The Experts below are selected from a list of 222 Experts worldwide ranked by ideXlab platform
Jingping Yun - One of the best experts on this subject based on the ideXlab platform.
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abstract 3547 foxd3 regulated microrna 137 suppresses tumour growth and metastasis in human hepatocellular carcinoma by targeting akt2
Cancer Research, 2014Co-Authors: Lili Liu, Chris Zhiyi Zhang, Jingping YunAbstract:Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA Background and Aims: microRNAs, which are frequently deregulated in human cancer, have been implicated in the progression of hepatocarcinogenesis. Our study aims to investigate the role of miR-137 in cell proliferation and metastasis of hepatocellular carcinoma and the underlying molecular mechanisms. Methods: Real-time PCR and western blot were used to examine the expressions of miR-137, Akt2 and FoxD3 in HCC tissues and cells. The in vitro and in vivo effects of miR-137 were determined. Luciferase reporter assay was conducted to confirm the association of miR-137 and AKT2. CHIP assay was performed to test the regulation of miR-137 by FoxD3. Results: We show that microRNA (miR)-137 is significantly down-regulated in hepatocellular carcinoma (HCC), and its decreased expression is associated with vein invasion, incomplete Involucrum, and tumour metastasis. Multivariate analysis suggests that miR-137 is an independent indicator for poor survival. To determine its role in HCC development, we showed that overexpression of miR-137 suppresses cell proliferation, invasion and metastasis in vitro. Conversely, miR-137 inhibition promoted HCC cell growth. We also identified AKT2 as a key target of miR-137 in this context. Statistical data revealed a reverse correlation of AKT2 and miR-137 expression in HCC patients. Silencing of AKT2 phenotypically copied miR-137-induced phenotypes, whereas re-expression of AKT2 reversed the suppressive effects of miR-137. Further investigation showed that miR-137 exerted its anti-tumour activity via inhibiting the AKT2/mTOR pathway. Moreover, we demonstrated that FoxD3 binds to the promoter of miR-137 and activates its transcription. In vivo studies confirmed that FoxD3-regulated miR-137 inhibition of HCC growth and metastasis via directly targeting AKT2. Conclusions: Our findings indicated that miR-137 is a valuable biomarker for HCC prognosis and demonstrated that the FoxD3/miR-137/AKT2 regulatory network plays an important role in HCC progression. Citation Format: Li-Li Liu, Shi-Xun Lu, Min Li, Jia Fu, Chris Zhiyi Zhang, Jing-Ping Yun. FoxD3-regulated microRNA-137 suppresses tumour growth and metastasis in human hepatocellular carcinoma by targeting AKT2. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3547. doi:10.1158/1538-7445.AM2014-3547
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foxd3 regulated microrna 137 suppresses tumour growth and metastasis in human hepatocellular carcinoma by targeting akt2
Oncotarget, 2014Co-Authors: Lili Liu, Chris Zhiyi Zhang, Yuan Zhong Yang, Rong Zhen Luo, Jingping YunAbstract:// Li-Li Liu 1,2,* , Shi-Xun Lu 1,2,* , Min Li 1,2 , Lin-Zi Li 1,2 , Jia Fu 1,2 , Wen Hu 1,2 , Yuan-Zhong Yang 1,2 , Rong-Zhen Luo 1,2 , Chris Zhiyi Zhang 1,2 , Jing-Ping Yun 1,2 1 Sun Yat-sen University Cancer Center; State Key Laboratory of Oncology in South China; Collaborative Innovation Center for Cancer Medicine 2 Department of Pathology, Sun Yat-sen University Cancer Center * These authors contributed equally to this work Correspondence: Chris Zhiyi Zhang, email: // Jing-Ping Yun, email: // Keywords : miR-137; AKT2; FoxD3; metastasis; HCC Received : May 3, 2014 Accepted : June 9, 2014 Published : June 10, 2014 Abstract microRNAs, frequently deregulated in human cancer, have been implicated in the progression