The Experts below are selected from a list of 63 Experts worldwide ranked by ideXlab platform
Ye Liu - One of the best experts on this subject based on the ideXlab platform.
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R (2013) Isolation of Irkut Virus from a Murina leucogaster bat
2016Co-Authors: Ye Liu, Shou Feng Zhang, Jinghui Zhao, Fei ZhangAbstract:Background and objectives: Bats are recognized as a major reservoir of lyssaViruses; however, no bat lyssaVirus has been isolated in Asia except for Aravan and Khujand Virus in Central Asia. All Chinese lyssaVirus isolates in previous reports have been of species rabies Virus, mainly from dogs. Following at least two recent bat-associated human rabies-like cases in northeast China, we have initiated a study of the prevalence of lyssaViruses in bats in Jilin province and their public health implications. A bat lyssaVirus has been isolated and its pathogenicity in mice and genomic alignment have been determined. Results: We report the first isolation of a bat lyssaVirus in China, from the brain of a northeastern bat, Murina leucogaster. Its nucleoprotein gene shared 92.4%/98.9 % (nucleotide) and 92.2%/98.8 % (amino acid) identity with the two known Irkut Virus isolates from Russia, and was designated IRKV-THChina12. Following intracranial and intramuscular injection, IRKV-THChina12 produced rabies-like symptoms in adult mice with a short inoculation period and high mortality. Nucleotide sequence analysis showed that IRKV-THChina12 has the same genomic organization as other lyssaViruses and its isolation provides an independent origin for the species IRKV. Conclusions: We have identified the existence of a bat lyssaVirus in a common Chinese bat species. Its high pathogenicity in adult mice suggests that public warnings and medical education regarding bat bites in China should be increased, and tha
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Maximum likelihood phylogenetic tree based on the complete rabies Virus nucleoprotein gene.
2016Co-Authors: Ye Liu, Fei Zhang, Shou Feng Zhang, He-ping Zhang, Jin-xiang Wang, Hai-ning Zhou, Yu-mei WangAbstract:The tree is rooted with Irkut Virus isolate JX442979, European bat lyssaVirus type 1 and 2 isolates EU293109 and Eu293114. The RABV isolates in this study are marked using black triangles, black squares and black circles.
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construction of a recombinant replication defective human adenoVirus type 5 expressing g protein of Irkut Virus and the immune test in mouse
Chinese journal of virology, 2015Co-Authors: Yuying Wang, Ye Liu, Qi Chen, Lecui ZhangAbstract:To develop a safe and effective new generation vaccine for IRKV-THChina12 prevention, we constructed a non-replicative recombinant human adenoVirus carrying the IRKV-THChina12 G gene, named as rAd5-IRKV-G. The IRKV-THChina12 G protein expressed by the recombinant human adenoVirus in 293AD cells was detected by western blot and indirect immunofluorescence test. To evaluate the immunogenicity of the recombinant, mice were immunized with rAd5-IRKV-G by intramuscular (i. m.) or intraperitoneal (i. p.) route and with non-exogenous gene expressing wild type adenoVirus wt-rAd5 as a control. Results showed that the rAd5-IRKV-G could induce continuous and statistically significant (P ≤ 0.05) anti-IRKV neutralizing antibody (NA) production in immunized mice by i. m. or i. p. route. In particular, no significant difference (P > 0.05) of the NA titers between the two administration routes were observed, that provides an alternative choice for animal immunization method in the future application.
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early diagnosis of Irkut Virus infection using magnetic bead based serum peptide profiling by maldi tof ms in a mouse model
International Journal of Molecular Sciences, 2014Co-Authors: Ye Liu, Zhuo Hao, Shou Feng ZhangAbstract:Early diagnosis is important for the prompt post-exposure prophylaxis of lyssaVirus infections. To diagnose Irkut Virus (IRKV) infection during incubation in mice, a novel method using magnetic bead-based serum peptide profiling by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been established. For this test, serum peptides were concentrated by adsorption to and elution from the magnetic bead-based weak cation ion exchanger. Mass spectrograms obtained by MALDI-TOF MS were analyzed using ClinProTools bioinformatics software. Construction of the diagnostic model was performed using serum samples from mice infected with IRKV and rabies Virus (RABV) BD06, Flury-LEP, and SRV9 (as controls). The method accurately diagnosed sera 2, 4 and 8 days after IRKV and RABV infections. The sensitivity, specificity, and total accuracy of diagnosis were 86.7%, 95.2%, and 92.9%, respectively. However, IRKV could not be differentiated from RABV 1 day after infection. The results of the present study indicate that serum peptide profiling by MALDI-TOF MS is a promising technique for the early clinical diagnosis of lyssaVirus infections and needs to be further tested in humans and farm animals.
