The Experts below are selected from a list of 66 Experts worldwide ranked by ideXlab platform
György Csaba - One of the best experts on this subject based on the ideXlab platform.
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Effect of glucose on the Insulin production and Insulin Binding of Tetrahymena.
Acta microbiologica et immunologica Hungarica, 2012Co-Authors: Eszter Lajkó, Éva Pállinger, György CsabaAbstract:As the unicellular ciliate, Tetrahymena has Insulin receptors and produces Insulin itself, which can regulate its glucose metabolism and other cell functions, in the present experiments the feed-back, the effect of glucose on the Insulin Binding and Insulin production was studied. The cells were kept partly in tryptone-yeast medium, partly in Losina salt solution. The duration of treatment (in 0.1, 1.0, 10.0 mg/ml glucose) in the Binding study was 10 min, in the hormone production study 30 min. FITC-Insulin Binding was significantly decreased only by 0.1 mg/ml glucose treatment in medium and by 10 mg/ml glucose in salt. The Insulin production was significantly lower only in cells treated with 10 mg/ml glucose in medium. The Insulin Binding in salt was always higher and the Insulin production always lower, than in medium. Earlier results demonstrated that the hormonal system (presence of hormones, receptors and signal pathways) of higher ranked animals can be deduced to a unicellular level, however, the fe...
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Effect of starvation on Insulin production and Insulin Binding in Tetrahymena.
Cell biochemistry and function, 2007Co-Authors: György Csaba, Péter Kovács, Éva PállingerAbstract:Tetrahymena pyriformis GL was starved for 24 h and then the immunologically demonstrable Insulin content and FITC-Insulin Binding were measured by flow cytometry and localization was studied by confocal microscopy. The amount of endogeneous Insulin as well as FITC Insulin Binding, was highly significantly elevated. Glucose feeding for 30 min abolished the elevation of FITC-Insulin Binding. In starved cells, Insulin-Binding sites disappeared from the surface and FITC-Insulin was bound inside the cells, within large food vacuoles. Endogeneous Insulin was dispersed in the cytoplasm both in the control and starved cells and food vacuoles did not contain it. The results call attention to the stimulatory effect of starvation on Insulin production in Tetrahymena, in parallel with the internal storage of Insulin receptors, which points to an autocrine mechanism.
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Effect of the tyrosine kinase inhibitor tyrphostin on Insulin Binding and Insulin imprinting of Tetrahymena.
Microbios, 1994Co-Authors: Péter Kovács, György CsabaAbstract:After 10 min treatment with the tyrosine kinase inhibitor tyrphostin the fluorescein isothiocyanate (FITC)-Insulin Binding of Tetrahymena was increased. This Binding characteristic was not changed when tyrphostin and Insulin acted together. Consecutive treatments with 10 min tyrphostin and 10 min Insulin further increased the FITC-Insulin Binding. Due to the effect of treatment with tyrphostin there was a decreased Insulin Binding in the offspring generations after 24 h. There was increased Insulin Binding in those cells of which the progenitor was treated not only by tyrphostin but also with Insulin, but the Insulin Binding was still below the control level in this group. The increase of Binding in the offspring generations of cells which were treated consecutively by tyrphostin and Insulin compared with cells treated with only tyrphostin was not less than the increase of Binding in the offspring of the untreated and Insulin pretreated (24 h before) cells. This meant that tyrphostin had the potency to decrease Insulin Binding in the offspring generations but the development of imprinting was not influenced.
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Correlation of Insulin pretreatment and Insulin Binding of Amoeba proteus—a new technique for evaluation
Comparative Biochemistry and Physiology Part A: Physiology, 1992Co-Authors: László Köhidai, A Kihara, György CsabaAbstract:Abstract 1. 1. Insulin Binding sites of Amoeba proteus were analysed. The developed technique helped to compare equivalent pseudopodia membranes, according to their fluorescein-isothiocyanate (FITC)-Insulin Binding capacity. 2. 2. The number of Insulin Binding sites decrease in the control conditions as a fraction of time. 3. 3. The down regulation of Binding sites (or similar phenomenon) lasts ~48 hr. 4. 4. Only low concentrations of Insulin can induce a Binding site elevation in the long term. 5. 5. Hormonal imprinting could be presented in Amoeba . 6. 6. The developed technique is suitable for detection of membrane dynamics in Amoeba proteus .
