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Christian Zwiener - One of the best experts on this subject based on the ideXlab platform.
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Combining in vitro reporter gene bioassays with Chemical analysis to assess changes in the water quality along the Ammer River, Southwestern Germany
Environmental Sciences Europe, 2018Co-Authors: Maximilian E. Müller, Beate I. Escher, Marc Schwientek, Martina Werneburg, Christiane Zarfl, Christian ZwienerAbstract:Background Rivers receive water and associated organic micropollutants from their entire catchment, including from urban, agricultural and natural sources, and constitute an important environmental component for catalyzing pollutant turnover. Environmental removal processes were extensively investigated under laboratory conditions in the past but there is still a lack of information on how organic micropollutants attenuate on the catchment scale. The aim of this study was to describe the Chemical and toxicological profile of a 4th order river and to characterize in-stream processes. We propose Indicator Chemicals and Indicator in vitro bioassays as screening methods to evaluate micropollutant input and transport and transformation processes of the Chemical burden in a river. Carbamazepine and sulfamethoxazole were selected as Indicators for dilution processes and the moderately degradable Chemicals tramadol and sotalol as Indicators for potential in-stream attenuation processes. The battery of bioassays covers seven environmentally relevant modes of action, namely estrogenicity, glucocorticogenic activity, androgenicity progestagenic activity and oxidative stress response, as well as activation of the peroxisome proliferator-activated receptor and the aryl hydrocarbon receptor, using the GeneBLAzer test battery and the AhR-CALUX and AREc32 assays. Results Both approaches, targeted Chemical analysis and in vitro bioassays, identified a wastewater treatment plant (WWTP) as a major input source of organic micropollutants that dominantly influenced the water quality of the river. Downstream of the WWTP the amount of detected Chemicals and biological effects decreased along the river flow. The organic Indicator Chemicals of known degradability uncovered dilution and potential loss processes in certain river stretches. The average cytotoxic potency of the river water decreased in a similar fashion as compounds of medium degradability such as the pharmaceutical sotalol. Conclusions This study showed that the Indicator Chemical/Indicator bioassay approach is suitable for identifying input sources of a mixture of organic micropollutants and to trace changes in the water quality along small rivers. This method forms the necessary basis for evaluating the natural attenuation processes of organic micropollutants on a catchment scale, especially when combined with enhanced sampling strategies in future studies.
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Combining in vitro reporter gene bioassays with Chemical analysis to assess changes in the water quality along the Ammer River, Southwestern Germany.
Environmental sciences Europe, 2018Co-Authors: Maximilian E. Müller, Beate I. Escher, Marc Schwientek, Martina Werneburg, Christiane Zarfl, Christian ZwienerAbstract:Rivers receive water and associated organic micropollutants from their entire catchment, including from urban, agricultural and natural sources, and constitute an important environmental component for catalyzing pollutant turnover. Environmental removal processes were extensively investigated under laboratory conditions in the past but there is still a lack of information on how organic micropollutants attenuate on the catchment scale. The aim of this study was to describe the Chemical and toxicological profile of a 4th order river and to characterize in-stream processes. We propose Indicator Chemicals and Indicator in vitro bioassays as screening methods to evaluate micropollutant input and transport and transformation processes of the Chemical burden in a river. Carbamazepine and sulfamethoxazole were selected as Indicators for dilution processes and the moderately degradable Chemicals tramadol and sotalol as Indicators for potential in-stream attenuation processes. The battery of bioassays covers seven environmentally relevant modes of action, namely estrogenicity, glucocorticogenic activity, androgenicity progestagenic activity and oxidative stress response, as well as activation of the peroxisome proliferator-activated receptor and the aryl hydrocarbon receptor, using the GeneBLAzer test battery and the AhR-CALUX and AREc32 assays. Both approaches, targeted Chemical analysis and in vitro bioassays, identified a wastewater treatment plant (WWTP) as a major input source of organic micropollutants that dominantly influenced the water quality of the river. Downstream of the WWTP the amount of detected Chemicals and biological effects decreased along the river flow. The organic Indicator Chemicals of known degradability uncovered dilution and potential loss processes in certain river stretches. The average cytotoxic potency of the river water decreased in a similar fashion as compounds of medium degradability such as the pharmaceutical sotalol. This study showed that the Indicator Chemical/Indicator bioassay approach is suitable for identifying input sources of a mixture of organic micropollutants and to trace changes in the water quality along small rivers. This method forms the necessary basis for evaluating the natural attenuation processes of organic micropollutants on a catchment scale, especially when combined with enhanced sampling strategies in future studies.
Maximilian E. Müller - One of the best experts on this subject based on the ideXlab platform.
