The Experts below are selected from a list of 17832 Experts worldwide ranked by ideXlab platform
Charles L Hoppel - One of the best experts on this subject based on the ideXlab platform.
-
mitochondrial carnitine palmitoyltransferase 1a cpt1a is part of an outer membrane fatty acid transfer complex
Journal of Biological Chemistry, 2011Co-Authors: Janos Kerner, Charles L HoppelAbstract:Abstract Carnitine palmitoyltransferase 1a (CPT1a) in liver mitochondrial outer membrane (MOM) catalyzes the primary regulated step in overall mitochondrial fatty acid oxidation. It has been suggested that the fundamental unit of CPT1a exists as a trimer, which under native conditions could form a dimer of the trimers creating a hexamer channel for acylcarnitine translocation. To examine the state of CPT1a in the MOM, we employed here a combined approach of sizing by mass and isolation using an Immunological Method. Blue Native electrophoresis (BNE) followed by detection with immunoblot and mass spectrometry identified large molecular weight complexes that contained not only CPT1a, but long-chain acyl-CoA synthetase (ACSL) and voltage-dependent anion channel (VDAC) as well. Immunoprecipitation with antisera against the proteins revealed a strong interaction between the three proteins. Immobilized CPT1a specific antibodies immunocaptured not only CPT1a, but also ACSL and VDAC, further strengthening findings with BNE and immunoprecipitation. This study shows strong protein-protein interaction between CPT1a, ACSL, and VDAC. We propose that this complex transfers activated fatty acids through the MOM.
-
mitochondrial carnitine palmitoyltransferase 1a cpt1a is part of an outer membrane fatty acid transfer complex
Journal of Biological Chemistry, 2011Co-Authors: Kwang Won Lee, Janos Kerner, Charles L HoppelAbstract:CPT1a (carnitine palmitoyltransferase 1a) in the liver mitochondrial outer membrane (MOM) catalyzes the primary regulated step in overall mitochondrial fatty acid oxidation. It has been suggested that the fundamental unit of CPT1a exists as a trimer, which, under native conditions, could form a dimer of the trimers, creating a hexamer channel for acylcarnitine translocation. To examine the state of CPT1a in the MOM, we employed a combined approach of sizing by mass and isolation using an Immunological Method. Blue native electrophoresis followed by detection with immunoblotting and mass spectrometry identified large molecular mass complexes that contained not only CPT1a but also long chain acyl-CoA synthetase (ACSL) and the voltage-dependent anion channel (VDAC). Immunoprecipitation with antisera against the proteins revealed a strong interaction between the three proteins. Immobilized CPT1a-specific antibodies immunocaptured not only CPT1a but also ACSL and VDAC, further strengthening findings with blue native electrophoresis and immunoprecipitation. This study shows strong protein-protein interaction between CPT1a, ACSL, and VDAC. We propose that this complex transfers activated fatty acids through the MOM.
Janos Kerner - One of the best experts on this subject based on the ideXlab platform.
-
mitochondrial carnitine palmitoyltransferase 1a cpt1a is part of an outer membrane fatty acid transfer complex
Journal of Biological Chemistry, 2011Co-Authors: Janos Kerner, Charles L HoppelAbstract:Abstract Carnitine palmitoyltransferase 1a (CPT1a) in liver mitochondrial outer membrane (MOM) catalyzes the primary regulated step in overall mitochondrial fatty acid oxidation. It has been suggested that the fundamental unit of CPT1a exists as a trimer, which under native conditions could form a dimer of the trimers creating a hexamer channel for acylcarnitine translocation. To examine the state of CPT1a in the MOM, we employed here a combined approach of sizing by mass and isolation using an Immunological Method. Blue Native electrophoresis (BNE) followed by detection with immunoblot and mass spectrometry identified large molecular weight complexes that contained not only CPT1a, but long-chain acyl-CoA synthetase (ACSL) and voltage-dependent anion channel (VDAC) as well. Immunoprecipitation with antisera against the proteins revealed a strong interaction between the three proteins. Immobilized CPT1a specific antibodies immunocaptured not only CPT1a, but also ACSL and VDAC, further strengthening findings with BNE and immunoprecipitation. This study shows strong protein-protein interaction between CPT1a, ACSL, and VDAC. We propose that this complex transfers activated fatty acids through the MOM.