of hepatocarcinogenesis. Here, we show that microRNA (miR)-137 is significantly down-regulated in hepatocellular carcinoma (HCC). Its decreased expression is associated with vein invasion, incomplete Involucrum, and distant metastasis. Multivariate analysis suggests that miR-137 is an independent indicator for poor survival. We next show that over-expression of miR-137 suppresses cell proliferation, migration and invasion in vitro . Conversely, miR-137 inhibition promotes HCC cell growth. We also identify AKT2 as a key target of miR-137 in this context. Statistical data reveal a reverse correlation of AKT2 and miR-137 expression in HCC patients. Silencing of AKT2 phenotypically copied miR-137-induced phenotypes, whereas re-expression of AKT2 reversed the suppressive effects of miR-137. Further investigations showed that miR-137 exerted its anti-tumour activity via inhibiting the AKT2/mTOR pathway. Moreover, we demonstrate that FoxD3 directly binds to the promoter of miR-137 and activates its transcription. In vivo studies confirm that FoxD3-regulated miR-137 inhibited HCC growth and metastasis via targeting AKT2. Together, our findings indicate that miR-137 is a valuable biomarker for HCC prognosis and the FoxD3/miR-137/AKT2 regulatory network plays an important role in HCC progression.
Lili Liu - One of the best experts on this subject based on the ideXlab platform.
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abstract 3547 foxd3 regulated microrna 137 suppresses tumour growth and metastasis in human hepatocellular carcinoma by targeting akt2
Cancer Research, 2014Co-Authors: Lili Liu, Chris Zhiyi Zhang, Jingping YunAbstract:Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA Background and Aims: microRNAs, which are frequently deregulated in human cancer, have been implicated in the progression of hepatocarcinogenesis. Our study aims to investigate the role of miR-137 in cell proliferation and metastasis of hepatocellular carcinoma and the underlying molecular mechanisms. Methods: Real-time PCR and western blot were used to examine the expressions of miR-137, Akt2 and FoxD3 in HCC tissues and cells. The in vitro and in vivo effects of miR-137 were determined. Luciferase reporter assay was conducted to confirm the association of miR-137 and AKT2. CHIP assay was performed to test the regulation of miR-137 by FoxD3. Results: We show that microRNA (miR)-137 is significantly down-regulated in hepatocellular carcinoma (HCC), and its decreased expression is associated with vein invasion, incomplete Involucrum, and tumour metastasis. Multivariate analysis suggests that miR-137 is an independent indicator for poor survival. To determine its role in HCC development, we showed that overexpression of miR-137 suppresses cell proliferation, invasion and metastasis in vitro. Conversely, miR-137 inhibition promoted HCC cell growth. We also identified AKT2 as a key target of miR-137 in this context. Statistical data revealed a reverse correlation of AKT2 and miR-137 expression in HCC patients. Silencing of AKT2 phenotypically copied miR-137-induced phenotypes, whereas re-expression of AKT2 reversed the suppressive effects of miR-137. Further investigation showed that miR-137 exerted its anti-tumour activity via inhibiting the AKT2/mTOR pathway. Moreover, we demonstrated that FoxD3 binds to the promoter of miR-137 and activates its transcription. In vivo studies confirmed that FoxD3-regulated miR-137 inhibition of HCC growth and metastasis via directly targeting AKT2. Conclusions: Our findings indicated that miR-137 is a valuable biomarker for HCC prognosis and demonstrated that the FoxD3/miR-137/AKT2 regulatory network plays an important role in HCC progression. Citation Format: Li-Li Liu, Shi-Xun Lu, Min Li, Jia Fu, Chris Zhiyi Zhang, Jing-Ping Yun. FoxD3-regulated microRNA-137 suppresses tumour growth and metastasis in human hepatocellular carcinoma by targeting AKT2. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3547. doi:10.1158/1538-7445.AM2014-3547
