The Experts below are selected from a list of 11094 Experts worldwide ranked by ideXlab platform
Giovanni Ponti - One of the best experts on this subject based on the ideXlab platform.
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multiphoton laser tomography and fluorescence lifetime imaging of melanoma morphologic features and quantitative data for sensitive and specific non Invasive Diagnostics
PLOS ONE, 2013Co-Authors: Stefania Seidenari, Federica Arginelli, Christopher Dunsby, P M W French, Karsten Konig, Cristina Magnoni, Clifford Talbot, Giovanni PontiAbstract:Multiphoton laser tomography (MPT) combined with fluorescence lifetime imaging (FLIM) is a non-Invasive imaging technique, based on the study of fluorescence decay times of naturally occurring fluorescent molecules, enabling a non-Invasive investigation of the skin with subcellular resolution. The aim of this retrospective observational ex vivo study, was to characterize melanoma both from a morphologic and a quantitative point of view, attaining an improvement in the diagnostic accuracy with respect to dermoscopy. In the training phase, thirty parameters, comprising both cytological descriptors and architectural aspects, were identified. The training set included 6 melanomas with a mean Breslow thickness±S.D. of 0.89±0.48 mm. In the test phase, these parameters were blindly evaluated on a test data set consisting of 25 melanomas, 50 nevi and 50 basal cell carcinomas. Melanomas in the test phase comprised 8 in situ lesions and had a mean thickness±S.D. of 0.77±1.2 mm. Moreover, quantitative FLIM data were calculated for special areas of interest. Melanoma was characterized by the presence of atypical short lifetime cells and architectural disorder, in contrast to nevi presenting typical cells and a regular histoarchitecture. Sensitivity and specificity values for melanoma diagnosis were 100% and 98%, respectively, whereas dermoscopy achieved the same sensitivity, but a lower specificity (82%). Mean fluorescence lifetime values of melanocytic cells did not vary between melanomas and nevi, but significantly differed from those referring to basal cell carcinoma enabling a differential diagnosis based on quantitative data. Data from prospective preoperative trials are needed to confirm if MPT/FLIM could increase diagnostic specificity and thus reduce unnecessary surgical excisions.
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multiphoton laser tomography and fluorescence lifetime imaging of basal cell carcinoma morphologic features for non Invasive Diagnostics
Experimental Dermatology, 2012Co-Authors: Stefania Seidenari, Federica Arginelli, Christopher Dunsby, P M W French, Karsten Konig, Cristina Magnoni, Marco Manfredini, Clifford Talbot, Giovanni PontiAbstract:: Multiphoton laser tomography (MPT) combined with fluorescence lifetime imaging (FLIM) is a non-Invasive imaging technique, which gives access to the cellular and extracellular morphology of the skin. The aim of our study was to assess the sensitivity and specificity of MPT/FLIM descriptors for basal cell carcinoma (BCC), to improve BCC diagnosis and the identification of tumor margins. In the preliminary study, FLIM images referring to 35 BCCs and 35 healthy skin samples were evaluated for the identification of morphologic descriptors characteristic of BCC. In the main study, the selected parameters were blindly evaluated on a test set comprising 63 BCCs, 63 healthy skin samples and 66 skin lesions. Moreover, FLIM values inside a region of interest were calculated on 98 healthy skin and 98 BCC samples. In the preliminary study, three epidermal descriptors and 7 BCC descriptors were identified. The specificity of the diagnostic criteria versus 'other lesions' was extremely high, indicating that the presence of at least one BCC descriptor makes the diagnosis of 'other lesion' extremely unlikely. FLIM values referring to BCC cells significantly differed from those of healthy skin. In this study, we identified morphological and numerical descriptors enabling the differentiation of BCC from other skin disorders and its distinction from healthy skin in ex vivo samples. In future, MPT/FLIM may be applied to skin lesions to provide direct clinical guidance before biopsy and histological examination and for the identification of tumor margins allowing a complete surgical removal.
Marica B Ericson - One of the best experts on this subject based on the ideXlab platform.
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multiphoton laser scanning microscopy on non melanoma skin cancer morphologic features for future non Invasive Diagnostics
Journal of Investigative Dermatology, 2008Co-Authors: John Paoli, Maria Smedh, Annmarie Wennberg, Marica B EricsonAbstract:This study describes the morphologic features of human non-melanoma skin cancer obtained using multiphoton laser scanning microscopy (MPLSM) on freshly excised specimens from 14 patients. Optical sectioning parallel to the tissue surface was performed, resulting in en face autofluorescence images of the epidermis and upper dermis, reaching tissue depths of 135mm. The microscopy was carried out ex vivo using a femtosecond pulsed laser at 780nm and a 40/0.8 objective. The autofluorescence was detected in the range of 450–530nm. Traditional histopathological criteria such as bowenoid dysplasia, multinucleated cells, or hyperkeratosis in squamous cell carcinoma in situ (SCCIS) (five specimens), and peripheral palisading of tumor cells in superficial basal cell carcinoma (SBCC) (six specimens) were clearly discerned. The morphologic features differed significantly between these lesions and perilesional skin. However, characteristic tumor aggregates were found in only one of the three investigated nodular basal cell carcinomas (NBCCs) due to limited imaging depth. In addition, speckled perinuclear fluorescence was observed in both lesions and normal perilesional skin. In conclusion, MPLSM could potentially be applied for non-Invasive Diagnostics of SCCIS and SBCC, whereas the ability to characterize NBCC is unclear at this point.
