The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Ugo Leuzzi - One of the best experts on this subject based on the ideXlab platform.
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flavonol glucoside profile of southern italian red onion allium cepa l
Journal of Agricultural and Food Chemistry, 2005Co-Authors: Paola Bonaccorsi, Corrado Caristi, Claudia Gargiulli, Ugo LeuzziAbstract:High-performance liquid chromatography−diode array detector (HPLC−DAD) coupled with electron spray mass spectrometry (ESI−MS−MS) was used to determine the flavonol profile in southern Italian red onions (Allium cepa L.). This on-line technique allowed the identification of seven flavonols in southern Italian red onion, quercetin 4‘-glucoside and quercetin 3,4‘-diglucoside being the most abundant components. Five minor flavonols have been recognized, offering a characteristic profile of such compounds in red onions under study. Quercetin 3-glucoside, quercetin 7,4‘-diglucoside, quercetin 3,7,4‘-triglucoside, and Isorhamnetin 4‘-glucoside have been previously reported as minor flavonoid components in Allium cepa, while Isorhamnetin 3,4‘-diglucoside was previously found in Allium ascalonicum. Traces of Isorhamnetin 3-glucoside and free quercetin were also detected. Keywords: Allium cepa; flavonols; quercetin; Isorhamnetin; HPLC−DAD; ESI−MS−MS
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flavonol glucoside profile of southern italian red onion allium cepa l
Journal of Agricultural and Food Chemistry, 2005Co-Authors: Paola Bonaccorsi, Corrado Caristi, Claudia Gargiulli, Ugo LeuzziAbstract:High-performance liquid chromatography-diode array detector (HPLC-DAD) coupled with electron spray mass spectrometry (ESI-MS-MS) was used to determine the flavonol profile in southern Italian red onions (Allium cepa L.). This on-line technique allowed the identification of seven flavonols in southern Italian red onion, quercetin 4'-glucoside and quercetin 3,4'-diglucoside being the most abundant components. Five minor flavonols have been recognized, offering a characteristic profile of such compounds in red onions under study. Quercetin 3-glucoside, quercetin 7,4'-diglucoside, quercetin 3,7,4'-triglucoside, and Isorhamnetin 4'-glucoside have been previously reported as minor flavonoid components in Allium cepa, while Isorhamnetin 3,4'-diglucoside was previously found in Allium ascalonicum. Traces of Isorhamnetin 3-glucoside and free quercetin were also detected.
Marilena Antunesricardo - One of the best experts on this subject based on the ideXlab platform.
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enzyme assisted supercritical fluid extraction of antioxidant Isorhamnetin conjugates from opuntia ficus indica l mill
Journal of Supercritical Fluids, 2020Co-Authors: Marilena Antunesricardo, Janet A Gutierrezuribe, Tomas Garciacayuela, J A Mendiola, Elena Ibanez, Pilar M Cano, Daniel GuajardofloresAbstract:Abstract A one-pot enzymatic assisted supercritical carbon dioxide (SC-CO2) extraction was used to obtain Isorhamnetin conjugates from Opuntia ficus-indica (L.) Mill (nopal). Box-Benhken design, discriminate analysis and principal component analysis were used to studt the influence of pressure, temperature, aqueous ethanol solution and time on the total extraction yield and the Isorhamnetin conjugates recovery using the enzymes Rapidase Maxi Fruit (Rapidase) and Viscozyme. Isorhamnetin tri- and diglycoside were found in the extracts along with other phenolic compounds such as 3-O-methyl quercetin, 3-O-methyl kaempferol and luteolin. The Isorhamnetin and phenolic profile patterns were dependent on the conditions used. Cellular antioxidant activity was positively correlated to Isorhamnetin-3-O-glucosyl-rhamnoside and luteolin content. The best results were obtained by using Rapidase, a currently used enzyme for the winemaking industry. The use of enzymes under SC-CO2 conditions affected the release of Isorhamnetin conjugate profiles which induced remarkable differences in the cellular antioxidant activity.