-
mitochondrial carnitine palmitoyltransferase 1a cpt1a is part of an outer membrane fatty acid transfer complex
Journal of Biological Chemistry, 2011Co-Authors: Kwang Won Lee, Janos Kerner, Charles L HoppelAbstract:CPT1a (carnitine palmitoyltransferase 1a) in the liver mitochondrial outer membrane (MOM) catalyzes the primary regulated step in overall mitochondrial fatty acid oxidation. It has been suggested that the fundamental unit of CPT1a exists as a trimer, which, under native conditions, could form a dimer of the trimers, creating a hexamer channel for acylcarnitine translocation. To examine the state of CPT1a in the MOM, we employed a combined approach of sizing by mass and isolation using an Immunological Method. Blue native electrophoresis followed by detection with immunoblotting and mass spectrometry identified large molecular mass complexes that contained not only CPT1a but also long chain acyl-CoA synthetase (ACSL) and the voltage-dependent anion channel (VDAC). Immunoprecipitation with antisera against the proteins revealed a strong interaction between the three proteins. Immobilized CPT1a-specific antibodies immunocaptured not only CPT1a but also ACSL and VDAC, further strengthening findings with blue native electrophoresis and immunoprecipitation. This study shows strong protein-protein interaction between CPT1a, ACSL, and VDAC. We propose that this complex transfers activated fatty acids through the MOM.
Kwang Won Lee - One of the best experts on this subject based on the ideXlab platform.
-
mitochondrial carnitine palmitoyltransferase 1a cpt1a is part of an outer membrane fatty acid transfer complex
Journal of Biological Chemistry, 2011Co-Authors: Kwang Won Lee, Janos Kerner, Charles L HoppelAbstract:CPT1a (carnitine palmitoyltransferase 1a) in the liver mitochondrial outer membrane (MOM) catalyzes the primary regulated step in overall mitochondrial fatty acid oxidation. It has been suggested that the fundamental unit of CPT1a exists as a trimer, which, under native conditions, could form a dimer of the trimers, creating a hexamer channel for acylcarnitine translocation. To examine the state of CPT1a in the MOM, we employed a combined approach of sizing by mass and isolation using an Immunological Method. Blue native electrophoresis followed by detection with immunoblotting and mass spectrometry identified large molecular mass complexes that contained not only CPT1a but also long chain acyl-CoA synthetase (ACSL) and the voltage-dependent anion channel (VDAC). Immunoprecipitation with antisera against the proteins revealed a strong interaction between the three proteins. Immobilized CPT1a-specific antibodies immunocaptured not only CPT1a but also ACSL and VDAC, further strengthening findings with blue native electrophoresis and immunoprecipitation. This study shows strong protein-protein interaction between CPT1a, ACSL, and VDAC. We propose that this complex transfers activated fatty acids through the MOM.
Momar Ndao - One of the best experts on this subject based on the ideXlab platform.
-
the diagnosis of human fascioliasis by enzyme linked immunosorbent assay elisa using recombinant cathepsin l protease
PLOS Neglected Tropical Diseases, 2013Co-Authors: Bibiana Gonzales Santana, John P Dalton, Fabio Vasquez Camargo, Michael Parkinson, Momar NdaoAbstract:Background: Fascioliasis is a worldwide parasitic disease of domestic animals caused by helminths of the genus Fasciola. In many parts of the world, particularly in poor rural areas where animal disease is endemic, the parasite also infects humans. Adult parasites reside in the bile ducts of the host and therefore diagnosis of human fascioliasis is usually achieved by coprological examinations that search for parasite eggs that are carried into the intestine with the bile juices. However, these Methods are insensitive due to the fact that eggs are released sporadically and may be missed in low-level infections, and fasciola eggs may be misclassified as other parasites, leading to problems with specificity. Furthermore, acute clinical symptoms as a result of parasites migrating to the bile ducts appear before the parasite matures and begins egg laying. A human immune response to Fasciola antigens occurs early in infection. Therefore, an Immunological Method such as ELISA may be a more reliable, easy and cheap means to diagnose human fascioliasis than coprological analysis. Methodology/Principal findings: Using a panel of serum from Fasciola hepatica-infected patients and from uninfected controls we have optimized an enzyme-linked immunosorbent assay (ELISA) which employs a recombinant form of the major F. hepatica cathepsin L1 as the antigen for the diagnosis of human fascioliasis. We examined the ability of the ELISA test to discern fascioliasis from various other helminth and non-helminth parasitic diseases. Conclusions/Significance: A sensitive and specific fascioliasis ELISA test has been developed. This test is rapid and easy to use and can discriminate fasciola-infected individuals from patients harbouring other parasites with at least 99.9% sensitivity and 99.9% specificity. This test will be a useful standardized Method not only for testing individual samples but also in mass screening programs to assess the extent of human fascioliasis in regions where this zoonosis is suspected.
