The Experts below are selected from a list of 10953 Experts worldwide ranked by ideXlab platform
Bianxiao Cui - One of the best experts on this subject based on the ideXlab platform.
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iridium oxide nanotube electrodes for sensitive and prolonged Intracellular measurement of action potentials
Nature Communications, 2014Co-Authors: Ziliang Carter Lin, Chong Xie, Yi Cui, Yasuko Osakada, Bianxiao CuiAbstract:Intracellular Recording of action potentials is important to understand electrically-excitable cells. Recently, vertical nanoelectrodes have been developed to achieve highly sensitive, minimally invasive and large-scale Intracellular Recording. It has been demonstrated that the vertical geometry is crucial for the enhanced signal detection. Here we develop nanoelectrodes of a new geometry, namely nanotubes of iridium oxide. When cardiomyocytes are cultured upon those nanotubes, the cell membrane not only wraps around the vertical tubes but also protrudes deep into the hollow centre. We show that this nanotube geometry enhances cell-electrode coupling and results in larger signals than solid nanoelectrodes. The nanotube electrodes also afford much longer Intracellular access and are minimally invasive, making it possible to achieve stable Recording up to an hour in a single session and more than 8 days of consecutive daily Recording. This study suggests that the nanoelectrode performance can be significantly improved by optimizing the electrode geometry.
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Intracellular Recording of action potentials by nanopillar electroporation
Nature Nanotechnology, 2012Co-Authors: Chong Xie, Ziliang Lin, Lindsey Hanson, Yi Cui, Bianxiao CuiAbstract:Arrays of vertical nanopillar electrodes can be used for both Intracellular and extracellular Recording with excellent signal strength and quality, and minimal damage to the cells.
Yasuko Osakada - One of the best experts on this subject based on the ideXlab platform.
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iridium oxide nanotube electrodes for sensitive and prolonged Intracellular measurement of action potentials
Nature Communications, 2014Co-Authors: Yasuko OsakadaAbstract:Nanoelectrode Intracellular Recording of action potential may be used to study cell electrophysiology. Here, the authors demonstrate vertical nanotube electrodes which improve Recording quality and duration, as the cell membrane wraps the nanotubes surfaces and protrudes into their hollow centres.
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iridium oxide nanotube electrodes for sensitive and prolonged Intracellular measurement of action potentials
Nature Communications, 2014Co-Authors: Ziliang Carter Lin, Chong Xie, Yi Cui, Yasuko Osakada, Bianxiao CuiAbstract:Intracellular Recording of action potentials is important to understand electrically-excitable cells. Recently, vertical nanoelectrodes have been developed to achieve highly sensitive, minimally invasive and large-scale Intracellular Recording. It has been demonstrated that the vertical geometry is crucial for the enhanced signal detection. Here we develop nanoelectrodes of a new geometry, namely nanotubes of iridium oxide. When cardiomyocytes are cultured upon those nanotubes, the cell membrane not only wraps around the vertical tubes but also protrudes deep into the hollow centre. We show that this nanotube geometry enhances cell-electrode coupling and results in larger signals than solid nanoelectrodes. The nanotube electrodes also afford much longer Intracellular access and are minimally invasive, making it possible to achieve stable Recording up to an hour in a single session and more than 8 days of consecutive daily Recording. This study suggests that the nanoelectrode performance can be significantly improved by optimizing the electrode geometry.
Urs Frey - One of the best experts on this subject based on the ideXlab platform.
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revealing neuronal function through microelectrode array Recordings
Frontiers in Neuroscience, 2015Co-Authors: Marie Engelene J Obien, Urs Frey, Kosmas Deligkaris, Torsten Bullmann, Douglas J BakkumAbstract:Microelectrode arrays and microprobes have been widely utilized to measure neuronal activity, both in vitro and in vivo. The key advantage is the capability to record and stimulate neurons at multiple sites simultaneously. However, unlike the single-cell or single-channel resolution of Intracellular Recording, microelectrodes detect signals from all possible sources around the sensor. Here, we review the current understanding of microelectrode signals and the techniques for analyzing them. We introduce the ongoing advancements in microelectrode technology, with focus on achieving higher resolution and quality of Recordings by means of monolithic integration with on-chip circuitry. We show how recent advanced microelectrode array measurement methods facilitate the understanding of single neurons as well as network function.
Charles M. Lieber - One of the best experts on this subject based on the ideXlab platform.
