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Meng Jin - One of the best experts on this subject based on the ideXlab platform.
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Therapeutic Potential of Human Adipose-Derived Stem Cell Exosomes In Stress UrInary IncontInence – An In Vitro and In Vivo Study
Karger Publishers, 2018Co-Authors: Yiwen Zhou, Xufeng Peng, Nailong Cao, Meng JinAbstract:Background/Aims: To evaluate whether local Injection of exosomes derived from human adipose-derived stem cells (hADSCs) facilitates recovery of stress urInary IncontInence (SUI) In a rat model. Methods: For the In vitro Study, a Cell CountIng Kit-8 (CCK-8) array and proteomic analysis were performed. For the In Vivo Study, female rats were divided Into four groups: sham, SUI, adipose-derived stem cell (ADSC), and exosomes (n = 12 each). The SUI model was generated by pudendal nerve transection and vagInal dilation. Vehicle, hADSCs, or exosomes were Injected Into the peripheral urethra. After 2, 4, and 8 weeks, the rats underwent cystometrography and leak poInt pressure (LPP) testIng, and tissues were harvested for histochemical analyses. Results: The CCK-8 experiment demonstrated that ADSC-derived exosomes could enhance the growth of skeletal muscle and Schwann cell lInes In a dose-dependent manner. Proteomic analysis revealed that ADSC-derived exosomes contaIned various proteIns of different signalIng pathways. Some of these proteIns are associated with the PI3K-Akt, Jak-STAT, and Wnt pathways, which are related to skeletal muscle and nerve regeneration and proliferation. In Vivo experiments illustrated that rats of the exosome group had higher bladder capacity and LPP, and had more striated muscle fibers and peripheral nerve fibers In the urethra than rats of the SUI group. Both urethral function and histology of rats In the exosome group were slightly better than those In the ADSC group. Conclusions: Local Injection of hADSC-derived exosomes improved functional and histological recovery after SUI
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Supplementary Table S2 for: Therapeutic potential of human adipose-derived stem cell exosomes In stress urInary IncontInence – an In vitro and In Vivo Study
2018Co-Authors: Yiwen Zhou, Xufeng Peng, Nailong Cao, Meng JinAbstract:The results of KEGG pathway analysisBackground/Aims: To evaluate whether local Injection of exosomes derived from human adipose-derived stem cells (hADSCs) facilitates recovery of stress urInary IncontInence (SUI) In a rat model. Methods: For the In vitro Study, a Cell CountIng Kit-8 (CCK-8) array and proteomic analysis were performed. For the In Vivo Study, female rats were divided Into four groups: sham, SUI, adipose-derived stem cell (ADSC), and exosomes (n = 12 each). The SUI model was generated by pudendal nerve transection and vagInal dilation. Vehicle, hADSCs, or exosomes were Injected Into the peripheral urethra. After 2, 4, and 8 weeks, the rats underwent cystometrography and leak poInt pressure (LPP) testIng, and tissues were harvested for histochemical analyses. Results: The CCK-8 experiment demonstrated that ADSC-derived exosomes could enhance the growth of skeletal muscle and Schwann cell lInes In a dose-dependent manner. Proteomic analysis revealed that ADSC-derived exosomes contaIned various proteIns of different signalIng pathways. Some of these proteIns are associated with the PI3K-Akt, Jak-STAT, and Wnt pathways, which are related to skeletal muscle and nerve regeneration and proliferation. In Vivo experiments illustrated that rats of the exosome group had higher bladder capacity and LPP, and had more striated muscle fibers and peripheral nerve fibers In the urethra than rats of the SUI group. Both urethral function and histology of rats In the exosome group were slightly better than those In the ADSC group. Conclusions: Local Injection of hADSC-derived exosomes improved functional and histological recovery after SUI
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Supplementary Table S1 for: Therapeutic potential of human adipose-derived stem cell exosomes In stress urInary IncontInence – an In vitro and In Vivo Study
2018Co-Authors: Yiwen Zhou, Xufeng Peng, Nailong Cao, Meng JinAbstract:Results of GO analysisBackground/Aims: To evaluate whether local Injection of exosomes derived from human adipose-derived stem cells (hADSCs) facilitates recovery of stress urInary IncontInence (SUI) In a rat model. Methods: For the In vitro Study, a Cell CountIng Kit-8 (CCK-8) array and proteomic analysis were performed. For the In Vivo Study, female rats were divided Into four groups: sham, SUI, adipose-derived stem cell (ADSC), and exosomes (n = 12 each). The SUI model was generated by pudendal nerve transection and vagInal dilation. Vehicle, hADSCs, or exosomes were Injected Into the peripheral urethra. After 2, 4, and 8 weeks, the rats underwent cystometrography and leak poInt pressure (LPP) testIng, and tissues were harvested for histochemical analyses. Results: The CCK-8 experiment demonstrated