Čermáková Zdeňka - One of the best experts on this subject based on the ideXlab platform.
-
Cultivation of model parasite liver fluke (Fasciola hepatica) in domestic sheep (Ovis aries) - time behavior and results
Univerzita Karlova Farmaceutická fakulta v Hradci Králové, 2018Co-Authors: Čermáková ZdeňkaAbstract:Univerzita Karlova v Praze Farmaceutická fakulta v Hradci Králové Katedra farmakologie a toxikologie Student/ka: Zdeňka Čermáková Školitel: Prof. RNDr. Jiří Lamka, CSc. Název diplomové práce: Průběh a výsledky kultivace modelového parazita motolice jaterní (Fasciola hepatica) v ovci domácí (Ovis aries) Fasciolóza je parazitární onemocnění způsobené motolicí jaterní (Fasciola hepatica). Tato parazitóza je významným onemocněním hospodářských zvířat, volně žijící zvěře a v některých částech světa i člověka. Působí snížení užitkovosti a plodnosti zvířat, úbytky hmotnosti a vysoké ekonomické ztráty v chovech. Cílem této práce bylo detailně popsat průběh experimentální infekce ovcí infikovaných metacerkáriemi F. hepatica. Konkrétně byly sledovány změny v krevním obrazu, vylučování vajíček trusem a odpovědi imunitního systému nakažených jedinců. Zjištěné hodnoty byly porovnány s již provedenými studiemi. Pro průkaz fasciolózy u experimentálně nakažených ovcí byla využita koprologická sedimentační metoda, diferenciální stanovení leukocytů z krevních nátěrů, stanovení hematokritu a imunologická metoda ELISA (enzyme-linked immunosorbent assays). K experimentu byly použity tři jehnice plemene Texel infikované 200 metacerkáriemi. Diferenciální diagnostika leukocytů poskytla nejčasnější průkaz o průběhu a stavu...Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Zdeňka Čermáková Supervisor: Prof. RNDr. Jiří Lamka, CSc. Title of diploma thesis: The progression and results of the model parasite culturing liver fluke (Fasciola hepatica) in sheep (Ovis aries) Fasciolosis is a parasitic disease caused by liver fluke (Fasciola hepatica). This parasitosis is a significant disease in livestock, wild animals and in some parts of the world in humans. It causes a decrease in livestock performance and fertility, weight loss and high economic losses in livestock. The aim of this work was to describe in detail the course of artificial infection of sheep infected with F. hepatica metacercaria. Specifically, changes in blood count, egg excretion, and response of the immune system of infected individuals were monitored. The observed values were compared with the studies already performed. The demonstration of fasciolosis in artificially infected sheep was performed using the coprological sedimentation Method, the differential determination of leukocytes from the blood layers, the determination of hematocrit and the Immunological Method ELISA (enzyme-linked immunosorbent assays). Three lambs of Texel breeds infected with 200 metacercaria were used for the...Katedra farmakologie a toxikologieDepartment of Pharmacology and ToxicologyFaculty of Pharmacy in Hradec KrálovéFarmaceutická fakulta v Hradci Králov
-
Cultivation of model parasite liver fluke (Fasciola hepatica) in domestic sheep (Ovis aries) - time behavior and results
2018Co-Authors: Čermáková ZdeňkaAbstract:Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Zdeňka Čermáková Supervisor: Prof. RNDr. Jiří Lamka, CSc. Title of diploma thesis: The progression and results of the model parasite culturing liver fluke (Fasciola hepatica) in sheep (Ovis aries) Fasciolosis is a parasitic disease caused by liver fluke (Fasciola hepatica). This parasitosis is a significant disease in livestock, wild animals and in some parts of the world in humans. It causes a decrease in livestock performance and fertility, weight loss and high economic losses in livestock. The aim of this work was to describe in detail the course of artificial infection of sheep infected with F. hepatica metacercaria. Specifically, changes in blood count, egg excretion, and response of the immune system of infected individuals were monitored. The observed values were compared with the studies already performed. The demonstration of fasciolosis in artificially infected sheep was performed using the coprological sedimentation Method, the differential determination of leukocytes from the blood layers, the determination of hematocrit and the Immunological Method ELISA (enzyme-linked immunosorbent assays). Three lambs of Texel breeds infected with 200 metacercaria were used for the..
