The Experts below are selected from a list of 53571 Experts worldwide ranked by ideXlab platform
Mohamed Faizal Abdulcareem - One of the best experts on this subject based on the ideXlab platform.
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In Ovo cpg dna delivery Increases Innate and adaptive immune cells In respiratory gastroIntestInal and immune systems post hatch correlatIng with lower Infectious laryngotracheitis virus Infection
PLOS ONE, 2018Co-Authors: Mohamed Sarjoon Abdulcader, Shayan Sharif, Éva Nagy, Aruna Amarasinghe, Victor Palominotapia, Hanaa Ahmedhassan, Khawaja Bakhtawar, Susantha Gomis, Mohamed Faizal AbdulcareemAbstract:CytosIne-guanosIne deoxynucleotides (CpG) DNA can be delivered In Ovo at embryo day (ED)18 for the stimulation of toll-like receptor (TLR)21 signalIng pathway that ultimately protects chickens agaInst a number of bacterial and viral Infections. There is a dearth of Information understandIng the mechanisms of protection Induced by In Ovo delivered CpG DNA. The objective of this study was to determIne the immune cell changes post-hatch followIng In Ovo delivery of the TLR21 ligand, CpG DNA. In order to quantify changes of percentage of KUL01+, IgM+ B, cluster of differentiation (CD)4+ and CD8α+ cells, trachea, lung, duodenum, large IntestIne, spleen and bursa of Fabricius were collected on day 1 post-hatch. We found Increased recruitments of KUL01+ cells, In organs of these body systems post-hatch followIng In Ovo delivery of CpG DNA. Although IgM+ B cells, CD4+ and CD8α+ cells were Increased In lungs and immune system organs, these cells were not quantifiable from the trachea, duodenum and large IntestIne immediately followIng the hatch. Furthermore, when CpG DNA is delivered In Ovo and subsequently Infected with Infectious laryngotracheitis virus (ILTV) post-hatch on day 1, CpG DNA reduces morbidity and mortality resultIng from ILTV Infection. This study provides Insights Into the mechanisms of host responses elicited followIng In Ovo delivery of CpG DNA In avian species.
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In Ovo delivery of toll like receptor 2 ligand lipoteichoic acid Induces pro Inflammatory mediators reducIng post hatch Infectious laryngotracheitis virus Infection
Veterinary Immunology and Immunopathology, 2015Co-Authors: Simrika Thapa, Éva Nagy, Mohamed Faizal AbdulcareemAbstract:Toll-like receptor (TLR) ligands are pathogen associated molecular patterns (PAMPs) recognized by the TLRs resultIng In Induction of host Innate immune responses. One of the PAMPs that bInds to TLR2 and cluster of differentiation (CD) 14 is lipotechoic acid (LTA), which activates downstream signals culmInatIng In the release of pro-Inflammatory cytokInes. In this study, we Investigated whether In Ovo LTA delivery leads to the Induction of antiviral responses agaInst post-hatch Infectious laryngotracheitis virus (ILTV) Infection. We first delivered the LTA Into embryo day (ED)18 eggs via In Ovo route so that the compound is available at the respiratory mucosa. Then the LTA treated and control ED18 eggs were allowed to hatch and the hatched chicken was Infected with ILTV Intratracheally on the day of hatch. We found that In Ovo delivered LTA reduces ILTV Infection post-hatch. We also found that In Ovo delivery of LTA significantly Increases mRNA expression of pro-Inflammatory mediators In pre-hatch embryo lungs as well as mononuclear cell Infiltration, predomInantly macrophages, In lung of post-hatch chickens. Altogether, the data suggest that In Ovo delivered LTA could be used to reduce ILTV Infection In newly hatched chickens.
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In Ovo delivery of cpg dna reduces avian Infectious laryngotracheitis virus Induced mortality and morbidity
Viruses, 2015Co-Authors: Simrika Thapa, Shayan Sharif, Éva Nagy, Mohamed Sarjoon Abdul Cader, K Murugananthan, Markus Czub, Mohamed Faizal AbdulcareemAbstract:Endosomal toll-like receptor-21 and -9 sense CpG DNA activatIng production of pro-Inflammatory mediators with antimicrobial effects. Here, we Investigated the Induction of antiviral response of In Ovo delivered CpG DNA agaInst Infectious laryngotracheitis virus (ILTV) Infection. We found that In Ovo delivered CpG DNA significantly reduces ILTV Infection pre-hatch correlatIng with the expression of IL-1β and Increase of macrophages In lungs. As assessed In vitro, CpG DNA stimulated avian macrophages could be a potential source of IL-1β and other pro-Inflammatory mediators. SInce we also found that In Ovo CpG DNA delivery maIntaIns Increased macrophages In the lungs post-hatch, we Infected the chickens on the day of hatch with ILTV. We found that In Ovo delivered CpG DNA significantly reduces mortality and morbidity resultIng from ILTV Infection encountered post-hatch. Thus, CpG DNA can be a candidate Innate immune stimulant worthy of further Investigation for the control of ILTV Infection In chickens.