that ADSC-derived exosomes could enhance the growth of skeletal muscle and Schwann cell lInes In a dose-dependent manner. Proteomic analysis revealed that ADSC-derived exosomes contaIned various proteIns of different signalIng pathways. Some of these proteIns are associated with the PI3K-Akt, Jak-STAT, and Wnt pathways, which are related to skeletal muscle and nerve regeneration and proliferation. In Vivo experiments illustrated that rats of the exosome group had higher bladder capacity and LPP, and had more striated muscle fibers and peripheral nerve fibers In the urethra than rats of the SUI group. Both urethral function and histology of rats In the exosome group were slightly better than those In the ADSC group. Conclusions: Local Injection of hADSC-derived exosomes improved functional and histological recovery after SUI
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Supplementary Video S1 for: Therapeutic potential of human adipose-derived stem cell exosomes In stress urInary IncontInence – an In vitro and In Vivo Study
2018Co-Authors: Yiwen Zhou, Xufeng Peng, Nailong Cao, Meng JinAbstract:The video showed the Brownian activity of the hADSCs-derived exosome particles.Background/Aims: To evaluate whether local Injection of exosomes derived from human adipose-derived stem cells (hADSCs) facilitates recovery of stress urInary IncontInence (SUI) In a rat model. Methods: For the In vitro Study, a Cell CountIng Kit-8 (CCK-8) array and proteomic analysis were performed. For the In Vivo Study, female rats were divided Into four groups: sham, SUI, adipose-derived stem cell (ADSC), and exosomes (n = 12 each). The SUI model was generated by pudendal nerve transection and vagInal dilation. Vehicle, hADSCs, or exosomes were Injected Into the peripheral urethra. After 2, 4, and 8 weeks, the rats underwent cystometrography and leak poInt pressure (LPP) testIng, and tissues were harvested for histochemical analyses. Results: The CCK-8 experiment demonstrated that ADSC-derived exosomes could enhance the growth of skeletal muscle and Schwann cell lInes In a dose-dependent manner. Proteomic analysis revealed that ADSC-derived exosomes contaIned various proteIns of different signalIng pathways. Some of these proteIns are associated with the PI3K-Akt, Jak-STAT, and Wnt pathways, which are related to skeletal muscle and nerve regeneration and proliferation. In Vivo experiments illustrated that rats of the exosome group had higher bladder capacity and LPP, and had more striated muscle fibers and peripheral nerve fibers In the urethra than rats of the SUI group. Both urethral function and histology of rats In the exosome group were slightly better than those In the ADSC group. Conclusions: Local Injection of hADSC-derived exosomes improved functional and histological recovery after SUI
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Supplementary Figure S1 for: Therapeutic potential of human adipose-derived stem cell exosomes In stress urInary IncontInence – an In vitro and In Vivo Study
2018Co-Authors: Yiwen Zhou, Xufeng Peng, Nailong Cao, Meng JinAbstract:HE staInIng of mid-urethra 8 weeks after Injection showed no detectable Increase In Inflammatory cells around the Injection site In the ADSC and exosome groups compared with sham. The results Indicated that no significant immune response was Induced.Background/Aims: To evaluate whether local Injection of exosomes derived from human adipose-derived stem cells (hADSCs) facilitates recovery of stress urInary IncontInence (SUI) In a rat model. Methods: For the In vitro Study, a Cell CountIng Kit-8 (CCK-8) array and proteomic analysis were performed. For the In Vivo Study, female rats were divided Into four groups: sham, SUI, adipose-derived stem cell (ADSC), and exosomes (n = 12 each). The SUI model was generated by pudendal nerve transection and vagInal dilation. Vehicle, hADSCs, or exosomes were Injected Into the peripheral urethra. After 2, 4, and 8 weeks, the rats underwent cystometrography and leak poInt pressure (LPP) testIng, and tissues were harvested for histochemical analyses. Results: The CCK-8 experiment demonstrated that ADSC-derived exosomes could enhance the growth of skeletal muscle and Schwann cell lInes In a dose-dependent manner. Proteomic analysis revealed that ADSC-derived exosomes contaIned various proteIns of different signalIng pathways. Some of these proteIns are associated with the PI3K-Akt, Jak-STAT, and Wnt pathways, which are related to skeletal muscle and nerve regeneration and proliferation. In Vivo experiments illustrated that rats of the exosome group had higher bladder capacity and LPP, and had more striated muscle fibers and peripheral nerve fibers In the urethra than rats of the SUI group. Both urethral function and histology of rats In the exosome group were slightly better than those In the ADSC group. Conclusions: Local Injection of hADSC-derived exosomes improved functional and histological recovery after SUI
Karlthomas Wrbas - One of the best experts on this subject based on the ideXlab platform.
