The Experts below are selected from a list of 31155 Experts worldwide ranked by ideXlab platform
Wang Chunyao - One of the best experts on this subject based on the ideXlab platform.
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breeding and biological characteristics of curly hair mutation Inbred Strain rat
Chinese journal of veterinary science, 2009Co-Authors: Zhang Jintao, Bi Hongzheng, Zhu Kuicheng, Du Chunyan, Wang ChunyaoAbstract:curly rats were mated through brother-sister.The phenotypes and the mode of inheritance of curly rats were analyzed by test cross experiments.At the same time,its histological character,serum electrolyte contents and biochemical indexs,hematology indexs,main organ coefficients and growth were studied.Results showed that curly mutation was inherited in an autosomal semi-dominant manner and controlled by single dominant gene in the autosomes.No difference in the histological characters of skin between cury rats and SD rats,but curly rats hair follicles had changed.Serum electrolyte contents and biochemical and hematology indexs had little difference from SD rat's.Medium-term growth had differences from SD rat's.Spleen and thymus gland organ coefficients was less than SD rat's.All the results showed curly rat has clear phenotype and mode of inheritance,its biological characteristics are different from SD rat's,it will be a new laboratory animal model.
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Short tandem repeat in genetic checkup of Inbred Strain Yuyi hairless mice
Journal of Henan Medical University, 2003Co-Authors: Wang ChunyaoAbstract:Aim:To investigate the short tandem repeat (STR) loci polymorphism of Inbred Strain Yuyi hairless mice. Methods:Five STR loci on number 3,4,7,and 16 chromosomes were analyzed by PCR in Inbred Strain Yuyi hairless mice,DBA Inbred Strain mice,and Kunming mice. Results: Inbred Strain Yuyi hairless mice displayed single allelic gene band with five pairs of primers. Polymorphism was found at D7Nds1,D3Mit22,and D16Mit4 loci,but not at D3Mit21 and D4Mit2 loci.Conclusion: D7Nds1,D3Mit22,and D16Mit4 loci can be used in genetic checkup of Inbred Strain hairless mice.Combining with the results of skin transplant test, it is suggested that the Inbred Strain Yuyi hairless mice meet the request of Inbred Strain.
Liu Tian-fu - One of the best experts on this subject based on the ideXlab platform.
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Establishment of major biological indices of Shanyi colony Inbred Strain of Chinese hamster
Journal of Shanxi Medical University, 2009Co-Authors: Liu Tian-fuAbstract:Objective To establish the normal range of biological markers of Shanyi colony Inbred Strain of Chinese hamster. Methods The body weight and body length were measured in different week-old Shanyi colony Inbred Strain of Chinese hamsters,and growth curve fitting equation was established.The respiratory rate, blood pressure,and organ coefficients of adult male and female hamsters were determined. Results Logistic curve model was fitted well with growth curve of body weight and length of Shanyi colony Inbred Strain of Chinese hamster(R20.98).There was significant difference in respiratory rate between adult male and female hamsters(P0.05),but not in blood pressure.Except in liver and kidney,organ coefficients of female were higher than that of male in heart,spleen,lung,pancreas,stomach,eyeball,and brain(P0.05). Conclusion The biological markers of Shanyi colony Inbred Strain of Chinese hamster are successfully established,which will offer useful biological data for breeding and animal experiments of Shanyi colony Inbred Strain of Chinese hamster.
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RAPD Analysis of Shanyi Colony of Chinese Hamster Inbred E Genealogy
2004Co-Authors: Liu Tian-fuAbstract:ObjectiveTo analyze the genetic purity of Chinese hamsters E Genealogy Inbred Strain. Method31 RAPD primers were selected and used to amplify 12 sam ples of E genealogy of Chinese hamster. The amplified fragment bands were analy zed to calculate the similarity index.Result In this study ,the similarity index of all samples ranged from 94 3 1% to 99 78%, averaging at 97 49%. ConclusionChinese hamster of Shanyi colony E genealogy has reached a high degree of genetic purity.
Haiyang Wang - One of the best experts on this subject based on the ideXlab platform.
