The Experts below are selected from a list of 195 Experts worldwide ranked by ideXlab platform
Michael D. Sitrin - One of the best experts on this subject based on the ideXlab platform.
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Inorganic Phosphorus Reduces Hypercalciuria During Total Parenteral Nutrition By Enhancing Renal Tubular Calcium Absorption
JPEN. Journal of parenteral and enteral nutrition, 1998Co-Authors: Charles H. Berkelhammer, Richard J. Wood, Michael D. SitrinAbstract:Background: Increasing the Inorganic Phosphorus content of total parenteral nutrition (TPN) formulas has been shown to decrease TPN-induced hypercalciuria in experimental animals and humans. The mechanism of this effect, however, has been uncertain. Methods: By using a randomized cross-over design, seven patients on cyclic TPN were given otherwise identical formulas providing either 15 or 45 mmol/d of Inorganic Phosphorus. Urinary calcium excretion, serum ultrafilterable calcium, filtered calcium load, fractional calcium excretion, urinary cyclic adenosine 5'-monophosphate (cAMP), and serum levels of ionized calcium, parathyroid hormone (PTH), and vitamin D metabolites were determined at the end of each study period. Results: Urinary calcium excretion was significantly lower when the patients received the higher Inorganic Phosphorus formula. Increasing the Inorganic Phosphorus in the TPN formula did not change ultrafilterable calcium or filtered calcium load, but significantly reduced fractional calcium excretion. No differences in serum levels of ionized calcium, PTH, 25-hydroxyvitamin D, 1,25-dihydroxyvitamin D, or urinary cAMP were observed between treatments. Conclusions: These results demonstrate that increasing the Inorganic Phosphorus content of the TPN formula decreases urinary calcium excretion by increasing renal tubular calcium resorption. This effect is not due to alterations in the PTH-1,25-dihydroxyitamin D axis, but likely reflects a direct action of Inorganic Phosphorus on the renal tubules.
Pin Yang - One of the best experts on this subject based on the ideXlab platform.
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UV-vis spectroscopic study directly detecting Inorganic Phosphorus in urine and our reagent kit.
Analytical and bioanalytical chemistry, 2005Co-Authors: Caixia Yin, Fangjun Huo, Pin YangAbstract:The determination of Inorganic Phosphorus in human urine is very important, since it has diagnostic value in some clinical cases. Here we apply a simple, sensitive and direct method to determine Inorganic Phosphorus in urine. This new ensemble is prepared by adding ytterbium chloride and pyrocatechol violet in a 2:1 molar ratio in an aqueous solution of 10 mM 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid buffer at pH 7.0. The addition of the urine sample turned the blue ensemble yellow and altered the UV-vis absorption spectra. The ensemble exhibits excellent selectivity for Inorganic Phosphorus over other constituents of urine. We validate the accuracy of our method by the standard procedure (molybdenum blue assay for phosphate). The detection results are basically consistent with normal excretion of phosphate. Furthermore, we fabricated a new kind of Inorganic Phosphorus reagent kit, which enables us to inspect phosphate concentrations of urine with the naked eye. Fit for all kinds of various clinic uses, our reagent kit is a hopeful substitute for the molybdenum reagent kit.
Charles H. Berkelhammer - One of the best experts on this subject based on the ideXlab platform.
