The Experts below are selected from a list of 12786 Experts worldwide ranked by ideXlab platform

Akihiko Kondo - One of the best experts on this subject based on the ideXlab platform.

  • mapping of endoglucanases displayed on yeast cell surface using atomic Force microscopy
    Colloids and Surfaces B: Biointerfaces, 2017
    Co-Authors: Musashi Takenaka, Tomohisa Hasunuma, Chiaki Ogino, Takuya Kobayashi, Kentaro Inokuma, Tatsuo Maruyama, Akihiko Kondo
    Abstract:

    The surface of yeast cells has been an attractive interface for the effective use of cellulose. Surface enzymes, however, are difficult to visualize and evaluate. In this study, two kinds of unique anchoring regions were used to display the cellulase, endoglucanase (EG), on a yeast cell surface. Differences in the display level and the localization of EG were observed by atomic Force microscopy. By surveying the yeast cell surface with a chemically modified cantilever, the Interactive Force between the cellulose and EG was measured. Force curve mapping revealed differences in the display levels and the localization of EG according to anchoring regions. The proposed methodology enables visualization of displayed enzymes such as EG on the yeast cell surface.

  • dna duplex linker for afm selex of dna aptamer against human serum albumin
    Bioorganic & Medicinal Chemistry Letters, 2017
    Co-Authors: Musashi Takenaka, Yuzo Okumura, Tomokazu Amino, Yusuke Miyachi, Chiaki Ogino, Akihiko Kondo
    Abstract:

    DNA-duplex interactions in thymines and adenins are used as a linker for the novel methodology of Atomic Force Microscope-Systematic Evolution of Ligands by EXpotential enrichment (AFM-SELEX). This study used the hydrogen bonds in 10 mer of both thymines (T10) and adenines (A10). Initially, the Interactive Force in T10-A10 was measured by AFM, which returned an average Interactive Force of approximately 350pN. Based on this result, DNA aptamers against human serum albumin could be selected in the 4th round, and 15 different clones could be sequenced. The lowest dissociation constant of the selected aptamer was identified via surface plasmon resonance, and it proved to be identical to that of the commercial aptamer. Therefore, specific hydrogen bonds in DNA can be useful linkers for AFM-SELEX.

  • mapping of endoglucanases displayed on yeast cell surface using atomic Force microscopy b biointerfaces
    Colloids and Surfaces, 2017
    Co-Authors: Musashi Takenaka, Tomohisa Hasunuma, Chiaki Ogino, Takuya Kobayashi, Kentaro Inokuma, Tatsuo Maruyama, Akihiko Kondo
    Abstract:

    The surface of yeast cells has been an attractive interface for the effective use of cellulose. Surface enzymes, however, are difficult to visualize and evaluate. In this study, two kinds of unique anchoring regions were used to display the cellulase, endoglucanase (EG), on a yeast cell surface. Differences in the display level and the localization of EG were observed by atomic Force microscopy. By surveying the yeast cell surface with a chemically modified cantilever, the Interactive Force between the cellulose and EG was measured. Force curve mapping revealed differences in the display levels and the localization of EG according to anchoring regions. The proposed methodology enables visualization of displayed enzymes such as EG on the yeast cell surface.

Musashi Takenaka - One of the best experts on this subject based on the ideXlab platform.

  • mapping of endoglucanases displayed on yeast cell surface using atomic Force microscopy
    Colloids and Surfaces B: Biointerfaces, 2017
    Co-Authors: Musashi Takenaka, Tomohisa Hasunuma, Chiaki Ogino, Takuya Kobayashi, Kentaro Inokuma, Tatsuo Maruyama, Akihiko Kondo
    Abstract:

    The surface of yeast cells has been an attractive interface for the effective use of cellulose. Surface enzymes, however, are difficult to visualize and evaluate. In this study, two kinds of unique anchoring regions were used to display the cellulase, endoglucanase (EG), on a yeast cell surface. Differences in the display level and the localization of EG were observed by atomic Force microscopy. By surveying the yeast cell surface with a chemically modified cantilever, the Interactive Force between the cellulose and EG was measured. Force curve mapping revealed differences in the display levels and the localization of EG according to anchoring regions. The proposed methodology enables visualization of displayed enzymes such as EG on the yeast cell surface.

