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Isao Yumoto - One of the best experts on this subject based on the ideXlab platform.
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fermentibacillus polygoni gen nov sp nov an alkaliphile that reduces Indigo Dye
International Journal of Systematic and Evolutionary Microbiology, 2016Co-Authors: Kikue Hirota, Isao Yumoto, Kenichi AinoAbstract:Facultatively alkaliphilic strains, designated as strains IEB3T and IEB14, were isolated as Indigo-reducing strains from a fermented Polygonum Indigo (Polygonum tinctorium Lour) liquor sample prepared in our laboratory using a medium containing an Indigo fermentation liquor as a sole substrate. The 16S rRNA gene sequence phylogeny and similarity suggested that strains IEB3T and IEB14 exhibit distinctive positions among the members of the genus Bacillus, and their closest neighbour was Bacillus nanhaiisediminis NH3T (similarity: 97.4 %) among the species with validly published names. The 16S rRNA sequence of strain IEB3Twas identical to that of strain IEB14. The cells of the isolates stained Gram-positive and were facultatively anaerobic, straight rods that were motile by a pair of subpolar flagella. Strains IEB3T and IEB14 grew at temperatures between 12 and 40 °C with optimum growth at 30‒33 °C and in the range of pH 7.5-12. Menaquinone-7 (MK-7) was detected as the major isoprenoid quinone. The DNA G+C contents of strains IEB3T and IEB14 were 39.0 and 39.1 mol%, respectively. The whole-cell fatty acid profile mainly (>10 %) consisted of iso-C14:0, iso-C15:0 and anteiso-C15:0. DNA-DNA hybridization revealed a low relatedness value between strain IEB3T and the phylogenetically most closely related species, Bacillus nanhaiisediminis JCM 16507T (<7 % ). On the basis of phenotypic and chemotaxonomic characteristics and phylogenetic data, the isolates represent a novel species within a novel genus, for which the name Fermentibacillus polygoni gen. nov., sp. nov. is proposed. The type strain is IEB3T (=JCM 30817T=NCIMB 14984T).
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amphibacillus iburiensis sp nov an alkaliphile that reduces an Indigo Dye
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Kikue Hirota, Isao Yumoto, Kenichi AinoAbstract:An Indigo-reducing alkaliphilic strain, designated strain N314T, was isolated from a fermented polygonum Indigo (Polygonum tinctorium Lour.) liquor sample, aged for 10 months, that was obtained from Date City, Iburi Branch, Hokkaido, Japan. The 16S rRNA gene sequence phylogeny suggested that strain N314T is a member of the genus Amphibacillus , with the closest relatives being Amphibacillus indicireducens (98.9 % similarity to the type strain) and Amphibacillus xylanus (98.0 % similarity to the type strain), the only species with 16S rRNA gene sequence similarities higher than 97 % to strain N314T. The cells of the isolate stained Gram-positive and were facultatively anaerobic, straight rods that were motile by means of peritrichous flagella. The strain grew at 26–39 °C with optimum growth at 36 °C. It grew at pH 8.0–9.1, with optimum growth at pH 8.9–9.1. No isoprenoid quinone was detected, and the DNA G+C content was 38.4 mol%. The whole-cell fatty acid profile consisted mainly of iso-C15 : 0 and anteiso-C15 : 0. Analysis of DNA–DNA hybridization with the type strains of A. indicireducens and A. xylanus revealed 29±2 % and 10±2 % relatedness, respectively. Owing to differences in phenotypic characteristics from reported species of the genus A. and results of phylogenetic analyses based on 16S rRNA gene sequences and DNA–DNA relatedness data, the isolate merits classification within a novel species, for which the name Amphibacillus iburiensis sp. nov. is proposed. The type strain is N314T ( = JCM 18529T = NCIMB 14823T).