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analysis of the complete genome of the first Irkut Virus isolate from china comparison across the lyssaVirus genus
Molecular Phylogenetics and Evolution, 2013Co-Authors: Ye Liu, Fei Zhang, Shou Feng Zhang, Hai Lian, Ying Wang, Jinxia ZhangAbstract:The genome of Irkut Virus, isolate IRKV-THChina12, the first non-rabies lyssaVirus from China (of bat origin), has been completely sequenced. In general, coding and non-coding regions of this viral genome are similar to those of other lyssaViruses. However, alignment of the deduced amino acid sequences of the structural proteins of IRKV-THChina12 with those of other lyssaVirus representatives revealed significant variability between viral species. The nucleoprotein and matrix protein were found to be the most conserved, followed by the large protein, glycoprotein and phosphoprotein. Differences in the antigenic sites in glycoprotein may result in only partial protection of the available rabies biologics against Irkut Virus, which is of particular concern for pre- and post-exposure rabies prophylaxis.
Shou Feng Zhang - One of the best experts on this subject based on the ideXlab platform.
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Possible Transmission of Irkut Virus from Dogs to Humans.
Biomedical and Environmental Sciences, 2018Co-Authors: Teng Chen, Fa Ming Miao, Nan Li, Fei Zhang, Shou Feng Zhang, Rong Liang HuAbstract:LyssaViruses, including Rabies Virus, Duvenhage Virus, European bat lyssaVirus 1, European bat lyssaVirus 2, Australian bat lyssaVirus, and Irkut Virus (IRKV), have caused human fatalities, but infection of IRKV in dogs has not been previously reported. In China, a dead dog that previously bit a human was determined to be infected with IRKV. Pathogenicity tests revealed that IRKVs can cause rabies-like disease in dogs and cats after laboratory infection. The close relationship between humans and pets, such as dogs and cats, may generate a new spillover-spreading route for IRKV infection. Therefore, additional attention should be paid to trans-species infection of IRKV between bats and dogs or dogs and humans through investigation of the prevalence and circulation patterns of IRKV in China.
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R (2013) Isolation of Irkut Virus from a Murina leucogaster bat
2016Co-Authors: Ye Liu, Shou Feng Zhang, Jinghui Zhao, Fei ZhangAbstract:Background and objectives: Bats are recognized as a major reservoir of lyssaViruses; however, no bat lyssaVirus has been isolated in Asia except for Aravan and Khujand Virus in Central Asia. All Chinese lyssaVirus isolates in previous reports have been of species rabies Virus, mainly from dogs. Following at least two recent bat-associated human rabies-like cases in northeast China, we have initiated a study of the prevalence of lyssaViruses in bats in Jilin province and their public health implications. A bat lyssaVirus has been isolated and its pathogenicity in mice and genomic alignment have been determined. Results: We report the first isolation of a bat lyssaVirus in China, from the brain of a northeastern bat, Murina leucogaster. Its nucleoprotein gene shared 92.4%/98.9 % (nucleotide) and 92.2%/98.8 % (amino acid) identity with the two known Irkut Virus isolates from Russia, and was designated IRKV-THChina12. Following intracranial and intramuscular injection, IRKV-THChina12 produced rabies-like symptoms in adult mice with a short inoculation period and high mortality. Nucleotide sequence analysis showed that IRKV-THChina12 has the same genomic organization as other lyssaViruses and its isolation provides an independent origin for the species IRKV. Conclusions: We have identified the existence of a bat lyssaVirus in a common Chinese bat species. Its high pathogenicity in adult mice suggests that public warnings and medical education regarding bat bites in China should be increased, and tha
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Maximum likelihood phylogenetic tree based on the complete rabies Virus nucleoprotein gene.
2016Co-Authors: Ye Liu, Fei Zhang, Shou Feng Zhang, He-ping Zhang, Jin-xiang Wang, Hai-ning Zhou, Yu-mei WangAbstract:The tree is rooted with Irkut Virus isolate JX442979, European bat lyssaVirus type 1 and 2 isolates EU293109 and Eu293114. The RABV isolates in this study are marked using black triangles, black squares and black circles.
