The Experts below are selected from a list of 2532 Experts worldwide ranked by ideXlab platform
Manyun Zhang - One of the best experts on this subject based on the ideXlab platform.
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antagonistic effects of nitrification inhibitor 3 4 dimethylpyrazole phosphate and fungicide Iprodione on net nitrification in an agricultural soil
Soil Biology & Biochemistry, 2018Co-Authors: Manyun Zhang, Weijin Wang, Shahla Hosseini Bai, Xue Zhou, Ying TengAbstract:This study evaluated the effects of nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) and fungicide Iprodione on net nitrification rates and abundances of functional genes related to nitrification and denitrification in an agricultural soil. Single DMPP application or repeated Iprodione applications decreased net nitrification rates in the test soil. However, when the DMPP and Iprodione were applied together, they could generate antagonistic effects on the inhibitions of net nitrification rates. Repeated Iprodione applications reduced ammonia-oxidizing archaea and bacteria (AOA and AOB) amoA gene abundances, while DMPP application decreased AOB amoA gene abundances only. The abundances of narG and nirK genes were negatively affected by repeated Iprodione applications. Our results demonstrated that combined applications of DMPP and Iprodione could generate antagonistic effects on the inhibitions of net nitrification rates and discrepant impacts on the abundances of functional genes related to soil denitrification.
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effects of fungicide Iprodione and nitrification inhibitor 3 4 dimethylpyrazole phosphate on soil enzyme and bacterial properties
Science of The Total Environment, 2017Co-Authors: Manyun Zhang, Weijin Wang, Yaling Zhang, Ying TengAbstract:Agrochemical applications may have unintended detrimental effects on soil microorganisms and soil health. However, limited studies have been conducted to evaluate the effects of repeated fungicide applications and interactive effects of different agrochemical applications on soil microorganisms. In this study, an incubation experiment was established to evaluate the potential influences of the fungicide Iprodione and the nitrification inhibitor 3, 4-dimethylpyrazole phosphate (DMPP) on soil enzyme activities and bacterial properties. Weekly Iprodione applications decreased the activities of all enzymes tested, and DMPP application inhibited soil urease activity. Compared with the blank control, bacterial 16S rRNA gene abundance decreased following repeated Iprodione applications, but increased after DMPP application. After 28 days of incubation, the treatment receiving both Iprodione and DMPP application had higher bacterial 16S rRNA gene abundance and Shannon diversity index than the treatment with Iprodione applications alone. Repeated Iprodione applications significantly increased the relative abundance of Proteobacteria, but decreased the relative abundances of Chloroflexi and Acidobacteria. Simultaneously, bacterial community structure was changed by repeated Iprodione applications, alone or together with DMPP. These results showed that repeated Iprodione applications exerted negative effects on soil enzyme activities, bacterial biomass and community diversity. Moreover, relative to Iprodione applications alone, additional DMPP application could alleviate the toxic effects of Iprodione applications on bacterial biomass and community diversity.
