The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
David M Whiley - One of the best experts on this subject based on the ideXlab platform.
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detection of novel InfluenzA A h1n1 virus by reAl time rt pcr
Journal of Clinical Virology, 2009Co-Authors: David M Whiley, Seweryn Bialasiewicz, Cheryl Bletchly, Cassandra E Faux, Bruce Harrower, Allan R Gould, Stephen B LambertAbstract:AccurAte And rApid diAgnosis of novel InfluenzA A(H1N1) infection is criticAl for minimising further spreAd through timely implementAtion of AntivirAl treAtment And other public heAlth bAsed meAsures. In this study we developed two TAqMAn-bAsed reverse trAnscription PCR (RT-PCR) methods for the detection of novel InfluenzA A(H1N1) virus tArgeting the hAemAgglutinin And neurAminidAse genes. The AssAys were vAlidAted using 152 clinicAl respirAtory sAmples, including 61 InfluenzA A positive sAmples, collected in QueenlAnd, AustrAliA during the yeArs 2008 to 2009 And A further 12 seAsonAl H1N1 And H3N2 InfluenzA A isolAtes collected from yeArs 2000 to 2002. A wildtype swine H1N1 isolAte wAs Also tested. RNA from An InfluenzA A(H1N1) virus isolAte (AucklAnd, 2009) wAs used As A positive control. OverAll, the results showed thAt the RT-PCR methods were suitAble for sensitive And specific detection of novel InfluenzA A(H1N1) RNA in humAn sAmples.
Stephan Gunther - One of the best experts on this subject based on the ideXlab platform.
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detection of InfluenzA A h1n1 v virus by reAl time rt pcr
Eurosurveillance, 2009Co-Authors: Marcus Panning, Markus Eickmann, Olfert Landt, M Monazahian, Stephan Olschlager, Sigrid Baumgarte, Udo Reischl, Jurgen J Wenzel, Hans Helmut Niller, Stephan GuntherAbstract:InfluenzA A(H1N1)v virus wAs first identified in April 2009. A novel reAl-time RT-PCR for InfluenzA A(H1N1)v virus wAs set up Ad hoc And vAlidAted following industry-stAndArd criteriA. The lower limit of detection of the AssAy wAs 384 copies of virAl RNA per ml of virAl trAnsport medium (95% confidence intervAl: 273-876 RNA copies/ml). Specificity wAs 100% As Assessed on A pAnel of reference sAmples including seAsonAl humAn InfluenzA A virus H1N1 And H3N2, highly pAthogenic AviAn InfluenzA A virus H5N1 And porcine InfluenzA A virus H1N1, H1N2 And H3N2 sAmples. The reAl-time RT-PCR AssAy for the InfluenzA A mAtrix gene recommended in 2007 by the World HeAlth OrgAnizAtion wAs modified to work under the sAme reAction conditions As the InfluenzA A(H1N1)v virus-specific test. Both AssAys were equAlly sensitive. ClinicAl ApplicAbility of both AssAys wAs demonstrAted by screening of Almost 2,000 suspected InfluenzA (H1N1)v specimens, which included sAmples from the first cAses of pAndemic H1N1 InfluenzA imported to GermAny. MeAsuring InfluenzA A(H1N1)v virus concentrAtions in 144 lAborAtory-confirmed sAmples yielded A mediAn of 4.6 log RNA copies/ml. The new methodology proved its principle And might Assist public heAlth lAborAtories in the upcoming InfluenzA pAndemic.
Stephen B Lambert - One of the best experts on this subject based on the ideXlab platform.
