The Experts below are selected from a list of 258 Experts worldwide ranked by ideXlab platform
Peter Roepstorff - One of the best experts on this subject based on the ideXlab platform.
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isoforms of a cuticular protein from Larvae of the meal beetle tenebrio molitor studied by mass spectrometry in combination with edman degradation and two dimensional polyacrylamide gel electrophoresis
Protein Science, 2008Co-Authors: Sophie Haebel, Charlotte Harken Jensen, Svend Olav Andersen, Peter RoepstorffAbstract:Simultaneous sequencing, using a combination of mass spectrometry and Edman degradation, of three approximately 15-kDa variants of a cuticular protein extracted from the meal beetle Tenebrio molitor Larva is demonstrated. The information obtained by matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) time-course monitoring of enzymatic digests was found essential to identify the differences among the three variants and for alignment of the peptides in the sequence. To determine whether each individual Insect Larva contains all three protein variants, proteins extracted from single animals were separated by two-dimensional gel electrophoresis, electroeluted from the gel spots, and analyzed by MALDI MS. Molecular weights of the proteins present in each sample could be obtained, and mass spectrometric mapping of the peptides after digestion with trypsin gave additional information. The protein isoforms were found to be allelic variants.
Oliver Betz - One of the best experts on this subject based on the ideXlab platform.
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an Insect Larva with a pig snout structure and function of the nasale of hyphydrus ovatus l 1763 coleoptera dytiscidae
Journal of Zoology, 2003Co-Authors: Henning Friis, Thomas Bauer, Oliver BetzAbstract:Hyphydrus ovatus beetles inhabit lakes, ponds and slow running streams. In central Europe the Larvae develop at water temperatures of >10°C from May to July. All three instars live on or close to plants. The first instar prefers the upper areas of plants. Its prey are Insect Larvae and small stages of planktonic crustaceans. The second instar chooses deeper areas and catches larger prey, whereas the third instar prefers the root zone and feeds mainly on tubificids and chironomids that are caught in the detritus of the bottom layer. The elongated nasale of Hyphydrus ovatus is equipped with different sensilla. Despite the rather different shape of their outer parts, their ultrastructure indicates that they all have a mechanoreceptive function. Chaetiform sensilla are especially numerous and are especially long near the tip. Plate-like mechanosensilla are positioned in a half circle at the ventral edge of the rostral tip. The assumed function of these sensilla is the location of prey. As in other Hydroporinae, the axis connecting the dorsal and ventral points of articulation of the mandibles is sloped laterally. Thereby, the closing mandibles move upwards and press the prey against the underside of the nasale, which – like the upper jaw in vertebrates – serves as the counterpart of the mandibles. On its ventral side, the nasale is equipped with various cuticular teeth that serve to hold the prey when it is pressed against them by the mandibles.
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An Insect Larva with a ‘pig-snout’: structure and function of the nasale of Hyphydrus ovatus L. (1763) (Coleoptera: Dytiscidae)
Journal of Zoology, 2003Co-Authors: Henning Friis, Thomas Bauer, Oliver BetzAbstract:Hyphydrus ovatus beetles inhabit lakes, ponds and slow running streams. In central Europe the Larvae develop at water temperatures of >10°C from May to July. All three instars live on or close to plants. The first instar prefers the upper areas of plants. Its prey are Insect Larvae and small stages of planktonic crustaceans. The second instar chooses deeper areas and catches larger prey, whereas the third instar prefers the root zone and feeds mainly on tubificids and chironomids that are caught in the detritus of the bottom layer. The elongated nasale of Hyphydrus ovatus is equipped with different sensilla. Despite the rather different shape of their outer parts, their ultrastructure indicates that they all have a mechanoreceptive function. Chaetiform sensilla are especially numerous and are especially long near the tip. Plate-like mechanosensilla are positioned in a half circle at the ventral edge of the rostral tip. The assumed function of these sensilla is the location of prey. As in other Hydroporinae, the axis connecting the dorsal and ventral points of articulation of the mandibles is sloped laterally. Thereby, the closing mandibles move upwards and press the prey against the underside of the nasale, which – like the upper jaw in vertebrates – serves as the counterpart of the mandibles. On its ventral side, the nasale is equipped with various cuticular teeth that serve to hold the prey when it is pressed against them by the mandibles.
Sophie Haebel - One of the best experts on this subject based on the ideXlab platform.
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isoforms of a cuticular protein from Larvae of the meal beetle tenebrio molitor studied by mass spectrometry in combination with edman degradation and two dimensional polyacrylamide gel electrophoresis
Protein Science, 2008Co-Authors: Sophie Haebel, Charlotte Harken Jensen, Svend Olav Andersen, Peter RoepstorffAbstract:Simultaneous sequencing, using a combination of mass spectrometry and Edman degradation, of three approximately 15-kDa variants of a cuticular protein extracted from the meal beetle Tenebrio molitor Larva is demonstrated. The information obtained by matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) time-course monitoring of enzymatic digests was found essential to identify the differences among the three variants and for alignment of the peptides in the sequence. To determine whether each individual Insect Larva contains all three protein variants, proteins extracted from single animals were separated by two-dimensional gel electrophoresis, electroeluted from the gel spots, and analyzed by MALDI MS. Molecular weights of the proteins present in each sample could be obtained, and mass spectrometric mapping of the peptides after digestion with trypsin gave additional information. The protein isoforms were found to be allelic variants.