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foxd3 regulated microrna 137 suppresses tumour growth and metastasis in human hepatocellular carcinoma by targeting akt2
Oncotarget, 2014Co-Authors: Lili Liu, Chris Zhiyi Zhang, Yuan Zhong Yang, Rong Zhen Luo, Jingping YunAbstract:// Li-Li Liu 1,2,* , Shi-Xun Lu 1,2,* , Min Li 1,2 , Lin-Zi Li 1,2 , Jia Fu 1,2 , Wen Hu 1,2 , Yuan-Zhong Yang 1,2 , Rong-Zhen Luo 1,2 , Chris Zhiyi Zhang 1,2 , Jing-Ping Yun 1,2 1 Sun Yat-sen University Cancer Center; State Key Laboratory of Oncology in South China; Collaborative Innovation Center for Cancer Medicine 2 Department of Pathology, Sun Yat-sen University Cancer Center * These authors contributed equally to this work Correspondence: Chris Zhiyi Zhang, email: // Jing-Ping Yun, email: // Keywords : miR-137; AKT2; FoxD3; metastasis; HCC Received : May 3, 2014 Accepted : June 9, 2014 Published : June 10, 2014 Abstract microRNAs, frequently deregulated in human cancer, have been implicated in the progression of hepatocarcinogenesis. Here, we show that microRNA (miR)-137 is significantly down-regulated in hepatocellular carcinoma (HCC). Its decreased expression is associated with vein invasion, incomplete Involucrum, and distant metastasis. Multivariate analysis suggests that miR-137 is an independent indicator for poor survival. We next show that over-expression of miR-137 suppresses cell proliferation, migration and invasion in vitro . Conversely, miR-137 inhibition promotes HCC cell growth. We also identify AKT2 as a key target of miR-137 in this context. Statistical data reveal a reverse correlation of AKT2 and miR-137 expression in HCC patients. Silencing of AKT2 phenotypically copied miR-137-induced phenotypes, whereas re-expression of AKT2 reversed the suppressive effects of miR-137. Further investigations showed that miR-137 exerted its anti-tumour activity via inhibiting the AKT2/mTOR pathway. Moreover, we demonstrate that FoxD3 directly binds to the promoter of miR-137 and activates its transcription. In vivo studies confirm that FoxD3-regulated miR-137 inhibited HCC growth and metastasis via targeting AKT2. Together, our findings indicate that miR-137 is a valuable biomarker for HCC prognosis and the FoxD3/miR-137/AKT2 regulatory network plays an important role in HCC progression.
Chris Zhiyi Zhang - One of the best experts on this subject based on the ideXlab platform.
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abstract 3547 foxd3 regulated microrna 137 suppresses tumour growth and metastasis in human hepatocellular carcinoma by targeting akt2
Cancer Research, 2014Co-Authors: Lili Liu, Chris Zhiyi Zhang, Jingping YunAbstract:Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA Background and Aims: microRNAs, which are frequently deregulated in human cancer, have been implicated in the progression of hepatocarcinogenesis. Our study aims to investigate the role of miR-137 in cell proliferation and metastasis of hepatocellular carcinoma and the underlying molecular mechanisms. Methods: Real-time PCR and western blot were used to examine the expressions of miR-137, Akt2 and FoxD3 in HCC tissues and cells. The in vitro and in vivo effects of miR-137 were determined. Luciferase reporter assay was conducted to confirm the association of miR-137 and AKT2. CHIP assay was performed to test the regulation of miR-137 by FoxD3. Results: We show that microRNA (miR)-137 is significantly down-regulated in hepatocellular carcinoma (HCC), and its decreased expression is associated with vein invasion, incomplete Involucrum, and tumour metastasis. Multivariate analysis