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Mausam P. Verma - One of the best experts on this subject based on the ideXlab platform.
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hydrolytic pre treatment methods for enhanced biobutanol production from agro Industrial Wastes
Bioresource Technology, 2018Co-Authors: Sampa Maiti, Satinder Kaur Brar, Saurabh Jyoti Sarma, Patrick Drogui, Gerardo Buelna, Gorka Gallastegui, Gayatri Suresh, Yann Lebihan, Mausam P. VermaAbstract:Abstract Brewery industry liquid waste (BLW), brewery spent grain (BSG), apple pomace solid Wastes (APS), apple pomace ultrafiltration sludge (APUS) and starch industry wastewater (SIW) have been considered as substrates to produce biobutanol. Efficiency of hydrolysis techniques tested to produce fermentable sugars depended on nature of agro-Industrial Wastes and process conditions. Acid-catalysed hydrolysis of BLW and BSG gave a total reducing sugar yield of 0.433 g/g and 0.468 g/g respectively. Reducing sugar yield from microwave assisted hydrothermal method was 0.404 g/g from APS and 0.631 g/g from APUS, and, 0.359 g/g from microwave assisted acid-catalysed SIW dry mass. Parameter optimization (time, pH and substrate concentration) for acid-catalysed BLW hydrolysate utilization using central composite model technique produced 307.9 g/kg glucose with generation of inhibitors (5-hydroxymethyl furfural (20 g/kg), furfural (1.6 g/kg), levulinic acid (9.3 g/kg) and total phenolic compound (0.567 g/kg)). 10.62 g/L of acetone-butanol-ethanol was produced by subsequent clostridial fermentation of the substrate.
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agro Industrial Wastes as feedstock for sustainable bio production of butanol by clostridium beijerinckii
Food and Bioproducts Processing, 2016Co-Authors: Sampa Maiti, Satinder Kaur Brar, Saurabh Jyoti Sarma, Yann Le Bihan, Patrick Drogui, Gerardo Buelna, Mausam P. VermaAbstract:Abstract Three different inexpensive, abundant agro-Industrial Wastes: suspended brewery liquid waste (BLW), starch industry wastewater (SIW) and apple pomace ultra-filtration sludge (APUS) have been explored for biobutanol production. Physicochemical analysis of biomass for carbohydrate and nutrient pool, acid hydrolysis, fermentation of raw extracts and hydrolysates by Clostridium beijerinckii NRRL B-466 for ABE production and glucose supplementation to increase butanol production have been studied. The efficiency of the microorganism to produce butanol and other metabolites was explored via carbon balance. Inhibitory effects were minimized by dilution and detoxification method. Pretreated diluted extracts having reducing sugar content of 30 g/L gave optimum butanol production of 4.68 g/L, 1.4 g/L and 1.8 g/L from SIWH, APUSH and BLWH, respectively, as compared to 5.1 g/L obtained from control. Reducing sugar concentration in each diluted extract was increased to 60 g/L by 3% (w/v) glucose supplement and the butanol production was increased to 11.04 g/L (0.27 g/g), 9.3 g/L (0.24 g/g) and 8.06 g/L (0.25), respectively. For SIWH, the maximum yield of butanol (0.27 g/g) and acetone–butanol–ethanol (ABE) (0.46 g/g) was obtained. Thus, production of butanol, with 3% glucose supplement demonstrated that these inexpensive agro-Industrial Wastes could have important implications in stimulating energy-economics.
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screening of agro Industrial Wastes for citric acid bioproduction by aspergillus niger nrrl 2001 through solid state fermentation
Journal of the Science of Food and Agriculture, 2013Co-Authors: Gurpreet Singh Dhillon, Surinder Kaur, Satinder Kaur Brar, Mausam P. VermaAbstract:Background: The citric acid (CA) industry is currently struggling to develop a sustainable and economical process owing to high substrate and energy costs. Increasing interest in the replacement of costly synthetic substrates by renewable waste biomass has fostered research on agro-Industrial Wastes and screening of raw materials for economical CA production. The food-processing industry generates substantial quantities of waste biomass that could be used as a valuable low-cost fermentation substrate. The present study evaluated the potential of different agro-Industrial Wastes, namely apple pomace (AP), brewer's spent grain, citrus waste and sphagnum peat moss, as substrates for solid state CA production using Aspergillus niger NRRL 2001. Results: Among the four substrates, AP resulted in highest CA production of 61.06 ± 1.9 g kg-1 dry substrate (DS) after a 72 h incubation period. Based on the screening studies, AP was selected for optimisation studies through response surface methodology (RSM). Maximum CA production of 312.32 g kg-1 DS was achieved at 75% (v/w) moisture and 3% (v/w) methanol after a 144 h incubation period. The validation of RSM-optimised parameters in plastic trays resulted in maximum CA production of 364.4 ± 4.50 g kg-1 DS after a 120 h incubation period. Conclusion: The study demonstrated the potential of AP as a cheap substrate for higher CA production. This study contributes to knowledge about the future application of carbon rich agro-Industrial Wastes for their value addition to CA. It also offers economic and environmental benefits over traditional ways used to dispose off agro-Industrial Wastes.