Stefania Seidenari - One of the best experts on this subject based on the ideXlab platform.
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multiphoton laser tomography and fluorescence lifetime imaging of melanoma morphologic features and quantitative data for sensitive and specific non Invasive Diagnostics
PLOS ONE, 2013Co-Authors: Stefania Seidenari, Federica Arginelli, Christopher Dunsby, P M W French, Karsten Konig, Cristina Magnoni, Clifford Talbot, Giovanni PontiAbstract:Multiphoton laser tomography (MPT) combined with fluorescence lifetime imaging (FLIM) is a non-Invasive imaging technique, based on the study of fluorescence decay times of naturally occurring fluorescent molecules, enabling a non-Invasive investigation of the skin with subcellular resolution. The aim of this retrospective observational ex vivo study, was to characterize melanoma both from a morphologic and a quantitative point of view, attaining an improvement in the diagnostic accuracy with respect to dermoscopy. In the training phase, thirty parameters, comprising both cytological descriptors and architectural aspects, were identified. The training set included 6 melanomas with a mean Breslow thickness±S.D. of 0.89±0.48 mm. In the test phase, these parameters were blindly evaluated on a test data set consisting of 25 melanomas, 50 nevi and 50 basal cell carcinomas. Melanomas in the test phase comprised 8 in situ lesions and had a mean thickness±S.D. of 0.77±1.2 mm. Moreover, quantitative FLIM data were calculated for special areas of interest. Melanoma was characterized by the presence of atypical short lifetime cells and architectural disorder, in contrast to nevi presenting typical cells and a regular histoarchitecture. Sensitivity and specificity values for melanoma diagnosis were 100% and 98%, respectively, whereas dermoscopy achieved the same sensitivity, but a lower specificity (82%). Mean fluorescence lifetime values of melanocytic cells did not vary between melanomas and nevi, but significantly differed from those referring to basal cell carcinoma enabling a differential diagnosis based on quantitative data. Data from prospective preoperative trials are needed to confirm if MPT/FLIM could increase diagnostic specificity and thus reduce unnecessary surgical excisions.
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multiphoton laser tomography and fluorescence lifetime imaging of basal cell carcinoma morphologic features for non Invasive Diagnostics
Experimental Dermatology, 2012Co-Authors: Stefania Seidenari, Federica Arginelli, Christopher Dunsby, P M W French, Karsten Konig, Cristina Magnoni, Marco Manfredini, Clifford Talbot, Giovanni PontiAbstract:: Multiphoton laser tomography (MPT) combined with fluorescence lifetime imaging (FLIM) is a non-Invasive imaging technique, which gives access to the cellular and extracellular morphology of the skin. The aim of our study was to assess the sensitivity and specificity of MPT/FLIM descriptors for basal cell carcinoma (BCC), to improve BCC diagnosis and the identification of tumor margins. In the preliminary study, FLIM images referring to 35 BCCs and 35 healthy skin samples were evaluated for the identification of morphologic descriptors characteristic of BCC. In the main study, the selected parameters were blindly evaluated on a test set comprising 63 BCCs, 63 healthy skin samples and 66 skin lesions. Moreover, FLIM values inside a region of interest were calculated on 98 healthy skin and 98 BCC samples. In the preliminary study, three epidermal descriptors and 7 BCC descriptors were identified. The specificity of the diagnostic criteria versus 'other lesions' was extremely high, indicating that the presence of at least one BCC descriptor makes the diagnosis of 'other lesion' extremely unlikely. FLIM values referring to BCC cells significantly differed from those of healthy skin. In this study, we identified morphological and numerical descriptors enabling the differentiation of BCC from other skin disorders and its distinction from healthy skin in ex vivo samples. In future, MPT/FLIM may be applied to skin lesions to provide direct clinical guidance before biopsy and histological examination and for the identification of tumor margins allowing a complete surgical removal.
Georg Hansmann - One of the best experts on this subject based on the ideXlab platform.