William E. Bentley - One of the best experts on this subject based on the ideXlab platform.
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Short communication Rapid non-invasive monitoring of baculovirus infection for Insect Larvae using green fluorescent protein reporter under early-to-late promoter and a GFP-specific optical probe
2020Co-Authors: Nimish G. Dalal, Shannon F. Kramer, Yordan Kostov, William E. BentleyAbstract:We investigatedmonitoring forbaculovirusinfectionofInsectTrichoplusianiLarvaebycombiningthegreenfluorescent protein(GFP)reporter, the early-to-late (ETL) baculoviral promoter, and a GFP-specific optical probe. Because GFP is under the ETL promoter control, it facilitated rapid monitoring of recombinant baculovirus infection on Insect Larva system. Employment of GFP-specific optical probe also enabled non-invasive and accurate GFP monitoring with 10 h prior to invasive and GFP Western blot assay. This combination may assist in monitoring protein production runs, determining optimal infection and harvest timing, and in general, help to increase the yield of recombinant proteins expressed in Larvae. # 2005 Elsevier Ltd. All rights reserved.
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Rapid non-invasive monitoring of baculovirus infection for Insect Larvae using green fluorescent protein reporter under early-to-late promoter and a GFP-specific optical probe
Process Biochemistry, 2006Co-Authors: Nimish G. Dalal, Shannon F. Kramer, Yordan Kostov, William E. BentleyAbstract:We investigated monitoring for baculovirus infection of Insect Trichoplusia ni Larvae by combining the green fluorescent protein (GFP) reporter, the early-to-late (ETL) baculoviral promoter, and a GFP-specific optical probe. Because GFP is under the ETL promoter control, it facilitated rapid monitoring of recombinant baculovirus infection on Insect Larva system. Employment of GFP-specific optical probe also enabled non-invasive and accurate GFP monitoring with ∼10 h prior to invasive and GFP Western blot assay. This combination may assist in monitoring protein production runs, determining optimal infection and harvest timing, and in general, help to increase the yield of recombinant proteins expressed in Larvae.
Henning Friis - One of the best experts on this subject based on the ideXlab platform.
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an Insect Larva with a pig snout structure and function of the nasale of hyphydrus ovatus l 1763 coleoptera dytiscidae
Journal of Zoology, 2003Co-Authors: Henning Friis, Thomas Bauer, Oliver BetzAbstract:Hyphydrus ovatus beetles inhabit lakes, ponds and slow running streams. In central Europe the Larvae develop at water temperatures of >10°C from May to July. All three instars live on or close to plants. The first instar prefers the upper areas of plants. Its prey are Insect Larvae and small stages of planktonic crustaceans. The second instar chooses deeper areas and catches larger prey, whereas the third instar prefers the root zone and feeds mainly on tubificids and chironomids that are caught in the detritus of the bottom layer. The elongated nasale of Hyphydrus ovatus is equipped with different sensilla. Despite the rather different shape of their outer parts, their ultrastructure indicates that they all have a mechanoreceptive function. Chaetiform sensilla are especially numerous and are especially long near the tip. Plate-like mechanosensilla are positioned in a half circle at the ventral edge of the rostral tip. The assumed function of these sensilla is the location of prey. As in other Hydroporinae, the axis connecting the dorsal and ventral points of articulation of the mandibles is sloped laterally. Thereby, the closing mandibles move upwards and press the prey against the underside of the nasale, which – like the upper jaw in vertebrates – serves as the counterpart of the mandibles. On its ventral side, the nasale is equipped with various cuticular teeth that serve to hold the prey when it is pressed against them by the mandibles.
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An Insect Larva with a ‘pig-snout’: structure and function of the nasale of Hyphydrus ovatus L. (1763) (Coleoptera: Dytiscidae)
Journal of Zoology, 2003Co-Authors: Henning Friis, Thomas Bauer, Oliver BetzAbstract:Hyphydrus ovatus beetles inhabit lakes, ponds and slow running streams. In central Europe the Larvae develop at water temperatures of >10°C from May to July. All three instars live on or close to plants. The first instar prefers the upper areas of plants. Its prey are Insect Larvae and small stages of planktonic crustaceans. The second instar chooses deeper areas and catches larger prey, whereas the third instar prefers the root zone and feeds mainly on tubificids and chironomids that are caught in the detritus of the bottom layer. The elongated nasale of Hyphydrus ovatus is equipped with different sensilla. Despite the rather different shape of their outer parts, their ultrastructure indicates that they all have a mechanoreceptive function. Chaetiform sensilla are especially numerous and are especially long near the tip. Plate-like mechanosensilla are positioned in a half circle at the ventral edge of the rostral tip. The assumed function of these sensilla is the location of prey. As in other Hydroporinae, the axis connecting the dorsal and ventral points of articulation of the mandibles is sloped laterally. Thereby, the closing mandibles move upwards and press the prey against the underside of the nasale, which – like the upper jaw in vertebrates – serves as the counterpart of the mandibles. On its ventral side, the nasale is equipped with various cuticular teeth that serve to hold the prey when it is pressed against them by the mandibles.