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early diagnosis of Irkut Virus infection using magnetic bead based serum peptide profiling by maldi tof ms in a mouse model
International Journal of Molecular Sciences, 2014Co-Authors: Ye Liu, Zhuo Hao, Shou Feng ZhangAbstract:Early diagnosis is important for the prompt post-exposure prophylaxis of lyssaVirus infections. To diagnose Irkut Virus (IRKV) infection during incubation in mice, a novel method using magnetic bead-based serum peptide profiling by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been established. For this test, serum peptides were concentrated by adsorption to and elution from the magnetic bead-based weak cation ion exchanger. Mass spectrograms obtained by MALDI-TOF MS were analyzed using ClinProTools bioinformatics software. Construction of the diagnostic model was performed using serum samples from mice infected with IRKV and rabies Virus (RABV) BD06, Flury-LEP, and SRV9 (as controls). The method accurately diagnosed sera 2, 4 and 8 days after IRKV and RABV infections. The sensitivity, specificity, and total accuracy of diagnosis were 86.7%, 95.2%, and 92.9%, respectively. However, IRKV could not be differentiated from RABV 1 day after infection. The results of the present study indicate that serum peptide profiling by MALDI-TOF MS is a promising technique for the early clinical diagnosis of lyssaVirus infections and needs to be further tested in humans and farm animals.
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analysis of the complete genome of the first Irkut Virus isolate from china comparison across the lyssaVirus genus
Molecular Phylogenetics and Evolution, 2013Co-Authors: Ye Liu, Fei Zhang, Shou Feng Zhang, Hai Lian, Ying Wang, Jinxia ZhangAbstract:The genome of Irkut Virus, isolate IRKV-THChina12, the first non-rabies lyssaVirus from China (of bat origin), has been completely sequenced. In general, coding and non-coding regions of this viral genome are similar to those of other lyssaViruses. However, alignment of the deduced amino acid sequences of the structural proteins of IRKV-THChina12 with those of other lyssaVirus representatives revealed significant variability between viral species. The nucleoprotein and matrix protein were found to be the most conserved, followed by the large protein, glycoprotein and phosphoprotein. Differences in the antigenic sites in glycoprotein may result in only partial protection of the available rabies biologics against Irkut Virus, which is of particular concern for pre- and post-exposure rabies prophylaxis.
Fei Zhang - One of the best experts on this subject based on the ideXlab platform.
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Possible Transmission of Irkut Virus from Dogs to Humans.
Biomedical and Environmental Sciences, 2018Co-Authors: Teng Chen, Fa Ming Miao, Nan Li, Fei Zhang, Shou Feng Zhang, Rong Liang HuAbstract:LyssaViruses, including Rabies Virus, Duvenhage Virus, European bat lyssaVirus 1, European bat lyssaVirus 2, Australian bat lyssaVirus, and Irkut Virus (IRKV), have caused human fatalities, but infection of IRKV in dogs has not been previously reported. In China, a dead dog that previously bit a human was determined to be infected with IRKV. Pathogenicity tests revealed that IRKVs can cause rabies-like disease in dogs and cats after laboratory infection. The close relationship between humans and pets, such as dogs and cats, may generate a new spillover-spreading route for IRKV infection. Therefore, additional attention should be paid to trans-species infection of IRKV between bats and dogs or dogs and humans through investigation of the prevalence and circulation patterns of IRKV in China.
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R (2013) Isolation of Irkut Virus from a Murina leucogaster bat
2016Co-Authors: Ye Liu, Shou Feng Zhang, Jinghui Zhao, Fei ZhangAbstract:Background and objectives: Bats are recognized as a major reservoir of lyssaViruses; however, no bat lyssaVirus has been isolated in Asia except for Aravan and Khujand Virus in Central Asia. All Chinese lyssaVirus isolates in previous reports have been of species rabies Virus, mainly from dogs. Following at least two recent bat-associated human rabies-like cases in northeast China, we have initiated a study of the prevalence of lyssaViruses in bats in Jilin province and their public health implications. A bat lyssaVirus has been isolated and its pathogenicity in mice and genomic alignment have been determined. Results: We report the first isolation of a bat lyssaVirus in China, from the brain of a northeastern bat, Murina leucogaster. Its nucleoprotein gene shared 92.4%/98.9 % (nucleotide) and 92.2%/98.8 % (amino acid) identity with the two known Irkut Virus isolates from Russia, and was designated IRKV-THChina12. Following intracranial and intramuscular injection, IRKV-THChina12 produced rabies-like symptoms in adult mice with a short inoculation period and high mortality. Nucleotide sequence analysis showed that IRKV-THChina12 has the same genomic organization as other lyssaViruses and its isolation provides an independent origin for the species IRKV. Conclusions: We have identified the existence of a bat lyssaVirus in a common Chinese bat species. Its high pathogenicity in adult mice suggests that public warnings and medical education regarding bat bites in China should be increased, and tha
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Maximum likelihood phylogenetic tree based on the complete rabies Virus nucleoprotein gene.