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supercritical co2 enzyme hydrolysis as a pretreatment for the release of Isorhamnetin conjugates from opuntia ficus indica l mill
Journal of Supercritical Fluids, 2017Co-Authors: Marilena Antunesricardo, Janet A Gutierrezuribe, Tomas Garciacayuela, J A Mendiola, Elena Ibanez, Pilar M Cano, Daniel GuajardofloresAbstract:Abstract Isorhamnetin conjugates from Opuntia ficus-indica (L.) Mill (nopal) were obtained using enzymatic hydrolysis pretreatment under supercritical carbon dioxide (SC-CO2) conditions. Factorial designs and discriminate analysis were used to evaluate the influence of pressure, temperature, pH, time and aqueous ethanol solution on the total extraction yield and the Isorhamnetin recovery using the enzymes Rapidase Maxi Fruit (RMF) and Viscozyme. Isorhamnetin triglycosides [Isorhamnetin-3-O-glucosyl-rhamnosyl-rhamnoside (IG1) and Isorhamnetin-3-O-glucosyl-rhamnosyl-pentoside (IG2)] and a diglycoside [Isorhamnetin-3-O-glucosyl-rhmanoside (IG5)] were found in the extracts. The Isorhamnetin profile pattern was dependent on the enzyme and process conditions used. The extract containing higher amounts of IG5 was the most potent inhibitor of nitric oxide reducing its production by 71.6 ± 4.8%. Selective extraction of IG5 was achieved using RMF enzyme and optimal conditions determined by a response surface methodology. The use of enzyme under SC-CO2 conditions affected the release of Isorhamnetin conjugate profiles which induced remarkable differences in the anti-inflammatory activity.
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bioaccessibility intestinal permeability and plasma stability of Isorhamnetin glycosides from opuntia ficus indica l
International Journal of Molecular Sciences, 2017Co-Authors: Marilena Antunesricardo, Cesar Rodriguezrodriguez, Janet A Gutierrezuribe, Eduardo Cepedacanedo, Sergio O SernasaldivarAbstract:Isorhamnetin glycosides are representative compounds of Opuntia ficus-indica that possess different biological activities. There is slight information about the changes in bioaccessibility induced by the glycosylation pattern of flavonoids, particularly for Isorhamnetin. In this study, the bioaccessibility and permeability of Isorhamnetin glycosides extracted from O. ficus-indica were contrasted with an Isorhamnetin standard. Also, the plasma stability of these Isorhamnetin glycosides after intravenous administration in rats was evaluated. Recoveries of Isorhamnetin after oral and gastric digestion were lower than that observed for its glycosides. After intestinal digestion, Isorhamnetin glycosides recoveries were reduced to less than 81.0%. The apparent permeability coefficient from apical (AP) to basolateral (BL) direction (Papp(AP-BL)) of Isorhamnetin was 2.6 to 4.6-fold higher than those obtained for its glycosides. Isorhamnetin diglycosides showed higher Papp(AP-BL) values than triglycosides. Sugar substituents affected the Papp(AP-BL) of the triglycosides. Isorhamnetin glycosides were better retained in the circulatory system than the aglycone. After intravenous dose of the Isorhamnetin standard, the elimination half-life was 0.64 h but increased to 1.08 h when the O. ficus-indica extract was administered. These results suggest that Isorhamnetin glycosides naturally found in O. ficus-indica could be a controlled delivery system to maintain a constant plasmatic concentration of this important flavonoid to exert its biological effects in vivo.
