The Experts below are selected from a list of 705 Experts worldwide ranked by ideXlab platform
Thomas M Fandel - One of the best experts on this subject based on the ideXlab platform.
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both immediate and delayed Intracavernous Injection of autologous adipose derived stromal vascular fraction enhances recovery of erectile function in a rat model of cavernous nerve injury
European Urology, 2012Co-Authors: Thomas M Fandel, Ludovic Ferretti, Maarten Albersen, Haiyang Zhang, Tania SchroederAbstract:Abstract Background Intracavernous Injection of cultured adipose-derived stem cells (ADSCs) effectively restores erectile function in cavernous nerve (CN)–injured rats when administered at the time of injury. However, culturing exposes ADSCs to the risk of contamination and dedifferentiation. Objective Explore the effect of uncultured autologous adipose-derived stromal vascular fraction (SVF) on improving erectile function in a rat model of CN injury when administered at the time of injury or 4 wk after injury. Design, setting, and participants Eighty-nine male Sprague Dawley rats were randomly divided into four groups. CN injury or sham surgery was performed at the start of the study, and rats were treated with either SVF or vehicle. Functional testing and histologic analysis were performed 12 wk after CN crush or sham surgery. Intervention We used Intracavernous Injection of saline immediately after CN crush ( n =23), Intracavernous Injection of SVF immediately after CN crush ( n =17), Intracavernous Injection of SVF 4 wk after CN crush ( n =23), or sham surgery ( n =26). Outcome measurements and statistical analysis We studied Intracavernous pressure (ICP) response to CN electrostimulation and performed histologic examination of midpenile cross-sections. Data were analyzed using one-way analysis of variance followed by the Tukey-Kramer test. Results and limitations Both immediate and delayed treatment with SVF resulted in a significantly increased ICP-to-mean arterial pressure ratio compared with the vehicle-treated group. Both immediate and delayed treatment with SVF significantly increased expression of neuronal nitric oxide synthase and neurofilament in dorsal penile nerves compared to the vehicle group. Furthermore, the smooth muscle-to-collagen ratio within the corpus cavernosum was significantly improved in both of the SVF groups compared to vehicle-treated rats. The main limitation of the study is the lack of determination of the SVF components. Conclusions Uncultured autologous SVF injected immediately or 4 wk after CN crush improved erectile function, promoted nerve regeneration, and prevented fibrosis of the corpus cavernosum following CN injury.
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the effect of Intracavernous Injection of adipose tissue derived stem cells on hyperlipidemia associated erectile dysfunction in a rat model
The Journal of Sexual Medicine, 2010Co-Authors: Thomas M Fandel, Alan W Shindel, Yun Ching Huang, Hongxiu Ning, Ahmed M HarrazAbstract:Introduction Hyperlipidemia has been associated with erectile dysfunction (ED) via damage to the cavernous endothelium and nerves. Adipose tissue-derived stem cells (ADSC) have been shown to differentiate into endothelial cells and secrete vasculotrophic and neurotrophic factors.
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Intracavernous growth differentiation factor 5 therapy enhances the recovery of erectile function in a rat model of cavernous nerve injury
The Journal of Sexual Medicine, 2008Co-Authors: Thomas M Fandel, Anthony J Bella, Guiting Lin, Kavirach Tantiwongse, Chingshwun Lin, Jens Pohl, Tom F LueAbstract:ABSTRACT Introduction Neurogenic erectile dysfunction remains a serious complication in the postprostatectomy population. Effective protective and regenerative neuromodulatory strategies are needed. Aim To determine the effect of growth differentiation factor‐5 (GDF‐5) on erectile function and its mechanism in a rat model of cavernous nerve (CN) injury. Main Outcome Measures Erectile function was assessed by CN electrostimulation at 4 weeks. Penile tissues were examined by real‐time polymerase chain reaction (PCR) and immunohistochemical analyses. Methods Forty‐eight male Sprague‐Dawley rats were randomly divided into six equal groups: one group underwent sham operation (uninjured controls), while five groups underwent bilateral CN crush. Crush‐injury groups were treated at the time of injury with Intracavernous Injection of a slow‐release suspension of liquid microparticles containing no GDF‐5 (vehicle), 0.4 µg (low concentration), 2 µg (intermediate concentration), or 10 µg GDF‐5 (high concentration). One untreated group served as injured controls. Results GDF‐5 enhanced erectile recovery and significantly increased Intracavernous pressure in the low and intermediate‐concentration groups vs. injured controls. Low‐concentration GDF‐5 demonstrated the best functional preservation, as the Intracavernous pressure increase in this group did not differ significantly from uninjured controls. A dose‐response relationship was confirmed for the effects of GDF‐5 in penile tissue. Low‐concentration GDF‐5 showed better preservation of the penile dorsal nerves and antiapoptotic effects in the corpus cavernosum ( P Conclusion Intracavernous Injection of low (0.4 µg) or intermediate‐concentration GDF‐5 (2 µg) was effective in preserving erectile function in a rat model of neurogenic erectile dysfunction. The underlying mechanism appears to involve neuron preservation and antiapoptosis. Fandel TM, Bella AJ, Lin G, Tantiwongse K, Lin C‐S, Pohl J, and Lue TF. Intracavernous growth differentiation factor‐5 therapy enhances the recovery of erectile function in a rat model of cavernous nerve injury. J Sex Med 2008;5:1866–1875.