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effects of nitrification inhibitor 3 4 dimethylpyrazole phosphate and fungicide Iprodione on soil fungal biomass and community based on internal transcribed spacer region
Journal of Soils and Sediments, 2017Co-Authors: Manyun Zhang, Yaling Zhang, Ying Teng, Rebecca FordAbstract:Nitrification inhibitors that impact soil nitrifying microorganisms have been widely applied in agricultural soils to enhance the efficiency of nitrogen fertilizers. However, little is known about their combined impact with other chemical applications, such as fungicides, on soil fungi. This study specifically examined the effects of the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP), alone or together with the fungicide Iprodione, on fungi biomass and community in a typical farmland soil. Four treatments were set: (1) control of zero agrochemical applications (CK), (2) a single DMPP application (DAA), (3) repeated Iprodione applications (4×IPR), and (4) combined applications of DMPP and Iprodione (DAA+4×IPR). The agrochemicals were applied at the recommended intervals, and the soil samples were incubated in the dark for 28 days. During the incubation, soil sample DNA was extracted, and the effects of DMPP and Iprodione applications on soil fungal internal transcribed spacer (ITS) abundances were determined with quantitative PCR (qPCR). At the end of the incubation, Illumina MiSeq method was employed to assess soil fungal community diversity and structure. DMPP application had a negligible effect on fungal ITS abundance. However, repeated Iprodione applications significantly decreased fungal ITS abundances. After 28 days of incubation, the fungal ITS abundances in the 4×IPR and DAA+4×IPR treatments were 43.6 and 56.2% of that measured from the CK treatment, respectively. Shannon indices of fungal communities demonstrated the treatment-induced gradients, with the DAA+4×IPR treatment harboring the highest Shannon index. Fungal community structures following the DAA and 4×IPR treatments remained overlapping with that in the CK treatment, but repeated Iprodione applications markedly enriched the family Teratosphaeriaceae. Relative to the CK treatment, fungal community structure in the DAA+4×IPR treatment was significantly changed, with the families Cephalothecaceae, Hypocreaceae, and Cordycipitaceae harboring a linear discriminant analysis value >3. DMPP application had negligible effects on soil fungal biomass, community diversity, and structure, potentially indicating that the DMPP is “bio-safe.” Conversely, repeated Iprodione applications significantly decreased fungal ITS abundances. Moreover, the family Teratosphaeriaceae could be further investigated as a potential biomarker of the impacts of Iprodione on soil fungi. The combined applications of DMPP and Iprodione stimulated the Shannon diversity index and markedly changed soil fungal community structure.
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dynamics of biochemical properties associated with soil nitrogen mineralization following nitrification inhibitor and fungicide applications
Environmental Science and Pollution Research, 2017Co-Authors: Manyun Zhang, Weijin Wang, Jun Wang, Ying TengAbstract:Agrochemical applications may have side effects on soil biochemical properties related to soil nitrogen (N) mineralization and thus affect N cycling. The present study aimed to evaluate the effects of nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) and fungicide Iprodione on soil neutral protease (NPR), alkaline protease (APR), chitinase (CHI), and their functional genes (nprA, aprA, and chiA) related to soil N mineralization. The following four treatments were included: blank control (CK), single DMPP application (DAA), weekly Iprodione applications (IPR), and the combined applications of DMPP and Iprodione (DI). Compared with the CK treatment, DMPP application significantly inhibited the CHI activity in the first 14 days of incubation, and Iprodione applications, particularly when applied alone, decreased the NPR, APR, and CHI activities. Relative to the IPR treatment, extra DMPP application had the potential to alleviate the inhibitory effects of Iprodione on the activities of these enzymes. DMPP application significantly increased aprA gene abundances after 14 days of incubation. However, repeated Iprodione applications, alone or with the DMPP, decreased nprA and chiA gene abundances. Relative to the CK treatment, DMPP application generated negligible effects on the positive/negative correlations between soil enzyme activities and the corresponding functional gene abundances. However, the positive correlation between the CHI activity and chiA gene abundance was changed to negative correlation by repeated Iprodione applications, alone or together with the DMPP. Our results demonstrated that agrochemical applications, particularly repeated fungicide applications, can have inadvertent effects on enzyme activities and functional gene abundances associated with soil N mineralization.
Ying Teng - One of the best experts on this subject based on the ideXlab platform.
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antagonistic effects of nitrification inhibitor 3 4 dimethylpyrazole phosphate and fungicide Iprodione on net nitrification in an agricultural soil
Soil Biology & Biochemistry, 2018Co-Authors: Manyun Zhang, Weijin Wang, Shahla Hosseini Bai, Xue Zhou, Ying TengAbstract:This study evaluated the effects of nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) and fungicide Iprodione on net nitrification rates and abundances of functional genes related to nitrification and denitrification in an agricultural soil. Single DMPP application or repeated Iprodione applications decreased net nitrification rates in the test soil. However, when the DMPP and Iprodione were applied together, they could generate antagonistic effects on the inhibitions of net nitrification rates. Repeated Iprodione applications reduced ammonia-oxidizing archaea and bacteria (AOA and AOB) amoA gene abundances, while DMPP application decreased AOB amoA gene abundances only. The abundances of narG and nirK genes were negatively affected by repeated Iprodione applications. Our results demonstrated that combined applications of DMPP and Iprodione could generate antagonistic effects on the inhibitions of net nitrification rates and discrepant impacts on the abundances of functional genes related to soil denitrification.