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oceanobacillus indicireducens sp nov a facultative alkaliphile that reduces an Indigo Dye
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Kikue Hirota, Isao Yumoto, Kenichi Aino, Yoshinobu NodasakaAbstract:An Indigo-reducing facultatively alkaliphilic and halophilic strain, designated strain A21T, was isolated from a fermented Polygonum Indigo (Polygonum tinctorium Lour.) liquor sample aged for 4 days prepared in a laboratory. 16S rRNA gene sequence phylogeny suggested that strain A21T was a member of the genus Oceanobacillus with the closest relative being the type strain of Oceanobacillus chironomi (similarity: 96.0 %). The cells of the isolate stained Gram-positive and were facultatively anaerobic straight rods that were motile by peritrichous flagella. The strain grew between 18 and 48 °C with optimum growth at 39 °C. It grew in the pH range of 7–12. It hydrolysed casein, gelatin and Tween 20 but not Tweens 40, 60 and 80, starch or DNA. No isoprenoid quinone was detected and the DNA G+C content was 39.7 mol%. The whole-cell fatty acid profile mainly consisted of iso-C15 : 0, anteiso-C15 : 0 and C16 : 0. DNA–DNA hybridization experiments with O. chironomi revealed 13 % relatedness. Owing to the differences in phenotypic and chemotaxonomic characteristics, and phylogenetic analyses based on 16S rRNA gene sequences and DNA–DNA relatedness data from reported Oceanobacillus species, the isolate merits classification as a representative of a novel species, for which the name Oceanobacillus indicireducens sp. nov. is proposed. The type strain is A21T ( = JCM 17251T = NCIMB 14685T). The description of the genus Oceanobacillus is also emended.
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amphibacillus indicireducens sp nov an alkaliphile that reduces an Indigo Dye
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Kikue Hirota, Isao Yumoto, Kenichi Aino, Naoki Morita, Yoshinobu NodasakaAbstract:Two Indigo-reducing alkaliphilic strains, designated strain C40T and strain N214, were isolated from a fermented Polygonum Indigo (Polygonum tinctorium Lour.) liquor sample aged for 10 months and obtained from Date City, Hokkaido, Japan. 16S rRNA gene sequence phylogeny suggested that strains C40T and N214 were members of the genus Amphibacillus with the closest relative being Amphibacillus xylanus JCM 7361T (97.5 % 16S rRNA gene sequence similarity with strain C40T), which is the only strain having a 16S rRNA gene sequence similarity higher than 97 % with strain C40T. Cells of strain C40T were Gram-stain-positive, facultatively anaerobic, straight rods that were motile by means of peritrichous flagella. The strains grew between 17 and 39 °C (optimum, 35 °C) and in the pH range of 9.0–12.0. No isoprenoid quinone was detected and the DNA G+C content was 37.5–37.7 mol%. The whole-cell fatty acid profile mainly consisted of iso-C15 : 0 and anteiso-C15 : 0. DNA–DNA hybridization of strain C40T with Amphibacillus xylanus JCM 7361T revealed a DNA–DNA relatedness value of 10±3 %. Owing to the differences in phenotypic characteristics and phylogenetic analyses based on 16S rRNA gene sequences, as well as DNA–DNA relatedness data from reported species of the genus Amphibacillus , the isolates merit classification as a novel species in the genus Amphibacillus , for which the name Amphibacillus indicireducens sp. nov. is proposed. The type strain is C40T ( = JCM 17250T = NCIMB 14686T). An additional strain of the species is N214. An emended description of the genus Amphibacillus is provided.
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alkalibacterium indicireducens sp nov an obligate alkaliphile that reduces Indigo Dye
International Journal of Systematic and Evolutionary Microbiology, 2008Co-Authors: Isao Yumoto, Kikue Hirota, Yoshinobu Nodasaka, Yutaka Tokiwa, Kenji NakajimaAbstract:Indigo-reducing, obligately alkaliphilic strains A11T, F11 and F12 were isolated from Indigo fermentation liquor obtained from Tokushima Prefecture, Shikoku, Japan. The isolates grew at pH 9.0-12.3, but not at pH 7.0-8.0. The optimum pH range for growth was 9.5-11.5. They were Gram-negative, facultatively anaerobic, rod-shaped strains with peritrichous flagella. The isolates grew in 0-14 % (w/v) NaCl, with optimum growth at 1-11 %. They grew at temperatures of 15-35 degrees C with optimum growth at around 20-30 degrees C. dl-Lactate was the major end product from d-glucose. No quinones were detected. The peptidoglycan type was A4 alpha, l-Lys (l-Orn)-d-Asp. The major cellular fatty acids were C16 : 0, C16 : 17c and C18 : 19c. The DNA G+C contents were 47.0-47.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequence data indicated that the isolates belong to the genus Alkalibacterium. DNA-DNA hybridization revealed low relatedness values between the isolates and the three phylogenetically most closely related species, Alkalibacterium olivapovliticus, Alkalibacterium psychrotolerans and Alkalibacterium iburiense (<41 %). On the basis of phenotypic characteristics, including hydrolysis of cellulose and fermentation of carbohydrates, and chemotaxonomic characteristics, phylogenetic data and DNA-DNA relatedness data, it is concluded that the isolates merit classification as representatives of a novel species of the genus Alkalibacterium, for which the name Alkalibacterium indicireducens sp. nov. is proposed. The type strain of this species is A11T (=JCM 14232T=NCIMB 14253T).
