The Experts below are selected from a list of 6690 Experts worldwide ranked by ideXlab platform
Emanuel Heitlinger - One of the best experts on this subject based on the ideXlab platform.
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detection and quantification of house mouse eimeria at the species level challenges and solutions for the assessment of coccidia in wildlife
International journal for parasitology. Parasites and wildlife, 2019Co-Authors: Victor Hugo Jarquindiaz, Alice Balard, Jenny Jost, Julia Kraft, Mert Naci Dikmen, Jana Kvicerova, Emanuel HeitlingerAbstract:Abstract Detection and quantification of coccidia in studies of wildlife can be challenging. Therefore, prevalence of coccidia is often not assessed at the Parasite species level in non-livestock animals. Parasite species – specific prevalences are especially important when studying evolutionary questions in wild populations. We tested whether increased host population density increases prevalence of individual Eimeria species at the farm level, as predicted by epidemiological theory. We studied free-living commensal populations of the house mouse (Mus musculus) in Germany, and established a strategy to detect and quantify Eimeria infections. We show that a novel diagnostic primer targeting the apicoplast genome (Ap5) and coprological assessment after flotation provide complementary detection results increasing sensitivity. Genotyping PCRs confirm detection in a subset of samples and cross-validation of different PCR markers does not indicate bias towards a particular Parasite species in genotyping. We were able to detect double infections and to determine the preferred niche of each Parasite species along the distal-proximal axis of the Intestine. Parasite genotyping from tissue samples provides additional indication for the absence of species bias in genotyping amplifications. Three Eimeria species were found infecting house mice at different prevalences: Eimeria ferrisi (16.7%; 95% CI 13.2–20.7), E. falciformis (4.2%; 95% CI 2.6–6.8) and E. vermiformis (1.9%; 95% CI 0.9–3.8). We also find that mice in dense populations are more likely to be infected with E. falciformis and E. ferrisi. We provide methods for the assessment of prevalences of coccidia at the species level in rodent systems. We show and discuss how such data can help to test hypotheses in ecology, evolution and epidemiology on a species level.
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detection and quantification of house mouse eimeria at the species level challenges and solutions for the assessment of coccidia in wildlife
bioRxiv, 2019Co-Authors: Victor Hugo Jarquindiaz, Alice Balard, Jenny Jost, Julia Kraft, Mert Naci Dikmen, Jana Kvicerova, Emanuel HeitlingerAbstract:Detection and quantification of coccidia in studies of wildlife can be challenging. Therefore, the prevalence of coccidia is often not assessed at the Parasite species level in non-livestock animals. Parasite species-specific prevalences are especially important when studying evolutionary questions in wild populations. We tested whether increased host population density increases the prevalence of individual Eimeria species at the farm level, as predicted by epidemiological theory. We studied free-living commensal populations of the house mouse (Mus musculus) in Germany and established a strategy to detect and quantify Eimeria infections. We show that a novel diagnostic primer targeting the apicoplast genome (Ap5) and coprological assessment after flotation provide complementary detection results increasing sensitivity. Genotyping PCRs confirm detection in a subset of samples and cross-validation of different PCR markers does not indicate a bias towards a particular Parasite species in genotyping. We were able to detect double infections and to determine the preferred niche of each Parasite species along the distal-proximal axis of the Intestine. Parasite genotyping from tissue samples provides an additional indication for the absence of species bias in genotyping amplifications. Three Eimeria species were found infecting house mice at different prevalences: Eimeria ferrisi (16.7%; 95% CI 13.2 − 20.7), E. falciformis (4.2%; 95% CI 2.6 − 6.8) and E. vermiformis (1.9%; 95% CI 0.9 − 3.8). We also find that mice in dense populations are more likely to be infected with E. falciformis and E. ferrisi. We provide methods for the assessment of prevalences of coccidia at the species level in rodent systems. We show and discuss how such data can help to test hypotheses in ecology, evolution and epidemiology on a species level.
Victor Hugo Jarquindiaz - One of the best experts on this subject based on the ideXlab platform.