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extraction of Isorhamnetin conjugates from opuntia ficus indica l mill using supercritical fluids
Journal of Supercritical Fluids, 2017Co-Authors: Marilena Antunesricardo, Janet A Gutierrezuribe, Daniel GuajardofloresAbstract:Abstract Flavonoids from Opuntia ficus-indica (L.) Mill were extracted using supercritical carbon dioxide (SC-CO2) technique. A central composite design was used to evaluate the influence of pressure, temperature and flow rate on the total extract yield and the Isorhamnetin recovery. Isorhamnetin-3-O-glucosyl-rhamnosyl-rhamnoside, Isorhamnetin-3-O-glucosyl-rhamnosyl-pentoside, Isorhamnetin-3-O-glucosyl-rhamnoside, Isorhamnetin-3-O-glucosyl-pentoside were the most abundant flavonoids in O. ficus-indica supercritical extracts. Pressure had a significant effect on the extraction yield and Isorhamnetin recovery, particularly a quadratic response was observed for extraction yield. A similar flavonoids profile than the observed for the methanolic extract or after alkaline hydrolysis was obtained with the following conditions: 50°C, 300 bar and a CO2 flow rate of 80 g/min. SC-CO2 technique allowed the preferential extraction of 3-O-methyl-quercetin at 400 bar, 35 °C, and a flow rate of 100 g/min. This report shows the potential uses of SC-CO2 as an alternative solvent free methodology to extract high value ingredients from natural sources.
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in vivo anti inflammatory effects of Isorhamnetin glycosides isolated from opuntia ficus indica l mill cladodes
Industrial Crops and Products, 2015Co-Authors: Marilena Antunesricardo, Janet A Gutierrezuribe, Mario Moises Alvarez, Felipe Lopezpacheco, Sergio O SernasaldivarAbstract:Abstract Opuntia ficus-indica (L.) Mill [OFI] has been traditionally used to reduce inflammation. This plant possesses a varied composition of phytochemicals such as flavonoids. The aim of this investigation was to study the anti-inflammatory effects of an OFI extract and two isolated Isorhamnetin glycosides using an air-pouch inflammation model. Isorhamnetin glycosides were isolated by semi-preparative chromatography and identified with LC/MSD TOF. Male Wistar rats were divided into five groups (n = 6) receiving Isorhamnetin-3-O-glucosyl-rhamnosyl-rhamnoside (IGRR), Isorhamnetin-3-O-glucosyl-rhamnoside (IGR), OFI extract, indomethacin or vehicle intraperitoneally. IGR showed the best potential to decrease cell infiltration, mainly neutrophils (51.8 ± 10.9%). IGRR and OFI extract inhibited the nitric oxide production (IGRR: 81.4%; OFI extract: 77.2%) and cyclooxygenase-2 activity (IGRR: 76.3%; OFI extract: 77.7%). OFI extract showed the highest inhibition of tumor necrosis factor (TNF)-α (85.2%) and interleukin (IL)-6 (53.0%). Results showed that OFI extract and Isorhamnetin glycosides possess anti-inflammatory potential. IGRR showed better effect than IGR suggesting that the glycosylation profile affects the bioactivity.
Paola Bonaccorsi - One of the best experts on this subject based on the ideXlab platform.
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flavonol glucoside profile of southern italian red onion allium cepa l
Journal of Agricultural and Food Chemistry, 2005Co-Authors: Paola Bonaccorsi, Corrado Caristi, Claudia Gargiulli, Ugo LeuzziAbstract:High-performance liquid chromatography−diode array detector (HPLC−DAD) coupled with electron spray mass spectrometry (ESI−MS−MS) was used to determine the flavonol profile in southern Italian red onions (Allium cepa L.). This on-line technique allowed the identification of seven flavonols in southern Italian red onion, quercetin 4‘-glucoside and quercetin 3,4‘-diglucoside being the most abundant components. Five minor flavonols have been recognized, offering a characteristic profile of such compounds in red onions under study. Quercetin 3-glucoside, quercetin 7,4‘-diglucoside, quercetin 3,7,4‘-triglucoside, and Isorhamnetin 4‘-glucoside have been previously reported as minor flavonoid components in Allium cepa, while Isorhamnetin 3,4‘-diglucoside was previously found in Allium ascalonicum. Traces of Isorhamnetin 3-glucoside and free quercetin were also detected. Keywords: Allium cepa; flavonols; quercetin; Isorhamnetin; HPLC−DAD; ESI−MS−MS
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flavonol glucoside profile of southern italian red onion allium cepa l
Journal of Agricultural and Food Chemistry, 2005Co-Authors: Paola Bonaccorsi, Corrado Caristi, Claudia Gargiulli, Ugo LeuzziAbstract:High-performance liquid chromatography-diode array detector (HPLC-DAD) coupled with electron spray mass spectrometry (ESI-MS-MS) was used to determine the flavonol profile in southern Italian red onions (Allium cepa L.). This on-line technique allowed the identification of seven flavonols in southern Italian red onion, quercetin 4'-glucoside and quercetin 3,4'-diglucoside being the most abundant components. Five minor flavonols have been recognized, offering a characteristic profile of such compounds in red onions under study. Quercetin 3-glucoside, quercetin 7,4'-diglucoside, quercetin 3,7,4'-triglucoside, and Isorhamnetin 4'-glucoside have been previously reported as minor flavonoid components in Allium cepa, while Isorhamnetin 3,4'-diglucoside was previously found in Allium ascalonicum. Traces of Isorhamnetin 3-glucoside and free quercetin were also detected.