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comparison between two thermoplastic root canal obturation techniques regardIng extrusion of root canal fillIng a retrospective In Vivo Study
Clinical Oral Investigations, 2013Co-Authors: Christian Tennert, Ingo Lars Jungback, Karlthomas WrbasAbstract:The aim of this Study was to compare two different thermoplastic techniques—a core-carrier technique (Thermafil) and warm vertical compaction—In terms of overextension of root canal fillIng In Vivo. FlarIng of 88 teeth was conducted usIng Pro Files .04 as fInishIng files, and the teeth were obturated usIng Thermafil. FlarIng of 74 teeth was performed usIng Pro Files .06 as fInishIng files, and the teeth were obturated usIng warm vertical compaction. Seventy (80 %) of the teeth obturated usIng Thermafil and 31 (42 %) teeth obturated usIng warm vertical compaction show extruded root canal fillIng. In contrast to Thermafil, there is a higher rate of extruded root canal fillIng of teeth with more than one root canal usIng warm vertical compaction. Thermafil demonstrated a higher rate of extruded root canal fillIng compared to warm vertical compaction. Warm vertical compaction is a more predictable method of fillIng compared to Thermafil. Root canal fillIng extrusion will cause irritation of the surroundIng tissue and impair repair processes. In the present In Vivo Study, there was a higher rate of root canal fillIng extrusion usIng Thermafil compared to warm vertical compaction.
Paulo G Coelho - One of the best experts on this subject based on the ideXlab platform.
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the effect of platelet rich fibrIn exudate addition to porous poly lactic co glycolic acid scaffold In bone healIng an In Vivo Study
Journal of Biomedical Materials Research Part B, 2020Co-Authors: Lukasz Witek, Han Tian, Nick Tovar, Andrea Torroni, Rodrigo Neiva, Luiz Fernando Gil, Paulo G CoelhoAbstract:Bone graftIng procedures have been widely utilized as the current state-of-the-art for bone regeneration, with autogenous bone graft beIng the gold-standard bone reconstructive option. However, the use of autografts may be limited by secondary donor-site comorbidities, a fInite amount of donor supply, Increased operatIng time, and healthcare cost impact. Synthetic materials, or alloplasts, such as the polymeric material, poly(lactic-co-glycolic acid) (PLGA) has previously been utilized as a transient scaffold to support healIng of bone defects with the potential to locally delivery osteogenic additives. In this Study a novel procedure was adopted to Incorporate both the dissolved contents and mechanical components of leukocyte- and platelet-rich fibrIn (L-PRF) Into an PLGA scaffold through a two-step method: (a) extraction of the L-PRF membrane transudate with subsequent immersion of the PLGA scaffold In transudate followed by (b) deliverIng a fibrIn gel as a low-viscosity component that subsequently polymerizes Into a highly viscous, gel-like biological material withIn the pores of the PLGA scaffold. Two, ~0.40 cm3 , submandibular defects (n = 24) were created per side usIng rotary Instrumentation under contInuous irrigation In six sheep. Each site received a PLGA scaffold (Intra-Lock R&D, Boca Raton, FL), with one positive control (without L-PRF exudate addition [nL-PRF]), and one experimental (augmented with PLGA/L-PRF Blocks [L-PRF]). Animals were euthanized 6 weeks postoperatively and mandibles retrieved, en bloc, for histological analysis. Histomorphometric evaluation for bone regeneration was evaluated as bone area fraction occupancy (BAFO) withIn the region of Interest of the cortical bone (with specific image analysis software) and data presented as mean values with the correspondIng 95% confidence Interval values. Qualitative evaluation of nondecalcified histologic sections revealed extensive bone formation for both groups, with substantially more bone regeneration for the L-PRF Induced group relative nL-PRF group. Quantitative BAFO withIn the defect as function of the effect of L-PRF exudate on bone regeneration, demonstrated significantly (p = .018) higher values for the L-PRF group (38.26% ± 8.5%) relative to the nL-PRF group (~28% ± 4.0%). This In Vivo Study Indicated that L-PRF exudate has an impact on the regeneration of bone when Incorporated with the PLGA scaffold In a large translational model. Further studies are warranted In order to evaluate the L-PRF exudate added, as well as explorIng the preparation methods, In order to facilitate bone regeneration.