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novel enhanced gfp positive congenic Inbred Strain establishment and application of tumor bearing nude mouse model
Cancer Science, 2020Co-Authors: Qing Lan, Yanming Chen, Chungang Dai, Xifeng Fei, Jun Dong, Yanhua Shen, Xingliang Dai, Bing Liu, Qilong Wang, Haiyang WangAbstract:Transgenic GFP gene mice are widely used. Given the unique advantages of immunodeficient animals in the field of oncology research, we aim to establish a nude mouse Inbred Strain that stably expresses enhanced GFP (EGFP) for use in transplanted tumor microenvironment (TME) research. Female C57BL/6-Tg(CAG-EGFP) mice were backcrossed with male BALB/c nude mice for 11 generations. The genotype and phenotype of novel Inbred Strain Foxn1nu .B6-Tg(CAG-EGFP) were identified by biochemical loci detection, skin transplantation and flow cytometry. PCR and fluorescence spectrophotometry were performed to evaluate the relative expression of EGFP in different parts of the brain. Red fluorescence protein (RFP) gene was stably transfected into human glioma stem cells (GSC), SU3, which were then transplanted intracerebrally or ectopically into Foxn1nu .B6-Tg(CAG-EGFP) mice. Cell co-expression of EGFP and RFP in transplanted tissues was further analyzed with the Live Cell Imaging System (Cell'R, Olympus) and FISH. The Inbred Strain Foxn1nu .B6-Tg(CAG-EGFP) shows different levels of EGFP expression in brain tissue. The hematological and immune cells of the Inbred Strain mice were close to those of nude mice. EGFP was stably expressed in multiple sites of Foxn1nu .B6-Tg(CAG-EGFP) mice, including brain tissue. With the dual-fluorescence tracing transplanted tumor model, we found that SU3 induced host cell malignant transformation in TME, and tumor/host cell fusion. In conclusion, EGFP is differentially and widely expressed in brain tissue of Foxn1nu .B6-Tg(CAG-EGFP), which is an ideal model for TME investigation. With Foxn1nu .B6-Tg(CAG-EGFP) mice, our research demonstrated that host cell malignant transformation and tumor/host cell fusion play an important role in tumor progression.
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a novel enhanced gfp positive congenic Inbred Strain establishment and application of tumor bearing nude mouse model
Cancer Science, 2020Co-Authors: Yanming Chen, Jun Dong, Yanhua Shen, Qilong Wang, Haiyang Wang, Shenggang Li, Zhaohui Lu, Zhengyu Zhou, Xiaoyan Ji, Zhimin WangAbstract:Transgenic green fluorescent protein (GFP) gene mice are widely used. And for the unique advantages of immunodeficient animals in the field of oncology research, we aim to establish a nude mouse Inbred Strain, which stably expresses EGFP (enhanced-GFP) for the use of transplanted tumor microenvironment (TME) research. Female C57BL/6-Tg(CAG-EGFP) mice were backcrossed with male BALB/c nude mice for eleven generations. The genotype and phenotype of novel Inbred Strain Foxn1nu .B6-Tg(CAG-EGFP) were identified by biochemical loci detection, skin transplantation and flow cytometry. PCR and fluorescence spectrophotometry were performed to evaluate the relative expression of EGFP in different parts of the brain. Red fluorescence protein (RFP) gene was stably transfected into human glioma stem cells (GSCs) SU3, and then were transplanted intracerebrally or ectopic into Foxn1nu .B6-Tg(CAG-EGFP) mice. Cells co-expression of EGFP and RFP in transplanted tissues were further analyzed with Live Cell Imaging System and FISH. The Inbred Strain Foxn1nu .B6-Tg(CAG-EGFP) shows different levels of EGFP expression in brain tissue. The hematological and immune cells of the Inbred Strain mice were close to nude mice. EGFP was stably expressed in multiple sites of Foxn1nu .B6-Tg(CAG-EGFP) mice including brain tissue. With the dual-fluorescence tracing transplanted tumor model, we found that SU3 induced host cells malignant transformation in TME, and tumor/host cells fusion. In conclusion, EGFP is differentially and widely expressed in brain tissue of Foxn1nu .B6-Tg(CAG-EGFP), which is an ideal model for TME investigation. With Foxn1nu .B6-Tg(CAG-EGFP) mice, our research demonstrated that host cells malignant transformation and tumor/host cells fusion play an important role in tumor progression.