Anthony B Cunningham - One of the best experts on this subject based on the ideXlab platform.
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identity blues the ethnobotany of the Indigo Dyeing by landian yao iu mien in yunnan southwest china
Journal of Ethnobiology and Ethnomedicine, 2019Co-Authors: Anthony B Cunningham, Ruyan Fan, Yuhua WangAbstract:Indigo-Dyed textiles have been central to the cultural identity of Landian Yao (literally “blue clothes Yao”) people in Southwest China for centuries, driving a significant local market for naturally Dyed Indigo cloth. In the past two decades, local Indigo production for traditional textiles has declined for several reasons: Firstly, the younger generation of Landian Yao has shifted to using western style jeans and T-shirts. Secondly, due to its labor-intensive nature. In contrast, at a global scale, including in China, there has been a revival of interest in natural Indigo use. This is due to a growing awareness in the fashion industry about human and environmental health issues related to synthetic Dye production. Ironically, this new awareness comes at a time when traditional knowledge of Indigo Dyeing is being lost in many places in China, with weaving and use of natural Dyes now limited to some remote areas. In this study, we recorded Indigo Dyeing processes used by Landian Yao people and documented the plant species used for Indigo Dyeing. Field surveys were conducted to the study area from September 2015 to November 2016, supplemented by follow-up visits in July 2018 and November 2018. We interviewed 46 key informants between 36 and 82 years old who still continued traditional Indigo Dyeing practices. Most were elderly people. Semi-structured interviews were used. During the field study, we kept a detailed account of the methods used by Landian Yao Dyers. The data were then analyzed by using utilization frequency to determine the best traditional recipe of Indigo Dye extraction. All the specimens of documented species were collected and deposited at the herbarium of Kunming Institute of Botany. Our results showed that Indigo Dyeing was divided into two main steps: (1) Indigo pigment extraction and (2) Dyeing cloth. The general procedures of Indigo Dye extraction included building or buying a Dye vat, fermentation, removal of the leaves of Indigo producing plant species, addition of lime, oxygenation, followed by collection, and the storage of the Indigo paste. The procedures of Dyeing cloth included preparing the Dye solutions, Dyeing cloth, washing, and air drying. It is notable that Landian Yao Dyers formerly only performed the Dyeing process on the goat days in the lunar calendar from June to October. After comparing the range of local Indigo extraction methods, our results showed that the following was best of these traditional recipes: a Indigo-yielding plant material to tap water ratio of 30 kg: 200 l, lime 3 kg, a fermentation time of 2–3 d, aeration by agitation for up to 60 min, and a precipitation time of 2–3 h. Our results show that 17 plant species in 11 families were recorded in the Indigo Dyeing process. With the exception of the Indigo sources, only Dioscorea cirrhosa Lour. and Artemisia argyi H.Lev. & Vaniot were previously recorded in Dyeing processes. Other species given in this paper are recorded for the first time in terms of their use in the Indigo Dyeing process. In the study area, Landian Yao men were in charge of Indigo Dye extraction, and the women were responsible for Dyeing cloth. The Landian Yao has completely mastered the traditional Indigo Dyeing craft and are one of the well-deserved identity blues. Indigo production from plants using traditional methods is a slow process compared to synthetic Dyes and is not suitable for modern and rapid industrial production. Therefore, our study records the detailed information of traditional Indigo Dyeing to protect and inherit it. Strobilanthes cusia (Nees) Kuntze is the main Indigo source in Landian Yao that is widely used in the world and can be commercially exploited as an Indigo plant. For commercial and environment benefits, we suggest that producing natural Indigo for the commercial market is a good choice.