Masaki Noda - One of the best experts on this subject based on the ideXlab platform.
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coordInated development of embryonic long bone on chorioallantoic membrane In Ovo prevents perichondrium derived suppressive signals agaInst cartilage growth
Bone, 2003Co-Authors: Yukiko Maeda, Masaki NodaAbstract:Perichondrium has been shown to elicit signals to suppress differentiation and proliferation of chondrocytes durIng endochondral bone formation based on In vitro organ culture [9]. However, these In vitro organ cultures did not allow the growth of bone collar, and thus the effect of perichondrium In a normal environment where development of adjacent embryonic tissues, IncludIng bone collar, is takIng place has not yet been fully understood. Therefore, we examIned the effect of perichondrium on cartilage development usIng chicken long bone organ cultures on chorioallantoic membrane In Ovo, which supported bone collar development. In contrast to previous observations In In vitro organ cultures, In Ovo organ cultures prevented overgrowth of epiphyseal cartilage due to the removal of perichondrium. This prevention was associated with the suppression of aggrecan gene expression In the absence of perichondrium In Ovo. These results Indicated that the perichondrium-derived activity that was observed In vitro to suppress cartilage development could be counterbalanced In Ovo, where culture conditions are closer to those In In vivo. TUNEL assay Indicated enhanced apoptosis In the presence of perichondrium In vitro, and removal of the perichondrium suppressed apoptosis. No major apoptosis was observed In Ovo regardless of the presence or the absence of perichondrium. Thus, chondrogenesis In long bone could be coordInately regulated through modulation of apoptosis by perichondrium and adjacent embryonic tissues, IncludIng bone collar, as revealed In In Ovo assay.
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coordInated development of embryonic long bone on chorioallantoic membrane In Ovo prevents perichondrium derived suppressive signals agaInst cartilage growth
Bone, 2003Co-Authors: Yukiko Maeda, Masaki NodaAbstract:Perichondrium has been shown to elicit signals to suppress differentiation and proliferation of chondrocytes durIng endochondral bone formation based on In vitro organ culture [9]. However, these In vitro organ cultures did not allow the growth of bone collar, and thus the effect of perichondrium In a normal environment where development of adjacent embryonic tissues, IncludIng bone collar, is takIng place has not yet been fully understood. Therefore, we examIned the effect of perichondrium on cartilage development usIng chicken long bone organ cultures on chorioallantoic membrane In Ovo, which supported bone collar development. In contrast to previous observations In In vitro organ cultures, In Ovo organ cultures prevented overgrowth of epiphyseal cartilage due to the removal of perichondrium. This prevention was associated with the suppression of aggrecan gene expression In the absence of perichondrium In Ovo. These results Indicated that the perichondrium-derived activity that was observed In vitro to suppress cartilage development could be counterbalanced In Ovo, where culture conditions are closer to those In In vivo. TUNEL assay Indicated enhanced apoptosis In the presence of perichondrium In vitro, and removal of the perichondrium suppressed apoptosis. No major apoptosis was observed In Ovo regardless of the presence or the absence of perichondrium. Thus, chondrogenesis In long bone could be coordInately regulated through modulation of apoptosis by perichondrium and adjacent embryonic tissues, IncludIng bone collar, as revealed In In Ovo assay.
Shayan Sharif - One of the best experts on this subject based on the ideXlab platform.
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Induction of immune response In chickens primed In Ovo with an Inactivated H9N2 avian Influenza virus vaccIne
BMC Research Notes, 2018Co-Authors: Jake Astill, Éva Nagy, Tamiru Alkie, Alexander Yitbarek, Khaled Taha-abdelaziz, Jegarubee Bavananthasivam, James John Petrik, Shayan SharifAbstract:Objective Infection of chickens with low pathogenic avian Influenza virus, such as H9N2 virus, culmInates In decreased egg production and Increased mortality and morbidity if co-Infection with other respiratory pathogens occurs. We have previously observed the Induction of antibody- and cell-mediated immune responses after Intramuscular admInistration of an H9N2 beta-propiolactone Inactivated virus vaccIne to chickens. Given the fact that In Ovo vaccInation represents a practical option for vaccInation agaInst H9N2 AIV In chickens, In the current study, we set out to characterize immune responses In chickens agaInst a beta-propiolactone Inactivated H9N2 virus vaccIne after primary vaccInation In Ovo on embryonic day 18, and secondary Intramuscular vaccInation on day 14 post-hatch. We also Included the Toll-like receptor 21 ligand, CpG ODN 2007, and an oil emulsion adjuvant, AddaVax™, as adjuvants for the vaccInes. Results Antibody-mediated immune responses were observed after admInisterIng the secondary Intramuscular vaccIne. Cell-mediated immune responses were observed In chickens that received the beta-propiolactone Inactivated H9N2 virus combIned with AddaVax™. Our results demonstrate that adaptive immune responses can be Induced In chickens after a primary In Ovo vaccInation and secondary Intramuscular vaccInation.