2016Co-Authors: Ye Liu, Fei Zhang, Shou Feng Zhang, He-ping Zhang, Jin-xiang Wang, Hai-ning Zhou, Yu-mei WangAbstract:The tree is rooted with Irkut Virus isolate JX442979, European bat lyssaVirus type 1 and 2 isolates EU293109 and Eu293114. The RABV isolates in this study are marked using black triangles, black squares and black circles.
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analysis of the complete genome of the first Irkut Virus isolate from china comparison across the lyssaVirus genus
Molecular Phylogenetics and Evolution, 2013Co-Authors: Ye Liu, Fei Zhang, Shou Feng Zhang, Hai Lian, Ying Wang, Jinxia ZhangAbstract:The genome of Irkut Virus, isolate IRKV-THChina12, the first non-rabies lyssaVirus from China (of bat origin), has been completely sequenced. In general, coding and non-coding regions of this viral genome are similar to those of other lyssaViruses. However, alignment of the deduced amino acid sequences of the structural proteins of IRKV-THChina12 with those of other lyssaVirus representatives revealed significant variability between viral species. The nucleoprotein and matrix protein were found to be the most conserved, followed by the large protein, glycoprotein and phosphoprotein. Differences in the antigenic sites in glycoprotein may result in only partial protection of the available rabies biologics against Irkut Virus, which is of particular concern for pre- and post-exposure rabies prophylaxis.
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evaluation of rabies biologics against Irkut Virus isolated in china
Journal of Clinical Microbiology, 2013Co-Authors: Ye Liu, Fei Zhang, Shou Feng Zhang, Hai Lian, Ying Wang, Qi Chen, Jinxia ZhangAbstract:An Irkut Virus (IRKV) was recently isolated from a bat in China. The protective ability of rabies biologics available in the Chinese market and experimental biologics against the rabies Virus (RABV) and IRKV were assessed in a hamster model via preexposure prophylaxis (PrEP) and postexposure prophylaxis (PEP) experiments. The results demonstrated that a single dose of rabies vaccine did not induce adequate protection against IRKV infection. However, routine PrEP with three doses of vaccine induced complete protection against IRKV infection. Higher doses of RABV immunoglobulins and alpha interferon were required during PEP to protect hamsters against IRKV versus RABV infection. Experimental recombinant vaccines containing IRKV glycoproteins induced more-reliable protection against IRKV than against RABV infection. Those findings may be explained by limited cross-neutralization of these Viruses (confirmed via in vitro tests) in conjunction with antigenic distances between RABV and IRKV. These results indicate that the development and evaluation of new biologics for PrEP and PEP are required to ensure sufficient protection against IRKV infection in China and other territories where this Virus is present.
Gareth J Hughes - One of the best experts on this subject based on the ideXlab platform.
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experimental infection of big brown bats eptesicus fuscus with eurasian bat lyssaViruses aravan khujand and Irkut Virus
Archives of Virology, 2006Co-Authors: Gareth J Hughes, A Schmitz, Jesse D Blanton, Jamie Manangan, Staci M MurphyAbstract:Here we describe the results of experimental infections of captive big brown bats (Eptesicus fuscus) with three newly isolated bat lyssaViruses from Eurasia (Aravan, Khujand, and Irkut Viruses). Infection of E. fuscus was moderate (total, 55–75%). There was no evidence of transmission to in-contact cage mates. Incubation periods for Irkut Virus infection were significantly shorter (p < 0.05) than for either Aravan or Khujand Virus infections. In turn, quantification of viral RNA by TaqMan PCR suggests that the dynamics of Irkut Virus infection may differ from those of Aravan/Khujand Virus infection. Although infectious Virus and viral RNA were detected in the brain of every rabid animal, dissemination to non-neuronal tissues was limited. Levels of viral RNA in brain of Aravan/Khujand Virus-infected bats was significantly correlated with the number of other tissues positive by TaqMan PCR (p < 0.05), whereas no such relationship was observed for Irkut Virus infection (where viral RNA was consistently detected in all tissues other than kidney). Infectious Virus was isolated sporadically from salivary glands, and both infectious Virus and viral RNA were obtained from oral swabs. The detection of viral RNA in oral swabs suggests that viral shedding in saliva occurred <5 days before the onset of clinical disease.