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Combining in vitro reporter gene bioassays with Chemical analysis to assess changes in the water quality along the Ammer River, Southwestern Germany
Environmental Sciences Europe, 2018Co-Authors: Maximilian E. Müller, Beate I. Escher, Marc Schwientek, Martina Werneburg, Christiane Zarfl, Christian ZwienerAbstract:Background Rivers receive water and associated organic micropollutants from their entire catchment, including from urban, agricultural and natural sources, and constitute an important environmental component for catalyzing pollutant turnover. Environmental removal processes were extensively investigated under laboratory conditions in the past but there is still a lack of information on how organic micropollutants attenuate on the catchment scale. The aim of this study was to describe the Chemical and toxicological profile of a 4th order river and to characterize in-stream processes. We propose Indicator Chemicals and Indicator in vitro bioassays as screening methods to evaluate micropollutant input and transport and transformation processes of the Chemical burden in a river. Carbamazepine and sulfamethoxazole were selected as Indicators for dilution processes and the moderately degradable Chemicals tramadol and sotalol as Indicators for potential in-stream attenuation processes. The battery of bioassays covers seven environmentally relevant modes of action, namely estrogenicity, glucocorticogenic activity, androgenicity progestagenic activity and oxidative stress response, as well as activation of the peroxisome proliferator-activated receptor and the aryl hydrocarbon receptor, using the GeneBLAzer test battery and the AhR-CALUX and AREc32 assays. Results Both approaches, targeted Chemical analysis and in vitro bioassays, identified a wastewater treatment plant (WWTP) as a major input source of organic micropollutants that dominantly influenced the water quality of the river. Downstream of the WWTP the amount of detected Chemicals and biological effects decreased along the river flow. The organic Indicator Chemicals of known degradability uncovered dilution and potential loss processes in certain river stretches. The average cytotoxic potency of the river water decreased in a similar fashion as compounds of medium degradability such as the pharmaceutical sotalol. Conclusions This study showed that the Indicator Chemical/Indicator bioassay approach is suitable for identifying input sources of a mixture of organic micropollutants and to trace changes in the water quality along small rivers. This method forms the necessary basis for evaluating the natural attenuation processes of organic micropollutants on a catchment scale, especially when combined with enhanced sampling strategies in future studies.
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Combining in vitro reporter gene bioassays with Chemical analysis to assess changes in the water quality along the Ammer River, Southwestern Germany.
Environmental sciences Europe, 2018Co-Authors: Maximilian E. Müller, Beate I. Escher, Marc Schwientek, Martina Werneburg, Christiane Zarfl, Christian ZwienerAbstract:Rivers receive water and associated organic micropollutants from their entire catchment, including from urban, agricultural and natural sources, and constitute an important environmental component for catalyzing pollutant turnover. Environmental removal processes were extensively investigated under laboratory conditions in the past but there is still a lack of information on how organic micropollutants attenuate on the catchment scale. The aim of this study was to describe the Chemical and toxicological profile of a 4th order river and to characterize in-stream processes. We propose Indicator Chemicals and Indicator in vitro bioassays as screening methods to evaluate micropollutant input and transport and transformation processes of the Chemical burden in a river. Carbamazepine and sulfamethoxazole were selected as Indicators for dilution processes and the moderately degradable Chemicals tramadol and sotalol as Indicators for potential in-stream attenuation processes. The battery of bioassays covers seven environmentally relevant modes of action, namely estrogenicity, glucocorticogenic activity, androgenicity progestagenic activity and oxidative stress response, as well as activation of the peroxisome proliferator-activated receptor and the aryl hydrocarbon receptor, using the GeneBLAzer test battery and the AhR-CALUX and AREc32 assays. Both approaches, targeted Chemical analysis and in vitro bioassays, identified a wastewater treatment plant (WWTP) as a major input source of organic micropollutants that dominantly influenced the water quality of the river. Downstream of the WWTP the amount of detected Chemicals and biological effects decreased along the river flow. The organic Indicator Chemicals of known degradability uncovered dilution and potential loss processes in certain river stretches. The average cytotoxic potency of the river water decreased in a similar fashion as compounds of medium degradability such as the pharmaceutical sotalol. This study showed that the Indicator Chemical/Indicator bioassay approach is suitable for identifying input sources of a mixture of organic micropollutants and to trace changes in the water quality along small rivers. This method forms the necessary basis for evaluating the natural attenuation processes of organic micropollutants on a catchment scale, especially when combined with enhanced sampling strategies in future studies.
Randall B Pugh - One of the best experts on this subject based on the ideXlab platform.