suggests that miR-137 is an independent indicator for poor survival. To determine its role in HCC development, we showed that overexpression of miR-137 suppresses cell proliferation, invasion and metastasis in vitro. Conversely, miR-137 inhibition promoted HCC cell growth. We also identified AKT2 as a key target of miR-137 in this context. Statistical data revealed a reverse correlation of AKT2 and miR-137 expression in HCC patients. Silencing of AKT2 phenotypically copied miR-137-induced phenotypes, whereas re-expression of AKT2 reversed the suppressive effects of miR-137. Further investigation showed that miR-137 exerted its anti-tumour activity via inhibiting the AKT2/mTOR pathway. Moreover, we demonstrated that FoxD3 binds to the promoter of miR-137 and activates its transcription. In vivo studies confirmed that FoxD3-regulated miR-137 inhibition of HCC growth and metastasis via directly targeting AKT2. Conclusions: Our findings indicated that miR-137 is a valuable biomarker for HCC prognosis and demonstrated that the FoxD3/miR-137/AKT2 regulatory network plays an important role in HCC progression. Citation Format: Li-Li Liu, Shi-Xun Lu, Min Li, Jia Fu, Chris Zhiyi Zhang, Jing-Ping Yun. FoxD3-regulated microRNA-137 suppresses tumour growth and metastasis in human hepatocellular carcinoma by targeting AKT2. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3547. doi:10.1158/1538-7445.AM2014-3547
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foxd3 regulated microrna 137 suppresses tumour growth and metastasis in human hepatocellular carcinoma by targeting akt2
Oncotarget, 2014Co-Authors: Lili Liu, Chris Zhiyi Zhang, Yuan Zhong Yang, Rong Zhen Luo, Jingping YunAbstract:// Li-Li Liu 1,2,* , Shi-Xun Lu 1,2,* , Min Li 1,2 , Lin-Zi Li 1,2 , Jia Fu 1,2 , Wen Hu 1,2 , Yuan-Zhong Yang 1,2 , Rong-Zhen Luo 1,2 , Chris Zhiyi Zhang 1,2 , Jing-Ping Yun 1,2 1 Sun Yat-sen University Cancer Center; State Key Laboratory of Oncology in South China; Collaborative Innovation Center for Cancer Medicine 2 Department of Pathology, Sun Yat-sen University Cancer Center * These authors contributed equally to this work Correspondence: Chris Zhiyi Zhang, email: // Jing-Ping Yun, email: // Keywords : miR-137; AKT2; FoxD3; metastasis; HCC Received : May 3, 2014 Accepted : June 9, 2014 Published : June 10, 2014 Abstract microRNAs, frequently deregulated in human cancer, have been implicated in the progression of hepatocarcinogenesis. Here, we show that microRNA (miR)-137 is significantly down-regulated in hepatocellular carcinoma (HCC). Its decreased expression is associated with vein invasion, incomplete Involucrum, and distant metastasis. Multivariate analysis suggests that miR-137 is an independent indicator for poor survival. We next show that over-expression of miR-137 suppresses cell proliferation, migration and invasion in vitro . Conversely, miR-137 inhibition promotes HCC cell growth. We also identify AKT2 as a key target of miR-137 in this context. Statistical data reveal a reverse correlation of AKT2 and miR-137 expression in HCC patients. Silencing of AKT2 phenotypically copied miR-137-induced phenotypes, whereas re-expression of AKT2 reversed the suppressive effects of miR-137. Further investigations showed that miR-137 exerted its anti-tumour activity via inhibiting the AKT2/mTOR pathway. Moreover, we demonstrate that FoxD3 directly binds to the promoter of miR-137 and activates its transcription. In vivo studies confirm that FoxD3-regulated miR-137 inhibited HCC growth and metastasis via targeting AKT2. Together, our findings indicate that miR-137 is a valuable biomarker for HCC prognosis and the FoxD3/miR-137/AKT2 regulatory network plays an important role in HCC progression.
Luc Brendonck - One of the best experts on this subject based on the ideXlab platform.
