The Experts below are selected from a list of 1620 Experts worldwide ranked by ideXlab platform
Zhen Yang - One of the best experts on this subject based on the ideXlab platform.
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regiospecific carbonylative annulation of iodophenol acetates and acetylenes to construct the flavones by a new catalyst of palladium thiourea dppp complex
Organic Letters, 2000Co-Authors: Hua Miao, Zhen YangAbstract:Regiospecific carbonylative annulation of o-iodophenol acetates and acetylenes mediated by palladium−thiourea−dppp complex in the presence of base at 40 °C under a balloon pressure of CO generates diversified flavones in high yields. This newly developed synthetic technology provides a highly efficient method for potential application to the combinatorial synthesis of those heterocycles on the solid support.
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regiospecific carbonylative annulation of iodophenol acetates and acetylenes to construct the flavones by a new catalyst of palladium thiourea dppp complex
Organic Letters, 2000Co-Authors: Hua Miao, Zhen YangAbstract:[reaction: see text] Regiospecific carbonylative annulation of o-iodophenol acetates and acetylenes mediated by palladium-thiourea-dppp complex in the presence of base at 40 degrees C under a balloon pressure of CO generates diversified flavones in high yields. This newly developed synthetic technology provides a highly efficient method for potential application to the combinatorial synthesis of those heterocycles on the solid support.
Hua Miao - One of the best experts on this subject based on the ideXlab platform.
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regiospecific carbonylative annulation of iodophenol acetates and acetylenes to construct the flavones by a new catalyst of palladium thiourea dppp complex
Organic Letters, 2000Co-Authors: Hua Miao, Zhen YangAbstract:Regiospecific carbonylative annulation of o-iodophenol acetates and acetylenes mediated by palladium−thiourea−dppp complex in the presence of base at 40 °C under a balloon pressure of CO generates diversified flavones in high yields. This newly developed synthetic technology provides a highly efficient method for potential application to the combinatorial synthesis of those heterocycles on the solid support.
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regiospecific carbonylative annulation of iodophenol acetates and acetylenes to construct the flavones by a new catalyst of palladium thiourea dppp complex
Organic Letters, 2000Co-Authors: Hua Miao, Zhen YangAbstract:[reaction: see text] Regiospecific carbonylative annulation of o-iodophenol acetates and acetylenes mediated by palladium-thiourea-dppp complex in the presence of base at 40 degrees C under a balloon pressure of CO generates diversified flavones in high yields. This newly developed synthetic technology provides a highly efficient method for potential application to the combinatorial synthesis of those heterocycles on the solid support.
Dawei Zhang - One of the best experts on this subject based on the ideXlab platform.
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RESEARCH ARTICLE An Enzymatic Assay for High-Throughput Screening of Cytidine-Producing Microbial Strains
2016Co-Authors: Huina Dong, Yongfei Liu, Dawei ZhangAbstract:Cytidine is an industrially useful precursor for the production of antiviral compounds and a variety of industrial compounds. Interest in the microbial production of cytidine has grown recently and high-throughput screening of cytidine over-producers is an important approach in large-scale industrial production using microorganisms. An enzymatic assay for cytidine was developed combining cytidine deaminase (CDA) and Indophenol method. CDA cata-lyzes the cleavage of cytidine to uridine and NH3, the latter of which can be accurately deter-mined using the Indophenol method. The assay was performed in 96-well plates and had a linear detection range of cytidine of 0.058- 10 mM. This assay was used to determine the amount of cytidine in fermentation flasks and the results were compared with that of High Perfomance Liquid Chromatography (HPLC) method. The detection range of the CDA method is not as wide as that of the HPLC, furthermore the correlation factor of CDAmethod is not as high as that of HPLC. However, it was suitable for the detection of large numbers of crude samples and was applied to high-throughput screening for high cytidine-producing strains using 96-well deep-hole culture plates. This assay was proved to be simple, accu-rate, specific and suitable for cytidine detection and high-throughput screening of cytidine-producing strains in large numbers of samples (96 well or more)
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An enzymatic assay for high-throughput screening of cytidine-producing microbial strains.