David H Kohn - One of the best experts on this subject based on the ideXlab platform.
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Inbred Strain specific effects of exercise in wild type and biglycan deficient mice
Annals of Biomedical Engineering, 2010Co-Authors: Joseph M Wallace, Kurtulus Golcuk, Michael D Morris, David H KohnAbstract:Biglycan (bgn)-deficient mice (KO) have defective osteoblasts which lead to changes in the amount and quality of bone. Altered tissue strength in C57BL6/129 (B6;129) KO mice, a property which is independent of tissue quantity, suggests that deficiencies in tissue quality are responsible. However, the response to bgn-deficiency is Inbred Strain-specific. Mechanical loading influences bone matrix quality in addition to any increase in bone mass or change in bone formation activity. Since many diseases influence the mechanical integrity of bone through altered tissue quality, loading may be a way to prevent and treat extracellular matrix deficiencies. C3H/He (C3H) mice consistently have a less vigorous response to mechanical loading vs. other Inbred Strains. It was therefore hypothesized that the bones from both wild type (WT) and KO B6;129 mice would be more responsive to exercise than the bones from C3H mice. To test these hypotheses at 11 weeks of age, following 21 consecutive days of exercise, we investigated cross-sectional geometry, mechanical properties, and tissue composition in the tibiae of male mice bred on B6;129 and C3H backgrounds. This study demonstrated Inbred Strain-specific compositional and mechanical changes following exercise in WT and KO mice, and showed evidence of genotype-specific changes in bone in response to loading in a gene disruption model. This study further shows that exercise can influence bone tissue composition and/or mechanical integrity without changes in bone geometry. Together, these data suggest that exercise may represent a possible means to alter tissue quality and mechanical deficiencies caused by many diseases of bone.
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Inbred Strain specific response to biglycan deficiency in the cortical bone of c57bl6 129 and c3h he mice
Journal of Bone and Mineral Research, 2009Co-Authors: Joseph M Wallace, Kurtulus Golcuk, Michael D Morris, David H KohnAbstract:Inbred Strain-specific differences in mice exist in bone cross-sectional geometry, mechanical properties, and indices of bone formation. Inbred Strain-specific responses to external stimuli also exist, but the role of background Strain in response to genetic deletion is not fully understood. Biglycan (bgn) deficiency impacts bone through negative regulation of osteoblasts, resulting in extracellular matrix alterations and decreased mechanical properties. Because osteoblasts from C3H/He (C3H) mice are inherently more active versus osteoblasts from other Inbred Strains, and the bones of C3H mice are less responsive to other insults, it was hypothesized that C3H mice would be relatively more resistant to changes associated with bgn deficiency compared with C57BL6/129 (B6;129) mice. Changes in mRNA expression, tissue composition, mineral density, bone formation rate, cross-sectional geometry, and mechanical properties were studied at 8 and 11 wk of age in the tibias of male wildtype and bgn-deficient mice bred on B6;129 and C3H background Strains. Bgn deficiency altered collagen cross-linking and gene expression and the amount and composition of mineral in vivo. In bgn's absence, changes in collagen were independent of mouse Strain. Bgn-deficiency increased the amount of mineral in both Strains, but changes in mineral composition, cross-sectional geometry, and mechanical properties were dependent on genetic background. Bgn deficiency influenced the amount and composition of bone in mice from both Strains at 8 wk, but C3H mice were better able to maintain properties close to wildtype (WT) levels. By 11 wk, most properties from C3H knockout (KO) bones were equal to or greater than WT levels, whereas phenotypic differences persisted in B6;129 KO mice. This is the first study into mouse Strain-specific changes in a small leucine-rich proteoglycan gene disruption model in properties across the bone hierarchy and is also one of the first to relate these changes to mechanical competence. This study supports the importance of genetic factors in determining the response to a gene deletion and defines biglycan's importance to collagen and mineral composition in vivo.
Yanming Chen - One of the best experts on this subject based on the ideXlab platform.