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Phylogenetic relationships of Irkut and West Caucasian bat Viruses within the LyssaVirus genus and suggested quantitative criteria based on the N gene sequence for lyssaVirus genotype definition.
Virus research, 2005Co-Authors: Gareth J Hughes, Alexandr D. Botvinkin, Lillian A. OrciariAbstract:The nucleoprotein (N), phosphoprotein (P) and glycoprotein (G) genes of Irkut and West Caucasian bat Viruses (WCBV) were sequenced and compared with those of other lyssaViruses. N gene nucleotide identities provided unequivocal separation of all lyssaVirus genotypes with an identity threshold of 82%. On this basis, Irkut Virus should be considered as a new genotype with particular relatedness to genotypes 4 and 5 (78.0-78.6% identity for N gene nucleotides and 90.4-92.6% for amino acids). Furthermore, genotypes 4-6, together with Aravan, Khujand and Irkut Viruses, present a solid phylogroup of Old World bat lyssaViruses. This relationship is apparent using all three viral genes, and causes overlap between intragenotype and intergenotype identities for the P gene (Aravan, Khujand Viruses and genotype 6) and for the G gene (Aravan, Khujand, genotypes 5 and 6). WCBV is the most divergent of known lyssaViruses with only limited relatedness to genotypes 2 and 3.
Jinxia Zhang - One of the best experts on this subject based on the ideXlab platform.
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analysis of the complete genome of the first Irkut Virus isolate from china comparison across the lyssaVirus genus
Molecular Phylogenetics and Evolution, 2013Co-Authors: Ye Liu, Fei Zhang, Shou Feng Zhang, Hai Lian, Ying Wang, Jinxia ZhangAbstract:The genome of Irkut Virus, isolate IRKV-THChina12, the first non-rabies lyssaVirus from China (of bat origin), has been completely sequenced. In general, coding and non-coding regions of this viral genome are similar to those of other lyssaViruses. However, alignment of the deduced amino acid sequences of the structural proteins of IRKV-THChina12 with those of other lyssaVirus representatives revealed significant variability between viral species. The nucleoprotein and matrix protein were found to be the most conserved, followed by the large protein, glycoprotein and phosphoprotein. Differences in the antigenic sites in glycoprotein may result in only partial protection of the available rabies biologics against Irkut Virus, which is of particular concern for pre- and post-exposure rabies prophylaxis.
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evaluation of rabies biologics against Irkut Virus isolated in china
Journal of Clinical Microbiology, 2013Co-Authors: Ye Liu, Fei Zhang, Shou Feng Zhang, Hai Lian, Ying Wang, Qi Chen, Jinxia ZhangAbstract:An Irkut Virus (IRKV) was recently isolated from a bat in China. The protective ability of rabies biologics available in the Chinese market and experimental biologics against the rabies Virus (RABV) and IRKV were assessed in a hamster model via preexposure prophylaxis (PrEP) and postexposure prophylaxis (PEP) experiments. The results demonstrated that a single dose of rabies vaccine did not induce adequate protection against IRKV infection. However, routine PrEP with three doses of vaccine induced complete protection against IRKV infection. Higher doses of RABV immunoglobulins and alpha interferon were required during PEP to protect hamsters against IRKV versus RABV infection. Experimental recombinant vaccines containing IRKV glycoproteins induced more-reliable protection against IRKV than against RABV infection. Those findings may be explained by limited cross-neutralization of these Viruses (confirmed via in vitro tests) in conjunction with antigenic distances between RABV and IRKV. These results indicate that the development and evaluation of new biologics for PrEP and PEP are required to ensure sufficient protection against IRKV infection in China and other territories where this Virus is present.