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Identity blues: the ethnobotany of the Indigo Dyeing by Landian Yao (Iu Mien) in Yunnan, Southwest China
BMC, 2019Co-Authors: Anthony B Cunningham, Ruyan Fan, Yuhua WangAbstract:Abstract Background Indigo-Dyed textiles have been central to the cultural identity of Landian Yao (literally “blue clothes Yao”) people in Southwest China for centuries, driving a significant local market for naturally Dyed Indigo cloth. In the past two decades, local Indigo production for traditional textiles has declined for several reasons: Firstly, the younger generation of Landian Yao has shifted to using western style jeans and T-shirts. Secondly, due to its labor-intensive nature. In contrast, at a global scale, including in China, there has been a revival of interest in natural Indigo use. This is due to a growing awareness in the fashion industry about human and environmental health issues related to synthetic Dye production. Ironically, this new awareness comes at a time when traditional knowledge of Indigo Dyeing is being lost in many places in China, with weaving and use of natural Dyes now limited to some remote areas. In this study, we recorded Indigo Dyeing processes used by Landian Yao people and documented the plant species used for Indigo Dyeing. Methods Field surveys were conducted to the study area from September 2015 to November 2016, supplemented by follow-up visits in July 2018 and November 2018. We interviewed 46 key informants between 36 and 82 years old who still continued traditional Indigo Dyeing practices. Most were elderly people. Semi-structured interviews were used. During the field study, we kept a detailed account of the methods used by Landian Yao Dyers. The data were then analyzed by using utilization frequency to determine the best traditional recipe of Indigo Dye extraction. All the specimens of documented species were collected and deposited at the herbarium of Kunming Institute of Botany. Results Our results showed that Indigo Dyeing was divided into two main steps: (1) Indigo pigment extraction and (2) Dyeing cloth. The general procedures of Indigo Dye extraction included building or buying a Dye vat, fermentation, removal of the leaves of Indigo producing plant species, addition of lime, oxygenation, followed by collection, and the storage of the Indigo paste. The procedures of Dyeing cloth included preparing the Dye solutions, Dyeing cloth, washing, and air drying. It is notable that Landian Yao Dyers formerly only performed the Dyeing process on the goat days in the lunar calendar from June to October. After comparing the range of local Indigo extraction methods, our results showed that the following was best of these traditional recipes: a Indigo-yielding plant material to tap water ratio of 30 kg: 200 l, lime 3 kg, a fermentation time of 2–3 d, aeration by agitation for up to 60 min, and a precipitation time of 2–3 h. Our results show that 17 plant species in 11 families were recorded in the Indigo Dyeing process. With the exception of the Indigo sources, only Dioscorea cirrhosa Lour. and Artemisia argyi H.Lév. & Vaniot were previously recorded in Dyeing processes. Other species given in this paper are recorded for the first time in terms of their use in the Indigo Dyeing process. In the study area, Landian Yao men were in charge of Indigo Dye extraction, and the women were responsible for Dyeing cloth. Conclusions The Landian Yao has completely mastered the traditional Indigo Dyeing craft and are one of the well-deserved identity blues. Indigo production from plants using traditional methods is a slow process compared to synthetic Dyes and is not suitable for modern and rapid industrial production. Therefore, our study records the detailed information of traditional Indigo Dyeing to protect and inherit it. Strobilanthes cusia (Nees) Kuntze is the main Indigo source in Landian Yao that is widely used in the world and can be commercially exploited as an Indigo plant. For commercial and environment benefits, we suggest that producing natural Indigo for the commercial market is a good choice
Kikue Hirota - One of the best experts on this subject based on the ideXlab platform.
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fermentibacillus polygoni gen nov sp nov an alkaliphile that reduces Indigo Dye
International Journal of Systematic and Evolutionary Microbiology, 2016Co-Authors: Kikue Hirota, Isao Yumoto, Kenichi AinoAbstract:Facultatively alkaliphilic strains, designated as strains IEB3T and IEB14, were isolated as Indigo-reducing strains from a fermented Polygonum Indigo (Polygonum tinctorium Lour) liquor sample prepared in our laboratory using a medium containing an Indigo fermentation liquor as a sole substrate. The 16S rRNA gene sequence phylogeny and similarity suggested that strains IEB3T and IEB14 exhibit distinctive positions among the members of the genus Bacillus, and their closest neighbour was Bacillus nanhaiisediminis NH3T (similarity: 97.4 %) among the species with validly published names. The 16S rRNA sequence of strain IEB3Twas identical to that of strain IEB14. The cells of the isolates stained Gram-positive and were facultatively anaerobic, straight rods that were motile by a pair of subpolar flagella. Strains IEB3T and IEB14 grew at temperatures between 12 and 40 °C with optimum growth at 30‒33 °C and in the range of pH 7.5-12. Menaquinone-7 (MK-7) was detected as the major isoprenoid quinone. The DNA G+C contents of strains IEB3T and IEB14 were 39.0 and 39.1 mol%, respectively. The whole-cell fatty acid profile mainly (>10 %) consisted of iso-C14:0, iso-C15:0 and anteiso-C15:0. DNA-DNA hybridization revealed a low relatedness value between strain IEB3T and the phylogenetically most closely related species, Bacillus nanhaiisediminis JCM 16507T (<7 % ). On the basis of phenotypic and chemotaxonomic characteristics and phylogenetic data, the isolates represent a novel species within a novel genus, for which the name Fermentibacillus polygoni gen. nov., sp. nov. is proposed. The type strain is IEB3T (=JCM 30817T=NCIMB 14984T).