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monophyly of the species of hepatozoon adeleorina hepatozoidae parasitizing african anurans with the description of three new species from hyperoliid frogs in south africa
Parasitology, 2017Co-Authors: Louis H Du Preez, Maarten P M Vanhove, Luc BrendonckAbstract:Haemogregarines (Apicomplexa: Adeleiorina) are a diverse group of haemoparasites reported from almost all vertebrate classes. The most commonly recorded haemogregarines to parasitize anurans are species of Hepatozoon Miller, 1908. To date 16 Hepatozoon species have been described from anurans in Africa, with only a single species, Hepatozoon hyperolli (Hoare, 1932), infecting a member of the Hyperoliidae. Furthermore, only two Hepatozoon species are known from South African anurans, namely Hepatozoon theileri (Laveran, 1905) and Hepatozoon ixoxo Netherlands, Cook and Smit, 2014, from Amietia delalandii (syn. Amietia quecketti) and three Sclerophrys species, respectively. Blood samples were collected from a total of 225 individuals representing nine hyperoliid species from several localities throughout northern KwaZulu-Natal, South Africa. Twenty frogs from three species were found positive for haemogregarines, namely Afrixalus fornasinii (6/14), Hyperolius argus (2/39), and Hyperolius marmoratus (12/74). Based on morphological characteristics, morphometrics and molecular findings three new haemogregarine species, Hepatozoon Involucrum Netherlands, Cook and Smit n. sp., Hepatozoon tenuis Netherlands, Cook and Smit n. sp. and Hepatozoon thori Netherlands, Cook and Smit n. sp., are described from hyperoliid hosts. Furthermore, molecular analyses show anuran Hepatozoon species to be a separate monophyletic group, with species isolated from African hosts forming a monophyletic clade within this cluster.
Satoshi Hozumi - One of the best experts on this subject based on the ideXlab platform.
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thermal characteristics of nests of the taiwanese stingless bee trigona ventralis hoozana hymenoptera apidae
Zoological Studies, 2008Co-Authors: Ihsin Sung, Soichi Yamane, Satoshi HozumiAbstract:Temperatures in 3 natural nests of the Taiwanese stingless bee Trigona (Lepidotrigona) ventralis hoozana were measured in Dapu and Tsaoshan, Chiayi County, Taiwan from Dec. 2002 to Mar. 2004. The temperature in the brood rearing zone of 2 nests was kept relatively constant in a range of 29-32℃. Even in the morning in Jan., the temperature in the brood area was maintained at 29.5℃, about 21℃ higher than the ambient temperature. Healthy nests could tolerate ambient temperatures even lower than 8℃, judging from the elevational distribution limit of this species and corresponding meteorological records. The maintenance of constant temperatures in the brood area is chiefly due to ameliorated thermal conditions in the nesting cavity. This is realized by the thermostatic effect of the thick wood of the living tree enclosing the cavity. The Involucrum, which completely encloses the brood combs, may retain thermal energy generated by the adults and partially by the mass of brood in the brood area.
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Thermal Characteristics of Nests of the Taiwanese Stingless Bee Trigona ventralis hoozana (Hymenoptera: Apidae)
2007Co-Authors: Ihsin Sung, Soichi Yamane, Satoshi HozumiAbstract:of nests of the Taiwanese stingless bee Trigona ventralis hoozana (Hymenoptera: Apidae). Zoological Studies 47(4): xxx-xxx. Temperatures in 3 natural nests of the Taiwanese stingless bee Trigona (Lepidotrigona) ventralis hoozana were measured in Dapu and Tsaoshan, Chiayi County, Taiwan from Dec. 2002 to Mar. 2004. The temperature in the brood rearing zone of 2 nests was kept relatively constant in a range of 29-32°C. Even in the morning in Jan., the temperature in the brood area was maintained at 29.5°C, about 21°C higher than the ambient temperature. Healthy nests could tolerate ambient temperatures even lower than 8°C, judging from the elevational distribution limit of this species and corresponding meteorological records. The maintenance of constant temperatures in the brood area is chiefly due to ameliorated thermal conditions in the nesting cavity. This is realized by the thermostatic effect of the thick wood of the living tree enclosing the cavity. The Involucrum, which completely encloses the brood combs, may retain thermal energy generated by the adults and partially by the mass of brood in the brood area