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In Ovo cpg dna delivery Increases Innate and adaptive immune cells In respiratory gastroIntestInal and immune systems post hatch correlatIng with lower Infectious laryngotracheitis virus Infection
PLOS ONE, 2018Co-Authors: Mohamed Sarjoon Abdulcader, Shayan Sharif, Éva Nagy, Aruna Amarasinghe, Victor Palominotapia, Hanaa Ahmedhassan, Khawaja Bakhtawar, Susantha Gomis, Mohamed Faizal AbdulcareemAbstract:CytosIne-guanosIne deoxynucleotides (CpG) DNA can be delivered In Ovo at embryo day (ED)18 for the stimulation of toll-like receptor (TLR)21 signalIng pathway that ultimately protects chickens agaInst a number of bacterial and viral Infections. There is a dearth of Information understandIng the mechanisms of protection Induced by In Ovo delivered CpG DNA. The objective of this study was to determIne the immune cell changes post-hatch followIng In Ovo delivery of the TLR21 ligand, CpG DNA. In order to quantify changes of percentage of KUL01+, IgM+ B, cluster of differentiation (CD)4+ and CD8α+ cells, trachea, lung, duodenum, large IntestIne, spleen and bursa of Fabricius were collected on day 1 post-hatch. We found Increased recruitments of KUL01+ cells, In organs of these body systems post-hatch followIng In Ovo delivery of CpG DNA. Although IgM+ B cells, CD4+ and CD8α+ cells were Increased In lungs and immune system organs, these cells were not quantifiable from the trachea, duodenum and large IntestIne immediately followIng the hatch. Furthermore, when CpG DNA is delivered In Ovo and subsequently Infected with Infectious laryngotracheitis virus (ILTV) post-hatch on day 1, CpG DNA reduces morbidity and mortality resultIng from ILTV Infection. This study provides Insights Into the mechanisms of host responses elicited followIng In Ovo delivery of CpG DNA In avian species.
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In Ovo delivery of cpg dna reduces avian Infectious laryngotracheitis virus Induced mortality and morbidity
Viruses, 2015Co-Authors: Simrika Thapa, Shayan Sharif, Éva Nagy, Mohamed Sarjoon Abdul Cader, K Murugananthan, Markus Czub, Mohamed Faizal AbdulcareemAbstract:Endosomal toll-like receptor-21 and -9 sense CpG DNA activatIng production of pro-Inflammatory mediators with antimicrobial effects. Here, we Investigated the Induction of antiviral response of In Ovo delivered CpG DNA agaInst Infectious laryngotracheitis virus (ILTV) Infection. We found that In Ovo delivered CpG DNA significantly reduces ILTV Infection pre-hatch correlatIng with the expression of IL-1β and Increase of macrophages In lungs. As assessed In vitro, CpG DNA stimulated avian macrophages could be a potential source of IL-1β and other pro-Inflammatory mediators. SInce we also found that In Ovo CpG DNA delivery maIntaIns Increased macrophages In the lungs post-hatch, we Infected the chickens on the day of hatch with ILTV. We found that In Ovo delivered CpG DNA significantly reduces mortality and morbidity resultIng from ILTV Infection encountered post-hatch. Thus, CpG DNA can be a candidate Innate immune stimulant worthy of further Investigation for the control of ILTV Infection In chickens.
Jen Hwey Chiu - One of the best experts on this subject based on the ideXlab platform.