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amphibacillus iburiensis sp nov an alkaliphile that reduces an Indigo Dye
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Kikue Hirota, Isao Yumoto, Kenichi AinoAbstract:An Indigo-reducing alkaliphilic strain, designated strain N314T, was isolated from a fermented polygonum Indigo (Polygonum tinctorium Lour.) liquor sample, aged for 10 months, that was obtained from Date City, Iburi Branch, Hokkaido, Japan. The 16S rRNA gene sequence phylogeny suggested that strain N314T is a member of the genus Amphibacillus , with the closest relatives being Amphibacillus indicireducens (98.9 % similarity to the type strain) and Amphibacillus xylanus (98.0 % similarity to the type strain), the only species with 16S rRNA gene sequence similarities higher than 97 % to strain N314T. The cells of the isolate stained Gram-positive and were facultatively anaerobic, straight rods that were motile by means of peritrichous flagella. The strain grew at 26–39 °C with optimum growth at 36 °C. It grew at pH 8.0–9.1, with optimum growth at pH 8.9–9.1. No isoprenoid quinone was detected, and the DNA G+C content was 38.4 mol%. The whole-cell fatty acid profile consisted mainly of iso-C15 : 0 and anteiso-C15 : 0. Analysis of DNA–DNA hybridization with the type strains of A. indicireducens and A. xylanus revealed 29±2 % and 10±2 % relatedness, respectively. Owing to differences in phenotypic characteristics from reported species of the genus A. and results of phylogenetic analyses based on 16S rRNA gene sequences and DNA–DNA relatedness data, the isolate merits classification within a novel species, for which the name Amphibacillus iburiensis sp. nov. is proposed. The type strain is N314T ( = JCM 18529T = NCIMB 14823T).
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oceanobacillus indicireducens sp nov a facultative alkaliphile that reduces an Indigo Dye
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Kikue Hirota, Isao Yumoto, Kenichi Aino, Yoshinobu NodasakaAbstract:An Indigo-reducing facultatively alkaliphilic and halophilic strain, designated strain A21T, was isolated from a fermented Polygonum Indigo (Polygonum tinctorium Lour.) liquor sample aged for 4 days prepared in a laboratory. 16S rRNA gene sequence phylogeny suggested that strain A21T was a member of the genus Oceanobacillus with the closest relative being the type strain of Oceanobacillus chironomi (similarity: 96.0 %). The cells of the isolate stained Gram-positive and were facultatively anaerobic straight rods that were motile by peritrichous flagella. The strain grew between 18 and 48 °C with optimum growth at 39 °C. It grew in the pH range of 7–12. It hydrolysed casein, gelatin and Tween 20 but not Tweens 40, 60 and 80, starch or DNA. No isoprenoid quinone was detected and the DNA G+C content was 39.7 mol%. The whole-cell fatty acid profile mainly consisted of iso-C15 : 0, anteiso-C15 : 0 and C16 : 0. DNA–DNA hybridization experiments with O. chironomi revealed 13 % relatedness. Owing to the differences in phenotypic and chemotaxonomic characteristics, and phylogenetic analyses based on 16S rRNA gene sequences and DNA–DNA relatedness data from reported Oceanobacillus species, the isolate merits classification as a representative of a novel species, for which the name Oceanobacillus indicireducens sp. nov. is proposed. The type strain is A21T ( = JCM 17251T = NCIMB 14685T). The description of the genus Oceanobacillus is also emended.
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amphibacillus indicireducens sp nov an alkaliphile that reduces an Indigo Dye
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Kikue Hirota, Isao Yumoto, Kenichi Aino, Naoki Morita, Yoshinobu NodasakaAbstract:Two Indigo-reducing alkaliphilic strains, designated strain C40T and strain N214, were isolated from a fermented Polygonum Indigo (Polygonum tinctorium Lour.) liquor sample aged for 10 months and obtained from Date City, Hokkaido, Japan. 16S rRNA gene sequence phylogeny suggested that strains C40T and N214 were members of the genus Amphibacillus with the closest relative being Amphibacillus xylanus JCM 7361T (97.5 % 16S rRNA gene sequence similarity with strain C40T), which is the only strain having a 16S rRNA gene sequence similarity higher than 97 % with strain C40T. Cells of strain C40T were Gram-stain-positive, facultatively anaerobic, straight rods that were motile by means of peritrichous flagella. The strains grew between 17 and 39 °C (optimum, 35 °C) and in the pH range of 9.0–12.0. No isoprenoid quinone was detected and the DNA G+C content was 37.5–37.7 mol%. The whole-cell fatty acid profile mainly consisted of iso-C15 : 0 and anteiso-C15 : 0. DNA–DNA hybridization of strain C40T with Amphibacillus xylanus JCM 7361T revealed a DNA–DNA relatedness value of 10±3 %. Owing to the differences in phenotypic characteristics and phylogenetic analyses based on 16S rRNA gene sequences, as well as DNA–DNA relatedness data from reported species of the genus Amphibacillus , the isolates merit classification as a novel species in the genus Amphibacillus , for which the name Amphibacillus indicireducens sp. nov. is proposed. The type strain is C40T ( = JCM 17250T = NCIMB 14686T). An additional strain of the species is N214. An emended description of the genus Amphibacillus is provided.