Éva Pállinger - One of the best experts on this subject based on the ideXlab platform.
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Effect of glucose on the Insulin production and Insulin Binding of Tetrahymena.
Acta microbiologica et immunologica Hungarica, 2012Co-Authors: Eszter Lajkó, Éva Pállinger, György CsabaAbstract:As the unicellular ciliate, Tetrahymena has Insulin receptors and produces Insulin itself, which can regulate its glucose metabolism and other cell functions, in the present experiments the feed-back, the effect of glucose on the Insulin Binding and Insulin production was studied. The cells were kept partly in tryptone-yeast medium, partly in Losina salt solution. The duration of treatment (in 0.1, 1.0, 10.0 mg/ml glucose) in the Binding study was 10 min, in the hormone production study 30 min. FITC-Insulin Binding was significantly decreased only by 0.1 mg/ml glucose treatment in medium and by 10 mg/ml glucose in salt. The Insulin production was significantly lower only in cells treated with 10 mg/ml glucose in medium. The Insulin Binding in salt was always higher and the Insulin production always lower, than in medium. Earlier results demonstrated that the hormonal system (presence of hormones, receptors and signal pathways) of higher ranked animals can be deduced to a unicellular level, however, the fe...
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Effect of starvation on Insulin production and Insulin Binding in Tetrahymena.
Cell biochemistry and function, 2007Co-Authors: György Csaba, Péter Kovács, Éva PállingerAbstract:Tetrahymena pyriformis GL was starved for 24 h and then the immunologically demonstrable Insulin content and FITC-Insulin Binding were measured by flow cytometry and localization was studied by confocal microscopy. The amount of endogeneous Insulin as well as FITC Insulin Binding, was highly significantly elevated. Glucose feeding for 30 min abolished the elevation of FITC-Insulin Binding. In starved cells, Insulin-Binding sites disappeared from the surface and FITC-Insulin was bound inside the cells, within large food vacuoles. Endogeneous Insulin was dispersed in the cytoplasm both in the control and starved cells and food vacuoles did not contain it. The results call attention to the stimulatory effect of starvation on Insulin production in Tetrahymena, in parallel with the internal storage of Insulin receptors, which points to an autocrine mechanism.
Péter Kovács - One of the best experts on this subject based on the ideXlab platform.
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Effect of starvation on Insulin production and Insulin Binding in Tetrahymena.
Cell biochemistry and function, 2007Co-Authors: György Csaba, Péter Kovács, Éva PállingerAbstract:Tetrahymena pyriformis GL was starved for 24 h and then the immunologically demonstrable Insulin content and FITC-Insulin Binding were measured by flow cytometry and localization was studied by confocal microscopy. The amount of endogeneous Insulin as well as FITC Insulin Binding, was highly significantly elevated. Glucose feeding for 30 min abolished the elevation of FITC-Insulin Binding. In starved cells, Insulin-Binding sites disappeared from the surface and FITC-Insulin was bound inside the cells, within large food vacuoles. Endogeneous Insulin was dispersed in the cytoplasm both in the control and starved cells and food vacuoles did not contain it. The results call attention to the stimulatory effect of starvation on Insulin production in Tetrahymena, in parallel with the internal storage of Insulin receptors, which points to an autocrine mechanism.
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Effect of the tyrosine kinase inhibitor tyrphostin on Insulin Binding and Insulin imprinting of Tetrahymena.
Microbios, 1994Co-Authors: Péter Kovács, György CsabaAbstract:After 10 min treatment with the tyrosine kinase inhibitor tyrphostin the fluorescein isothiocyanate (FITC)-Insulin Binding of Tetrahymena was increased. This Binding characteristic was not changed when tyrphostin and Insulin acted together. Consecutive treatments with 10 min tyrphostin and 10 min Insulin further increased the FITC-Insulin Binding. Due to the effect of treatment with tyrphostin there was a decreased Insulin Binding in the offspring generations after 24 h. There was increased Insulin Binding in those cells of which the progenitor was treated not only by tyrphostin but also with Insulin, but the Insulin Binding was still below the control level in this group. The increase of Binding in the offspring generations of cells which were treated consecutively by tyrphostin and Insulin compared with cells treated with only tyrphostin was not less than the increase of Binding in the offspring of the untreated and Insulin pretreated (24 h before) cells. This meant that tyrphostin had the potency to decrease Insulin Binding in the offspring generations but the development of imprinting was not influenced.