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chrysIn suppresses il 6 Induced angiogenesis via down regulation of jak1 stat3 and vegf an In vitro and In Ovo approach
Journal of Agricultural and Food Chemistry, 2010Co-Authors: Kou-gi Shyu, Bao Wei Wang, Hang Chang, Yenhsu Chen, Jen Hwey ChiuAbstract:ChrysIn, 5,7-dihydroxyflavone, possesses many biologic properties. This study aimed to Investigate the effects and molecular mechanisms of chrysIn on IL-6-Induced angiogenesis In vitro and In Ovo. Chicken chorioallantoic membrane assay, an In Ovo angiogenesis assay, showed chrysIn significantly suppressed IL-6-Induced neovascularization. Furthermore, chrysIn significantly suppressed human umbilical veIn endothelial cell (HUVECs) migration and tube formation. The signalIng pathway Involved In chrysIn-related antiangiogenesis was also Investigated. The data Indicated that chrysIn is able to down-regulate the expression of glycoproteIn 130 (gp130), soluble IL-6 receptor (IL-6R), phosphorylated JAK1 and STAT3, and VEGF In HUVECs. The IL-6-Induced bIndIng of STAT3 was significantly suppressed by chrysIn. Moreover, chrysIn did not further suppress VEGF expression with STAT3 knocked down. Taken together, the results show that chrysIn suppresses IL-6-Induced angiogenesis through modulation of the sIL-6R/gp130/JAK...
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chrysIn suppresses il 6 Induced angiogenesis via down regulation of jak1 stat3 and vegf an In vitro and In Ovo approach
Journal of Agricultural and Food Chemistry, 2010Co-Authors: Chiu Mei Lin, Bao Wei Wang, Hang Chang, Yenhsu Chen, Kou-gi Shyu, Jen Hwey ChiuAbstract:ChrysIn, 5,7-dihydroxyflavone, possesses many biologic properties. This study aimed to Investigate the effects and molecular mechanisms of chrysIn on IL-6-Induced angiogenesis In vitro and In Ovo. Chicken chorioallantoic membrane assay, an In Ovo angiogenesis assay, showed chrysIn significantly suppressed IL-6-Induced neovascularization. Furthermore, chrysIn significantly suppressed human umbilical veIn endothelial cell (HUVECs) migration and tube formation. The signalIng pathway Involved In chrysIn-related antiangiogenesis was also Investigated. The data Indicated that chrysIn is able to down-regulate the expression of glycoproteIn 130 (gp130), soluble IL-6 receptor (IL-6R), phosphorylated JAK1 and STAT3, and VEGF In HUVECs. The IL-6-Induced bIndIng of STAT3 was significantly suppressed by chrysIn. Moreover, chrysIn did not further suppress VEGF expression with STAT3 knocked down. Taken together, the results show that chrysIn suppresses IL-6-Induced angiogenesis through modulation of the sIL-6R/gp130/JAK1/STAT3/VEGF signalIng pathway. ChrysIn may provide new therapeutic potential for IL-6-Induced pathological angiogenesis.
Éva Nagy - One of the best experts on this subject based on the ideXlab platform.
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Induction of immune response In chickens primed In Ovo with an Inactivated H9N2 avian Influenza virus vaccIne
BMC Research Notes, 2018Co-Authors: Jake Astill, Éva Nagy, Tamiru Alkie, Alexander Yitbarek, Khaled Taha-abdelaziz, Jegarubee Bavananthasivam, James John Petrik, Shayan SharifAbstract:Objective Infection of chickens with low pathogenic avian Influenza virus, such as H9N2 virus, culmInates In decreased egg production and Increased mortality and morbidity if co-Infection with other respiratory pathogens occurs. We have previously observed the Induction of antibody- and cell-mediated immune responses after Intramuscular admInistration of an H9N2 beta-propiolactone Inactivated virus vaccIne to chickens. Given the fact that In Ovo vaccInation represents a practical option for vaccInation agaInst H9N2 AIV In chickens, In the current study, we set out to characterize immune responses In chickens agaInst a beta-propiolactone Inactivated H9N2 virus vaccIne after primary vaccInation In Ovo on embryonic day 18, and secondary Intramuscular vaccInation on day 14 post-hatch. We also Included the Toll-like receptor 21 ligand, CpG ODN 2007, and an oil emulsion adjuvant, AddaVax™, as adjuvants for the vaccInes. Results Antibody-mediated immune responses were observed after admInisterIng the secondary Intramuscular vaccIne. Cell-mediated immune responses were observed In chickens that received the beta-propiolactone Inactivated H9N2 virus combIned with AddaVax™. Our results demonstrate that adaptive immune responses can be Induced In chickens after a primary In Ovo vaccInation and secondary Intramuscular vaccInation.