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the use of chromophore and fluorophore degradation to quantitate uv dose fd c dyes as Chemical identicators for uv sterilization
Journal of Microbiological Methods, 2012Co-Authors: Karson S Putt, Edward R Kernick, Brieanna K Lohse, James Lomboy, Terry Obrien, Randall B PughAbstract:The accurate measurement of ultraviolet (UV) irradiation, especially within a container or vessel is one of the challenges facing the broad implementation of UV sterilization. Currently, biological Indicators are the best method to determine whether an applied UV dose has the necessary efficacy to achieve sterilization. To overcome some of the challenges of using a biological Indicator, Chemical Indicators based upon the degradation of food, drug and cosmetic (FD&C) dyes were developed. In this work, the relationship between UV dose and dye degradation was elucidated and used to create standard curves which could be used as a quantitative measurement system. The use of dye degradation as a measurement of UV dose is especially useful when the levels of UV irradiation within a container cannot be measured directly. Additionally, due to the highly colored nature of the FD&C dyes, the visual changes present upon dye irradiation can be used as a qualitative visual Indicator of the UV dose.
Escher Beate - One of the best experts on this subject based on the ideXlab platform.
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Application of cell-based bioassays to evaluate treatment efficacy of conventional and intensified treatment wetlands
'Royal Society of Chemistry (RSC)', 2018Co-Authors: Nivala Jaime, Neale, Peta A., Haasis Tobias, Kahl Stefanie, König Maria, Müller, Roland A., Reemtsma Thorsten, Schlichting Rita, Escher BeateAbstract:Constructed wetlands are commonly used for wastewater treatment when centralized sewage treatment is not feasible. Many studies have focused on the removal of micropollutants by treatment wetlands, but little is known about how well they can remove biological activity. Here we studied the removal efficacy of conventional and intensified treatment wetland designs using both Chemical analysis of conventional wastewater parameters and treatment Indicator Chemicals (caffeine, ibuprofen, naproxen, benzotriazole, diclofenac, acesulfame, carbamazepine) as well as a panel of in vitro bioassays indicative of different stages of cellular toxicity pathways, such as xenobiotic metabolism, receptor-mediated effect and adaptive stress responses. Water samples collected before and after seven treatment wetlands were compared against the adjacent municipal wastewater treatment plant. The intensified treatment wetlands generally removed micropollutants and biological activity to a greater extent than the conventional wastewater treatment plant, whereas the conventional horizontal subsurface flow wetland showed poor removal of all Indicators. Carbamazepine was not well removed by any of the studied systems as expected from reported recalcitrance in aerobic environments. Estrogenic activity, which is a commonly used biological endpoint Indicator for wastewater treatment, was removed very well by the intensified wetlands (97 to 99.5%) with similar or slightly lower removal efficacy for all other biological endpoints. The results highlight the importance of applying Indicator bioassays complementary to Indicator Chemical analysis for monitoring treatment efficacy. The high removal efficacy of biological effects as a measure of total effect-scaled concentrations of Chemicals provides further support to the use of intensified wetlands for wastewater treatment
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Application of cell-based bioassays to evaluate treatment efficacy of conventional and intensified treatment wetlands
Royal Society of Chemistry, 2018Co-Authors: Nivala Jaime, Haasis Tobias, Kahl Stefanie, König Maria, Reemtsma Thorsten, Schlichting Rita, Neale Peta, Muller Roland, Escher BeateAbstract:Constructed wetlands are commonly used for wastewater treatment when centralized sewage treatment is not feasible. Many studies have focused on the removal of micropollutants by treatment wetlands, but little is known about how well they can remove biological activity. Here we studied the removal efficacy of conventional and intensified treatment wetland designs using both Chemical analysis of conventional wastewater parameters and treatment Indicator Chemicals (caffeine, ibuprofen, naproxen, benzotriazole, diclofenac, acesulfame, carbamazepine) as well as a panel of in vitro bioassays indicative of different stages of cellular toxicity pathways, such as xenobiotic metabolism, receptor-mediated effect and adaptive stress responses. Water samples collected before and after seven treatment wetlands were compared against the adjacent municipal wastewater treatment plant. The intensified treatment wetlands generally removed micropollutants and biological activity to a greater extent than the conventional wastewater treatment plant, whereas the conventional horizontal subsurface flow wetland showed poor removal of all Indicators. Carbamazepine was not well removed by any of the studied systems as expected from reported recalcitrance in aerobic environments. Estrogenic activity, which is a commonly used biological endpoint Indicator for wastewater treatment, was removed very well by the intensified wetlands (97 to 99.5%) with similar or slightly lower removal efficacy for all other biological endpoints. The results highlight the importance of applying Indicator bioassays complementary to Indicator Chemical analysis for monitoring treatment efficacy. The high removal efficacy of biological effects as a measure of total effect-scaled concentrations of Chemicals provides further support to the use of intensified wetlands for wastewater treatment.Full Tex
Martina Werneburg - One of the best experts on this subject based on the ideXlab platform.