PloS one, 2015Co-Authors: Huina Dong, Yongfei Liu, Dawei ZhangAbstract:Cytidine is an industrially useful precursor for the production of antiviral compounds and a variety of industrial compounds. Interest in the microbial production of cytidine has grown recently and high-throughput screening of cytidine over-producers is an important approach in large-scale industrial production using microorganisms. An enzymatic assay for cytidine was developed combining cytidine deaminase (CDA) and Indophenol method. CDA catalyzes the cleavage of cytidine to uridine and NH3, the latter of which can be accurately determined using the Indophenol method. The assay was performed in 96-well plates and had a linear detection range of cytidine of 0.058 - 10 mM. This assay was used to determine the amount of cytidine in fermentation flasks and the results were compared with that of High Perfomance Liquid Chromatography (HPLC) method. The detection range of the CDA method is not as wide as that of the HPLC, furthermore the correlation factor of CDA method is not as high as that of HPLC. However, it was suitable for the detection of large numbers of crude samples and was applied to high-throughput screening for high cytidine-producing strains using 96-well deep-hole culture plates. This assay was proved to be simple, accurate, specific and suitable for cytidine detection and high-throughput screening of cytidine-producing strains in large numbers of samples (96 well or more).
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a rapid enzymatic assay for high throughput screening of adenosine producing strains
Microbial Biotechnology, 2015Co-Authors: Huina Dong, Ping Zheng, Dawei ZhangAbstract:Adenosine is a major local regulator of tissue function and industrially useful as precursor for the production of medicinal nucleoside substances. High-throughput screening of adenosine overproducers is important for industrial microorganism breeding. An enzymatic assay of adenosine was developed by combined adenosine deaminase (ADA) with Indophenol method. The ADA catalyzes the cleavage of adenosine to inosine and NH3, the latter can be accurately determined by Indophenol method. The assay system was optimized to deliver a good performance and could tolerate the addition of inorganic salts and many nutrition components to the assay mixtures. Adenosine could be accurately determined by this assay using 96-well microplates. Spike and recovery tests showed that this assay can accurately and reproducibly determine increases in adenosine in fermentation broth without any pretreatment to remove proteins and potentially interfering low-molecular-weight molecules. This assay was also applied to high-throughput screening for high adenosine-producing strains. The high selectivity and accuracy of the ADA assay provides rapid and high-throughput analysis of adenosine in large numbers of samples.
Venkatesh Devaru - One of the best experts on this subject based on the ideXlab platform.
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synthesis structure activity relationship of iodinated 4 aryloxymethyl coumarins as potential anti cancer and anti mycobacterial agents
European Journal of Medicinal Chemistry, 2014Co-Authors: Mahantesha Basanagouda, Vishwanath B Jambagi, Nivedita N Barigidad, Sandeep S Laxmeshwar, Venkatesh DevaruAbstract:Abstract A series of new iodinated-4-aryloxymethylcoumarins 6, 8 and 10 have been obtained from the reaction of various 4-bromomethylcoumarins 4 with 2-iodophenol 5, 3-iodophenol 7 and 4-iodophenol 9 respectively. All the title compounds were screened for anticancer activity against two cancer cell lines (MDA-MB human adenocarcinoma mammary gland and A-549 human lung carcinoma) and two mycobacterial strains (Mycobacterium tuberculosis H37 RV and Mycobacterium phlei). The SAR results indicate that nine compounds are potent, among these 10h and 10i having chlorine are most effective. This is the first report assigning in vitro anti-mycobacterial, anticancer and structure–activity relationship for this new class of iodinated-4-aryloxymethyl-coumarins.
Huina Dong - One of the best experts on this subject based on the ideXlab platform.