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detection and quantification of house mouse eimeria at the species level challenges and solutions for the assessment of coccidia in wildlife
International journal for parasitology. Parasites and wildlife, 2019Co-Authors: Victor Hugo Jarquindiaz, Alice Balard, Jenny Jost, Julia Kraft, Mert Naci Dikmen, Jana Kvicerova, Emanuel HeitlingerAbstract:Abstract Detection and quantification of coccidia in studies of wildlife can be challenging. Therefore, prevalence of coccidia is often not assessed at the Parasite species level in non-livestock animals. Parasite species – specific prevalences are especially important when studying evolutionary questions in wild populations. We tested whether increased host population density increases prevalence of individual Eimeria species at the farm level, as predicted by epidemiological theory. We studied free-living commensal populations of the house mouse (Mus musculus) in Germany, and established a strategy to detect and quantify Eimeria infections. We show that a novel diagnostic primer targeting the apicoplast genome (Ap5) and coprological assessment after flotation provide complementary detection results increasing sensitivity. Genotyping PCRs confirm detection in a subset of samples and cross-validation of different PCR markers does not indicate bias towards a particular Parasite species in genotyping. We were able to detect double infections and to determine the preferred niche of each Parasite species along the distal-proximal axis of the Intestine. Parasite genotyping from tissue samples provides additional indication for the absence of species bias in genotyping amplifications. Three Eimeria species were found infecting house mice at different prevalences: Eimeria ferrisi (16.7%; 95% CI 13.2–20.7), E. falciformis (4.2%; 95% CI 2.6–6.8) and E. vermiformis (1.9%; 95% CI 0.9–3.8). We also find that mice in dense populations are more likely to be infected with E. falciformis and E. ferrisi. We provide methods for the assessment of prevalences of coccidia at the species level in rodent systems. We show and discuss how such data can help to test hypotheses in ecology, evolution and epidemiology on a species level.
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detection and quantification of house mouse eimeria at the species level challenges and solutions for the assessment of coccidia in wildlife
bioRxiv, 2019Co-Authors: Victor Hugo Jarquindiaz, Alice Balard, Jenny Jost, Julia Kraft, Mert Naci Dikmen, Jana Kvicerova, Emanuel HeitlingerAbstract:Detection and quantification of coccidia in studies of wildlife can be challenging. Therefore, the prevalence of coccidia is often not assessed at the Parasite species level in non-livestock animals. Parasite species-specific prevalences are especially important when studying evolutionary questions in wild populations. We tested whether increased host population density increases the prevalence of individual Eimeria species at the farm level, as predicted by epidemiological theory. We studied free-living commensal populations of the house mouse (Mus musculus) in Germany and established a strategy to detect and quantify Eimeria infections. We show that a novel diagnostic primer targeting the apicoplast genome (Ap5) and coprological assessment after flotation provide complementary detection results increasing sensitivity. Genotyping PCRs confirm detection in a subset of samples and cross-validation of different PCR markers does not indicate a bias towards a particular Parasite species in genotyping. We were able to detect double infections and to determine the preferred niche of each Parasite species along the distal-proximal axis of the Intestine. Parasite genotyping from tissue samples provides an additional indication for the absence of species bias in genotyping amplifications. Three Eimeria species were found infecting house mice at different prevalences: Eimeria ferrisi (16.7%; 95% CI 13.2 − 20.7), E. falciformis (4.2%; 95% CI 2.6 − 6.8) and E. vermiformis (1.9%; 95% CI 0.9 − 3.8). We also find that mice in dense populations are more likely to be infected with E. falciformis and E. ferrisi. We provide methods for the assessment of prevalences of coccidia at the species level in rodent systems. We show and discuss how such data can help to test hypotheses in ecology, evolution and epidemiology on a species level.
Alice Balard - One of the best experts on this subject based on the ideXlab platform.
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detection and quantification of house mouse eimeria at the species level challenges and solutions for the assessment of coccidia in wildlife
International journal for parasitology. Parasites and wildlife, 2019Co-Authors: Victor Hugo Jarquindiaz, Alice Balard, Jenny Jost, Julia Kraft, Mert Naci Dikmen, Jana Kvicerova, Emanuel HeitlingerAbstract:Abstract Detection and quantification of coccidia in studies of wildlife can be challenging. Therefore, prevalence of coccidia is often not assessed at the Parasite species level in non-livestock animals. Parasite species – specific prevalences are especially important when studying evolutionary questions in wild populations. We tested whether increased host population density increases prevalence of individual Eimeria species at the farm level, as predicted by epidemiological theory. We studied free-living commensal populations of the house mouse (Mus musculus) in Germany, and established a strategy to detect and quantify Eimeria infections. We show that a novel diagnostic primer targeting the apicoplast genome (Ap5) and coprological assessment after flotation provide complementary detection results increasing sensitivity. Genotyping PCRs confirm detection in a subset of samples and cross-validation of different PCR markers does not indicate bias towards a particular Parasite species in genotyping. We were able to detect double infections and to determine the preferred niche of each Parasite species along the distal-proximal axis of the Intestine. Parasite genotyping from tissue samples provides additional indication for the absence of species bias in genotyping amplifications. Three Eimeria species were found infecting house mice at different prevalences: Eimeria ferrisi (16.7%; 95% CI 13.2–20.7), E. falciformis (4.2%; 95% CI 2.6–6.8) and E. vermiformis (1.9%; 95% CI 0.9–3.8). We also find that mice in dense populations are more likely to be infected with E. falciformis and E. ferrisi. We provide methods for the assessment of prevalences of coccidia at the species level in rodent systems. We show and discuss how such data can help to test hypotheses in ecology, evolution and epidemiology on a species level.