Peter Ngan - One of the best experts on this subject based on the ideXlab platform.
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comparison of bond strength between a conventional resIn adhesive and a resIn modified glass ionomer adhesive an In vitro and In Vivo Study
American Journal of Orthodontics and Dentofacial Orthopedics, 2004Co-Authors: Andrew Summers, Elizabeth Kao, Jeffrey Gilmore, Erdogan Gunel, Peter NganAbstract:The objectives of this Study were (1) to compare the In Vivo survival rates of orthodontic brackets bonded with a resIn-modified glass ionomer adhesive (Fuji Ortho LC; GC America, Alsip, Ill) after conditionIng with 10% polyacrylic acid and a conventional resIn adhesive (Light Bond; Reliance Orthodontic Products, Itasca, Ill) bonded with 37% phosphoric acid, (2) to compare the In vitro bond shear/peel bond strength between the 2 adhesives, (3) to determIne the mode of bracket failure In the In Vivo and In vitro tests accordIng to the adhesive remnant Index (ARI), and (4) to compare the changes In surface morphology of enamel surface after etchIng or conditionIng with 10% polyacrylic acid, with scannIng electron microscopy. In the In vitro Study, 50 extracted premolars were randomly divided Into 4 groups: brackets bonded with Fuji Ortho LC or Light Bond adhesive that were debonded after either 30 mInutes or 24 hours. Bond strengths were determIned with a testIng machIne at a crosshead speed of 1 mm/mIn. Data were analyzed with analysis of variance and a paired Student t test. The In Vivo Study consisted of 398 teeth that were randomly bonded with Fuji Ortho LC or Light Bond adhesive In 22 subjects with the split-mouth technique. Bracket survival rates and distribution were followed for 1.3 years. Data were analyzed with Kaplan-Meier product-limit estimates of surVivorship function. The In vitro Study results showed significant differences (P <.05) among the adhesives and the debond times. Light Bond had significantly greater bond strengths than Fuji Ortho LC at 24 hours (18.46 +/- 2.95 MPa vs 9.56 +/- 1.85 MPa) and 30 mInutes (16.19 +/- 2.04 MPa vs 6.93 +/- 1.93 MPa). Mean ARI scores showed that Fuji Ortho LC had significantly greater Incidences of enamel/adhesive failure than Light Bond adhesive (4.9 vs 4.1). For the In Vivo Study, no significant differences In failure rate, sex, or location In dental arch or ARI ratIngs were found between the 2 adhesives. These results suggest that, compared with conventional resIn, brackets bonded with resIn-modified glass ionomer adhesive had significantly less shear bond strength In vitro. However, similar survival rates of the 2 materials studied after 1.3 years Indicate that resIn-reInforced glass ionomers can provide adequate bond strengths clInically. The weaker chemical bondIng between the adhesive and the enamel might make it easier for clInicians to clean up adhesives on the enamel surface after debondIng.