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Combining in vitro reporter gene bioassays with Chemical analysis to assess changes in the water quality along the Ammer River, Southwestern Germany
Environmental Sciences Europe, 2018Co-Authors: Maximilian E. Müller, Beate I. Escher, Marc Schwientek, Martina Werneburg, Christiane Zarfl, Christian ZwienerAbstract:Background Rivers receive water and associated organic micropollutants from their entire catchment, including from urban, agricultural and natural sources, and constitute an important environmental component for catalyzing pollutant turnover. Environmental removal processes were extensively investigated under laboratory conditions in the past but there is still a lack of information on how organic micropollutants attenuate on the catchment scale. The aim of this study was to describe the Chemical and toxicological profile of a 4th order river and to characterize in-stream processes. We propose Indicator Chemicals and Indicator in vitro bioassays as screening methods to evaluate micropollutant input and transport and transformation processes of the Chemical burden in a river. Carbamazepine and sulfamethoxazole were selected as Indicators for dilution processes and the moderately degradable Chemicals tramadol and sotalol as Indicators for potential in-stream attenuation processes. The battery of bioassays covers seven environmentally relevant modes of action, namely estrogenicity, glucocorticogenic activity, androgenicity progestagenic activity and oxidative stress response, as well as activation of the peroxisome proliferator-activated receptor and the aryl hydrocarbon receptor, using the GeneBLAzer test battery and the AhR-CALUX and AREc32 assays. Results Both approaches, targeted Chemical analysis and in vitro bioassays, identified a wastewater treatment plant (WWTP) as a major input source of organic micropollutants that dominantly influenced the water quality of the river. Downstream of the WWTP the amount of detected Chemicals and biological effects decreased along the river flow. The organic Indicator Chemicals of known degradability uncovered dilution and potential loss processes in certain river stretches. The average cytotoxic potency of the river water decreased in a similar fashion as compounds of medium degradability such as the pharmaceutical sotalol. Conclusions This study showed that the Indicator Chemical/Indicator bioassay approach is suitable for identifying input sources of a mixture of organic micropollutants and to trace changes in the water quality along small rivers. This method forms the necessary basis for evaluating the natural attenuation processes of organic micropollutants on a catchment scale, especially when combined with enhanced sampling strategies in future studies.
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Combining in vitro reporter gene bioassays with Chemical analysis to assess changes in the water quality along the Ammer River, Southwestern Germany.
Environmental sciences Europe, 2018Co-Authors: Maximilian E. Müller, Beate I. Escher, Marc Schwientek, Martina Werneburg, Christiane Zarfl, Christian ZwienerAbstract:Rivers receive water and associated organic micropollutants from their entire catchment, including from urban, agricultural and natural sources, and constitute an important environmental component for catalyzing pollutant turnover. Environmental removal processes were extensively investigated under laboratory conditions in the past but there is still a lack of information on how organic micropollutants attenuate on the catchment scale. The aim of this study was to describe the Chemical and toxicological profile of a 4th order river and to characterize in-stream processes. We propose Indicator Chemicals and Indicator in vitro bioassays as screening methods to evaluate micropollutant input and transport and transformation processes of the Chemical burden in a river. Carbamazepine and sulfamethoxazole were selected as Indicators for dilution processes and the moderately degradable Chemicals tramadol and sotalol as Indicators for potential in-stream attenuation processes. The battery of bioassays covers seven environmentally relevant modes of action, namely estrogenicity, glucocorticogenic activity, androgenicity progestagenic activity and oxidative stress response, as well as activation of the peroxisome proliferator-activated receptor and the aryl hydrocarbon receptor, using the GeneBLAzer test battery and the AhR-CALUX and AREc32 assays. Both approaches, targeted Chemical analysis and in vitro bioassays, identified a wastewater treatment plant (WWTP) as a major input source of organic micropollutants that dominantly influenced the water quality of the river. Downstream of the WWTP the amount of detected Chemicals and biological effects decreased along the river flow. The organic Indicator Chemicals of known degradability uncovered dilution and potential loss processes in certain river stretches. The average cytotoxic potency of the river water decreased in a similar fashion as compounds of medium degradability such as the pharmaceutical sotalol. This study showed that the Indicator Chemical/Indicator bioassay approach is suitable for identifying input sources of a mixture of organic micropollutants and to trace changes in the water quality along small rivers. This method forms the necessary basis for evaluating the natural attenuation processes of organic micropollutants on a catchment scale, especially when combined with enhanced sampling strategies in future studies.
