The Experts below are selected from a list of 99651 Experts worldwide ranked by ideXlab platform
Walter Weiss - One of the best experts on this subject based on the ideXlab platform.
-
Detection of polypeptides and amylase Isoenzyme modifications related to malting quality during malting process of barley by two-dimensional electrophoresis and isoelectric focusing with immobilized pH gradients.
Electrophoresis, 1992Co-Authors: Angelika Görg, Wilhelm Postel, Walter WeissAbstract:Two cultivars (“Alexis” and “Lenka”) of contrasting final attenuation values were malted, and the protein and amylase Isoenzyme composition, as well as the change in protein and amylase Isoenzyme composition during malting, was investigated by two-dimensional polyacrylamide gel electrophoresis of total proteins, and isoelectric focusing of amylase Isoenzymes, respectively. Isoelectric focusing demonstrated that significant differences exist between the amylase Isoenzyme patterns of the two cultivars, suggesting a correlation between the presence of certain amylase Isoenzyme bands and final attenuation. This finding was confirmed by analysis of 36 barley cultivars with a wide range of quality. It was shown that all cultivars which are of low or, at best, moderate final attenuation values exhibit the amylase band “B” (isoelectric point ≈ 6.8), whereas those cultivars which are predominantly of high malting grade do not possess this “B” Isoenzyme band, but exhibit the pronounced “A” Isoenzyme band (isoelectric point ≈ 6.5) instead, suggesting that these Isoenzymes (which we suppose to be β-amylases) can be utilized to predict the final attenuation values of unknown barley samples or new lines. However, “final attenuation” is a complex function. Preliminary results of two-dimensional gel electrophoresis indicate that other factors, such as total amount of amylases, or a 19 kDa A hordein-like polypeptide, which was degraded faster in the low malting grade cultivar “Lenka”, may also have a role in determining quality.
-
Detection of polypeptides and amylase Isoenzyme modifications related to malting quality during malting process of barley by two-dimensional electrophoresis and isoelectric focusing with immobilized pH gradients.
Electrophoresis, 1992Co-Authors: Angelika Görg, Wilhelm Postel, Walter WeissAbstract:Two cultivars ("Alexis" and "Lenka") of contrasting final attenuation values were malted, and the protein and amylase Isoenzyme composition, as well as the change in protein and amylase Isoenzyme composition during malting, was investigated by two-dimensional polyacrylamide gel electrophoresis of total proteins, and isoelectric focusing of amylase Isoenzymes, respectively. Isoelectric focusing demonstrated that significant differences exist between the amylase Isoenzyme patterns of the two cultivars, suggesting a correlation between the presence of certain amylase Isoenzyme bands and final attenuation. This finding was confirmed by analysis of 36 barley cultivars with a wide range of quality. It was shown that all cultivars which are of low or, at best, moderate final attenuation values exhibit the amylase band "B" (isoelectric point approximately 6.8), whereas those cultivars which are predominantly of high malting grade do not possess this "B" Isoenzyme band, but exhibit the pronounced "A" Isoenzyme band (isoelectric point approximately 6.5) instead, suggesting that these Isoenzymes (which we suppose to be beta-amylases) can be utilized to predict the final attenuation values of unknown barley samples or new lines. However, "final attenuation" is a complex function. Preliminary results of two-dimensional gel electrophoresis indicate that other factors, such as total amount of amylases, or a 19 kDa A hordein-like polypeptide, which was degraded faster in the low malting grade cultivar "Lenka", may also have a role in determining quality.
Dieter Neumeier - One of the best experts on this subject based on the ideXlab platform.
-
Simultaneous occurrence of the MB Isoenzyme of creatine kinase and macro creatine kinase type 1 in a patient with acute myocardial infarction.
International Journal of Cardiology, 1991Co-Authors: Hannsjörg H.e. Baum, Michael Böhm, Dieter NeumeierAbstract:Abstract When the immunoinhibition method is used for differentiation of Isoenzymes of creatine kinase, the simultaneous occurrance of the MB Isoenzyme and variants of macro creatine kinase may lead to misinterpretation in the diagnosis of acute myocardial infarction. We describe the case of an elderly woman in whom the MB Isoenzyme and the type 1 variant of macro creatine kinase were found simultaneously in her serum after acute myocardial infarction. It proved possible to identify the MB Isoenzyme rapidly using a fast enzymeimmunoassay.
Angelika Görg - One of the best experts on this subject based on the ideXlab platform.
-
Detection of polypeptides and amylase Isoenzyme modifications related to malting quality during malting process of barley by two-dimensional electrophoresis and isoelectric focusing with immobilized pH gradients.