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alkalibacterium indicireducens sp nov an obligate alkaliphile that reduces Indigo Dye
International Journal of Systematic and Evolutionary Microbiology, 2008Co-Authors: Isao Yumoto, Kikue Hirota, Yoshinobu Nodasaka, Yutaka Tokiwa, Kenji NakajimaAbstract:Indigo-reducing, obligately alkaliphilic strains A11T, F11 and F12 were isolated from Indigo fermentation liquor obtained from Tokushima Prefecture, Shikoku, Japan. The isolates grew at pH 9.0-12.3, but not at pH 7.0-8.0. The optimum pH range for growth was 9.5-11.5. They were Gram-negative, facultatively anaerobic, rod-shaped strains with peritrichous flagella. The isolates grew in 0-14 % (w/v) NaCl, with optimum growth at 1-11 %. They grew at temperatures of 15-35 degrees C with optimum growth at around 20-30 degrees C. dl-Lactate was the major end product from d-glucose. No quinones were detected. The peptidoglycan type was A4 alpha, l-Lys (l-Orn)-d-Asp. The major cellular fatty acids were C16 : 0, C16 : 17c and C18 : 19c. The DNA G+C contents were 47.0-47.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequence data indicated that the isolates belong to the genus Alkalibacterium. DNA-DNA hybridization revealed low relatedness values between the isolates and the three phylogenetically most closely related species, Alkalibacterium olivapovliticus, Alkalibacterium psychrotolerans and Alkalibacterium iburiense (<41 %). On the basis of phenotypic characteristics, including hydrolysis of cellulose and fermentation of carbohydrates, and chemotaxonomic characteristics, phylogenetic data and DNA-DNA relatedness data, it is concluded that the isolates merit classification as representatives of a novel species of the genus Alkalibacterium, for which the name Alkalibacterium indicireducens sp. nov. is proposed. The type strain of this species is A11T (=JCM 14232T=NCIMB 14253T).
Yuhua Wang - One of the best experts on this subject based on the ideXlab platform.
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Blue footprint: Distribution and use of Indigo-yielding plant species Strobilanthes cusia (Nees) Kuntze
'Elsevier BV', 2021Co-Authors: Libin Zhang, Huizhao Yang, Yanan Wang, Huifu Zhuang, Wenyun Chen, Zihong Lin, Yuhua WangAbstract:Background: Strobilanthes cusia (Nees) Kuntze is an important medicinal, edible, and Indigo-yielding plant species. It has been cultivated for centuries and it is often the only natural Dye still used in many places of East and Southeast Asia. Although S. cisia is extensively cultivated and widely used, the ecological factors of its environmental demands are poorly understood. Moreover, with the increasing demand and growing habitat degradation, its wild populations are in sharp decline. It is therefore imperative to understand the socio-ecological interactions of this species regarding its climatic niche, ethnobotanical importance, and human relations in order to meet its demand. Methods: We first collected S. cusia occurrences from plant species databases, literatures and ethnobotanical surveys. 244 wild occurrences and 10 variables were used to predict its suitable habitats using the MaxEnt model. Furthermore, for a better understanding of socio-ecological interactions regarding its distribution and use, we also collected use reports and calculated the relative importance level (RIL). Results: Strobilanthes cusia is valued for many reasons, as it contains numerous health benefits as a medicinal plant and use in tea alongside its Indigo dying and tattooing properties. Indigo Dye, Southern Banlangen, and Indigo Naturalis are its most important usages. Its suitable habitats are chiefly located in Western and Central Himalayas, Southern China, and Southern Japan. The ‘temperature seasonality (standard deviation × 100)’ (bio4), the ‘precipitation of coldest quarter’ (bio19), and the human footprint index showed the strongest association with the relative contributions of 38.5%, 32.4%, and 9.1%, respectively