Janet A Gutierrezuribe - One of the best experts on this subject based on the ideXlab platform.
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enzyme assisted supercritical fluid extraction of antioxidant Isorhamnetin conjugates from opuntia ficus indica l mill
Journal of Supercritical Fluids, 2020Co-Authors: Marilena Antunesricardo, Janet A Gutierrezuribe, Tomas Garciacayuela, J A Mendiola, Elena Ibanez, Pilar M Cano, Daniel GuajardofloresAbstract:Abstract A one-pot enzymatic assisted supercritical carbon dioxide (SC-CO2) extraction was used to obtain Isorhamnetin conjugates from Opuntia ficus-indica (L.) Mill (nopal). Box-Benhken design, discriminate analysis and principal component analysis were used to studt the influence of pressure, temperature, aqueous ethanol solution and time on the total extraction yield and the Isorhamnetin conjugates recovery using the enzymes Rapidase Maxi Fruit (Rapidase) and Viscozyme. Isorhamnetin tri- and diglycoside were found in the extracts along with other phenolic compounds such as 3-O-methyl quercetin, 3-O-methyl kaempferol and luteolin. The Isorhamnetin and phenolic profile patterns were dependent on the conditions used. Cellular antioxidant activity was positively correlated to Isorhamnetin-3-O-glucosyl-rhamnoside and luteolin content. The best results were obtained by using Rapidase, a currently used enzyme for the winemaking industry. The use of enzymes under SC-CO2 conditions affected the release of Isorhamnetin conjugate profiles which induced remarkable differences in the cellular antioxidant activity.
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supercritical co2 enzyme hydrolysis as a pretreatment for the release of Isorhamnetin conjugates from opuntia ficus indica l mill
Journal of Supercritical Fluids, 2017Co-Authors: Marilena Antunesricardo, Janet A Gutierrezuribe, Tomas Garciacayuela, J A Mendiola, Elena Ibanez, Pilar M Cano, Daniel GuajardofloresAbstract:Abstract Isorhamnetin conjugates from Opuntia ficus-indica (L.) Mill (nopal) were obtained using enzymatic hydrolysis pretreatment under supercritical carbon dioxide (SC-CO2) conditions. Factorial designs and discriminate analysis were used to evaluate the influence of pressure, temperature, pH, time and aqueous ethanol solution on the total extraction yield and the Isorhamnetin recovery using the enzymes Rapidase Maxi Fruit (RMF) and Viscozyme. Isorhamnetin triglycosides [Isorhamnetin-3-O-glucosyl-rhamnosyl-rhamnoside (IG1) and Isorhamnetin-3-O-glucosyl-rhamnosyl-pentoside (IG2)] and a diglycoside [Isorhamnetin-3-O-glucosyl-rhmanoside (IG5)] were found in the extracts. The Isorhamnetin profile pattern was dependent on the enzyme and process conditions used. The extract containing higher amounts of IG5 was the most potent inhibitor of nitric oxide reducing its production by 71.6 ± 4.8%. Selective extraction of IG5 was achieved using RMF enzyme and optimal conditions determined by a response surface methodology. The use of enzyme under SC-CO2 conditions affected the release of Isorhamnetin conjugate profiles which induced remarkable differences in the anti-inflammatory activity.
