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  • GalanthIndole: a new Indole Alkaloid from Galanthus plicatus ssp. byzantinus.
    Planta Medica, 2003
    Co-Authors: Nehir Unver, G. Irem Kaya, Christa Werner, Robert Verpoorte, Robert Verpoorte, Belkis Gözler
    Abstract:

    A new Indole Alkaloid, galanthIndole, was isolated from Galanthus plicatus ssp. byzantinus (Amaryllidaceae), a plant native to northwestern Turkey. Incorporating a non-fused Indole ring, galanthIndole may represent the prototype of a new subgroup of the Amaryllidaceae Alkaloids. Two other bases, (+)-11-hydroxyvittatine and hordenine, are also reported from the same plant.

  • Geraniol 10-hydroxylase1, a cytochrome P450 enzyme involved in terpenoid Indole Alkaloid biosynthesis
    FEBS letters, 2001
    Co-Authors: Graziella Collu, Nehir Unver, Robert Van Der Heijden, Robert Verpoorte, Anja M.g. Peltenburg-looman, Robert Verpoorte, Johan Memelink
    Abstract:

    Geraniol 10-hydroxylase (G10H) is a cytochrome P450 monooxygenase involved in the biosynthesis of iridoid monoterpenoids and several classes of monoterpenoid Alkaloids found in a diverse range of plant species. Catharanthus roseus (Madagascar periwinkle) contains monoterpenoid Indole Alkaloids, several of which are pharmaceutically important. Vinblastine and vincristine, for example, find widespread use as anti-cancer drugs. G10H is thought to play a key regulatory role in terpenoid Indole Alkaloid biosynthesis. We purified G10H from C. roseus cells. Using degenerate PCR primers based on amino acid sequence information we cloned the corresponding cDNA. The encoded CYP76B6 protein has G10H activity when expressed in C. roseus and yeast cells. The stress hormone methyljasmonate strongly induced G10h gene expression coordinately with other terpenoid Indole Alkaloid biosynthesis genes in a C. roseus cell culture.