Bernard Scanlon - One of the best experts on this subject based on the ideXlab platform.
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pullulan content of the ethanol precipitate from fermented agro Industrial Wastes
Applied Microbiology and Biotechnology, 1998Co-Authors: C Israilides, Alan Smith, J E Harthill, Christian Barnett, G Bambalov, Bernard ScanlonAbstract:Ethanol-precipitated substances after fermentation of various agro-Industrial Wastes by Aureobasidium pullulans were examined for their pullulan content. Grape skin pulp extract, starch waste, olive oil waste effluents and molasses served as substrates for the fermentation. A glucose-based defined medium was used for comparison purposes. Samples were analysed by an enzyme-coupled assay method and by high-performance anion-exchange chromatography with pulsed amperometric detection after enzymic hydrolysis with pullulanase. Fermentation of grape skin pulp extract gave 22.3 g l−1 ethanol precipitate, which was relatively pure pullulan (97.4% w/w) as assessed by the coupled-enzyme assay. Hydrolysed starch gave only 12.9 g l−1 ethanol precipitate, which increased to 30.8 g l−1 when the medium was supplemented with NH4NO3 and K2HPO4; this again was relatively pure pullulan (88.6% w/w). Molasses and olive oil Wastes produced heterogeneous ethanol-precipitated substances containing small amounts of pullulan, even when supplemented with nitrogen and phosphate. Overall, grape skin pulp should be considered as the best substrate for pullulan production. Starch waste requires several hydrolyis steps to provide a usable carbon source, which reduces its economic attraction as an Industrial process.
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characterization of pullulans produced from agro Industrial Wastes
Carbohydrate Polymers, 1994Co-Authors: C Israilides, Bernard Scanlon, Alan Smith, Stephen E Harding, Kornelia JumelAbstract:Abstract Pullulans produced from the Aureobasidium pullulans fermentation of various agro-Industrial Wastes were characterised in terms of carbohydrate analysis. heterogeneity and molecular weights. Grape skin pulp extract (GSPE), starch waste (SW), olive oil waste effluents (OW) and molasses (M) were used as substrates for the production of pullulan. A glucose based defined medium (D) was used for comparison purposes. Weight average molecular weights were determined using size exclusion chromatography/multiangle laser light scattering. In each of the ethanol precipitated substances the molecular weights were higher than that of the reference pullulan (Sigma), and the elution profiles indicated the polydisperse nature of the pullulans. When using D, the molecular weight of pullulan produced decreased with increasing fermentation time. Different substrates produced pullulans with different molecular weights. Of the substrates studied, D, GSPE and SW produced pullulans in high yields, which were relatively homogeneous, while OW alone or with M produced greatly heterogeneous ethanol agglutinating substances.
J A Teixeira - One of the best experts on this subject based on the ideXlab platform.
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use of agro Industrial Wastes in solid state fermentation processes
2012Co-Authors: Solange Inês Mussatto, Lina F Ballesteros, Silvia Martins, J A TeixeiraAbstract:Large amount of Wastes is generated every year from the Industrial processing of agricultural raw materials. Most of these Wastes are used as animal feed or burned as alternative for elimination. However, such Wastes usually have a composition rich in sugars, minerals and proteins, and therefore, they should not be considered “Wastes” but raw materials for other Industrial processes. The presence of carbon sources, nutrients and moisture in these Wastes provides conditions suitable for the development of microorganisms, and this open up great possibilities for their reuse in solid-state fermentation (SSF) processes, for example. Agro-Industrial Wastes can be used as solid support, carbon and/or nutrient source in SSF processes for the production of a variety of value-added compounds.
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exploitation of agro Industrial Wastes as immobilization carrier for solid state fermentation
Industrial Crops and Products, 2009Co-Authors: Maria C Orzua, Solange Inês Mussatto, Juan C Contrerasesquivel, Raul Rodriguez, Heliodoro De La Garza, J A Teixeira, Cristobal N AguilarAbstract:Ten agro Industrial Wastes were assessed for their suitability as fungus immobilization carrier for solid-state fermentation (SSF). The Wastes included creosote bush leaves (Larrea tridentata), variegated Caribbean agave (Agave lechuguilla), lemon peel (Citrus aurantifolia), orange peel (Citrus sinensis), apple pomace (Malus domestica), pistachio shell (Pistacia vera), wheat bran (Triticum spp.), coconut husk (Cocos nucifera), pecan nutshell (Carya illinoinensis), and bean residues (Phaseolus vulgaris). All of them were physical–chemically and microbiologically characterized. Physical–chemical tests consisted in the determination of the critical humidity point and the water absorption index; while the microbiological tests were based on the evaluation of Aspergillus niger Aa-20 growth rate in such materials. The study pointed out that coconut husk, apple pomace, lemon and orange peels were the materials of greater potential for use as immobilization carrier in SSF, since they have high water absorption capacity, and allowed good microorganism growth rate. This result is of significant interest due to the low cost and abundant availability of such Wastes.
