The Experts below are selected from a list of 4896 Experts worldwide ranked by ideXlab platform
Rui Henrique - One of the best experts on this subject based on the ideXlab platform.
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immune response and innervation signatures in aseptic hip Implant Loosening
Journal of Translational Medicine, 2016Co-Authors: Daniel M Vasconcelos, Manuel Ribeirodasilva, Antonio Mateus, Cecilia J Alves, Gil Costa Machado, Joana Machadosantos, Diogo Paramosdecarvalho, Ines S Alencastre, Rui HenriqueAbstract:Aseptic Loosening (AL) of hip prosthesis presents inflammation and pain as sign and symptom similarly to arthritis pathologies. Still, the immune and innervation profiles in hip AL remain unclear and their interplay is poorly explored. Herein, local tissue inflammatory response, sensory and sympathetic innervation as well as associated local mediators were assessed in hip joint microenvironment underlying AL and compared to osteoarthritis (OA). Histopathological analysis, immune cells (macrophages, T, B cells and PMNs) as well as sensory and sympathetic nerve fibers (SP+, CGRP+, TH+) distribution and profiles were analyzed on tissues retrieved from patients with failed hip prostheses due to AL (n = 20) and hip OA (n = 15) by immunohistochemistry. Additionally, transcriptional levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-12a, iNOS), anti-inflammatory cytokine (IL-10), osteoclastic factor (RANKL) and bone remodeling factor (TGF-β1) were locally evaluated by qRT-PCR. Serum TGF-β1 levels were assessed preoperatively by ELISA. Histopathological analysis revealed that tissues, aseptic interface membranes of AL patients had distinct tissue architecture and immune cells profile when compared to OA synovial tissues. Macrophages, T cells and B cells showed significant differences in tissue distribution. In OA, inflammation is mostly confined to the vicinity of synovial membrane while in AL macrophages infiltrated throughout the tissue. This differential immune profile is also accompanied with a distinct pattern of sensory and sympathetic innervation. Importantly, in AL patients, a lack of sympathetic innervation aseptic interface membranes without compensation mechanisms at cellular levels was observed with simultaneous reorganization of sensorial innervation. Despite the different histopathological portrait, AL and OA patients exhibited similar transcriptional levels of genes encoding key proteins in local immune response. Nevertheless, in both pathologies, TGF-β1 expression was prominent in sites where the inflammation is occurring. However, at systemic level no differences were found. These findings indicate that AL patients exhibit different local inflammatory response and innervation signatures from OA patients in hip joint. These insights shed the light on neuro-immune interplay in AL and highlight the need to better understand this crosstalk to unravel potential mechanisms for targeted-therapies to improve hip joint lifetime and treatment.
John D. Lucey - One of the best experts on this subject based on the ideXlab platform.
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An Implantable Wireless Inductive Sensor System Designed to Monitor Prosthesis Motion in Total Joint Replacement Surgery
IEEE Transactions on Biomedical Engineering, 2020Co-Authors: Hamed Mohammadbagherpoor, Paul Ierymenko, David E. Dausch, Edward Grant, Meghan H. Craver, Jim Carlson, John D. LuceyAbstract:Currently, the most common method for detecting prosthetic Implant Loosening is imaging. Unfortunately, imaging methods are imprecise in detecting the early signs of Implant Loosening. This paper describes a new wireless inductive proximity sensor system for detecting early Implant Loosening. The Loosening of the Implant is accurately detected by analyzing the electromagnetic field generated by the passive sensors located around the Implant. The sensor system was modeled and simulated using COMSOL, and then tested experimentally. The inductive proximity sensor and the metallic Implant form a coupled circuit is tuned to oscillate at a designed frequency. The circuit’s integrated controller measures and records specific sensor’s parameters such as resistance and inductance of the sensor that are directly related to the distance between the sensor system and the Implant. A prototype has been developed and the results show that the designed proximity sensor is capable of measuring the Loosening of the hip Implant at $\text{50}\ \mu$m resolution at distances of less than $\text{8 mm}$, and of $\text{100}\ \mu$m resolution at a distance of $\text{15 mm}$. Furthermore, there is a good correlation between the simulated and experimental results.