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bioaccessibility intestinal permeability and plasma stability of Isorhamnetin glycosides from opuntia ficus indica l
International Journal of Molecular Sciences, 2017Co-Authors: Marilena Antunesricardo, Cesar Rodriguezrodriguez, Janet A Gutierrezuribe, Eduardo Cepedacanedo, Sergio O SernasaldivarAbstract:Isorhamnetin glycosides are representative compounds of Opuntia ficus-indica that possess different biological activities. There is slight information about the changes in bioaccessibility induced by the glycosylation pattern of flavonoids, particularly for Isorhamnetin. In this study, the bioaccessibility and permeability of Isorhamnetin glycosides extracted from O. ficus-indica were contrasted with an Isorhamnetin standard. Also, the plasma stability of these Isorhamnetin glycosides after intravenous administration in rats was evaluated. Recoveries of Isorhamnetin after oral and gastric digestion were lower than that observed for its glycosides. After intestinal digestion, Isorhamnetin glycosides recoveries were reduced to less than 81.0%. The apparent permeability coefficient from apical (AP) to basolateral (BL) direction (Papp(AP-BL)) of Isorhamnetin was 2.6 to 4.6-fold higher than those obtained for its glycosides. Isorhamnetin diglycosides showed higher Papp(AP-BL) values than triglycosides. Sugar substituents affected the Papp(AP-BL) of the triglycosides. Isorhamnetin glycosides were better retained in the circulatory system than the aglycone. After intravenous dose of the Isorhamnetin standard, the elimination half-life was 0.64 h but increased to 1.08 h when the O. ficus-indica extract was administered. These results suggest that Isorhamnetin glycosides naturally found in O. ficus-indica could be a controlled delivery system to maintain a constant plasmatic concentration of this important flavonoid to exert its biological effects in vivo.
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extraction of Isorhamnetin conjugates from opuntia ficus indica l mill using supercritical fluids
Journal of Supercritical Fluids, 2017Co-Authors: Marilena Antunesricardo, Janet A Gutierrezuribe, Daniel GuajardofloresAbstract:Abstract Flavonoids from Opuntia ficus-indica (L.) Mill were extracted using supercritical carbon dioxide (SC-CO2) technique. A central composite design was used to evaluate the influence of pressure, temperature and flow rate on the total extract yield and the Isorhamnetin recovery. Isorhamnetin-3-O-glucosyl-rhamnosyl-rhamnoside, Isorhamnetin-3-O-glucosyl-rhamnosyl-pentoside, Isorhamnetin-3-O-glucosyl-rhamnoside, Isorhamnetin-3-O-glucosyl-pentoside were the most abundant flavonoids in O. ficus-indica supercritical extracts. Pressure had a significant effect on the extraction yield and Isorhamnetin recovery, particularly a quadratic response was observed for extraction yield. A similar flavonoids profile than the observed for the methanolic extract or after alkaline hydrolysis was obtained with the following conditions: 50°C, 300 bar and a CO2 flow rate of 80 g/min. SC-CO2 technique allowed the preferential extraction of 3-O-methyl-quercetin at 400 bar, 35 °C, and a flow rate of 100 g/min. This report shows the potential uses of SC-CO2 as an alternative solvent free methodology to extract high value ingredients from natural sources.