  • dna duplex linker for afm selex of dna aptamer against human serum albumin
    Bioorganic & Medicinal Chemistry Letters, 2017
    Co-Authors: Musashi Takenaka, Yuzo Okumura, Tomokazu Amino, Yusuke Miyachi, Chiaki Ogino, Akihiko Kondo
    Abstract:

    DNA-duplex interactions in thymines and adenins are used as a linker for the novel methodology of Atomic Force Microscope-Systematic Evolution of Ligands by EXpotential enrichment (AFM-SELEX). This study used the hydrogen bonds in 10 mer of both thymines (T10) and adenines (A10). Initially, the Interactive Force in T10-A10 was measured by AFM, which returned an average Interactive Force of approximately 350pN. Based on this result, DNA aptamers against human serum albumin could be selected in the 4th round, and 15 different clones could be sequenced. The lowest dissociation constant of the selected aptamer was identified via surface plasmon resonance, and it proved to be identical to that of the commercial aptamer. Therefore, specific hydrogen bonds in DNA can be useful linkers for AFM-SELEX.

  • mapping of endoglucanases displayed on yeast cell surface using atomic Force microscopy b biointerfaces
    Colloids and Surfaces, 2017
    Co-Authors: Musashi Takenaka, Tomohisa Hasunuma, Chiaki Ogino, Takuya Kobayashi, Kentaro Inokuma, Tatsuo Maruyama, Akihiko Kondo
    Abstract:

    The surface of yeast cells has been an attractive interface for the effective use of cellulose. Surface enzymes, however, are difficult to visualize and evaluate. In this study, two kinds of unique anchoring regions were used to display the cellulase, endoglucanase (EG), on a yeast cell surface. Differences in the display level and the localization of EG were observed by atomic Force microscopy. By surveying the yeast cell surface with a chemically modified cantilever, the Interactive Force between the cellulose and EG was measured. Force curve mapping revealed differences in the display levels and the localization of EG according to anchoring regions. The proposed methodology enables visualization of displayed enzymes such as EG on the yeast cell surface.

Chiaki Ogino - One of the best experts on this subject based on the ideXlab platform.

  • mapping of endoglucanases displayed on yeast cell surface using atomic Force microscopy
    Colloids and Surfaces B: Biointerfaces, 2017
    Co-Authors: Musashi Takenaka, Tomohisa Hasunuma, Chiaki Ogino, Takuya Kobayashi, Kentaro Inokuma, Tatsuo Maruyama, Akihiko Kondo
    Abstract:

    The surface of yeast cells has been an attractive interface for the effective use of cellulose. Surface enzymes, however, are difficult to visualize and evaluate. In this study, two kinds of unique anchoring regions were used to display the cellulase, endoglucanase (EG), on a yeast cell surface. Differences in the display level and the localization of EG were observed by atomic Force microscopy. By surveying the yeast cell surface with a chemically modified cantilever, the Interactive Force between the cellulose and EG was measured. Force curve mapping revealed differences in the display levels and the localization of EG according to anchoring regions. The proposed methodology enables visualization of displayed enzymes such as EG on the yeast cell surface.

  • dna duplex linker for afm selex of dna aptamer against human serum albumin
    Bioorganic & Medicinal Chemistry Letters, 2017
    Co-Authors: Musashi Takenaka, Yuzo Okumura, Tomokazu Amino, Yusuke Miyachi, Chiaki Ogino, Akihiko Kondo
    Abstract:

    DNA-duplex interactions in thymines and adenins are used as a linker for the novel methodology of Atomic Force Microscope-Systematic Evolution of Ligands by EXpotential enrichment (AFM-SELEX). This study used the hydrogen bonds in 10 mer of both thymines (T10) and adenines (A10). Initially, the Interactive Force in T10-A10 was measured by AFM, which returned an average Interactive Force of approximately 350pN. Based on this result, DNA aptamers against human serum albumin could be selected in the 4th round, and 15 different clones could be sequenced. The lowest dissociation constant of the selected aptamer was identified via surface plasmon resonance, and it proved to be identical to that of the commercial aptamer. Therefore, specific hydrogen bonds in DNA can be useful linkers for AFM-SELEX.

  • mapping of endoglucanases displayed on yeast cell surface using atomic Force microscopy b biointerfaces
    Colloids and Surfaces, 2017
    Co-Authors: Musashi Takenaka, Tomohisa Hasunuma, Chiaki Ogino, Takuya Kobayashi, Kentaro Inokuma, Tatsuo Maruyama, Akihiko Kondo
    Abstract:

    The surface of yeast cells has been an attractive interface for the effective use of cellulose. Surface enzymes, however, are difficult to visualize and evaluate. In this study, two kinds of unique anchoring regions were used to display the cellulase, endoglucanase (EG), on a yeast cell surface. Differences in the display level and the localization of EG were observed by atomic Force microscopy. By surveying the yeast cell surface with a chemically modified cantilever, the Interactive Force between the cellulose and EG was measured. Force curve mapping revealed differences in the display levels and the localization of EG according to anchoring regions. The proposed methodology enables visualization of displayed enzymes such as EG on the yeast cell surface.