Run Wang - One of the best experts on this subject based on the ideXlab platform.
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nanoparticle improved stem cell therapy for erectile dysfunction in a rat model of cavernous nerve injury
The Journal of Urology, 2016Co-Authors: Haocheng Lin, Nadeem N Dhanani, Hubert Tseng, Glauco R Souza, Grace Wang, Yanna Cao, Hui Jiang, Run WangAbstract:Purpose: Recently Intracavernous Injection of stem cells has garnered great interest as a potential treatment of erectile dysfunction. However, most stem cells are washed out immediately after Intracavernous Injection. The goal of this study was to investigate using NanoShuttle™ magnetic nanoparticles to maintain stem cells in the corpus cavernosum after Intracavernous Injection, thereby improving stem cell therapy of erectile dysfunction in an animal model.Materials and Methods: Adipose derived stem cells were magnetized with NanoShuttle magnetic nanoparticles to create Nano-adipose derived stem cells. A total of 24 rats underwent bilateral cavernous nerve crush and were randomly assigned to 3 groups, including adipose derived stem cells, Nano-adipose derived stem cells and Nano-adipose derived stem cells plus magnet. Cells were tracked at days 1, 3, 5 and 9 after Intracavernous Injection. Another 40 rats with bilateral cavernous nerve crush were randomly assigned to 4 groups, including bilateral caverno...
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combined strategy of mesenchymal stem cell Injection with vascular endothelial growth factor gene therapy for the treatment of diabetes associated erectile dysfunction
Journal of Andrology, 2012Co-Authors: Xuefeng Qiu, Haocheng Lin, Run Wang, Chao Sun, Zhaorui Sun, Yun Chen, Yutian DaiAbstract:ABSTRACT: This study was designed to investigate the effect of vascular endothelial growth factor 164 adenovirus (Ad-VEGF164)–transfected mesenchymal stem cells (MSC) on improving erectile function in diabetic rats. Forty-five male Sprague-Dawley rats were injected with streptozotocin to develop type 1 diabetes, whereas 10 served as normal controls. Diabetic rats were randomly divided into 3 groups: rats that underwent Intracavernous Injection with phosphate-buffered saline (DM+PBS), unmodified MSCs (DM+MSC), and Ad-VEGF164–transfected MSCs (DM+VMSC). Normal controls received Intracavernous Injection of PBS. Four weeks after Injection, erectile function was measured by cavernous nerve electrostimulation. Penile tissue was harvested for histology and enzyme-linked immunoassay. Prior to Injection, high expression of VEGF was confirmed in Ad-VEGF164–transfected MSCs by enzyme-linked immunoassay. Four weeks after Injection, the erectile function, as well as the content of smooth muscle and endothelium in corpus cavernosum increased significantly in the MSC-injected groups compared with the DM+PBS group. There was a significant improvement of erectile function, the content of smooth muscle and endothelium, and the VEGF concentration in the corpus cavernosum in the DM+VMSC group compared with the DM+MSC group. Our study validates the effect of Intracavernous Injection of MSCs for diabetes-associated erectile dysfunction in an animal model. The combined strategy of MSC Injection with VEGF gene therapy–enhanced therapy of MSCs for the treatment of diabetes-associated erectile dysfunction.
Yun Ching Huang - One of the best experts on this subject based on the ideXlab platform.