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detection and quantification of house mouse eimeria at the species level challenges and solutions for the assessment of coccidia in wildlife
bioRxiv, 2019Co-Authors: Victor Hugo Jarquindiaz, Alice Balard, Jenny Jost, Julia Kraft, Mert Naci Dikmen, Jana Kvicerova, Emanuel HeitlingerAbstract:Detection and quantification of coccidia in studies of wildlife can be challenging. Therefore, the prevalence of coccidia is often not assessed at the Parasite species level in non-livestock animals. Parasite species-specific prevalences are especially important when studying evolutionary questions in wild populations. We tested whether increased host population density increases the prevalence of individual Eimeria species at the farm level, as predicted by epidemiological theory. We studied free-living commensal populations of the house mouse (Mus musculus) in Germany and established a strategy to detect and quantify Eimeria infections. We show that a novel diagnostic primer targeting the apicoplast genome (Ap5) and coprological assessment after flotation provide complementary detection results increasing sensitivity. Genotyping PCRs confirm detection in a subset of samples and cross-validation of different PCR markers does not indicate a bias towards a particular Parasite species in genotyping. We were able to detect double infections and to determine the preferred niche of each Parasite species along the distal-proximal axis of the Intestine. Parasite genotyping from tissue samples provides an additional indication for the absence of species bias in genotyping amplifications. Three Eimeria species were found infecting house mice at different prevalences: Eimeria ferrisi (16.7%; 95% CI 13.2 − 20.7), E. falciformis (4.2%; 95% CI 2.6 − 6.8) and E. vermiformis (1.9%; 95% CI 0.9 − 3.8). We also find that mice in dense populations are more likely to be infected with E. falciformis and E. ferrisi. We provide methods for the assessment of prevalences of coccidia at the species level in rodent systems. We show and discuss how such data can help to test hypotheses in ecology, evolution and epidemiology on a species level.
Jana Kvicerova - One of the best experts on this subject based on the ideXlab platform.
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detection and quantification of house mouse eimeria at the species level challenges and solutions for the assessment of coccidia in wildlife
International journal for parasitology. Parasites and wildlife, 2019Co-Authors: Victor Hugo Jarquindiaz, Alice Balard, Jenny Jost, Julia Kraft, Mert Naci Dikmen, Jana Kvicerova, Emanuel HeitlingerAbstract:Abstract Detection and quantification of coccidia in studies of wildlife can be challenging. Therefore, prevalence of coccidia is often not assessed at the Parasite species level in non-livestock animals. Parasite species – specific prevalences are especially important when studying evolutionary questions in wild populations. We tested whether increased host population density increases prevalence of individual Eimeria species at the farm level, as predicted by epidemiological theory. We studied free-living commensal populations of the house mouse (Mus musculus) in Germany, and established a strategy to detect and quantify Eimeria infections. We show that a novel diagnostic primer targeting the apicoplast genome (Ap5) and coprological assessment after flotation provide complementary detection results increasing sensitivity. Genotyping PCRs confirm detection in a subset of samples and cross-validation of different PCR markers does not indicate bias towards a particular Parasite species in genotyping. We were able to detect double infections and to determine the preferred niche of each Parasite species along the distal-proximal axis of the Intestine. Parasite genotyping from tissue samples provides additional indication for the absence of species bias in genotyping amplifications. Three Eimeria species were found infecting house mice at different prevalences: Eimeria ferrisi (16.7%; 95% CI 13.2–20.7), E. falciformis (4.2%; 95% CI 2.6–6.8) and E. vermiformis (1.9%; 95% CI 0.9–3.8). We also find that mice in dense populations are more likely to be infected with E. falciformis and E. ferrisi. We provide methods for the assessment of prevalences of coccidia at the species level in rodent systems. We show and discuss how such data can help to test hypotheses in ecology, evolution and epidemiology on a species level.