Takuya Kobayashi - One of the best experts on this subject based on the ideXlab platform.

  • mapping of endoglucanases displayed on yeast cell surface using atomic Force microscopy
    Colloids and Surfaces B: Biointerfaces, 2017
    Co-Authors: Musashi Takenaka, Tomohisa Hasunuma, Chiaki Ogino, Takuya Kobayashi, Kentaro Inokuma, Tatsuo Maruyama, Akihiko Kondo
    Abstract:

    The surface of yeast cells has been an attractive interface for the effective use of cellulose. Surface enzymes, however, are difficult to visualize and evaluate. In this study, two kinds of unique anchoring regions were used to display the cellulase, endoglucanase (EG), on a yeast cell surface. Differences in the display level and the localization of EG were observed by atomic Force microscopy. By surveying the yeast cell surface with a chemically modified cantilever, the Interactive Force between the cellulose and EG was measured. Force curve mapping revealed differences in the display levels and the localization of EG according to anchoring regions. The proposed methodology enables visualization of displayed enzymes such as EG on the yeast cell surface.

  • mapping of endoglucanases displayed on yeast cell surface using atomic Force microscopy b biointerfaces
    Colloids and Surfaces, 2017
    Co-Authors: Musashi Takenaka, Tomohisa Hasunuma, Chiaki Ogino, Takuya Kobayashi, Kentaro Inokuma, Tatsuo Maruyama, Akihiko Kondo
    Abstract:

    The surface of yeast cells has been an attractive interface for the effective use of cellulose. Surface enzymes, however, are difficult to visualize and evaluate. In this study, two kinds of unique anchoring regions were used to display the cellulase, endoglucanase (EG), on a yeast cell surface. Differences in the display level and the localization of EG were observed by atomic Force microscopy. By surveying the yeast cell surface with a chemically modified cantilever, the Interactive Force between the cellulose and EG was measured. Force curve mapping revealed differences in the display levels and the localization of EG according to anchoring regions. The proposed methodology enables visualization of displayed enzymes such as EG on the yeast cell surface.

Tomohisa Hasunuma - One of the best experts on this subject based on the ideXlab platform.

  • mapping of endoglucanases displayed on yeast cell surface using atomic Force microscopy
    Colloids and Surfaces B: Biointerfaces, 2017
    Co-Authors: Musashi Takenaka, Tomohisa Hasunuma, Chiaki Ogino, Takuya Kobayashi, Kentaro Inokuma, Tatsuo Maruyama, Akihiko Kondo
    Abstract:

    The surface of yeast cells has been an attractive interface for the effective use of cellulose. Surface enzymes, however, are difficult to visualize and evaluate. In this study, two kinds of unique anchoring regions were used to display the cellulase, endoglucanase (EG), on a yeast cell surface. Differences in the display level and the localization of EG were observed by atomic Force microscopy. By surveying the yeast cell surface with a chemically modified cantilever, the Interactive Force between the cellulose and EG was measured. Force curve mapping revealed differences in the display levels and the localization of EG according to anchoring regions. The proposed methodology enables visualization of displayed enzymes such as EG on the yeast cell surface.

  • mapping of endoglucanases displayed on yeast cell surface using atomic Force microscopy b biointerfaces
    Colloids and Surfaces, 2017
    Co-Authors: Musashi Takenaka, Tomohisa Hasunuma, Chiaki Ogino, Takuya Kobayashi, Kentaro Inokuma, Tatsuo Maruyama, Akihiko Kondo
    Abstract:

    The surface of yeast cells has been an attractive interface for the effective use of cellulose. Surface enzymes, however, are difficult to visualize and evaluate. In this study, two kinds of unique anchoring regions were used to display the cellulase, endoglucanase (EG), on a yeast cell surface. Differences in the display level and the localization of EG were observed by atomic Force microscopy. By surveying the yeast cell surface with a chemically modified cantilever, the Interactive Force between the cellulose and EG was measured. Force curve mapping revealed differences in the display levels and the localization of EG according to anchoring regions. The proposed methodology enables visualization of displayed enzymes such as EG on the yeast cell surface.