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the effect of Intracavernous Injection of adipose tissue derived stem cells on hyperlipidemia associated erectile dysfunction in a rat model
The Journal of Sexual Medicine, 2010Co-Authors: Thomas M Fandel, Alan W Shindel, Yun Ching Huang, Hongxiu Ning, Ahmed M HarrazAbstract:Introduction Hyperlipidemia has been associated with erectile dysfunction (ED) via damage to the cavernous endothelium and nerves. Adipose tissue-derived stem cells (ADSC) have been shown to differentiate into endothelial cells and secrete vasculotrophic and neurotrophic factors.
Haocheng Lin - One of the best experts on this subject based on the ideXlab platform.
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nanoparticle improved stem cell therapy for erectile dysfunction in a rat model of cavernous nerve injury
The Journal of Urology, 2016Co-Authors: Haocheng Lin, Nadeem N Dhanani, Hubert Tseng, Glauco R Souza, Grace Wang, Yanna Cao, Hui Jiang, Run WangAbstract:Purpose: Recently Intracavernous Injection of stem cells has garnered great interest as a potential treatment of erectile dysfunction. However, most stem cells are washed out immediately after Intracavernous Injection. The goal of this study was to investigate using NanoShuttle™ magnetic nanoparticles to maintain stem cells in the corpus cavernosum after Intracavernous Injection, thereby improving stem cell therapy of erectile dysfunction in an animal model.Materials and Methods: Adipose derived stem cells were magnetized with NanoShuttle magnetic nanoparticles to create Nano-adipose derived stem cells. A total of 24 rats underwent bilateral cavernous nerve crush and were randomly assigned to 3 groups, including adipose derived stem cells, Nano-adipose derived stem cells and Nano-adipose derived stem cells plus magnet. Cells were tracked at days 1, 3, 5 and 9 after Intracavernous Injection. Another 40 rats with bilateral cavernous nerve crush were randomly assigned to 4 groups, including bilateral caverno...
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combined strategy of mesenchymal stem cell Injection with vascular endothelial growth factor gene therapy for the treatment of diabetes associated erectile dysfunction
Journal of Andrology, 2012Co-Authors: Xuefeng Qiu, Haocheng Lin, Run Wang, Chao Sun, Zhaorui Sun, Yun Chen, Yutian DaiAbstract:ABSTRACT: This study was designed to investigate the effect of vascular endothelial growth factor 164 adenovirus (Ad-VEGF164)–transfected mesenchymal stem cells (MSC) on improving erectile function in diabetic rats. Forty-five male Sprague-Dawley rats were injected with streptozotocin to develop type 1 diabetes, whereas 10 served as normal controls. Diabetic rats were randomly divided into 3 groups: rats that underwent Intracavernous Injection with phosphate-buffered saline (DM+PBS), unmodified MSCs (DM+MSC), and Ad-VEGF164–transfected MSCs (DM+VMSC). Normal controls received Intracavernous Injection of PBS. Four weeks after Injection, erectile function was measured by cavernous nerve electrostimulation. Penile tissue was harvested for histology and enzyme-linked immunoassay. Prior to Injection, high expression of VEGF was confirmed in Ad-VEGF164–transfected MSCs by enzyme-linked immunoassay. Four weeks after Injection, the erectile function, as well as the content of smooth muscle and endothelium in corpus cavernosum increased significantly in the MSC-injected groups compared with the DM+PBS group. There was a significant improvement of erectile function, the content of smooth muscle and endothelium, and the VEGF concentration in the corpus cavernosum in the DM+VMSC group compared with the DM+MSC group. Our study validates the effect of Intracavernous Injection of MSCs for diabetes-associated erectile dysfunction in an animal model. The combined strategy of MSC Injection with VEGF gene therapy–enhanced therapy of MSCs for the treatment of diabetes-associated erectile dysfunction.
Ahmed M Harraz - One of the best experts on this subject based on the ideXlab platform.
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the effect of Intracavernous Injection of adipose tissue derived stem cells on hyperlipidemia associated erectile dysfunction in a rat model
The Journal of Sexual Medicine, 2010Co-Authors: Thomas M Fandel, Alan W Shindel, Yun Ching Huang, Hongxiu Ning, Ahmed M HarrazAbstract:Introduction Hyperlipidemia has been associated with erectile dysfunction (ED) via damage to the cavernous endothelium and nerves. Adipose tissue-derived stem cells (ADSC) have been shown to differentiate into endothelial cells and secrete vasculotrophic and neurotrophic factors.