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An Implantable wireless inductive sensor system designed to monitor prosthesis motion in total joint replacement surgery
IEEE Transactions on Biomedical Engineering, 2019Co-Authors: Hamed Mohammadbagherpoor, Paul Ierymenko, Meghan Hegarty-craver, James B. Carlson, David E. Dausch, Edward Grant, John D. LuceyAbstract:Currently, the most common method for detecting prosthetic Implant Loosening is imaging. Unfortunately, imaging methods are imprecise in detecting the early signs of Implant Loosening. This paper describes a new wireless inductive proximity sensor system for detecting early Implant Loosening. The Loosening of the Implant is accurately detected by analyzing the electromagnetic field generated by the passive sensors located around the Implant. The sensor system was modeled and simulated using COMSOL, and then tested experimentally. The inductive proximity sensor and the metallic Implant form a coupled circuit is tuned to oscillate at a designed frequency. The circuit's integrated controller measures and records specific sensor's parameters such as resistance and inductance of the sensor that are directly related to the distance between the sensor system and the Implant. A prototype has been developed and the results show that the designed proximity sensor is capable of measuring the Loosening of the hip Implant at 50μm resolution at distances of less than 8mm, and of 100μm resolution at a distance of 15mm. Furthermore, there is a good correlation between the simulated and experimental results.
Chenhe Zhou - One of the best experts on this subject based on the ideXlab platform.
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sophocarpine attenuates wear particle induced Implant Loosening by inhibiting osteoclastogenesis and bone resorption via suppression of the nf κb signalling pathway in a rat model
British Journal of Pharmacology, 2018Co-Authors: Chenhe Zhou, Jiahong Meng, Bin Hu, Chenchen Zhao, Yute Yang, Wei Yu, Zexin Chen, Boon Chin Heng, Virginiajeni Akila ParkmanAbstract:Background and Purpose Aseptic prosthesis Loosening, caused by wear particles, is one of the most common causes of arthroplasty failure. Extensive and over-activated osteoclast formation and physiological functioning are regarded as the mechanism of prosthesis Loosening. Therapeutic modalities based on inhibiting osteoclast formation and bone resorption have been confirmed to be an effective way of preventing aseptic prosthesis Loosening. In this study, we have investigated the effects of sophocarpine (SPC, derived from Sophora flavescens) on preventing Implant Loosening and further explored the underlying mechanisms. Experimental Approach The effects of SPC in inhibiting osteoclastogenesis and bone resorption were evaluated in RANKL-induced osteoclast formation in vitro. A rat femoral particle-induced peri-Implant osteolysis model was established. Subsequently, micro-CT, histology, mechanical testing and bone turnover were used to assess the effects of SPC in preventing Implant Loosening. Key Results In vitro, we found that SPC suppressed osteoclast formation, bone resorption, F-actin ring formation and osteoclast-associated gene expression by inhibiting NF-κB signaling, specifically by targeting IκB kinases (IKKs). Our in vivo study showed that SPC prevented particle-induced prosthesis Loosening by inhibiting osteoclast formation, resulting in reduced periprosthetic bone loss, diminished pseudomembrane formation, improved bone-Implant contact, reduced bone resorption-related turnover, and enhanced stability of Implants. Inhibition of NF-κB signaling by SPC was further confirmed in vivo. Conclusion and Implications Our data demonstrated that SPC can prevent Implant Loosening through inhibiting osteoclast formation and bone resorption. Thus,SPC might be a novel therapeutic agent to prevent prosthesis Loosening and for osteolytic diseases.
Virginiajeni Akila Parkman - One of the best experts on this subject based on the ideXlab platform.
