0.045) and destructive patterns (P < 0.001) than H&E staining in the validation set. However, dual CD31‒desmin Immunolabeling was not helpful for detecting the conventional pattern of VI in the validation set. Patients with VI detected by dual CD31‒desmin Immunolabeling had shorter disease-free survival (P <0.001) than those without VI. VI detected by dual CD31‒desmin Immunolabeling was a worse prognostic indicator (P = 0.009). More foci of VI could be detected with additional single CD31 or dual CD31‒desmin Immunolabeling. The precise evaluation of VI with dual CD31‒desmin Immunolabeling can provide additional prognostic information for patients with surgically resected pancreatobiliary tract cancers.
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Desmin and CD31 Immunolabeling for detecting venous invasion of the pancreatobiliary tract cancers.
PloS one, 2020Co-Authors: Junyoung Shin, Laura D Wood, Ralph H Hruban, Seungmo HongAbstract:Although venous invasion (VI) is a poor prognostic factor for patients with pancreatobiliary tract cancers, its histopathologic characteristics have not been well described. We evaluated the patterns of VI and the added benefit provided by CD31, desmin, and dual CD31‒desmin Immunolabeling for identification of VI. We included 120 surgically resected pancreatobiliary tract cancer cases-59 cases as a test set with known VI and 61 cases as a validation set without information of VI. VI was classified into three patterns: intraepithelial neoplasia-like (IN-like), conventional, and destructive. Hematoxylin and eosin (H&E) staining and CD31, desmin, and dual CD31‒desmin Immunolabeling were performed. Foci number and patterns of VI were compared with the test and validation sets. More foci of VI were detected by single CD31 (P = 0.022) than H&E staining in the test set. CD31 Immunolabeling detected more foci of the conventional pattern of VI, and desmin Immunolabeling detected more foci of the destructive pattern (all, P < 0.001). Dual CD31‒desmin Immunolabeling identified more foci of VI (P = 0.012) and specifically detected more foci of IN-like (P = 0.045) and destructive patterns (P < 0.001) than H&E staining in the validation set. However, dual CD31‒desmin Immunolabeling was not helpful for detecting the conventional pattern of VI in the validation set. Patients with VI detected by dual CD31‒desmin Immunolabeling had shorter disease-free survival (P
Ruth Fabianfine - One of the best experts on this subject based on the ideXlab platform.
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ultrastructural distribution of the α7 nicotinic acetylcholine receptor subunit in rat hippocampus
The Journal of Neuroscience, 2001Co-Authors: Ruth Fabianfine, Paul Skehel, Emanuele Sher, Alan Fine, Heather A Davies, M L Errington, Michael G StewartAbstract:Acetylcholine (ACh) is an important neurotransmitter in the mammalian brain; it is implicated in arousal, learning, and other cognitive functions. Recent studies indicate that nicotinic receptors contribute to these cholinergic effects, in addition to the established role of muscarinic receptors. In the hippocampus, where cholinergic involvement in learning and memory is particularly well documented, 7 nicotinic acetylcholine receptor subunits (7 nAChRs) are highly expressed, but their precise ultrastructural localization has not been determined. Here, we describe the results of immunogold labeling of serial ultrathin sections through stratum radiatum of area CA1 in the rat. Using both anti-7 nAChR Immunolabeling and -bungarotoxin binding, we find that 7 nAChRs are present at nearly all synapses in CA1 stratum radiatum, with Immunolabeling present at both presynaptic and postsynaptic elements. Morphological considerations and double Immunolabeling indicate that GABAergic as well as glutamatergic synapses bear 7 nAChRs, at densities approaching those observed for glutamate receptors in CA1 stratum radiatum. Postsynaptically, 7 nAChRs often are distributed at dendritic spines in a perisynaptic annulus. In the postsynaptic cytoplasm, Immunolabeling is associated with spine apparatus and other membranous structures, suggesting that 7 nAChRs may undergo dynamic regulation, with insertion into the synapse and subsequent internalization. The widespread and substantial expression of 7 nAChRs at synapses in the hippocampus is consistent with an important role in mediating and/or modulating synaptic transmission, plasticity, and neurodegeneration.