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identity blues the ethnobotany of the Indigo Dyeing by landian yao iu mien in yunnan southwest china
Journal of Ethnobiology and Ethnomedicine, 2019Co-Authors: Anthony B Cunningham, Ruyan Fan, Yuhua WangAbstract:Indigo-Dyed textiles have been central to the cultural identity of Landian Yao (literally “blue clothes Yao”) people in Southwest China for centuries, driving a significant local market for naturally Dyed Indigo cloth. In the past two decades, local Indigo production for traditional textiles has declined for several reasons: Firstly, the younger generation of Landian Yao has shifted to using western style jeans and T-shirts. Secondly, due to its labor-intensive nature. In contrast, at a global scale, including in China, there has been a revival of interest in natural Indigo use. This is due to a growing awareness in the fashion industry about human and environmental health issues related to synthetic Dye production. Ironically, this new awareness comes at a time when traditional knowledge of Indigo Dyeing is being lost in many places in China, with weaving and use of natural Dyes now limited to some remote areas. In this study, we recorded Indigo Dyeing processes used by Landian Yao people and documented the plant species used for Indigo Dyeing. Field surveys were conducted to the study area from September 2015 to November 2016, supplemented by follow-up visits in July 2018 and November 2018. We interviewed 46 key informants between 36 and 82 years old who still continued traditional Indigo Dyeing practices. Most were elderly people. Semi-structured interviews were used. During the field study, we kept a detailed account of the methods used by Landian Yao Dyers. The data were then analyzed by using utilization frequency to determine the best traditional recipe of Indigo Dye extraction. All the specimens of documented species were collected and deposited at the herbarium of Kunming Institute of Botany. Our results showed that Indigo Dyeing was divided into two main steps: (1) Indigo pigment extraction and (2) Dyeing cloth. The general procedures of Indigo Dye extraction included building or buying a Dye vat, fermentation, removal of the leaves of Indigo producing plant species, addition of lime, oxygenation, followed by collection, and the storage of the Indigo paste. The procedures of Dyeing cloth included preparing the Dye solutions, Dyeing cloth, washing, and air drying. It is notable that Landian Yao Dyers formerly only performed the Dyeing process on the goat days in the lunar calendar from June to October. After comparing the range of local Indigo extraction methods, our results showed that the following was best of these traditional recipes: a Indigo-yielding plant material to tap water ratio of 30 kg: 200 l, lime 3 kg, a fermentation time of 2–3 d, aeration by agitation for up to 60 min, and a precipitation time of 2–3 h. Our results show that 17 plant species in 11 families were recorded in the Indigo Dyeing process. With the exception of the Indigo sources, only Dioscorea cirrhosa Lour. and Artemisia argyi H.Lev. & Vaniot were previously recorded in Dyeing processes. Other species given in this paper are recorded for the first time in terms of their use in the Indigo Dyeing process. In the study area, Landian Yao men were in charge of Indigo Dye extraction, and the women were responsible for Dyeing cloth. The Landian Yao has completely mastered the traditional Indigo Dyeing craft and are one of the well-deserved identity blues. Indigo production from plants using traditional methods is a slow process compared to synthetic Dyes and is not suitable for modern and rapid industrial production. Therefore, our study records the detailed information of traditional Indigo Dyeing to protect and inherit it. Strobilanthes cusia (Nees) Kuntze is the main Indigo source in Landian Yao that is widely used in the world and can be commercially exploited as an Indigo plant. For commercial and environment benefits, we suggest that producing natural Indigo for the commercial market is a good choice.
