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Margareth Lara Capurro - One of the best experts on this subject based on the ideXlab platform.
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effects of plasmodium gallinaceum on hemolymph physiology of aedes aegypti during parasite development
Journal of Insect Physiology, 2011Co-Authors: Ricardo Vieira Araujo, Ceres Maciel, Klaus Hartfelder, Margareth Lara CapurroAbstract:Insect Disease vectors show diminished fecundity when infected with Plasmodium. This phenomenon has already been demonstrated in laboratory models such as Aedes aegypti, Anopheles gambiae and Anopheles stephensi. This study demonstrates several changes in physiological processes of A. aegypti occurring upon infection with Plasmodium gallinaceum, such as reduced ecdysteroid levels in hemolymph as well as altered expression patterns for genes involved in vitellogenesis, lipid transport and immune response. Furthermore, we could show that P. gallinaceum infected A. aegypti presented a reduction in reproductive fitness, accompanied by an activated innate immune response and increase in lipophorin expression, with the latter possibly representing a nutritional resource for Plasmodium sporozoites.
Sheila Ons - One of the best experts on this subject based on the ideXlab platform.
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Neuropeptide precursor gene discovery in the Chagas Disease vector Rhodnius prolixus.
Insect molecular biology, 2010Co-Authors: Sheila Ons, Marcos Sterkel, Luis Diambra, Henning Urlaub, Rolando Rivera-pomarAbstract:We show a straightforward workflow combining homology search in Rhodnius prolixus genome sequence with cloning by rapid amplification of cDNA ends and mass spectrometry. We have identified 32 genes and their transcripts that encode a number of neuropeptide precursors leading to 194 putative peptides. We validated by mass spectrometry 82 of those predicted neuropeptides in the brain of R. prolixus to achieve the first comprehensive genomic, transcriptomic and neuropeptidomic analysis of an Insect Disease vector. Comparisons of available Insect neuropeptide sequences revealed that the R. prolixus genome contains most of the conserved neuropeptides in Insects, many of them displaying specific features at the sequence level. Some gene families reported here are identified for the first time in the order Hemiptera, a highly biodiverse group of Insects that includes many human, animal and plant Disease agents.
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The neuropeptidome of Rhodnius prolixus brain
Proteomics, 2009Co-Authors: Sheila Ons, Henning Urlaub, Florian Richter, Rolando Rivera PomarAbstract:We show a sensitive and straightforward off-line nano-LC-MALDI-MS/MS workflow that allowed the first comprehensive neuropeptidomic analysis of an Insect Disease vector. This approach was applied to identify neuropeptides in the brain of Rhodnius prolixus, a vector of Chagas Disease. This work will contribute to the annotation of genes in the ongoing R. prolixus genome sequence project. Peptides were identified by de novo sequencing and comparisons to known neuropeptides from different organisms by database search. By these means, we were able to identify 42 novel neuropeptides from R. prolixus. The peptides were classified as extended FMRF-amide-related peptides, sulfakinins, myosuppressins, short neuropeptide F, long neuropeptide F, SIF-amide-related peptides, tachykinins, orcokinins, allatostatins, allatotropins, calcitonin-like diuretic hormones, corazonin, and pyrokinin. Some of them were detected in multiple isoforms and/or truncated fragments. Interestingly, some of the R. prolixus peptides, as myosuppressin and sulfakinins, are unique in their characteristic C-terminal domain among Insect neuropeptides identified so far.
Mar Cabeza - One of the best experts on this subject based on the ideXlab platform.
