Isogenic Lines

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J. M. Bonman - One of the best experts on this subject based on the ideXlab platform.

  • Allelism of blast resistance genes in near-Isogenic Lines of rice
    Phytopathology, 1994
    Co-Authors: Tsuyoshi Inukai, J. M. Bonman, Rebecca J. Nelson, Robert S. Zeigler, S. Sarkarung, David J. Mackill, I. Takamure, Toshiro Kinoshita
    Abstract:

    A set of near-Isogenic Lines (NILs) for rice blast resistance was previously developed in the genetic background of indica rice cultivar CO39. The 22 CO39 NILs carry five blast resistance genes. In this report, allelism between these resistance genes in the CO39 NILs and Kiyosawa's Differential series of cultivars was analyzed. Pi-l(t), carried by the NILs in group I, was closely linked to Pi-k on chromosome 11, with a recombination value of 6.1 ± 1.7% (standard error [SE]). Pi-2(t), carried by the NILs in group II, was allelic or closely linked to Pi-z on chromosome 6. Pi-3(t), present in the group V NIL, was closely linked to Pi-i, with a recombination value of 7.0 ± 2.6% SE

  • inheritance of blast resistance in near Isogenic Lines of rice
    Phytopathology, 1992
    Co-Authors: David J. Mackill, J. M. Bonman
    Abstract:

    Resistance to blast disease is an important objective of most rice breeding programs. Genetic studies of resistance have been complicated by variability of the pathogen and lack of rice genotypes with single resistance genes. Near-Isogenic Lines (NILs) with single blast resistance genes were developed by backcrossing four donor cultivars to the recurrent parent CO39. Five pathogen isolates were used to screen the populations during backcrossing. The 22 NILs were classified into six groups by their reaction to a diverse set of blast isolates (...)

David J. Mackill - One of the best experts on this subject based on the ideXlab platform.

  • Allelism of blast resistance genes in near-Isogenic Lines of rice
    Phytopathology, 1994
    Co-Authors: Tsuyoshi Inukai, J. M. Bonman, Rebecca J. Nelson, Robert S. Zeigler, S. Sarkarung, David J. Mackill, I. Takamure, Toshiro Kinoshita
    Abstract:

    A set of near-Isogenic Lines (NILs) for rice blast resistance was previously developed in the genetic background of indica rice cultivar CO39. The 22 CO39 NILs carry five blast resistance genes. In this report, allelism between these resistance genes in the CO39 NILs and Kiyosawa's Differential series of cultivars was analyzed. Pi-l(t), carried by the NILs in group I, was closely linked to Pi-k on chromosome 11, with a recombination value of 6.1 ± 1.7% (standard error [SE]). Pi-2(t), carried by the NILs in group II, was allelic or closely linked to Pi-z on chromosome 6. Pi-3(t), present in the group V NIL, was closely linked to Pi-i, with a recombination value of 7.0 ± 2.6% SE

  • inheritance of blast resistance in near Isogenic Lines of rice
    Phytopathology, 1992
    Co-Authors: David J. Mackill, J. M. Bonman
    Abstract:

    Resistance to blast disease is an important objective of most rice breeding programs. Genetic studies of resistance have been complicated by variability of the pathogen and lack of rice genotypes with single resistance genes. Near-Isogenic Lines (NILs) with single blast resistance genes were developed by backcrossing four donor cultivars to the recurrent parent CO39. Five pathogen isolates were used to screen the populations during backcrossing. The 22 NILs were classified into six groups by their reaction to a diverse set of blast isolates (...)

S. N. Sibikeev - One of the best experts on this subject based on the ideXlab platform.

Steven D. Tanksley - One of the best experts on this subject based on the ideXlab platform.

  • Rapid identification of markers linked to a Pseudomonas resistance gene in tomato by using random primers and near-Isogenic Lines.
    Proceedings of the National Academy of Sciences of the United States of America, 1991
    Co-Authors: Gregory B. Martin, John G. K. Williams, Steven D. Tanksley
    Abstract:

    Abstract An approach to isolating DNA sequences that are linked to important plant genes is described. The strategy is based upon a recent modification of the polymerase chain reaction in which synthetic primers are used to amplify random sequences from genomic DNA. This technique, used in conjunction with near-Isogenic Lines (which differ only by the presence or absence of the target gene and a small region of surrounding DNA), leads to the rapid identification of sequences linked to the gene of interest. The feasibility of this method has been demonstrated by analyzing a pair of tomato near-Isogenic Lines that differ for a region on chromosome 5 that contains a gene (Pto) conferring resistance to Pseudomonas syringae pv. tomato. One hundred forty-four random primers were screened on these Lines, and seven amplified products were identified that were present in one but not the other line. Of four products that were further investigated, three were confirmed by segregation analysis to be tightly linked to the Pto gene. Linked sequences identified by this method are useful for detecting the presence of the target gene in plant populations (e.g., in plant breeding) and, if very tightly linked, as starting points for a chromosome walk to isolate the gene. Since near-Isogenic Lines are a typical product of plant breeding and classical genetic studies, this method is applicable to a wide variety of species.

Gary J. Muehlbauer - One of the best experts on this subject based on the ideXlab platform.

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