Tissue Distribution

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Kunfeng Duan - One of the best experts on this subject based on the ideXlab platform.

  • Pharmacokinetics and Tissue Distribution study of orientin in rat by liquid chromatography.
    Journal of pharmaceutical and biomedical analysis, 2008
    Co-Authors: Qiao Wang, Zhi Fang Yuan, Lantong Zhang, Yang Cui, Kunfeng Duan
    Abstract:

    Abstract A simple HPLC–UV method was established for the determination of orientin in plasma and different Tissues of rat (heart, liver, spleen, lung, kidney, brain, stomach and small intestine). The separation was achieved by HPLC on a C18 column with a mobile phase composed of acetonitrile–0.1% acetic acid (20:80, v/v), UV detection was used at 348 nm. Good linearity was found between 0.250–50.0 μg/ml (r2 = 0.9966) for plasma samples and 0.050–50.0 μg/ml (r2 ≥ 0.9937) for the Tissue samples, respectively. Within- and between-day precisions expressed as the relative standard deviation (R.S.D.) for the method were 2.3–9.6% and 3.0–7.4%, respectively. The relative recoveries of orientin ranged from 95.4 to 100.6% for plasma and 93.1 to 107.9% for Tissue homogenates. The developed method was successfully applied to the pharmacokinetics and Tissue Distribution research after intravenous administration of a 20 mg/kg dose of orientin to healthy Sprague–Dawley rats. The main pharmacokinetics parameters obtained presented that orientin was quickly distributed and eliminated within 90 min after intravenous administration. The Tissue Distribution results showed that liver, lung and kidney were the major Distribution Tissues of orientin in rats, and that orientin had difficulty in crossing the blood–brain barrier. It was also found that there was no long-term accumulation of orientin in rat Tissues.

Karl-heinz Glatting - One of the best experts on this subject based on the ideXlab platform.

  • TissueDistributionDBs: a repository of organism-specific Tissue-Distribution profiles
    Theoretical Chemistry Accounts, 2009
    Co-Authors: Sunitha Kogenaru, Coral Del Val, Agnes Hotz-wagenblatt, Karl-heinz Glatting
    Abstract:

    Tissue-Distribution profiles are crucial for understanding the characteristics of cells and Tissues in terms of their differential expression of genes. Most of the currently available resources for Tissue-Distribution profiles are either specialized for a few particular organisms, Tissue types and disease stages or do not consider the “Tissue ontology” levels for the calculation of the Tissue-Distribution profiles. Therefore, we have developed “TissueDistributionDBs”, a repository of Tissue-Distribution profiles based on the expressed sequence tags (ESTs) data extracted from the UniGene database by employing “Tissue Ontology” available at BRENDA. To overcome the occurrence of the natural language variations in the EST’s source Tissue-type terms, we have generated a “Tissue synonym library” and standardized these Tissue-type terms by cross-referencing to the controlled vocabulary for Tissue-type terms available at BRENDA “Tissue Ontology”. Furthermore, we have provided a quantitative expression for genes among the Tissue types at various anatomical levels by constructing “Tissue slims”. Concurrently, the expression among Tissue types is used for Tissue-Distribution calculations. The resulting output profiles can be queried by the Sequence Retrieval System (SRS) and are currently available for 20 different model organisms. We benchmarked our database system against the Swissprot database using a set of 40 different Tissue types. This database system is useful for the understanding of the Tissue-specific expression patterns of genes, which have implications for the identification of possible new therapeutic drug targets, in gene discovery, and in the design and analysis of micro-arrays. TissueDistributionDBs can be accessed via the World Wide Web (www) at http://genius.embnet.dkfz-heidelberg.de/menu/Tissue_db/.

W H De Jong - One of the best experts on this subject based on the ideXlab platform.