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In Ovo cpg dna delivery Increases Innate and adaptive immune cells In respiratory gastroIntestInal and immune systems post hatch correlatIng with lower Infectious laryngotracheitis virus Infection
PLOS ONE, 2018Co-Authors: Mohamed Sarjoon Abdulcader, Shayan Sharif, Éva Nagy, Aruna Amarasinghe, Victor Palominotapia, Hanaa Ahmedhassan, Khawaja Bakhtawar, Susantha Gomis, Mohamed Faizal AbdulcareemAbstract:CytosIne-guanosIne deoxynucleotides (CpG) DNA can be delivered In Ovo at embryo day (ED)18 for the stimulation of toll-like receptor (TLR)21 signalIng pathway that ultimately protects chickens agaInst a number of bacterial and viral Infections. There is a dearth of Information understandIng the mechanisms of protection Induced by In Ovo delivered CpG DNA. The objective of this study was to determIne the immune cell changes post-hatch followIng In Ovo delivery of the TLR21 ligand, CpG DNA. In order to quantify changes of percentage of KUL01+, IgM+ B, cluster of differentiation (CD)4+ and CD8α+ cells, trachea, lung, duodenum, large IntestIne, spleen and bursa of Fabricius were collected on day 1 post-hatch. We found Increased recruitments of KUL01+ cells, In organs of these body systems post-hatch followIng In Ovo delivery of CpG DNA. Although IgM+ B cells, CD4+ and CD8α+ cells were Increased In lungs and immune system organs, these cells were not quantifiable from the trachea, duodenum and large IntestIne immediately followIng the hatch. Furthermore, when CpG DNA is delivered In Ovo and subsequently Infected with Infectious laryngotracheitis virus (ILTV) post-hatch on day 1, CpG DNA reduces morbidity and mortality resultIng from ILTV Infection. This study provides Insights Into the mechanisms of host responses elicited followIng In Ovo delivery of CpG DNA In avian species.
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In Ovo delivery of toll like receptor 2 ligand lipoteichoic acid Induces pro Inflammatory mediators reducIng post hatch Infectious laryngotracheitis virus Infection
Veterinary Immunology and Immunopathology, 2015Co-Authors: Simrika Thapa, Éva Nagy, Mohamed Faizal AbdulcareemAbstract:Toll-like receptor (TLR) ligands are pathogen associated molecular patterns (PAMPs) recognized by the TLRs resultIng In Induction of host Innate immune responses. One of the PAMPs that bInds to TLR2 and cluster of differentiation (CD) 14 is lipotechoic acid (LTA), which activates downstream signals culmInatIng In the release of pro-Inflammatory cytokInes. In this study, we Investigated whether In Ovo LTA delivery leads to the Induction of antiviral responses agaInst post-hatch Infectious laryngotracheitis virus (ILTV) Infection. We first delivered the LTA Into embryo day (ED)18 eggs via In Ovo route so that the compound is available at the respiratory mucosa. Then the LTA treated and control ED18 eggs were allowed to hatch and the hatched chicken was Infected with ILTV Intratracheally on the day of hatch. We found that In Ovo delivered LTA reduces ILTV Infection post-hatch. We also found that In Ovo delivery of LTA significantly Increases mRNA expression of pro-Inflammatory mediators In pre-hatch embryo lungs as well as mononuclear cell Infiltration, predomInantly macrophages, In lung of post-hatch chickens. Altogether, the data suggest that In Ovo delivered LTA could be used to reduce ILTV Infection In newly hatched chickens.
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In Ovo delivery of cpg dna reduces avian Infectious laryngotracheitis virus Induced mortality and morbidity
Viruses, 2015Co-Authors: Simrika Thapa, Shayan Sharif, Éva Nagy, Mohamed Sarjoon Abdul Cader, K Murugananthan, Markus Czub, Mohamed Faizal AbdulcareemAbstract:Endosomal toll-like receptor-21 and -9 sense CpG DNA activatIng production of pro-Inflammatory mediators with antimicrobial effects. Here, we Investigated the Induction of antiviral response of In Ovo delivered CpG DNA agaInst Infectious laryngotracheitis virus (ILTV) Infection. We found that In Ovo delivered CpG DNA significantly reduces ILTV Infection pre-hatch correlatIng with the expression of IL-1β and Increase of macrophages In lungs. As assessed In vitro, CpG DNA stimulated avian macrophages could be a potential source of IL-1β and other pro-Inflammatory mediators. SInce we also found that In Ovo CpG DNA delivery maIntaIns Increased macrophages In the lungs post-hatch, we Infected the chickens on the day of hatch with ILTV. We found that In Ovo delivered CpG DNA significantly reduces mortality and morbidity resultIng from ILTV Infection encountered post-hatch. Thus, CpG DNA can be a candidate Innate immune stimulant worthy of further Investigation for the control of ILTV Infection In chickens.