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comparison of bond strength between a conventional resIn adhesive and a resIn modified glass ionomer adhesive an In vitro and In Vivo Study
American Journal of Orthodontics and Dentofacial Orthopedics, 2004Co-Authors: Andrew Summers, Jeffrey Gilmore, Erdogan Gunel, Peter NganAbstract:Abstract The objectives of this Study were (1) to compare the In Vivo survival rates of orthodontic brackets bonded with a resIn-modified glass ionomer adhesive (Fuji Ortho LC; GC America, Alsip, Ill) after conditionIng with 10% polyacrylic acid and a conventional resIn adhesive (Light Bond; Reliance Orthodontic Products, Itasca, Ill) bonded with 37% phosphoric acid, (2) to compare the In vitro bond shear/peel bond strength between the 2 adhesives, (3) to determIne the mode of bracket failure In the In Vivo and In vitro tests accordIng to the adhesive remnant Index (ARI), and (4) to compare the changes In surface morphology of enamel surface after etchIng or conditionIng with 10% polyacrylic acid, with scannIng electron microscopy. In the In vitro Study, 50 extracted premolars were randomly divided Into 4 groups: brackets bonded with Fuji Ortho LC or Light Bond adhesive that were debonded after either 30 mInutes or 24 hours. Bond strengths were determIned with a testIng machIne at a crosshead speed of 1 mm/mIn. Data were analyzed with analysis of variance and a paired Student t test. The In Vivo Study consisted of 398 teeth that were randomly bonded with Fuji Ortho LC or Light Bond adhesive In 22 subjects with the split-mouth technique. Bracket survival rates and distribution were followed for 1.3 years. Data were analyzed with Kaplan-Meier product-limit estimates of surVivorship function. The In vitro Study results showed significant differences (P
J. Hajo Van Bockel - One of the best experts on this subject based on the ideXlab platform.
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Ingrowth of aorta wall Into stent grafts impregnated with basic fibroblast growth factor: a porcIne In Vivo Study of blood vessel prosthesis healIng
Journal of vascular surgery, 2004Co-Authors: J Annemieke M Van Der Bas, Paul H A Quax, Arjen C Van Den Berg, M.j.t. Visser, Edwin Van Der Linden, J. Hajo Van BockelAbstract:Objective: Endovascular aneurysm repair is an alternative treatment of abdomInal aortic aneurysm. The procedure is less Invasive, and morbidity and most probably mortality are reduced. However, some problems, such as endoleakage, are yet to be resolved. Endoleakage can occur after graft migration, as a result of Insufficient fixation of the stent graft. One cause is deficient healIng between the aortic neck and the stent graft. We hypothesize that better healIng, achieved by Induction of vascular cell Ingrowth Into the graft material, results In better graft fixation. Previously we demonstrated Ingrowth of neoIntima Into the graft material if the stent graft is impregnated with a coat of basic fibroblast growth factor (bFGF), heparIn, and collagen. In this Study we evaluated healIng with bFGF-heparIn-collagen-coated stent grafts In Vivo. Methods: In 4 pigs, 32 endovascular stent grafts, manufactured from standard Dacron and Gianturco Z-stents, were placed In the aorta. The stent grafts were impregnated with either bFGF-heparIn contaInIng collagen (n = 16) or control collagen (n = 16). After 4 and 8 weeks animals were killed, and Ingrowth and healIng of the stent grafts were macroscopically and electron microscopically evaluated. Results: After 8 weeks all bFGF-impregnated stent grafts demonstrated Ingrowth of tissue and healIng between the graft and the aorta, whereas the control nonimpregnated stent grafts showed no Ingrowth. Microscopic evaluation demonstrated α-smooth muscle actIn-positive cells, most probably smooth muscle cells or myofibroblasts, growIng from the vascular wall through the graft material. Conclusion: A Dacron prosthesis impregnated with collagen, heparIn, and bFGF Induced graft healIng In an In Vivo pig model, In contrast to nonimpregnated stent grafts. This In Vivo Study confirms our previous fIndIngs In vitro. These results Indicate that healIng between Dacron and the aorta can be achieved, and suggest that type I endoleakage may be resolved by InducIng healIng between the aortic wall and the prosthesis with graft material contaInIng growth factor. Chemicals/CAS: atropIne, 51-55-8, 55-48-1; basic fibroblast growth factor, 106096-93-9; collagen, 9007-34-5; heparIn, 37187-54-5, 8057-48-5, 8065-01-8, 9005-48-5; isoflurane, 26675-46-7; nitric oxide, 10102-43-9; pancuronium bromide, 15500-66-0; thiopental, 71-73-8, 76-75-5; warfarIn, 129-06-6, 2610-86-8, 3324-63-8, 5543-58-8, 81-81-2; Coated Materials, Biocompatible; Collagen, 9007-34-5; Fibroblast Growth Factor 2, 103107-01-3; Growth Substances; HeparIn, 9005-49-6; Polyethylene Terephthalates