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sophocarpine attenuates wear particle induced Implant Loosening by inhibiting osteoclastogenesis and bone resorption via suppression of the nf κb signalling pathway in a rat model
British Journal of Pharmacology, 2018Co-Authors: Chenhe Zhou, Jiahong Meng, Bin Hu, Chenchen Zhao, Yute Yang, Wei Yu, Zexin Chen, Boon Chin Heng, Virginiajeni Akila ParkmanAbstract:Background and Purpose Aseptic prosthesis Loosening, caused by wear particles, is one of the most common causes of arthroplasty failure. Extensive and over-activated osteoclast formation and physiological functioning are regarded as the mechanism of prosthesis Loosening. Therapeutic modalities based on inhibiting osteoclast formation and bone resorption have been confirmed to be an effective way of preventing aseptic prosthesis Loosening. In this study, we have investigated the effects of sophocarpine (SPC, derived from Sophora flavescens) on preventing Implant Loosening and further explored the underlying mechanisms. Experimental Approach The effects of SPC in inhibiting osteoclastogenesis and bone resorption were evaluated in RANKL-induced osteoclast formation in vitro. A rat femoral particle-induced peri-Implant osteolysis model was established. Subsequently, micro-CT, histology, mechanical testing and bone turnover were used to assess the effects of SPC in preventing Implant Loosening. Key Results In vitro, we found that SPC suppressed osteoclast formation, bone resorption, F-actin ring formation and osteoclast-associated gene expression by inhibiting NF-κB signaling, specifically by targeting IκB kinases (IKKs). Our in vivo study showed that SPC prevented particle-induced prosthesis Loosening by inhibiting osteoclast formation, resulting in reduced periprosthetic bone loss, diminished pseudomembrane formation, improved bone-Implant contact, reduced bone resorption-related turnover, and enhanced stability of Implants. Inhibition of NF-κB signaling by SPC was further confirmed in vivo. Conclusion and Implications Our data demonstrated that SPC can prevent Implant Loosening through inhibiting osteoclast formation and bone resorption. Thus,SPC might be a novel therapeutic agent to prevent prosthesis Loosening and for osteolytic diseases.
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Sophocarpine attenuates wear particle‐induced Implant Loosening by inhibiting osteoclastogenesis and bone resorption via suppression of the NF‐κB signalling pathway in a rat model
British Journal of Pharmacology, 2018Co-Authors: Chen‐he Zhou, Jiahong Meng, Bin Hu, Chenchen Zhao, Yute Yang, Wei Yu, Boon Chin Heng, Ze‐xin Chen, Virginiajeni Akila ParkmanAbstract:Background and Purpose Aseptic prosthesis Loosening, caused by wear particles, is one of the most common causes of arthroplasty failure. Extensive and over-activated osteoclast formation and physiological functioning are regarded as the mechanism of prosthesis Loosening. Therapeutic modalities based on inhibiting osteoclast formation and bone resorption have been confirmed to be an effective way of preventing aseptic prosthesis Loosening. In this study, we have investigated the effects of sophocarpine (SPC, derived from Sophora flavescens) on preventing Implant Loosening and further explored the underlying mechanisms. Experimental Approach The effects of SPC in inhibiting osteoclastogenesis and bone resorption were evaluated in RANKL-induced osteoclast formation in vitro. A rat femoral particle-induced peri-Implant osteolysis model was established. Subsequently, micro-CT, histology, mechanical testing and bone turnover were used to assess the effects of SPC in preventing Implant Loosening. Key Results In vitro, we found that SPC suppressed osteoclast formation, bone resorption, F-actin ring formation and osteoclast-associated gene expression by inhibiting NF-κB signaling, specifically by targeting IκB kinases (IKKs). Our in vivo study showed that SPC prevented particle-induced prosthesis Loosening by inhibiting osteoclast formation, resulting in reduced periprosthetic bone loss, diminished pseudomembrane formation, improved bone-Implant contact, reduced bone resorption-related turnover, and enhanced stability of Implants. Inhibition of NF-κB signaling by SPC was further confirmed in vivo. Conclusion and Implications Our data demonstrated that SPC can prevent Implant Loosening through inhibiting osteoclast formation and bone resorption. Thus,SPC might be a novel therapeutic agent to prevent prosthesis Loosening and for osteolytic diseases.
Astrid D Bakker - One of the best experts on this subject based on the ideXlab platform.
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supraphysiological loading induces osteocyte mediated osteoclastogenesis in a novel in vitro model for bone Implant Loosening
Journal of Orthopaedic Research, 2018Co-Authors: Anna Fahlgren, Cornelia Bratengeier, C M Semeins, Jenneke Kleinnulend, Astrid D BakkerAbstract:We aimed to develop an in vitro model for bone Implant Loosening, allowing analysis of biophysical and biological parameters contributing to mechanical instability-induced osteoclast differentiatio ...