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effects of fungicide Iprodione and nitrification inhibitor 3 4 dimethylpyrazole phosphate on soil enzyme and bacterial properties
Science of The Total Environment, 2017Co-Authors: Manyun Zhang, Weijin Wang, Yaling Zhang, Ying TengAbstract:Agrochemical applications may have unintended detrimental effects on soil microorganisms and soil health. However, limited studies have been conducted to evaluate the effects of repeated fungicide applications and interactive effects of different agrochemical applications on soil microorganisms. In this study, an incubation experiment was established to evaluate the potential influences of the fungicide Iprodione and the nitrification inhibitor 3, 4-dimethylpyrazole phosphate (DMPP) on soil enzyme activities and bacterial properties. Weekly Iprodione applications decreased the activities of all enzymes tested, and DMPP application inhibited soil urease activity. Compared with the blank control, bacterial 16S rRNA gene abundance decreased following repeated Iprodione applications, but increased after DMPP application. After 28 days of incubation, the treatment receiving both Iprodione and DMPP application had higher bacterial 16S rRNA gene abundance and Shannon diversity index than the treatment with Iprodione applications alone. Repeated Iprodione applications significantly increased the relative abundance of Proteobacteria, but decreased the relative abundances of Chloroflexi and Acidobacteria. Simultaneously, bacterial community structure was changed by repeated Iprodione applications, alone or together with DMPP. These results showed that repeated Iprodione applications exerted negative effects on soil enzyme activities, bacterial biomass and community diversity. Moreover, relative to Iprodione applications alone, additional DMPP application could alleviate the toxic effects of Iprodione applications on bacterial biomass and community diversity.
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effects of nitrification inhibitor 3 4 dimethylpyrazole phosphate and fungicide Iprodione on soil fungal biomass and community based on internal transcribed spacer region
Journal of Soils and Sediments, 2017Co-Authors: Manyun Zhang, Yaling Zhang, Ying Teng, Rebecca FordAbstract:Nitrification inhibitors that impact soil nitrifying microorganisms have been widely applied in agricultural soils to enhance the efficiency of nitrogen fertilizers. However, little is known about their combined impact with other chemical applications, such as fungicides, on soil fungi. This study specifically examined the effects of the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP), alone or together with the fungicide Iprodione, on fungi biomass and community in a typical farmland soil. Four treatments were set: (1) control of zero agrochemical applications (CK), (2) a single DMPP application (DAA), (3) repeated Iprodione applications (4×IPR), and (4) combined applications of DMPP and Iprodione (DAA+4×IPR). The agrochemicals were applied at the recommended intervals, and the soil samples were incubated in the dark for 28 days. During the incubation, soil sample DNA was extracted, and the effects of DMPP and Iprodione applications on soil fungal internal transcribed spacer (ITS) abundances were determined with quantitative PCR (qPCR). At the end of the incubation, Illumina MiSeq method was employed to assess soil fungal community diversity and structure. DMPP application had a negligible effect on fungal ITS abundance. However, repeated Iprodione applications significantly decreased fungal ITS abundances. After 28 days of incubation, the fungal ITS abundances in the 4×IPR and DAA+4×IPR treatments were 43.6 and 56.2% of that measured from the CK treatment, respectively. Shannon indices of fungal communities demonstrated the treatment-induced gradients, with the DAA+4×IPR treatment harboring the highest Shannon index. Fungal community structures following the DAA and 4×IPR treatments remained overlapping with that in the CK treatment, but repeated Iprodione applications markedly enriched the family Teratosphaeriaceae. Relative to the CK treatment, fungal community structure in the DAA+4×IPR treatment was significantly changed, with the families Cephalothecaceae, Hypocreaceae, and Cordycipitaceae harboring a linear discriminant analysis value >3. DMPP application had negligible effects on soil fungal biomass, community diversity, and structure, potentially indicating that the DMPP is “bio-safe.” Conversely, repeated Iprodione applications significantly decreased fungal ITS abundances. Moreover, the family Teratosphaeriaceae could be further investigated as a potential biomarker of the impacts of Iprodione on soil fungi. The combined applications of DMPP and Iprodione stimulated the Shannon diversity index and markedly changed soil fungal community structure.