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Scalable ultrasmall three-dimensional nanowire transistor probes for Intracellular Recording
NATURE PUBLISHING GROUP, 2019Co-Authors: Yunlong Zhao, Anqi Zhang, Siheng Sean You, Jae-hyun Lee, Jinlin Huang, Charles M. LieberAbstract:© 2019, The Author(s), under exclusive licence to Springer Nature Limited.New tools for Intracellular electrophysiology that push the limits of spatiotemporal resolution while reducing invasiveness could provide a deeper understanding of electrogenic cells and their networks in tissues, and push progress towards human–machine interfaces. Although significant advances have been made in developing nanodevices for Intracellular probes, current approaches exhibit a trade-off between device scalability and Recording amplitude. We address this challenge by combining deterministic shape-controlled nanowire transfer with spatially defined semiconductor-to-metal transformation to realize scalable nanowire field-effect transistor probe arrays with controllable tip geometry and sensor size, which enable Recording of up to 100 mV Intracellular action potentials from primary neurons. Systematic studies on neurons and cardiomyocytes show that controlling device curvature and sensor size is critical for achieving high-amplitude Intracellular Recordings. In addition, this device design allows for multiplexed Recording from single cells and cell networks and could enable future investigations of dynamics in the brain and other tissue
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Outside looking in: nanotube transistor Intracellular sensors.
2018Co-Authors: Ruixuan Gao, Quan Qing, Steffen Strehle, Bozhi Tian, Itzhaq Cohen-karni, Ping Xie, Xiaojie Duan, Charles M. LieberAbstract:Nanowire-based field-effect transistors, including devices with planar and three-dimensional configurations, are being actively explored as detectors for extra- and Intracellular Recording due to their small size and high sensitivities. Here we report the synthesis, fabrication, and characterization of a new needle-shaped nanoprobe based on an active silicon nanotube transistor, ANTT, that enables high-resolution Intracellular Recording. In the ANTT probe, the source/drain contacts to the silicon nanotube are fabricated on one end, passivated from external solution, and then time-dependent changes in potential can be recorded from the opposite nanotube end via the solution filling the tube. Measurements of conductance versus water-gate potential in aqueous solution show that the ANTT probe is selectively gated by potential changes within the nanotube, thus demonstrating the basic operating principle of the ANTT device. Studies interfacing the ANTT probe with spontaneously beating cardiomyocytes yielded stable Intracellular action potentials similar to those reported by other electrophysiological techniques. In addition, the straightforward fabrication of ANTT devices was exploited to prepare multiple ANTT structures at the end of single probes, which enabled multiplexed Recording of Intracellular action potentials from single cells and multiplexed arrays of single ANTT device probes. These studies open up unique opportunities for multisite Recordings from individual cells through cellular networks.
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Beyond the patch clamp: nanotechnologies for Intracellular Recording.
Neuron, 2015Co-Authors: Peter B. Kruskal, Teng Gao, Zhe Jiang, Charles M. LieberAbstract:The patch clamp is a fundamental tool for neuroscientists, offering insights that have shaped our understanding of the brain. Advances in nanotechnology suggest that the next generation of Recording methods is now within reach. We discuss the complexity and future promise of applying nanoscience to neural Recording.
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kinked p n junction nanowire probes for high spatial resolution sensing and Intracellular Recording
Nano Letters, 2012Co-Authors: Zhe Jiang, Quan Qing, Charles M. LieberAbstract:Semiconductor nanowires and other semiconducting nanoscale materials configured as field-effect transistors have been studied extensively as biological/chemical (bio/chem) sensors. These nanomaterials have demonstrated high-sensitivity from one- and two-dimensional sensors, although the realization of the ultimate pointlike detector has not been achieved. In this regard, nanoscale p–n diodes are attractive since the device element is naturally localized near the junction, and while nanowire p–n diodes have been widely studied as photovoltaic devices, their applications as bio/chem sensors have not been explored. Here we demonstrate that p–n diode devices can serve as a new and powerful family of highly localized biosensor probes. Designed nanoscale axial p–n junctions were synthetically introduced at the joints of kinked silicon nanowires. Scanning electron microscopy images showed that the kinked nanowire structures were achieved, and electrical transport measurements exhibited rectifying behavior with w...
Pedro Jorge Caldas Magalhaes - One of the best experts on this subject based on the ideXlab platform.
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effects of 1 8 cineole on electrophysiological parameters of neurons of the rat superior cervical ganglion
Clinical and Experimental Pharmacology and Physiology, 2009Co-Authors: Francisco Walber Ferreiradasilva, Roseli Barbosa, Luiz Moreirajunior, Tiago Dos Santosnascimento, Maria D De Oliveiramartins, Andrelina Noronha Coelhodesouza, Francisco Sales Avila Cavalcante, Vânia Marilande Ceccatto, Telma L G Lemos, Pedro Jorge Caldas MagalhaesAbstract:SUMMARY 1. 1,8-Cineole is a non-toxic small terpenoid oxide believed to have medicinal properties in folk medicine. It has been shown to have various pharmacological effects, including blockade of the compound action potential (AP). In the present study, using Intracellular Recording techniques, we investigated the effects of 1,8-cineole on the electrophysiological parameters of neurons of the superior cervical ganglion (SCG) in rats.
