The Experts below are selected from a list of 318 Experts worldwide ranked by ideXlab platform
Ok Chan Jeong - One of the best experts on this subject based on the ideXlab platform.
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Intracellular Calcium-EXPRESSION DISPLAY OPERATED BY COMPRESSIVE STRESS
2020Co-Authors: Jong Hun Jeon, Ok Chan JeongAbstract:Microcell chips with simple channels were fabricated and the compressive stress-induced Intracellular Calcium expression of those cells was observed in an investigation of the optimum duration of compressive stress. Experimental results showed that the Intracellular Calcium expression of such cells had multiple peaks, similar to mechanical oscillation, with a period of about 200 s. A seven-segment-type of Intracellular Calcium-expression-display was fabricated to demonstrate the “twinkling phenomenon” in Intracellular Calcium expression.
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Intracellular Calcium-expression-display (ICED) device operated by compressive stimulation of cells
Microelectronic Engineering, 2012Co-Authors: Ok Chan JeongAbstract:The effects of steady compressive stimulation on Intracellular Calcium expression in MG-63 human osteoblast-like bone cells were examined using a fabricated micro cell chip with a microchannel array. A computer-controlled pneumatic system was used to create compressive stress as a source of mechanical stimulation without fluid flow in the microchannel. Intracellular Calcium levels were observed using laser-scanning fluorescence microscopy and showed multiple peaks of Intracellular Calcium expression over time. To visualize the mechanosensory properties of live cells suggested by the observed periodic Intracellular Calcium expression, we designed and fabricated a seven-segment-type of Intracellular Calcium-expression-display (ICED) device. The device consisted of a glass cell-culture substrate, two polydimethylsiloxane layers for cell introduction and pneumatic stimulation, and seven individually controllable pneumatic actuators. Compressive stimulation was applied to the cells via deformation of the pneumatic actuator diaphragm. The application of external pressure to the ICED device resulted in a green fluorescence representation of Intracellular Calcium expression in the form of an ''8''.
Jeongok Chan - One of the best experts on this subject based on the ideXlab platform.
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Intracellular Calcium-expression-display (ICED) device operated by compressive stimulation of cells
Microelectronic Engineering, 2012Co-Authors: Kimtae Kyung, Jeongok ChanAbstract:Graphical abstract.Display Omitted Highlights We fabricate an Intracellular Calcium-expression-display (ICED) device. We examine effects of compressive stimulation on Intracellular Calcium expressi...
R. Benitez - One of the best experts on this subject based on the ideXlab platform.
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Identification of Intracellular Calcium dynamics in stimulated cardiomyocytes
2010 Annual International Conference of the IEEE Engineering in Medicine and Biology, 2010Co-Authors: A. Vallmitjana, M. Barriga, Z. Nenadic, A. Llach, E. Alvarez-lacalle, L. Hove-madsen, R. BenitezAbstract:We have developed an automatic method for the analysis and identification of dynamical regimes in Intracellular Calcium patterns from confocal Calcium images. The method allows the identification of different dynamical patterns such as spatially concordant and discordant alternans, irregular behavior or phase-locking regimes such as period doubling or halving. The method can be applied to the analysis of different cardiac pathologies related to anomalies at the cellular level such as ventricular reentrant arrhythmias.
W Klaus - One of the best experts on this subject based on the ideXlab platform.
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the effect of the Calcium antagonist nitrendipine on Intracellular Calcium concentration in endothelial cells
British Journal of Pharmacology, 1996Co-Authors: Aida Salameh, Gunhild Schomecker, Katja Breitkopf, S Dhein, W KlausAbstract:Abstract 1. Nitrendipine induces NO-release from coronary vascular endothelium presumably by activating endothelial NO-synthase. We have investigated whether this effect may be mediated by an influence on the Intracellular Calcium in endothelial cells. 2. Bovine aortic endothelial cells (BAEC) were incubated with Fura-2/AM (1 microM) for 30 min and Fura-2 fluorescence was measured at 510 nm in response to chopped excitation with both 340 and 380 nm. The ratio 340/380 nm (known to reflect changes in Intracellular Calcium) was calculated from these data. 3. Nitrendipine (0.1 to 100 microM) led to a significant, concentration-dependent, monophasic increase in [Ca2+]i in suspended BAEC by 11 +/- 2 nM (0.1 microM), 23 +/- 3 nM (1 microM), 34 +/- 4 nM (10 microM) and by 47 +/- 5 nM (100 microM) from a control levels of 118 +/- 10 nM. 4. This elevation of Intracellular Calcium was prevented by pretreatment of BAECs with gadolinium (100 microM) or by incubation with Calcium free saline solution. In contrast, the application of 0.3 microM thapsigargin did not abolish the nitrendipine-induced Calcium signal. In additional experiments it was shown that the nitrendipine-induced NO-release (as measured with the oxy-haemoglobin-method could also be inhibited by gadolinium and was absent in Calcium-free solution. 5. Thus, nitrendipine elevates Intracellular Calcium in suspended BAECs in a concentration-dependent manner. This elevation is mainly due to a gadolinium-sensitive Calcium influx from the extracellular space rather than a Calcium release from Intracellular stores.
Kimtae Kyung - One of the best experts on this subject based on the ideXlab platform.
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Intracellular Calcium-expression-display (ICED) device operated by compressive stimulation of cells
Microelectronic Engineering, 2012Co-Authors: Kimtae Kyung, Jeongok ChanAbstract:Graphical abstract.Display Omitted Highlights We fabricate an Intracellular Calcium-expression-display (ICED) device. We examine effects of compressive stimulation on Intracellular Calcium expressi...
