Immunological Technique

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Nobuhisa Koga - One of the best experts on this subject based on the ideXlab platform.

  • evaluation of the mineralizable organic nitrogen in soil using an Immunological Technique
    Biology and Fertility of Soils, 2008
    Co-Authors: Shingo Matsumoto, Yohichi Koyama, Katsumasa Iijima, Rumiko Kodashima, Mari Hirata, Junko Kasuga, Nobuhisa Koga
    Abstract:

    Phosphate buffer-extractable organic nitrogen (PEON), which can be extracted from soil with a 67-mM neutral phosphate buffer solution, is considered to be a major source of mineralizable nitrogen (N) that is ubiquitous in soil. We have developed a Western blot analysis and enzyme-linked immune sorbent assay (ELISA) method for determining PEON. First, anti-PEON antibody (IgG) from a rabbit was produced using purified PEON from a volcanic ash soil. PEON was detected as a ladder-like staining pattern in the high-molecular-weight region and as a single band under 14 kDa in Western blot analysis using the anti-PEON IgG. In the ELISA assay, the anti-PEON IgG could specifically react with PEON irrespective of soil type and management. Furthermore, the absorbance at 450 nm in ELISA was highly correlated to the concentration of organic N (Norg) in phosphate extracts of different soils. We propose the immune assay using the anti-PEON IgG as a possible novel method for evaluating available N in soil.

Shingo Matsumoto - One of the best experts on this subject based on the ideXlab platform.

  • detection of soil organic nitrogen in xylem sap collected from nonmycorrhizal plants using an Immunological Technique
    Communications in Soil Science and Plant Analysis, 2012
    Co-Authors: Taiichiro Yoshida, Junko Kasuga, Junghyen Park, Mani Rajkumar, Shingo Matsumoto
    Abstract:

    Phosphate buffer–extractable organic nitrogen (PEON) is considered to be a ubiquitous high-molecular-weightnitrogen (N) compound in soil and may be an important N source for some plant species. We examined whether nonmycorrhizal plants qing-geng-cai (Brassica rapa var. chinensis) and spinach (Spinacia oleracea L. cv. Atras) could uptake PEON from the soilusing animmunoassay that was previously developed from a PEON determination method using anti-PEON IgG. Xylem sap analysis of spinach using an enzyme-linked immune sorbent assay (ELISA) showed that this plant hadhigh capabilities to take up PEON from the soil. Spinach and qing-geng-cai exuded substantial amounts of oxalate or citrate under inorganic N-deficient conditions, indicating exudation of organic acids is probably involved in PEON mobilization. Further results of immunohistochemical localization of PEON in the root section of qing-geng-cai revealed that intact or at least a fraction of PEON absorbed by the epidermis passes through the cortex cell ...

  • evaluation of the mineralizable organic nitrogen in soil using an Immunological Technique
    Biology and Fertility of Soils, 2008
    Co-Authors: Shingo Matsumoto, Yohichi Koyama, Katsumasa Iijima, Rumiko Kodashima, Mari Hirata, Junko Kasuga, Nobuhisa Koga
    Abstract:

    Phosphate buffer-extractable organic nitrogen (PEON), which can be extracted from soil with a 67-mM neutral phosphate buffer solution, is considered to be a major source of mineralizable nitrogen (N) that is ubiquitous in soil. We have developed a Western blot analysis and enzyme-linked immune sorbent assay (ELISA) method for determining PEON. First, anti-PEON antibody (IgG) from a rabbit was produced using purified PEON from a volcanic ash soil. PEON was detected as a ladder-like staining pattern in the high-molecular-weight region and as a single band under 14 kDa in Western blot analysis using the anti-PEON IgG. In the ELISA assay, the anti-PEON IgG could specifically react with PEON irrespective of soil type and management. Furthermore, the absorbance at 450 nm in ELISA was highly correlated to the concentration of organic N (Norg) in phosphate extracts of different soils. We propose the immune assay using the anti-PEON IgG as a possible novel method for evaluating available N in soil.

Junko Kasuga - One of the best experts on this subject based on the ideXlab platform.