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transthoracic echocardiography for the evaluation of children and adolescents with suspected or confirmed pulmonary hypertension expert consensus statement on the diagnosis and treatment of paediatric pulmonary hypertension the european paediatric pulmonary vascular disease network endorsed by ishlt and dgpk
Heart, 2016Co-Authors: Martin Koestenberger, Christian Apitz, Hashim Abdulkhaliq, Georg HansmannAbstract:Transthoracic echocardiography (TTE) is a useful method for non-Invasive screening of patients at risk of pulmonary hypertension (PH). Since TTE often serves as the initial study before Invasive cardiac catheterisation, misinterpretation of TTE variables may lead to missed or delayed diagnosis with devastating consequences for the patients, or unnecessary Invasive Diagnostics that have inheriting risks. Due to the heterogeneous anatomy in congenital heart disease, particularly the assessment of myocardial function in children with PH is challenging. Here, we present recommendations on the use of TTE in the screening, diagnosis and follow-up of patients with PH, and discuss the limitations of this non-Invasive imaging technique. This expert consensus statement focuses on key TTE variables used to determine the pressure in the pulmonary artery, myocardial contractility and systolic and diastolic function of the RV and LV. A particular focus is on the TTE assessment of RV function and geometry. According to the published data on the application of TTE in PH in childhood, we suggest a structured approach for non-Invasive assessment of pulmonary artery pressure and myocardial function that may help to identify patients with early ventricular deterioration and their response to advanced pharmacotherapy. In addition to clinical and biochemical markers, serial examination of patients with PH using a standardised TTE approach, determining conventional and several more novel echocardiographic variables may allow early diagnosis and treatment, better recognition of disease progression and guide tailored therapy.
Clifford Talbot - One of the best experts on this subject based on the ideXlab platform.
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multiphoton laser tomography and fluorescence lifetime imaging of melanoma morphologic features and quantitative data for sensitive and specific non Invasive Diagnostics
PLOS ONE, 2013Co-Authors: Stefania Seidenari, Federica Arginelli, Christopher Dunsby, P M W French, Karsten Konig, Cristina Magnoni, Clifford Talbot, Giovanni PontiAbstract:Multiphoton laser tomography (MPT) combined with fluorescence lifetime imaging (FLIM) is a non-Invasive imaging technique, based on the study of fluorescence decay times of naturally occurring fluorescent molecules, enabling a non-Invasive investigation of the skin with subcellular resolution. The aim of this retrospective observational ex vivo study, was to characterize melanoma both from a morphologic and a quantitative point of view, attaining an improvement in the diagnostic accuracy with respect to dermoscopy. In the training phase, thirty parameters, comprising both cytological descriptors and architectural aspects, were identified. The training set included 6 melanomas with a mean Breslow thickness±S.D. of 0.89±0.48 mm. In the test phase, these parameters were blindly evaluated on a test data set consisting of 25 melanomas, 50 nevi and 50 basal cell carcinomas. Melanomas in the test phase comprised 8 in situ lesions and had a mean thickness±S.D. of 0.77±1.2 mm. Moreover, quantitative FLIM data were calculated for special areas of interest. Melanoma was characterized by the presence of atypical short lifetime cells and architectural disorder, in contrast to nevi presenting typical cells and a regular histoarchitecture. Sensitivity and specificity values for melanoma diagnosis were 100% and 98%, respectively, whereas dermoscopy achieved the same sensitivity, but a lower specificity (82%). Mean fluorescence lifetime values of melanocytic cells did not vary between melanomas and nevi, but significantly differed from those referring to basal cell carcinoma enabling a differential diagnosis based on quantitative data. Data from prospective preoperative trials are needed to confirm if MPT/FLIM could increase diagnostic specificity and thus reduce unnecessary surgical excisions.
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multiphoton laser tomography and fluorescence lifetime imaging of basal cell carcinoma morphologic features for non Invasive Diagnostics
Experimental Dermatology, 2012Co-Authors: Stefania Seidenari, Federica Arginelli, Christopher Dunsby, P M W French, Karsten Konig, Cristina Magnoni, Marco Manfredini, Clifford Talbot, Giovanni PontiAbstract:: Multiphoton laser tomography (MPT) combined with fluorescence lifetime imaging (FLIM) is a non-Invasive imaging technique, which gives access to the cellular and extracellular morphology of the skin. The aim of our study was to assess the sensitivity and specificity of MPT/FLIM descriptors for basal cell carcinoma (BCC), to improve BCC diagnosis and the identification of tumor margins. In the preliminary study, FLIM images referring to 35 BCCs and 35 healthy skin samples were evaluated for the identification of morphologic descriptors characteristic of BCC. In the main study, the selected parameters were blindly evaluated on a test set comprising 63 BCCs, 63 healthy skin samples and 66 skin lesions. Moreover, FLIM values inside a region of interest were calculated on 98 healthy skin and 98 BCC samples. In the preliminary study, three epidermal descriptors and 7 BCC descriptors were identified. The specificity of the diagnostic criteria versus 'other lesions' was extremely high, indicating that the presence of at least one BCC descriptor makes the diagnosis of 'other lesion' extremely unlikely. FLIM values referring to BCC cells significantly differed from those of healthy skin. In this study, we identified morphological and numerical descriptors enabling the differentiation of BCC from other skin disorders and its distinction from healthy skin in ex vivo samples. In future, MPT/FLIM may be applied to skin lesions to provide direct clinical guidance before biopsy and histological examination and for the identification of tumor margins allowing a complete surgical removal.