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dynamics of biochemical properties associated with soil nitrogen mineralization following nitrification inhibitor and fungicide applications
Environmental Science and Pollution Research, 2017Co-Authors: Manyun Zhang, Weijin Wang, Jun Wang, Ying TengAbstract:Agrochemical applications may have side effects on soil biochemical properties related to soil nitrogen (N) mineralization and thus affect N cycling. The present study aimed to evaluate the effects of nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) and fungicide Iprodione on soil neutral protease (NPR), alkaline protease (APR), chitinase (CHI), and their functional genes (nprA, aprA, and chiA) related to soil N mineralization. The following four treatments were included: blank control (CK), single DMPP application (DAA), weekly Iprodione applications (IPR), and the combined applications of DMPP and Iprodione (DI). Compared with the CK treatment, DMPP application significantly inhibited the CHI activity in the first 14 days of incubation, and Iprodione applications, particularly when applied alone, decreased the NPR, APR, and CHI activities. Relative to the IPR treatment, extra DMPP application had the potential to alleviate the inhibitory effects of Iprodione on the activities of these enzymes. DMPP application significantly increased aprA gene abundances after 14 days of incubation. However, repeated Iprodione applications, alone or with the DMPP, decreased nprA and chiA gene abundances. Relative to the CK treatment, DMPP application generated negligible effects on the positive/negative correlations between soil enzyme activities and the corresponding functional gene abundances. However, the positive correlation between the CHI activity and chiA gene abundance was changed to negative correlation by repeated Iprodione applications, alone or together with the DMPP. Our results demonstrated that agrochemical applications, particularly repeated fungicide applications, can have inadvertent effects on enzyme activities and functional gene abundances associated with soil N mineralization.
Philippe Cayot - One of the best experts on this subject based on the ideXlab platform.
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Molecularly imprinted sol-gel polymers for the analysis of Iprodione fungicide in wine: Synthesis in green solvent
Food Chemistry, 2019Co-Authors: Manal Bitar, Philippe Cayot, Céline Lafarge, Nicolas Sok, Elias Bou-marounAbstract:Iprodione is a fungicide widely used in viticulture in most agricultural countries. It was banned recently in the European community because of its carcinogenic and endocrine disrupting characters. In this work, a cheap analytical method able to monitor Iprodione in a white wine was developed. Molecularly imprinted sol-gel polymers (MIS) specific to Iprodione and using green solvents were synthesized. An experimental design having the following factors (solvent volume and crosslinker quantity) was used to prepare an optimal MIS. In terms of selectivity, the optimal MIS showed the best partition coefficient towards Iprodione in a white wine containing four other competing fungicides (procymidone, pyrimethanil, azoxystrobin and iprovalicarb). A solid phase extraction method using the optimal MIS was optimized and applied to analyse Iprodione in a white wine. Low detection and quantification limits were reached 11.7 and 39.1 µg/L respectively.