  • detection of soil organic nitrogen in xylem sap collected from nonmycorrhizal plants using an Immunological Technique
    Communications in Soil Science and Plant Analysis, 2012
    Co-Authors: Taiichiro Yoshida, Junko Kasuga, Junghyen Park, Mani Rajkumar, Shingo Matsumoto
    Abstract:

    Phosphate buffer–extractable organic nitrogen (PEON) is considered to be a ubiquitous high-molecular-weightnitrogen (N) compound in soil and may be an important N source for some plant species. We examined whether nonmycorrhizal plants qing-geng-cai (Brassica rapa var. chinensis) and spinach (Spinacia oleracea L. cv. Atras) could uptake PEON from the soilusing animmunoassay that was previously developed from a PEON determination method using anti-PEON IgG. Xylem sap analysis of spinach using an enzyme-linked immune sorbent assay (ELISA) showed that this plant hadhigh capabilities to take up PEON from the soil. Spinach and qing-geng-cai exuded substantial amounts of oxalate or citrate under inorganic N-deficient conditions, indicating exudation of organic acids is probably involved in PEON mobilization. Further results of immunohistochemical localization of PEON in the root section of qing-geng-cai revealed that intact or at least a fraction of PEON absorbed by the epidermis passes through the cortex cell ...

  • evaluation of the mineralizable organic nitrogen in soil using an Immunological Technique
    Biology and Fertility of Soils, 2008
    Co-Authors: Shingo Matsumoto, Yohichi Koyama, Katsumasa Iijima, Rumiko Kodashima, Mari Hirata, Junko Kasuga, Nobuhisa Koga
    Abstract:

    Phosphate buffer-extractable organic nitrogen (PEON), which can be extracted from soil with a 67-mM neutral phosphate buffer solution, is considered to be a major source of mineralizable nitrogen (N) that is ubiquitous in soil. We have developed a Western blot analysis and enzyme-linked immune sorbent assay (ELISA) method for determining PEON. First, anti-PEON antibody (IgG) from a rabbit was produced using purified PEON from a volcanic ash soil. PEON was detected as a ladder-like staining pattern in the high-molecular-weight region and as a single band under 14 kDa in Western blot analysis using the anti-PEON IgG. In the ELISA assay, the anti-PEON IgG could specifically react with PEON irrespective of soil type and management. Furthermore, the absorbance at 450 nm in ELISA was highly correlated to the concentration of organic N (Norg) in phosphate extracts of different soils. We propose the immune assay using the anti-PEON IgG as a possible novel method for evaluating available N in soil.

Rumiko Kodashima - One of the best experts on this subject based on the ideXlab platform.

  • evaluation of the mineralizable organic nitrogen in soil using an Immunological Technique
    Biology and Fertility of Soils, 2008
    Co-Authors: Shingo Matsumoto, Yohichi Koyama, Katsumasa Iijima, Rumiko Kodashima, Mari Hirata, Junko Kasuga, Nobuhisa Koga
    Abstract:

    Phosphate buffer-extractable organic nitrogen (PEON), which can be extracted from soil with a 67-mM neutral phosphate buffer solution, is considered to be a major source of mineralizable nitrogen (N) that is ubiquitous in soil. We have developed a Western blot analysis and enzyme-linked immune sorbent assay (ELISA) method for determining PEON. First, anti-PEON antibody (IgG) from a rabbit was produced using purified PEON from a volcanic ash soil. PEON was detected as a ladder-like staining pattern in the high-molecular-weight region and as a single band under 14 kDa in Western blot analysis using the anti-PEON IgG. In the ELISA assay, the anti-PEON IgG could specifically react with PEON irrespective of soil type and management. Furthermore, the absorbance at 450 nm in ELISA was highly correlated to the concentration of organic N (Norg) in phosphate extracts of different soils. We propose the immune assay using the anti-PEON IgG as a possible novel method for evaluating available N in soil.

Mari Hirata - One of the best experts on this subject based on the ideXlab platform.

  • evaluation of the mineralizable organic nitrogen in soil using an Immunological Technique
    Biology and Fertility of Soils, 2008
    Co-Authors: Shingo Matsumoto, Yohichi Koyama, Katsumasa Iijima, Rumiko Kodashima, Mari Hirata, Junko Kasuga, Nobuhisa Koga
    Abstract:

    Phosphate buffer-extractable organic nitrogen (PEON), which can be extracted from soil with a 67-mM neutral phosphate buffer solution, is considered to be a major source of mineralizable nitrogen (N) that is ubiquitous in soil. We have developed a Western blot analysis and enzyme-linked immune sorbent assay (ELISA) method for determining PEON. First, anti-PEON antibody (IgG) from a rabbit was produced using purified PEON from a volcanic ash soil. PEON was detected as a ladder-like staining pattern in the high-molecular-weight region and as a single band under 14 kDa in Western blot analysis using the anti-PEON IgG. In the ELISA assay, the anti-PEON IgG could specifically react with PEON irrespective of soil type and management. Furthermore, the absorbance at 450 nm in ELISA was highly correlated to the concentration of organic N (Norg) in phosphate extracts of different soils. We propose the immune assay using the anti-PEON IgG as a possible novel method for evaluating available N in soil.