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detection of novel InfluenzA A h1n1 virus by reAl time rt pcr
Journal of Clinical Virology, 2009Co-Authors: David M Whiley, Seweryn Bialasiewicz, Cheryl Bletchly, Cassandra E Faux, Bruce Harrower, Allan R Gould, Stephen B LambertAbstract:AccurAte And rApid diAgnosis of novel InfluenzA A(H1N1) infection is criticAl for minimising further spreAd through timely implementAtion of AntivirAl treAtment And other public heAlth bAsed meAsures. In this study we developed two TAqMAn-bAsed reverse trAnscription PCR (RT-PCR) methods for the detection of novel InfluenzA A(H1N1) virus tArgeting the hAemAgglutinin And neurAminidAse genes. The AssAys were vAlidAted using 152 clinicAl respirAtory sAmples, including 61 InfluenzA A positive sAmples, collected in QueenlAnd, AustrAliA during the yeArs 2008 to 2009 And A further 12 seAsonAl H1N1 And H3N2 InfluenzA A isolAtes collected from yeArs 2000 to 2002. A wildtype swine H1N1 isolAte wAs Also tested. RNA from An InfluenzA A(H1N1) virus isolAte (AucklAnd, 2009) wAs used As A positive control. OverAll, the results showed thAt the RT-PCR methods were suitAble for sensitive And specific detection of novel InfluenzA A(H1N1) RNA in humAn sAmples.
Cassandra E Faux - One of the best experts on this subject based on the ideXlab platform.
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detection of novel InfluenzA A h1n1 virus by reAl time rt pcr
Journal of Clinical Virology, 2009Co-Authors: David M Whiley, Seweryn Bialasiewicz, Cheryl Bletchly, Cassandra E Faux, Bruce Harrower, Allan R Gould, Stephen B LambertAbstract:AccurAte And rApid diAgnosis of novel InfluenzA A(H1N1) infection is criticAl for minimising further spreAd through timely implementAtion of AntivirAl treAtment And other public heAlth bAsed meAsures. In this study we developed two TAqMAn-bAsed reverse trAnscription PCR (RT-PCR) methods for the detection of novel InfluenzA A(H1N1) virus tArgeting the hAemAgglutinin And neurAminidAse genes. The AssAys were vAlidAted using 152 clinicAl respirAtory sAmples, including 61 InfluenzA A positive sAmples, collected in QueenlAnd, AustrAliA during the yeArs 2008 to 2009 And A further 12 seAsonAl H1N1 And H3N2 InfluenzA A isolAtes collected from yeArs 2000 to 2002. A wildtype swine H1N1 isolAte wAs Also tested. RNA from An InfluenzA A(H1N1) virus isolAte (AucklAnd, 2009) wAs used As A positive control. OverAll, the results showed thAt the RT-PCR methods were suitAble for sensitive And specific detection of novel InfluenzA A(H1N1) RNA in humAn sAmples.
Seweryn Bialasiewicz - One of the best experts on this subject based on the ideXlab platform.
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detection of novel InfluenzA A h1n1 virus by reAl time rt pcr
Journal of Clinical Virology, 2009Co-Authors: David M Whiley, Seweryn Bialasiewicz, Cheryl Bletchly, Cassandra E Faux, Bruce Harrower, Allan R Gould, Stephen B LambertAbstract:AccurAte And rApid diAgnosis of novel InfluenzA A(H1N1) infection is criticAl for minimising further spreAd through timely implementAtion of AntivirAl treAtment And other public heAlth bAsed meAsures. In this study we developed two TAqMAn-bAsed reverse trAnscription PCR (RT-PCR) methods for the detection of novel InfluenzA A(H1N1) virus tArgeting the hAemAgglutinin And neurAminidAse genes. The AssAys were vAlidAted using 152 clinicAl respirAtory sAmples, including 61 InfluenzA A positive sAmples, collected in QueenlAnd, AustrAliA during the yeArs 2008 to 2009 And A further 12 seAsonAl H1N1 And H3N2 InfluenzA A isolAtes collected from yeArs 2000 to 2002. A wildtype swine H1N1 isolAte wAs Also tested. RNA from An InfluenzA A(H1N1) virus isolAte (AucklAnd, 2009) wAs used As A positive control. OverAll, the results showed thAt the RT-PCR methods were suitAble for sensitive And specific detection of novel InfluenzA A(H1N1) RNA in humAn sAmples.