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Feasibility of a microwave liquid sensor based on molecularly imprinted sol-gel polymer for the detection of Iprodione fungicide
Sensors and Actuators B: Chemical, 2017Co-Authors: Elias Bou-maroun, Jérôme Rossignol, Brice De Fonseca, Céline Lafarge, Régis D. Gougeon, Didier Stuerga, Philippe CayotAbstract:A new microwave sensor coupled with molecularly imprinted silica is described here for the first time in order to detect Iprodione fungicide in hydroalcoholic medium. A Molecularly Imprinted Silica (MIS) and its control (Non-imprinted silica, NIS) were synthesized and characterized by infrared spectroscopy and scanning electron microscopy. The capacity of the MIS was determined by batch binding studies. MIS showed aggregates on micrometric scale and had a good capacity of sorption towards Iprodione of 25 mg/g, whereas the NIS adsorption of Iprodione was insignificant. MIS and NIS were deposited on the surface of the sensor by spin coating. The MIS and NIS microwave sensors were then tested in hydroalcoholic medium containing Iprodione. The sensor response was stable over 10 min. The response of the MIS microwave senor was dependent on Iprodione concentration whereas, the response of the NIS microwave sensor was not. The lowest detected Iprodione concentration was 10−9 mol/L or 0.33 ng/L of Iprodione.
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experimental design approach in the synthesis of molecularly imprinted polymers specific for Iprodione fungicide
Reactive & Functional Polymers, 2015Co-Authors: Philippe Cayot, Manal Bitar, Jacqueline Maalouly, Hanna Chebib, Adrien Lerbret, Elias BoumarounAbstract:Abstract An experimental design (ED) approach was applied to study the weight of three factors in the synthesis of a molecularly imprinted polymer (MIP) specific for Iprodione fungicide. The objective was to obtain a high specific polymer with the best performance of Iprodione binding. Thirteen Iprodione-imprinted polymers and 13 non-imprinted polymers (NIP) were synthesized according to ED having 3 influencing factors: the polymerization method, the crosslinker nature and the functional monomer type. For each factor, two levels were studied: bulk and precipitation polymerization for the first factor, trimethylolpropane trimethacrylate (TRIM) and ethylene glycol dimethacrylate (EGDMA) for the second factor, and methacrylamide (MAM) alone or with styrene for the third factor. The ED responses were: the imprinting factor, the site number and the apparent affinity constant. They were determined after studying the interactions between Iprodione and each MIP and NIP in hydro-alcoholic medium. The best polymer of the ED was synthesized using MAM as functional monomer, EGDMA as crosslinker and precipitation polymerization. It has an imprinting factor of 2.4, a site number of 1172 and an affinity constant of 19.36. The best MIP was characterized by Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM) and Nitrogen sorption isotherms. The best MIP has a mesoporous structure with a high specific surface area of 407 m 2 ·g − 1 . Molecularly imprinted solid phase extraction (MISPE) was successfully applied, using the best MIP, to preconcentrate Iprodione from a white wine containing two competing fungicides.
Manal Bitar - One of the best experts on this subject based on the ideXlab platform.
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Molecularly imprinted sol-gel polymers for the analysis of Iprodione fungicide in wine: Synthesis in green solvent
Food Chemistry, 2019Co-Authors: Manal Bitar, Philippe Cayot, Céline Lafarge, Nicolas Sok, Elias Bou-marounAbstract:Iprodione is a fungicide widely used in viticulture in most agricultural countries. It was banned recently in the European community because of its carcinogenic and endocrine disrupting characters. In this work, a cheap analytical method able to monitor Iprodione in a white wine was developed. Molecularly imprinted sol-gel polymers (MIS) specific to Iprodione and using green solvents were synthesized. An experimental design having the following factors (solvent volume and crosslinker quantity) was used to prepare an optimal MIS. In terms of selectivity, the optimal MIS showed the best partition coefficient towards Iprodione in a white wine containing four other competing fungicides (procymidone, pyrimethanil, azoxystrobin and iprovalicarb). A solid phase extraction method using the optimal MIS was optimized and applied to analyse Iprodione in a white wine. Low detection and quantification limits were reached 11.7 and 39.1 µg/L respectively.
