The Experts below are selected from a list of 80088 Experts worldwide ranked by ideXlab platform
Ida Skaar - One of the best experts on this subject based on the ideXlab platform.
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A fluorescence-based assay for In Vitro screenIng of Saprolegnia Inhibitors.
Journal of fish diseases, 2017Co-Authors: S E Ali, Ida SkaarAbstract:The Incidence of fish pathogenic oomycetes, Saprolegnia, has Increased significantly In aquaculture sInce the ban of malachite green. For the efficient characterization of anti-Saprolegnia therapeutics, simple accurate methods are required. However, the current screenIng methods are limited by time, and none of them are confirmIng the viability of treated spores or hyphae. In this study, a modified fluorescence-based assay for the In Vitro screenIng of Saprolegnia Inhibitors has been developed. This method Involves the use of FUN-1 viability dye combIned with calcofluor white M2R, and is based on the formation of orange-red cylIndrical Intravacuolar structures (CIVS) In metabolically active spores, hyphae and biofilms. Heat-killed and bronopol-treated Saprolegnia spores, hyphae and biofilms exhibited diffuse bright green fluorescence which confirms complete loss of viability. For boric acid-treated spores, no germInation was observed. However, tIny CIVS were observed In 50% of treated spores which Indicated reduction In their viability. Our results proved that FUN-1 dye is an efficient tool to distInguish between live and dead Saprolegnia spores, hyphae and biofilms and to monitor the change In Saprolegnia viability durIng qualitative evaluation of potential anti-Saprolegnia compounds.
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A simple In Vitro screenIng method to determIne the effects of drugs agaInst growth of Saprolegnia parasitica
Mycological Progress, 2005Co-Authors: Svein Stueland, Berit Tafjord Heier, Ida SkaarAbstract:To screen the effect of possible antifungal chemicals agaInst growth of fish pathogenic Saprolegnia spp., a simple and rapid In Vitro screenIng method has been developed. Heat sterilized hemp seeds ( Cannabis sativa ) colonized by Saprolegnia parasitica are exposed to different concentrations of the test drugs diluted In water. One Saprolegnia colonized hemp seed is transferred Into each well of a 48-well flat bottom tissue culture plate, after which 1 mL of each concentration of the test drugs is added per well. Subsequent to exposure and Incubation, the plate is Inspected and any Saprolegnia growth on the seeds is graded. The method described In the present paper proved to be simple, effective and reproducible In screenIng of fungistatic and fungicidal drugs agaInst Saprolegnia growth.
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A simple In Vitro screenIng method to determIne the effects of drugs agaInst growth of Saprolegnia parasitica
Mycological Progress, 2005Co-Authors: Svein Stueland, Berit T. Heier, Ida SkaarAbstract:To screen the effect of possible antifungal chemicals agaInst growth of fish pathogenic Saprolegnia spp., a simple and rapid In Vitro screenIng method has been developed. Heat sterilized hemp seeds (Cannabis sativa) colonized by Saprolegnia parasitica are exposed to different concentrations of the test drugs diluted In water. One Saprolegnia colonized hemp seed is transferred Into each well of a 48-well flat bottom tissue culture plate, after which 1 mL of each concentration of the test drugs is added per well. Subsequent to exposure and Incubation, the plate is Inspected and any Saprolegnia growth on the seeds is graded.
Svein Stueland - One of the best experts on this subject based on the ideXlab platform.
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A simple In Vitro screenIng method to determIne the effects of drugs agaInst growth of Saprolegnia parasitica
Mycological Progress, 2005Co-Authors: Svein Stueland, Berit Tafjord Heier, Ida SkaarAbstract:To screen the effect of possible antifungal chemicals agaInst growth of fish pathogenic Saprolegnia spp., a simple and rapid In Vitro screenIng method has been developed. Heat sterilized hemp seeds ( Cannabis sativa ) colonized by Saprolegnia parasitica are exposed to different concentrations of the test drugs diluted In water. One Saprolegnia colonized hemp seed is transferred Into each well of a 48-well flat bottom tissue culture plate, after which 1 mL of each concentration of the test drugs is added per well. Subsequent to exposure and Incubation, the plate is Inspected and any Saprolegnia growth on the seeds is graded. The method described In the present paper proved to be simple, effective and reproducible In screenIng of fungistatic and fungicidal drugs agaInst Saprolegnia growth.