Electrophoresis, 1992Co-Authors: Angelika Görg, Wilhelm Postel, Walter WeissAbstract:Two cultivars (“Alexis” and “Lenka”) of contrasting final attenuation values were malted, and the protein and amylase Isoenzyme composition, as well as the change in protein and amylase Isoenzyme composition during malting, was investigated by two-dimensional polyacrylamide gel electrophoresis of total proteins, and isoelectric focusing of amylase Isoenzymes, respectively. Isoelectric focusing demonstrated that significant differences exist between the amylase Isoenzyme patterns of the two cultivars, suggesting a correlation between the presence of certain amylase Isoenzyme bands and final attenuation. This finding was confirmed by analysis of 36 barley cultivars with a wide range of quality. It was shown that all cultivars which are of low or, at best, moderate final attenuation values exhibit the amylase band “B” (isoelectric point ≈ 6.8), whereas those cultivars which are predominantly of high malting grade do not possess this “B” Isoenzyme band, but exhibit the pronounced “A” Isoenzyme band (isoelectric point ≈ 6.5) instead, suggesting that these Isoenzymes (which we suppose to be β-amylases) can be utilized to predict the final attenuation values of unknown barley samples or new lines. However, “final attenuation” is a complex function. Preliminary results of two-dimensional gel electrophoresis indicate that other factors, such as total amount of amylases, or a 19 kDa A hordein-like polypeptide, which was degraded faster in the low malting grade cultivar “Lenka”, may also have a role in determining quality.
-
Detection of polypeptides and amylase Isoenzyme modifications related to malting quality during malting process of barley by two-dimensional electrophoresis and isoelectric focusing with immobilized pH gradients.
Electrophoresis, 1992Co-Authors: Angelika Görg, Wilhelm Postel, Walter WeissAbstract:Two cultivars ("Alexis" and "Lenka") of contrasting final attenuation values were malted, and the protein and amylase Isoenzyme composition, as well as the change in protein and amylase Isoenzyme composition during malting, was investigated by two-dimensional polyacrylamide gel electrophoresis of total proteins, and isoelectric focusing of amylase Isoenzymes, respectively. Isoelectric focusing demonstrated that significant differences exist between the amylase Isoenzyme patterns of the two cultivars, suggesting a correlation between the presence of certain amylase Isoenzyme bands and final attenuation. This finding was confirmed by analysis of 36 barley cultivars with a wide range of quality. It was shown that all cultivars which are of low or, at best, moderate final attenuation values exhibit the amylase band "B" (isoelectric point approximately 6.8), whereas those cultivars which are predominantly of high malting grade do not possess this "B" Isoenzyme band, but exhibit the pronounced "A" Isoenzyme band (isoelectric point approximately 6.5) instead, suggesting that these Isoenzymes (which we suppose to be beta-amylases) can be utilized to predict the final attenuation values of unknown barley samples or new lines. However, "final attenuation" is a complex function. Preliminary results of two-dimensional gel electrophoresis indicate that other factors, such as total amount of amylases, or a 19 kDa A hordein-like polypeptide, which was degraded faster in the low malting grade cultivar "Lenka", may also have a role in determining quality.
Richard J. Smith - One of the best experts on this subject based on the ideXlab platform.
-
Aspartate kinase regulation in maize: Evidence for co-purification of threonine-sensitive aspartate kinase and homoserine dehydrogenase.
Phytochemistry, 1992Co-Authors: Ricardo Antunes Azevedo, Richard J. SmithAbstract:The threonine-sensitive and resistant forms of homoserine dehydrogenase activities were assayed in all fractions obtained during the purification of three aspartate kinase Isoenzymes (threonine, lysine and lysine plus S-adenosylmethionine-sensitive) from maize cultures. The homoserine dehydrogenase Isoenzyme resistant to inhibition by threonine, has a molecular weight of 70 000 and could be easily separated from the three aspartate kinase Isoenzymes by ion-exchange chromatography and gel filtration; similarly the two lysine-sensitive forms of aspartate kinase could be separated from both forms of homoserine dehydrogenase. The homoserine dehydrogenase sensitive to threonine inhibition has a molecular weight of 180 000 and co-purified with the threonine-sensitive aspartate kinase Isoenzyme. The hypothesis of the presence of a bifunctional protein containing activities of aspartate kinase-homoserine dehydrogenase is discussed.
Johann Hofmann - One of the best experts on this subject based on the ideXlab platform.
-
the potential for Isoenzyme selective modulation of protein kinase c
The FASEB Journal, 1997Co-Authors: Johann HofmannAbstract:Protein kinase C (PKC) is a phospholipid-dependent serine/threonine kinase family consisting of at least 11 closely related Isoenzymes. The different PKC Isoenzymes play important roles in signal transduction pathways. The exact significance of each Isoenzyme is not known at present; therefore, the elucidation of the roles of the various PKC Isoenzymes is important. To explain the function of distinct PKC Isoenzymes, the availability of Isoenzyme-specific inhibibitors or activators would be an advantage. PKC inhibitors have been known for some time, but these compounds are not Isoenzyme-specific and also inhibit other kinases. Recently, an inhibitor selective for PKC alpha and another one selective for PKCbetaI and betaII were described. Both compounds compete with the ATP binding sites that exhibit high homologies among the different PKC Isoenzymes. Among others, the phosporyl transfer region, the pseudosubstrate domain, the phorbolester binding sequences, and the phosphorylation sites may also be target...