Michael T. Clandinin - One of the best experts on this subject based on the ideXlab platform.
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dietary omega 3 and polyunsaturated fatty acids modify fatty acyl composition and Insulin Binding in skeletal muscle sarcolemma
Biochemical Journal, 1994Co-Authors: Vickie E Baracos, H A Quinney, Michael T. ClandininAbstract:: Feeding animals with diets high in saturated fat induces Insulin resistance, and replacing saturated fat isocalorically with poly-unsaturated fat, especially long-chain omega-3 fatty acids, will prevent the development of Insulin resistance in skeletal-muscle tissue. To investigate the mechanism, rats were fed on high-fat (20%, w/w) semipurified diets for 6 weeks. Diets containing ratios of polyunsaturated/saturated (P/S) fatty acid of 0.25 (low-P/S diet) and 1.0 (high-P/S diet) were used to study the effect of the level of saturated fat. To study the effects of omega-3 fatty acids, diets with a low-P/S ratio containing either 0 (low-omega-3 diet) or 3.3% (high-omega-3 diet) long-chain omega-3 fatty acids from fish oil were fed. Plasma membrane from skeletal muscle was purified. The content of fatty acids in sarcolemmal phospholipid was significantly related to the dietary composition. Insulin Binding to intact sarcolemmal vesicles prepared from rats fed on diets high in omega-3 fatty acids increased 14-fold compared with animals fed on the low-omega-3 diet (P < 0.0001). Feeding rats on a diet with a high P/S ratio increased sarcolemmal Insulin Binding by 2.3-fold (P < 0.05). Increased Insulin Binding was due to increased receptor number at the low-affinity high-capacity Binding site. Dietary effects on Insulin Binding were eliminated when studies were carried out on detergent-solubilized membranes, indicating the importance of the phospholipid fatty acyl composition for Insulin Binding. The results suggest that dietary omega-3 and polyunsaturated fatty acids increase Insulin Binding to sarcolemma by changing the fatty acyl composition of phospholipid surrounding the Insulin receptor, and this might be the mechanism by which dietary fatty acids modify Insulin action.
Vickie E Baracos - One of the best experts on this subject based on the ideXlab platform.
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dietary omega 3 and polyunsaturated fatty acids modify fatty acyl composition and Insulin Binding in skeletal muscle sarcolemma
Biochemical Journal, 1994Co-Authors: Vickie E Baracos, H A Quinney, Michael T. ClandininAbstract:: Feeding animals with diets high in saturated fat induces Insulin resistance, and replacing saturated fat isocalorically with poly-unsaturated fat, especially long-chain omega-3 fatty acids, will prevent the development of Insulin resistance in skeletal-muscle tissue. To investigate the mechanism, rats were fed on high-fat (20%, w/w) semipurified diets for 6 weeks. Diets containing ratios of polyunsaturated/saturated (P/S) fatty acid of 0.25 (low-P/S diet) and 1.0 (high-P/S diet) were used to study the effect of the level of saturated fat. To study the effects of omega-3 fatty acids, diets with a low-P/S ratio containing either 0 (low-omega-3 diet) or 3.3% (high-omega-3 diet) long-chain omega-3 fatty acids from fish oil were fed. Plasma membrane from skeletal muscle was purified. The content of fatty acids in sarcolemmal phospholipid was significantly related to the dietary composition. Insulin Binding to intact sarcolemmal vesicles prepared from rats fed on diets high in omega-3 fatty acids increased 14-fold compared with animals fed on the low-omega-3 diet (P < 0.0001). Feeding rats on a diet with a high P/S ratio increased sarcolemmal Insulin Binding by 2.3-fold (P < 0.05). Increased Insulin Binding was due to increased receptor number at the low-affinity high-capacity Binding site. Dietary effects on Insulin Binding were eliminated when studies were carried out on detergent-solubilized membranes, indicating the importance of the phospholipid fatty acyl composition for Insulin Binding. The results suggest that dietary omega-3 and polyunsaturated fatty acids increase Insulin Binding to sarcolemma by changing the fatty acyl composition of phospholipid surrounding the Insulin receptor, and this might be the mechanism by which dietary fatty acids modify Insulin action.