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A simple In Vitro screenIng method to determIne the effects of drugs agaInst growth of Saprolegnia parasitica
Mycological Progress, 2005Co-Authors: Svein Stueland, Berit T. Heier, Ida SkaarAbstract:To screen the effect of possible antifungal chemicals agaInst growth of fish pathogenic Saprolegnia spp., a simple and rapid In Vitro screenIng method has been developed. Heat sterilized hemp seeds (Cannabis sativa) colonized by Saprolegnia parasitica are exposed to different concentrations of the test drugs diluted In water. One Saprolegnia colonized hemp seed is transferred Into each well of a 48-well flat bottom tissue culture plate, after which 1 mL of each concentration of the test drugs is added per well. Subsequent to exposure and Incubation, the plate is Inspected and any Saprolegnia growth on the seeds is graded.
N. Fischer - One of the best experts on this subject based on the ideXlab platform.
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by passIng In Vitro screenIng next generation sequencIng technologies applied to antibody display and In silico candidate selection
Nucleic Acids Research, 2010Co-Authors: U. Ravn, F. Gueneau, Loïc Baerlocher, Magne Osteras, M. Desmurs, P. Malinge, G. Magistrelli, Laurent Farinelli, M Koscovilbois, N. FischerAbstract:In recent years, unprecedented DNA sequencIng capacity provided by next generation sequencIng (NGS) has revolutionized genomic research. CombInIng the IllumIna sequencIng platform and a scFv library designed to confIne diversity to both CDR3, >1.9 × 10(7) sequences have been generated. This approach allowed for In depth analysis of the library's diversity, provided sequence Information on virtually all scFv durIng selection for bIndIng to two targets and a global view of these enrichment processes. UsIng the most frequent heavy chaIn CDR3 sequences, primers were designed to rescue scFv from the third selection round. Identification, based on sequence frequency, retrieved the most potent scFv and valuable candidates that were missed usIng classical In Vitro screenIng. Thus, by combInIng NGS with display technologies, laborious and time consumIng upfront screenIng can be by-passed or complemented and valuable Insights Into the selection process can be obtaIned to improve library design and understandIng of antibody repertoires.
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By-passIng In Vitro screenIng—next generation sequencIng technologies applied to antibody display and In silico candidate selection
Nucleic acids research, 2010Co-Authors: U. Ravn, F. Gueneau, Loïc Baerlocher, Magne Osteras, M. Desmurs, P. Malinge, G. Magistrelli, Laurent Farinelli, M.h. Kosco-vilbois, N. FischerAbstract:In recent years, unprecedented DNA sequencIng capacity provided by next generation sequencIng (NGS) has revolutionized genomic research. CombInIng the IllumIna sequencIng platform and a scFv library designed to confIne diversity to both CDR3, >1.9 × 10(7) sequences have been generated. This approach allowed for In depth analysis of the library's diversity, provided sequence Information on virtually all scFv durIng selection for bIndIng to two targets and a global view of these enrichment processes. UsIng the most frequent heavy chaIn CDR3 sequences, primers were designed to rescue scFv from the third selection round. Identification, based on sequence frequency, retrieved the most potent scFv and valuable candidates that were missed usIng classical In Vitro screenIng. Thus, by combInIng NGS with display technologies, laborious and time consumIng upfront screenIng can be by-passed or complemented and valuable Insights Into the selection process can be obtaIned to improve library design and understandIng of antibody repertoires.
Gi Jin Kim - One of the best experts on this subject based on the ideXlab platform.
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In Vitro screenIng system for hepatotoxicity: comparison of bone-marrow-derived mesenchymal stem cells and Placenta-derived stem cells.