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Inorganic Phosphorus Reduces Hypercalciuria During Total Parenteral Nutrition By Enhancing Renal Tubular Calcium Absorption
JPEN. Journal of parenteral and enteral nutrition, 1998Co-Authors: Charles H. Berkelhammer, Richard J. Wood, Michael D. SitrinAbstract:Background: Increasing the Inorganic Phosphorus content of total parenteral nutrition (TPN) formulas has been shown to decrease TPN-induced hypercalciuria in experimental animals and humans. The mechanism of this effect, however, has been uncertain. Methods: By using a randomized cross-over design, seven patients on cyclic TPN were given otherwise identical formulas providing either 15 or 45 mmol/d of Inorganic Phosphorus. Urinary calcium excretion, serum ultrafilterable calcium, filtered calcium load, fractional calcium excretion, urinary cyclic adenosine 5'-monophosphate (cAMP), and serum levels of ionized calcium, parathyroid hormone (PTH), and vitamin D metabolites were determined at the end of each study period. Results: Urinary calcium excretion was significantly lower when the patients received the higher Inorganic Phosphorus formula. Increasing the Inorganic Phosphorus in the TPN formula did not change ultrafilterable calcium or filtered calcium load, but significantly reduced fractional calcium excretion. No differences in serum levels of ionized calcium, PTH, 25-hydroxyvitamin D, 1,25-dihydroxyvitamin D, or urinary cAMP were observed between treatments. Conclusions: These results demonstrate that increasing the Inorganic Phosphorus content of the TPN formula decreases urinary calcium excretion by increasing renal tubular calcium resorption. This effect is not due to alterations in the PTH-1,25-dihydroxyitamin D axis, but likely reflects a direct action of Inorganic Phosphorus on the renal tubules.
Caixia Yin - One of the best experts on this subject based on the ideXlab platform.
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UV-vis spectroscopic study directly detecting Inorganic Phosphorus in urine and our reagent kit.
Analytical and bioanalytical chemistry, 2005Co-Authors: Caixia Yin, Fangjun Huo, Pin YangAbstract:The determination of Inorganic Phosphorus in human urine is very important, since it has diagnostic value in some clinical cases. Here we apply a simple, sensitive and direct method to determine Inorganic Phosphorus in urine. This new ensemble is prepared by adding ytterbium chloride and pyrocatechol violet in a 2:1 molar ratio in an aqueous solution of 10 mM 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid buffer at pH 7.0. The addition of the urine sample turned the blue ensemble yellow and altered the UV-vis absorption spectra. The ensemble exhibits excellent selectivity for Inorganic Phosphorus over other constituents of urine. We validate the accuracy of our method by the standard procedure (molybdenum blue assay for phosphate). The detection results are basically consistent with normal excretion of phosphate. Furthermore, we fabricated a new kind of Inorganic Phosphorus reagent kit, which enables us to inspect phosphate concentrations of urine with the naked eye. Fit for all kinds of various clinic uses, our reagent kit is a hopeful substitute for the molybdenum reagent kit.
Richard J. Wood - One of the best experts on this subject based on the ideXlab platform.
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Inorganic Phosphorus Reduces Hypercalciuria During Total Parenteral Nutrition By Enhancing Renal Tubular Calcium Absorption
JPEN. Journal of parenteral and enteral nutrition, 1998Co-Authors: Charles H. Berkelhammer, Richard J. Wood, Michael D. SitrinAbstract:Background: Increasing the Inorganic Phosphorus content of total parenteral nutrition (TPN) formulas has been shown to decrease TPN-induced hypercalciuria in experimental animals and humans. The mechanism of this effect, however, has been uncertain. Methods: By using a randomized cross-over design, seven patients on cyclic TPN were given otherwise identical formulas providing either 15 or 45 mmol/d of Inorganic Phosphorus. Urinary calcium excretion, serum ultrafilterable calcium, filtered calcium load, fractional calcium excretion, urinary cyclic adenosine 5'-monophosphate (cAMP), and serum levels of ionized calcium, parathyroid hormone (PTH), and vitamin D metabolites were determined at the end of each study period. Results: Urinary calcium excretion was significantly lower when the patients received the higher Inorganic Phosphorus formula. Increasing the Inorganic Phosphorus in the TPN formula did not change ultrafilterable calcium or filtered calcium load, but significantly reduced fractional calcium excretion. No differences in serum levels of ionized calcium, PTH, 25-hydroxyvitamin D, 1,25-dihydroxyvitamin D, or urinary cAMP were observed between treatments. Conclusions: These results demonstrate that increasing the Inorganic Phosphorus content of the TPN formula decreases urinary calcium excretion by increasing renal tubular calcium resorption. This effect is not due to alterations in the PTH-1,25-dihydroxyitamin D axis, but likely reflects a direct action of Inorganic Phosphorus on the renal tubules.