  • the kinetics of the Tissue Distribution of silver nanoparticles of different sizes
    Biomaterials, 2010
    Co-Authors: Danille P K Lankveld, A G Oomen, Petra Krystek, Arianne M Neigh, Troost A De Jong, Cornelle W Noorlander, J C H Van Eijkeren, R E Geertsma, W H De Jong
    Abstract:

    Blood kinetics and Tissue Distribution of 20, 80 and 110 nm silver nanoparticles were investigated in rats up to 16 days after intravenous administration once daily for 5 consecutive days. Following both single and repeated injection, silver nanoparticles disappeared rapidly from the blood and distributed to all organs evaluated (liver, lungs, spleen, brain, heart, kidneys and testes) regardless of size. The 20 nm particles distributed mainly to liver, followed by kidneys and spleen, whereas the larger particles distributed mainly to spleen followed by liver and lung. In the other organs evaluated, no major differences between the sizes were observed. Size-dependent Tissue Distribution suggests size-dependent toxicity and health risks. Repeated administration resulted in accumulation in liver, lung and spleen, indicating that these organs may be potential target organs for toxicity after repeated exposure. A physiologically based pharmacokinetic (PBPK) model for nanoparticles which describes the kinetics of silver nanoparticles was developed. Model parameter values were estimated by fitting to data. No clear relation between parameter values and corresponding particle diameters became apparent.

Li Sha - One of the best experts on this subject based on the ideXlab platform.

  • Study on Tissue Distribution of nitrone derivative of tetramethylpyrazine in rats
    The Chinese Journal of Clinical Pharmacology, 2011
    Co-Authors: Li Sha
    Abstract:

    Objective To investigate the Tissue Distribution of nitrone derivative of tetramethylpyrazine(TMP),2-[[(1,1-dimethylethyl)oxidoimino]methyl]-3,5,6-trimethyl-pyrazine(TBN) in rats.Methods A validated HPLC-UV method was developed to assay the TBN concentration in plasma and Tissues so that to identify the Tissue Distribution behaviour.Purospher ODS C18 column(4.6 mm×150.0 mm,5 μm) was used for separation with methanol-0.05 mol·L-1 potassium dihydrogen phosphate solution(45∶55,pH 3.0) as mobile phase and 295 nm as detecting wavelength.Results After intravenous administration(80 mg·kg-1),the AUC0-360 of TBN in different Tissues ranged from 2.48~13.74 mg·min·g-1,the sequence from high to low was: plasmalungkidneyliverspleenbrainheart.The MRT0-360 was in the range of 129.50-161.95 min,the sequence from long to short was: lungspleenkidneybrainliverheartplasma.Conclusion A simple rapid HPLC method with high specificity and acceptable accuracy was developed.The Tissue Distribution information of TBN obtained by the HPLC method.The Tissue Distribution results of TBN showed that TBN distributed fast into Tissues including brain after intravenous administration.

Qiao Wang - One of the best experts on this subject based on the ideXlab platform.

  • Pharmacokinetics and Tissue Distribution study of orientin in rat by liquid chromatography.
    Journal of pharmaceutical and biomedical analysis, 2008
    Co-Authors: Qiao Wang, Zhi Fang Yuan, Lantong Zhang, Yang Cui, Kunfeng Duan
    Abstract:

    Abstract A simple HPLC–UV method was established for the determination of orientin in plasma and different Tissues of rat (heart, liver, spleen, lung, kidney, brain, stomach and small intestine). The separation was achieved by HPLC on a C18 column with a mobile phase composed of acetonitrile–0.1% acetic acid (20:80, v/v), UV detection was used at 348 nm. Good linearity was found between 0.250–50.0 μg/ml (r2 = 0.9966) for plasma samples and 0.050–50.0 μg/ml (r2 ≥ 0.9937) for the Tissue samples, respectively. Within- and between-day precisions expressed as the relative standard deviation (R.S.D.) for the method were 2.3–9.6% and 3.0–7.4%, respectively. The relative recoveries of orientin ranged from 95.4 to 100.6% for plasma and 93.1 to 107.9% for Tissue homogenates. The developed method was successfully applied to the pharmacokinetics and Tissue Distribution research after intravenous administration of a 20 mg/kg dose of orientin to healthy Sprague–Dawley rats. The main pharmacokinetics parameters obtained presented that orientin was quickly distributed and eliminated within 90 min after intravenous administration. The Tissue Distribution results showed that liver, lung and kidney were the major Distribution Tissues of orientin in rats, and that orientin had difficulty in crossing the blood–brain barrier. It was also found that there was no long-term accumulation of orientin in rat Tissues.