Journal of cellular biochemistry, 2010Co-Authors: Hyun Jung Lee, Kyeung Eun Cha, Seong-gyu Hwang, Jin Kyeoung Kim, Gi Jin KimAbstract:Stem cells have unique properties such as self-renewal, plasticity to generate various cell types, and availability of cells of human origIn. The characteristics are attentive In the toxicity screenIng agaInst chemical toxicants. Placenta-derived stem cells (PDSCs) have been spotlighted as a new cell source In stem cell research recently because they are characterized by their capacity to differentiate Into multilIneages. However, the use of PDSCs as an In Vitro screenIng model for potential drug candidates has not yet been studied. Here, we analyzed the potentials for bone-marrow-derived mesenchymal stem (BM-MSCs), which is a representative adult stem cells and PDSCs as an In Vitro hepatotoxicity screenIng system, usIng well-known hepatotoxicants. BM-MSCs and PDSCs were analyzed to the potential for hepatogenic differentiation and were cultured with different concentrations of hepatotoxicants for time courses. The viability and ATP-bIndIng cassette (ABC) transporters were measured by the MTT assay and RT-PCR, respectively. The sensitivities of PDSCs to hepatotoxicants are more sensitive than those of BM-MSCs. The viability (IC50) to In PDSCs was less than that of BM-MSCs after 48 and 72 h (P
U. Ravn - One of the best experts on this subject based on the ideXlab platform.
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by passIng In Vitro screenIng next generation sequencIng technologies applied to antibody display and In silico candidate selection
Nucleic Acids Research, 2010Co-Authors: U. Ravn, F. Gueneau, Loïc Baerlocher, Magne Osteras, M. Desmurs, P. Malinge, G. Magistrelli, Laurent Farinelli, M Koscovilbois, N. FischerAbstract:In recent years, unprecedented DNA sequencIng capacity provided by next generation sequencIng (NGS) has revolutionized genomic research. CombInIng the IllumIna sequencIng platform and a scFv library designed to confIne diversity to both CDR3, >1.9 × 10(7) sequences have been generated. This approach allowed for In depth analysis of the library's diversity, provided sequence Information on virtually all scFv durIng selection for bIndIng to two targets and a global view of these enrichment processes. UsIng the most frequent heavy chaIn CDR3 sequences, primers were designed to rescue scFv from the third selection round. Identification, based on sequence frequency, retrieved the most potent scFv and valuable candidates that were missed usIng classical In Vitro screenIng. Thus, by combInIng NGS with display technologies, laborious and time consumIng upfront screenIng can be by-passed or complemented and valuable Insights Into the selection process can be obtaIned to improve library design and understandIng of antibody repertoires.
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By-passIng In Vitro screenIng—next generation sequencIng technologies applied to antibody display and In silico candidate selection
Nucleic acids research, 2010Co-Authors: U. Ravn, F. Gueneau, Loïc Baerlocher, Magne Osteras, M. Desmurs, P. Malinge, G. Magistrelli, Laurent Farinelli, M.h. Kosco-vilbois, N. FischerAbstract:In recent years, unprecedented DNA sequencIng capacity provided by next generation sequencIng (NGS) has revolutionized genomic research. CombInIng the IllumIna sequencIng platform and a scFv library designed to confIne diversity to both CDR3, >1.9 × 10(7) sequences have been generated. This approach allowed for In depth analysis of the library's diversity, provided sequence Information on virtually all scFv durIng selection for bIndIng to two targets and a global view of these enrichment processes. UsIng the most frequent heavy chaIn CDR3 sequences, primers were designed to rescue scFv from the third selection round. Identification, based on sequence frequency, retrieved the most potent scFv and valuable candidates that were missed usIng classical In Vitro screenIng. Thus, by combInIng NGS with display technologies, laborious and time consumIng upfront screenIng can be by-passed or complemented and valuable Insights Into the selection process can be obtaIned to improve library design and understandIng of antibody repertoires.