C Israilides - One of the best experts on this subject based on the ideXlab platform.
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pullulan content of the ethanol precipitate from fermented agro Industrial Wastes
Applied Microbiology and Biotechnology, 1998Co-Authors: C Israilides, Alan Smith, J E Harthill, Christian Barnett, G Bambalov, Bernard ScanlonAbstract:Ethanol-precipitated substances after fermentation of various agro-Industrial Wastes by Aureobasidium pullulans were examined for their pullulan content. Grape skin pulp extract, starch waste, olive oil waste effluents and molasses served as substrates for the fermentation. A glucose-based defined medium was used for comparison purposes. Samples were analysed by an enzyme-coupled assay method and by high-performance anion-exchange chromatography with pulsed amperometric detection after enzymic hydrolysis with pullulanase. Fermentation of grape skin pulp extract gave 22.3 g l−1 ethanol precipitate, which was relatively pure pullulan (97.4% w/w) as assessed by the coupled-enzyme assay. Hydrolysed starch gave only 12.9 g l−1 ethanol precipitate, which increased to 30.8 g l−1 when the medium was supplemented with NH4NO3 and K2HPO4; this again was relatively pure pullulan (88.6% w/w). Molasses and olive oil Wastes produced heterogeneous ethanol-precipitated substances containing small amounts of pullulan, even when supplemented with nitrogen and phosphate. Overall, grape skin pulp should be considered as the best substrate for pullulan production. Starch waste requires several hydrolyis steps to provide a usable carbon source, which reduces its economic attraction as an Industrial process.
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characterization of pullulans produced from agro Industrial Wastes
Carbohydrate Polymers, 1994Co-Authors: C Israilides, Bernard Scanlon, Alan Smith, Stephen E Harding, Kornelia JumelAbstract:Abstract Pullulans produced from the Aureobasidium pullulans fermentation of various agro-Industrial Wastes were characterised in terms of carbohydrate analysis. heterogeneity and molecular weights. Grape skin pulp extract (GSPE), starch waste (SW), olive oil waste effluents (OW) and molasses (M) were used as substrates for the production of pullulan. A glucose based defined medium (D) was used for comparison purposes. Weight average molecular weights were determined using size exclusion chromatography/multiangle laser light scattering. In each of the ethanol precipitated substances the molecular weights were higher than that of the reference pullulan (Sigma), and the elution profiles indicated the polydisperse nature of the pullulans. When using D, the molecular weight of pullulan produced decreased with increasing fermentation time. Different substrates produced pullulans with different molecular weights. Of the substrates studied, D, GSPE and SW produced pullulans in high yields, which were relatively homogeneous, while OW alone or with M produced greatly heterogeneous ethanol agglutinating substances.
Solange Inês Mussatto - One of the best experts on this subject based on the ideXlab platform.
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use of agro Industrial Wastes in solid state fermentation processes
2012Co-Authors: Solange Inês Mussatto, Lina F Ballesteros, Silvia Martins, J A TeixeiraAbstract:Large amount of Wastes is generated every year from the Industrial processing of agricultural raw materials. Most of these Wastes are used as animal feed or burned as alternative for elimination. However, such Wastes usually have a composition rich in sugars, minerals and proteins, and therefore, they should not be considered “Wastes” but raw materials for other Industrial processes. The presence of carbon sources, nutrients and moisture in these Wastes provides conditions suitable for the development of microorganisms, and this open up great possibilities for their reuse in solid-state fermentation (SSF) processes, for example. Agro-Industrial Wastes can be used as solid support, carbon and/or nutrient source in SSF processes for the production of a variety of value-added compounds.
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exploitation of agro Industrial Wastes as immobilization carrier for solid state fermentation
Industrial Crops and Products, 2009Co-Authors: Maria C Orzua, Solange Inês Mussatto, Juan C Contrerasesquivel, Raul Rodriguez, Heliodoro De La Garza, J A Teixeira, Cristobal N AguilarAbstract:Ten agro Industrial Wastes were assessed for their suitability as fungus immobilization carrier for solid-state fermentation (SSF). The Wastes included creosote bush leaves (Larrea tridentata), variegated Caribbean agave (Agave lechuguilla), lemon peel (Citrus aurantifolia), orange peel (Citrus sinensis), apple pomace (Malus domestica), pistachio shell (Pistacia vera), wheat bran (Triticum spp.), coconut husk (Cocos nucifera), pecan nutshell (Carya illinoinensis), and bean residues (Phaseolus vulgaris). All of them were physical–chemically and microbiologically characterized. Physical–chemical tests consisted in the determination of the critical humidity point and the water absorption index; while the microbiological tests were based on the evaluation of Aspergillus niger Aa-20 growth rate in such materials. The study pointed out that coconut husk, apple pomace, lemon and orange peels were the materials of greater potential for use as immobilization carrier in SSF, since they have high water absorption capacity, and allowed good microorganism growth rate. This result is of significant interest due to the low cost and abundant availability of such Wastes.