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novel enhanced gfp positive congenic Inbred Strain establishment and application of tumor bearing nude mouse model
Cancer Science, 2020Co-Authors: Qing Lan, Yanming Chen, Chungang Dai, Xifeng Fei, Jun Dong, Yanhua Shen, Xingliang Dai, Bing Liu, Qilong Wang, Haiyang WangAbstract:Transgenic GFP gene mice are widely used. Given the unique advantages of immunodeficient animals in the field of oncology research, we aim to establish a nude mouse Inbred Strain that stably expresses enhanced GFP (EGFP) for use in transplanted tumor microenvironment (TME) research. Female C57BL/6-Tg(CAG-EGFP) mice were backcrossed with male BALB/c nude mice for 11 generations. The genotype and phenotype of novel Inbred Strain Foxn1nu .B6-Tg(CAG-EGFP) were identified by biochemical loci detection, skin transplantation and flow cytometry. PCR and fluorescence spectrophotometry were performed to evaluate the relative expression of EGFP in different parts of the brain. Red fluorescence protein (RFP) gene was stably transfected into human glioma stem cells (GSC), SU3, which were then transplanted intracerebrally or ectopically into Foxn1nu .B6-Tg(CAG-EGFP) mice. Cell co-expression of EGFP and RFP in transplanted tissues was further analyzed with the Live Cell Imaging System (Cell'R, Olympus) and FISH. The Inbred Strain Foxn1nu .B6-Tg(CAG-EGFP) shows different levels of EGFP expression in brain tissue. The hematological and immune cells of the Inbred Strain mice were close to those of nude mice. EGFP was stably expressed in multiple sites of Foxn1nu .B6-Tg(CAG-EGFP) mice, including brain tissue. With the dual-fluorescence tracing transplanted tumor model, we found that SU3 induced host cell malignant transformation in TME, and tumor/host cell fusion. In conclusion, EGFP is differentially and widely expressed in brain tissue of Foxn1nu .B6-Tg(CAG-EGFP), which is an ideal model for TME investigation. With Foxn1nu .B6-Tg(CAG-EGFP) mice, our research demonstrated that host cell malignant transformation and tumor/host cell fusion play an important role in tumor progression.
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a novel enhanced gfp positive congenic Inbred Strain establishment and application of tumor bearing nude mouse model
Cancer Science, 2020Co-Authors: Yanming Chen, Jun Dong, Yanhua Shen, Qilong Wang, Haiyang Wang, Shenggang Li, Zhaohui Lu, Zhengyu Zhou, Xiaoyan Ji, Zhimin WangAbstract:Transgenic green fluorescent protein (GFP) gene mice are widely used. And for the unique advantages of immunodeficient animals in the field of oncology research, we aim to establish a nude mouse Inbred Strain, which stably expresses EGFP (enhanced-GFP) for the use of transplanted tumor microenvironment (TME) research. Female C57BL/6-Tg(CAG-EGFP) mice were backcrossed with male BALB/c nude mice for eleven generations. The genotype and phenotype of novel Inbred Strain Foxn1nu .B6-Tg(CAG-EGFP) were identified by biochemical loci detection, skin transplantation and flow cytometry. PCR and fluorescence spectrophotometry were performed to evaluate the relative expression of EGFP in different parts of the brain. Red fluorescence protein (RFP) gene was stably transfected into human glioma stem cells (GSCs) SU3, and then were transplanted intracerebrally or ectopic into Foxn1nu .B6-Tg(CAG-EGFP) mice. Cells co-expression of EGFP and RFP in transplanted tissues were further analyzed with Live Cell Imaging System and FISH. The Inbred Strain Foxn1nu .B6-Tg(CAG-EGFP) shows different levels of EGFP expression in brain tissue. The hematological and immune cells of the Inbred Strain mice were close to nude mice. EGFP was stably expressed in multiple sites of Foxn1nu .B6-Tg(CAG-EGFP) mice including brain tissue. With the dual-fluorescence tracing transplanted tumor model, we found that SU3 induced host cells malignant transformation in TME, and tumor/host cells fusion. In conclusion, EGFP is differentially and widely expressed in brain tissue of Foxn1nu .B6-Tg(CAG-EGFP), which is an ideal model for TME investigation. With Foxn1nu .B6-Tg(CAG-EGFP) mice, our research demonstrated that host cells malignant transformation and tumor/host cells fusion play an important role in tumor progression.