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RESEARCH ARTICLE An Enzymatic Assay for High-Throughput Screening of Cytidine-Producing Microbial Strains
2016Co-Authors: Huina Dong, Yongfei Liu, Dawei ZhangAbstract:Cytidine is an industrially useful precursor for the production of antiviral compounds and a variety of industrial compounds. Interest in the microbial production of cytidine has grown recently and high-throughput screening of cytidine over-producers is an important approach in large-scale industrial production using microorganisms. An enzymatic assay for cytidine was developed combining cytidine deaminase (CDA) and Indophenol method. CDA cata-lyzes the cleavage of cytidine to uridine and NH3, the latter of which can be accurately deter-mined using the Indophenol method. The assay was performed in 96-well plates and had a linear detection range of cytidine of 0.058- 10 mM. This assay was used to determine the amount of cytidine in fermentation flasks and the results were compared with that of High Perfomance Liquid Chromatography (HPLC) method. The detection range of the CDA method is not as wide as that of the HPLC, furthermore the correlation factor of CDAmethod is not as high as that of HPLC. However, it was suitable for the detection of large numbers of crude samples and was applied to high-throughput screening for high cytidine-producing strains using 96-well deep-hole culture plates. This assay was proved to be simple, accu-rate, specific and suitable for cytidine detection and high-throughput screening of cytidine-producing strains in large numbers of samples (96 well or more)
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An enzymatic assay for high-throughput screening of cytidine-producing microbial strains.
PloS one, 2015Co-Authors: Huina Dong, Yongfei Liu, Dawei ZhangAbstract:Cytidine is an industrially useful precursor for the production of antiviral compounds and a variety of industrial compounds. Interest in the microbial production of cytidine has grown recently and high-throughput screening of cytidine over-producers is an important approach in large-scale industrial production using microorganisms. An enzymatic assay for cytidine was developed combining cytidine deaminase (CDA) and Indophenol method. CDA catalyzes the cleavage of cytidine to uridine and NH3, the latter of which can be accurately determined using the Indophenol method. The assay was performed in 96-well plates and had a linear detection range of cytidine of 0.058 - 10 mM. This assay was used to determine the amount of cytidine in fermentation flasks and the results were compared with that of High Perfomance Liquid Chromatography (HPLC) method. The detection range of the CDA method is not as wide as that of the HPLC, furthermore the correlation factor of CDA method is not as high as that of HPLC. However, it was suitable for the detection of large numbers of crude samples and was applied to high-throughput screening for high cytidine-producing strains using 96-well deep-hole culture plates. This assay was proved to be simple, accurate, specific and suitable for cytidine detection and high-throughput screening of cytidine-producing strains in large numbers of samples (96 well or more).
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a rapid enzymatic assay for high throughput screening of adenosine producing strains
Microbial Biotechnology, 2015Co-Authors: Huina Dong, Ping Zheng, Dawei ZhangAbstract:Adenosine is a major local regulator of tissue function and industrially useful as precursor for the production of medicinal nucleoside substances. High-throughput screening of adenosine overproducers is important for industrial microorganism breeding. An enzymatic assay of adenosine was developed by combined adenosine deaminase (ADA) with Indophenol method. The ADA catalyzes the cleavage of adenosine to inosine and NH3, the latter can be accurately determined by Indophenol method. The assay system was optimized to deliver a good performance and could tolerate the addition of inorganic salts and many nutrition components to the assay mixtures. Adenosine could be accurately determined by this assay using 96-well microplates. Spike and recovery tests showed that this assay can accurately and reproducibly determine increases in adenosine in fermentation broth without any pretreatment to remove proteins and potentially interfering low-molecular-weight molecules. This assay was also applied to high-throughput screening for high adenosine-producing strains. The high selectivity and accuracy of the ADA assay provides rapid and high-throughput analysis of adenosine in large numbers of samples.