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detection and quantification of house mouse eimeria at the species level challenges and solutions for the assessment of coccidia in wildlife
bioRxiv, 2019Co-Authors: Victor Hugo Jarquindiaz, Alice Balard, Jenny Jost, Julia Kraft, Mert Naci Dikmen, Jana Kvicerova, Emanuel HeitlingerAbstract:Detection and quantification of coccidia in studies of wildlife can be challenging. Therefore, the prevalence of coccidia is often not assessed at the Parasite species level in non-livestock animals. Parasite species-specific prevalences are especially important when studying evolutionary questions in wild populations. We tested whether increased host population density increases the prevalence of individual Eimeria species at the farm level, as predicted by epidemiological theory. We studied free-living commensal populations of the house mouse (Mus musculus) in Germany and established a strategy to detect and quantify Eimeria infections. We show that a novel diagnostic primer targeting the apicoplast genome (Ap5) and coprological assessment after flotation provide complementary detection results increasing sensitivity. Genotyping PCRs confirm detection in a subset of samples and cross-validation of different PCR markers does not indicate a bias towards a particular Parasite species in genotyping. We were able to detect double infections and to determine the preferred niche of each Parasite species along the distal-proximal axis of the Intestine. Parasite genotyping from tissue samples provides an additional indication for the absence of species bias in genotyping amplifications. Three Eimeria species were found infecting house mice at different prevalences: Eimeria ferrisi (16.7%; 95% CI 13.2 − 20.7), E. falciformis (4.2%; 95% CI 2.6 − 6.8) and E. vermiformis (1.9%; 95% CI 0.9 − 3.8). We also find that mice in dense populations are more likely to be infected with E. falciformis and E. ferrisi. We provide methods for the assessment of prevalences of coccidia at the species level in rodent systems. We show and discuss how such data can help to test hypotheses in ecology, evolution and epidemiology on a species level.
Mert Naci Dikmen - One of the best experts on this subject based on the ideXlab platform.
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detection and quantification of house mouse eimeria at the species level challenges and solutions for the assessment of coccidia in wildlife
International journal for parasitology. Parasites and wildlife, 2019Co-Authors: Victor Hugo Jarquindiaz, Alice Balard, Jenny Jost, Julia Kraft, Mert Naci Dikmen, Jana Kvicerova, Emanuel HeitlingerAbstract:Abstract Detection and quantification of coccidia in studies of wildlife can be challenging. Therefore, prevalence of coccidia is often not assessed at the Parasite species level in non-livestock animals. Parasite species – specific prevalences are especially important when studying evolutionary questions in wild populations. We tested whether increased host population density increases prevalence of individual Eimeria species at the farm level, as predicted by epidemiological theory. We studied free-living commensal populations of the house mouse (Mus musculus) in Germany, and established a strategy to detect and quantify Eimeria infections. We show that a novel diagnostic primer targeting the apicoplast genome (Ap5) and coprological assessment after flotation provide complementary detection results increasing sensitivity. Genotyping PCRs confirm detection in a subset of samples and cross-validation of different PCR markers does not indicate bias towards a particular Parasite species in genotyping. We were able to detect double infections and to determine the preferred niche of each Parasite species along the distal-proximal axis of the Intestine. Parasite genotyping from tissue samples provides additional indication for the absence of species bias in genotyping amplifications. Three Eimeria species were found infecting house mice at different prevalences: Eimeria ferrisi (16.7%; 95% CI 13.2–20.7), E. falciformis (4.2%; 95% CI 2.6–6.8) and E. vermiformis (1.9%; 95% CI 0.9–3.8). We also find that mice in dense populations are more likely to be infected with E. falciformis and E. ferrisi. We provide methods for the assessment of prevalences of coccidia at the species level in rodent systems. We show and discuss how such data can help to test hypotheses in ecology, evolution and epidemiology on a species level.
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detection and quantification of house mouse eimeria at the species level challenges and solutions for the assessment of coccidia in wildlife
bioRxiv, 2019Co-Authors: Victor Hugo Jarquindiaz, Alice Balard, Jenny Jost, Julia Kraft, Mert Naci Dikmen, Jana Kvicerova, Emanuel HeitlingerAbstract:Detection and quantification of coccidia in studies of wildlife can be challenging. Therefore, the prevalence of coccidia is often not assessed at the Parasite species level in non-livestock animals. Parasite species-specific prevalences are especially important when studying evolutionary questions in wild populations. We tested whether increased host population density increases the prevalence of individual Eimeria species at the farm level, as predicted by epidemiological theory. We studied free-living commensal populations of the house mouse (Mus musculus) in Germany and established a strategy to detect and quantify Eimeria infections. We show that a novel diagnostic primer targeting the apicoplast genome (Ap5) and coprological assessment after flotation provide complementary detection results increasing sensitivity. Genotyping PCRs confirm detection in a subset of samples and cross-validation of different PCR markers does not indicate a bias towards a particular Parasite species in genotyping. We were able to detect double infections and to determine the preferred niche of each Parasite species along the distal-proximal axis of the Intestine. Parasite genotyping from tissue samples provides an additional indication for the absence of species bias in genotyping amplifications. Three Eimeria species were found infecting house mice at different prevalences: Eimeria ferrisi (16.7%; 95% CI 13.2 − 20.7), E. falciformis (4.2%; 95% CI 2.6 − 6.8) and E. vermiformis (1.9%; 95% CI 0.9 − 3.8). We also find that mice in dense populations are more likely to be infected with E. falciformis and E. ferrisi. We provide methods for the assessment of prevalences of coccidia at the species level in rodent systems. We show and discuss how such data can help to test hypotheses in ecology, evolution and epidemiology on a species level.