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Bats as potential suppressors of multiple agricultural pests: A case study from Madagascar
Agriculture Ecosystems & Environment, 2019Co-Authors: James Kemp, Adrià López-baucells, Ricardo Rocha, Owen S. Wangensteen, Zo Emmanuel Andriatafika, Abhilash Nair, Mar CabezaAbstract:Abstract The conversion of natural habitats to agriculture is one of the main drivers of biotic change. Madagascar is no exception and land-use change, mostly driven by slash-and-burn agriculture, is impacting the island's exceptional biodiversity. Although most species are negatively affected by agricultural expansion, some, such as synanthropic bats, are capable of exploring newly available resources and benefit from man-made agricultural ecosystems. As bats are known predators of agricultural pests it seems possible that Malagasy bats may be preferentially foraging within agricultural areas and therefore provide important pest suppression services. To investigate the potential role of bats as pest suppressors, we conducted acoustic surveys of Insectivorous bats in and around Ranomafana National Park, Madagascar, during November and December 2015. We surveyed five landcover types: irrigated rice, hillside rice, secondary vegetation, forest fragment and continuous forest. 9569 bat passes from a regional assemblage of 19 species were recorded. In parallel, we collected faeces from the six most common bat species to detect Insect pest species in their diet using DNA metabarcoding. Total bat activity was higher over rice fields when compared to forest and bats belonging to the open space and edge space sonotypes were the most benefited by the conversion of forest to hillside and irrigated rice. Two economically important rice pests were detected in the faecal samples collected - the paddy swarming armyworm Spodoptera mauritia was detected in Mops leucogaster samples while the grass webworm Herpetogramma licarsisalis was detected from Mormopterus jugularis and Miniopterus majori samples. Other crops pests detected included the sugarcane cicada Yanga guttulata, the macadamia nut-borer Thaumatotibia batrachopa and the sober tabby Ericeia inangulata (a pest of citrus fruits). Samples from all bat species also contained reads from important Insect Disease vectors. In light of our results we argue that Malagasy Insectivorous bats have the potential to suppress agricultural pests. It is important to retain and maximise Malagasy bat populations as they may contribute to higher agricultural yields and promote sustainable livelihoods.
Ricardo Vieira Araujo - One of the best experts on this subject based on the ideXlab platform.
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effects of plasmodium gallinaceum on hemolymph physiology of aedes aegypti during parasite development
Journal of Insect Physiology, 2011Co-Authors: Ricardo Vieira Araujo, Ceres Maciel, Klaus Hartfelder, Margareth Lara CapurroAbstract:Insect Disease vectors show diminished fecundity when infected with Plasmodium. This phenomenon has already been demonstrated in laboratory models such as Aedes aegypti, Anopheles gambiae and Anopheles stephensi. This study demonstrates several changes in physiological processes of A. aegypti occurring upon infection with Plasmodium gallinaceum, such as reduced ecdysteroid levels in hemolymph as well as altered expression patterns for genes involved in vitellogenesis, lipid transport and immune response. Furthermore, we could show that P. gallinaceum infected A. aegypti presented a reduction in reproductive fitness, accompanied by an activated innate immune response and increase in lipophorin expression, with the latter possibly representing a nutritional resource for Plasmodium sporozoites.
Henning Urlaub - One of the best experts on this subject based on the ideXlab platform.
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Neuropeptide precursor gene discovery in the Chagas Disease vector Rhodnius prolixus.
Insect molecular biology, 2010Co-Authors: Sheila Ons, Marcos Sterkel, Luis Diambra, Henning Urlaub, Rolando Rivera-pomarAbstract:We show a straightforward workflow combining homology search in Rhodnius prolixus genome sequence with cloning by rapid amplification of cDNA ends and mass spectrometry. We have identified 32 genes and their transcripts that encode a number of neuropeptide precursors leading to 194 putative peptides. We validated by mass spectrometry 82 of those predicted neuropeptides in the brain of R. prolixus to achieve the first comprehensive genomic, transcriptomic and neuropeptidomic analysis of an Insect Disease vector. Comparisons of available Insect neuropeptide sequences revealed that the R. prolixus genome contains most of the conserved neuropeptides in Insects, many of them displaying specific features at the sequence level. Some gene families reported here are identified for the first time in the order Hemiptera, a highly biodiverse group of Insects that includes many human, animal and plant Disease agents.
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The neuropeptidome of Rhodnius prolixus brain
Proteomics, 2009Co-Authors: Sheila Ons, Henning Urlaub, Florian Richter, Rolando Rivera PomarAbstract:We show a sensitive and straightforward off-line nano-LC-MALDI-MS/MS workflow that allowed the first comprehensive neuropeptidomic analysis of an Insect Disease vector. This approach was applied to identify neuropeptides in the brain of Rhodnius prolixus, a vector of Chagas Disease. This work will contribute to the annotation of genes in the ongoing R. prolixus genome sequence project. Peptides were identified by de novo sequencing and comparisons to known neuropeptides from different organisms by database search. By these means, we were able to identify 42 novel neuropeptides from R. prolixus. The peptides were classified as extended FMRF-amide-related peptides, sulfakinins, myosuppressins, short neuropeptide F, long neuropeptide F, SIF-amide-related peptides, tachykinins, orcokinins, allatostatins, allatotropins, calcitonin-like diuretic hormones, corazonin, and pyrokinin. Some of them were detected in multiple isoforms and/or truncated fragments. Interestingly, some of the R. prolixus peptides, as myosuppressin and sulfakinins, are unique in their characteristic C-terminal domain among Insect neuropeptides identified so far.