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experimental design approach in the synthesis of molecularly imprinted polymers specific for Iprodione fungicide
Reactive & Functional Polymers, 2015Co-Authors: Philippe Cayot, Manal Bitar, Jacqueline Maalouly, Hanna Chebib, Adrien Lerbret, Elias BoumarounAbstract:Abstract An experimental design (ED) approach was applied to study the weight of three factors in the synthesis of a molecularly imprinted polymer (MIP) specific for Iprodione fungicide. The objective was to obtain a high specific polymer with the best performance of Iprodione binding. Thirteen Iprodione-imprinted polymers and 13 non-imprinted polymers (NIP) were synthesized according to ED having 3 influencing factors: the polymerization method, the crosslinker nature and the functional monomer type. For each factor, two levels were studied: bulk and precipitation polymerization for the first factor, trimethylolpropane trimethacrylate (TRIM) and ethylene glycol dimethacrylate (EGDMA) for the second factor, and methacrylamide (MAM) alone or with styrene for the third factor. The ED responses were: the imprinting factor, the site number and the apparent affinity constant. They were determined after studying the interactions between Iprodione and each MIP and NIP in hydro-alcoholic medium. The best polymer of the ED was synthesized using MAM as functional monomer, EGDMA as crosslinker and precipitation polymerization. It has an imprinting factor of 2.4, a site number of 1172 and an affinity constant of 19.36. The best MIP was characterized by Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM) and Nitrogen sorption isotherms. The best MIP has a mesoporous structure with a high specific surface area of 407 m 2 ·g − 1 . Molecularly imprinted solid phase extraction (MISPE) was successfully applied, using the best MIP, to preconcentrate Iprodione from a white wine containing two competing fungicides.
Elias Bou-maroun - One of the best experts on this subject based on the ideXlab platform.
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Molecularly imprinted sol-gel polymers for the analysis of Iprodione fungicide in wine: Synthesis in green solvent
Food Chemistry, 2019Co-Authors: Manal Bitar, Philippe Cayot, Céline Lafarge, Nicolas Sok, Elias Bou-marounAbstract:Iprodione is a fungicide widely used in viticulture in most agricultural countries. It was banned recently in the European community because of its carcinogenic and endocrine disrupting characters. In this work, a cheap analytical method able to monitor Iprodione in a white wine was developed. Molecularly imprinted sol-gel polymers (MIS) specific to Iprodione and using green solvents were synthesized. An experimental design having the following factors (solvent volume and crosslinker quantity) was used to prepare an optimal MIS. In terms of selectivity, the optimal MIS showed the best partition coefficient towards Iprodione in a white wine containing four other competing fungicides (procymidone, pyrimethanil, azoxystrobin and iprovalicarb). A solid phase extraction method using the optimal MIS was optimized and applied to analyse Iprodione in a white wine. Low detection and quantification limits were reached 11.7 and 39.1 µg/L respectively.
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Feasibility of a microwave liquid sensor based on molecularly imprinted sol-gel polymer for the detection of Iprodione fungicide
Sensors and Actuators B: Chemical, 2017Co-Authors: Elias Bou-maroun, Jérôme Rossignol, Brice De Fonseca, Céline Lafarge, Régis D. Gougeon, Didier Stuerga, Philippe CayotAbstract:A new microwave sensor coupled with molecularly imprinted silica is described here for the first time in order to detect Iprodione fungicide in hydroalcoholic medium. A Molecularly Imprinted Silica (MIS) and its control (Non-imprinted silica, NIS) were synthesized and characterized by infrared spectroscopy and scanning electron microscopy. The capacity of the MIS was determined by batch binding studies. MIS showed aggregates on micrometric scale and had a good capacity of sorption towards Iprodione of 25 mg/g, whereas the NIS adsorption of Iprodione was insignificant. MIS and NIS were deposited on the surface of the sensor by spin coating. The MIS and NIS microwave sensors were then tested in hydroalcoholic medium containing Iprodione. The sensor response was stable over 10 min. The response of the MIS microwave senor was dependent on Iprodione concentration whereas, the response of the NIS microwave sensor was not. The lowest detected Iprodione concentration was 10−9 mol/L or 0.33 ng/L of Iprodione.