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Identity blues: the ethnobotany of the Indigo Dyeing by Landian Yao (Iu Mien) in Yunnan, Southwest China
BMC, 2019Co-Authors: Anthony B Cunningham, Ruyan Fan, Yuhua WangAbstract:Abstract Background Indigo-Dyed textiles have been central to the cultural identity of Landian Yao (literally “blue clothes Yao”) people in Southwest China for centuries, driving a significant local market for naturally Dyed Indigo cloth. In the past two decades, local Indigo production for traditional textiles has declined for several reasons: Firstly, the younger generation of Landian Yao has shifted to using western style jeans and T-shirts. Secondly, due to its labor-intensive nature. In contrast, at a global scale, including in China, there has been a revival of interest in natural Indigo use. This is due to a growing awareness in the fashion industry about human and environmental health issues related to synthetic Dye production. Ironically, this new awareness comes at a time when traditional knowledge of Indigo Dyeing is being lost in many places in China, with weaving and use of natural Dyes now limited to some remote areas. In this study, we recorded Indigo Dyeing processes used by Landian Yao people and documented the plant species used for Indigo Dyeing. Methods Field surveys were conducted to the study area from September 2015 to November 2016, supplemented by follow-up visits in July 2018 and November 2018. We interviewed 46 key informants between 36 and 82 years old who still continued traditional Indigo Dyeing practices. Most were elderly people. Semi-structured interviews were used. During the field study, we kept a detailed account of the methods used by Landian Yao Dyers. The data were then analyzed by using utilization frequency to determine the best traditional recipe of Indigo Dye extraction. All the specimens of documented species were collected and deposited at the herbarium of Kunming Institute of Botany. Results Our results showed that Indigo Dyeing was divided into two main steps: (1) Indigo pigment extraction and (2) Dyeing cloth. The general procedures of Indigo Dye extraction included building or buying a Dye vat, fermentation, removal of the leaves of Indigo producing plant species, addition of lime, oxygenation, followed by collection, and the storage of the Indigo paste. The procedures of Dyeing cloth included preparing the Dye solutions, Dyeing cloth, washing, and air drying. It is notable that Landian Yao Dyers formerly only performed the Dyeing process on the goat days in the lunar calendar from June to October. After comparing the range of local Indigo extraction methods, our results showed that the following was best of these traditional recipes: a Indigo-yielding plant material to tap water ratio of 30 kg: 200 l, lime 3 kg, a fermentation time of 2–3 d, aeration by agitation for up to 60 min, and a precipitation time of 2–3 h. Our results show that 17 plant species in 11 families were recorded in the Indigo Dyeing process. With the exception of the Indigo sources, only Dioscorea cirrhosa Lour. and Artemisia argyi H.Lév. & Vaniot were previously recorded in Dyeing processes. Other species given in this paper are recorded for the first time in terms of their use in the Indigo Dyeing process. In the study area, Landian Yao men were in charge of Indigo Dye extraction, and the women were responsible for Dyeing cloth. Conclusions The Landian Yao has completely mastered the traditional Indigo Dyeing craft and are one of the well-deserved identity blues. Indigo production from plants using traditional methods is a slow process compared to synthetic Dyes and is not suitable for modern and rapid industrial production. Therefore, our study records the detailed information of traditional Indigo Dyeing to protect and inherit it. Strobilanthes cusia (Nees) Kuntze is the main Indigo source in Landian Yao that is widely used in the world and can be commercially exploited as an Indigo plant. For commercial and environment benefits, we suggest that producing natural Indigo for the commercial market is a good choice
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Functional Organic Semiconductors Based on Bay-Annulated Indigo (BAI).
eScholarship University of California, 2019Co-Authors: Kolaczkowski, Matthew A, Yi LiuAbstract:The advancement of organic electronics has been continually pushed by the need for stable and high performance acceptor materials. By utilizing inexpensive and stable Indigo Dye as a starting material, Bay-Annulated Indigo (BAI) provides a new motif for the development of semiconducting materials. Modular and straightforward synthesis makes BAI an outstanding platform for molecular design, while excellent stability, strong absorption, and high ambipolar mobility render BAI-based materials excellent candidates for organic electronics. BAI-based polymers and small molecules have taken advantage of these properties to show promising results in a variety of applications
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new form of an old natural Dye bay annulated Indigo bai as an excellent electron accepting unit for high performance organic semiconductors
Journal of the American Chemical Society, 2014Co-Authors: Andrew B Pun, Danylo Zherebetskyy, Yao Liu, Feng Liu, Liana M Klivansky, Alexandra M Mcgough, Benjamin A Zhang, Thomas P Russell, Linwang Wang, Yi LiuAbstract:A novel electron acceptor was synthesized from one-step functionalization of the readily available Indigo Dye. The resulting bay-annulated Indigo (BAI) was utilized for the preparation of a series of novel donor–acceptor small molecules and polymers. As revealed experimentally and by theoretical calculations, substituted BAIs have stronger electron accepting characteristics when compared to several premier electron deficient building blocks. As a result, the donor–acceptor materials incorporating BAI acceptor possess low-lying LUMO energy levels and small HOMO–LUMO gaps. In situ grazing incidence wide-angle X-ray scattering studies of the thin films of BAI donor–acceptor polymers indicated improved crystallinity upon thermal treatment. Field effect transistors based on these polymers show excellent ambipolar transporting behavior, with the hole and electron mobilities reaching 1.5 and 0.41 cm2 V–1 s–1, respectively, affirming BAI as a potent electron accepting unit for high performance organic electronic ...