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in vivo anti inflammatory effects of Isorhamnetin glycosides isolated from opuntia ficus indica l mill cladodes
Industrial Crops and Products, 2015Co-Authors: Marilena Antunesricardo, Janet A Gutierrezuribe, Mario Moises Alvarez, Felipe Lopezpacheco, Sergio O SernasaldivarAbstract:Abstract Opuntia ficus-indica (L.) Mill [OFI] has been traditionally used to reduce inflammation. This plant possesses a varied composition of phytochemicals such as flavonoids. The aim of this investigation was to study the anti-inflammatory effects of an OFI extract and two isolated Isorhamnetin glycosides using an air-pouch inflammation model. Isorhamnetin glycosides were isolated by semi-preparative chromatography and identified with LC/MSD TOF. Male Wistar rats were divided into five groups (n = 6) receiving Isorhamnetin-3-O-glucosyl-rhamnosyl-rhamnoside (IGRR), Isorhamnetin-3-O-glucosyl-rhamnoside (IGR), OFI extract, indomethacin or vehicle intraperitoneally. IGR showed the best potential to decrease cell infiltration, mainly neutrophils (51.8 ± 10.9%). IGRR and OFI extract inhibited the nitric oxide production (IGRR: 81.4%; OFI extract: 77.2%) and cyclooxygenase-2 activity (IGRR: 76.3%; OFI extract: 77.7%). OFI extract showed the highest inhibition of tumor necrosis factor (TNF)-α (85.2%) and interleukin (IL)-6 (53.0%). Results showed that OFI extract and Isorhamnetin glycosides possess anti-inflammatory potential. IGRR showed better effect than IGR suggesting that the glycosylation profile affects the bioactivity.
Daniel Guajardoflores - One of the best experts on this subject based on the ideXlab platform.
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enzyme assisted supercritical fluid extraction of antioxidant Isorhamnetin conjugates from opuntia ficus indica l mill
Journal of Supercritical Fluids, 2020Co-Authors: Marilena Antunesricardo, Janet A Gutierrezuribe, Tomas Garciacayuela, J A Mendiola, Elena Ibanez, Pilar M Cano, Daniel GuajardofloresAbstract:Abstract A one-pot enzymatic assisted supercritical carbon dioxide (SC-CO2) extraction was used to obtain Isorhamnetin conjugates from Opuntia ficus-indica (L.) Mill (nopal). Box-Benhken design, discriminate analysis and principal component analysis were used to studt the influence of pressure, temperature, aqueous ethanol solution and time on the total extraction yield and the Isorhamnetin conjugates recovery using the enzymes Rapidase Maxi Fruit (Rapidase) and Viscozyme. Isorhamnetin tri- and diglycoside were found in the extracts along with other phenolic compounds such as 3-O-methyl quercetin, 3-O-methyl kaempferol and luteolin. The Isorhamnetin and phenolic profile patterns were dependent on the conditions used. Cellular antioxidant activity was positively correlated to Isorhamnetin-3-O-glucosyl-rhamnoside and luteolin content. The best results were obtained by using Rapidase, a currently used enzyme for the winemaking industry. The use of enzymes under SC-CO2 conditions affected the release of Isorhamnetin conjugate profiles which induced remarkable differences in the cellular antioxidant activity.
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supercritical co2 enzyme hydrolysis as a pretreatment for the release of Isorhamnetin conjugates from opuntia ficus indica l mill
Journal of Supercritical Fluids, 2017Co-Authors: Marilena Antunesricardo, Janet A Gutierrezuribe, Tomas Garciacayuela, J A Mendiola, Elena Ibanez, Pilar M Cano, Daniel GuajardofloresAbstract:Abstract Isorhamnetin conjugates from Opuntia ficus-indica (L.) Mill (nopal) were obtained using enzymatic hydrolysis pretreatment under supercritical carbon dioxide (SC-CO2) conditions. Factorial designs and discriminate analysis were used to evaluate the influence of pressure, temperature, pH, time and aqueous ethanol solution on the total extraction yield and the Isorhamnetin recovery using the enzymes Rapidase Maxi Fruit (RMF) and Viscozyme. Isorhamnetin triglycosides [Isorhamnetin-3-O-glucosyl-rhamnosyl-rhamnoside (IG1) and Isorhamnetin-3-O-glucosyl-rhamnosyl-pentoside (IG2)] and a diglycoside [Isorhamnetin-3-O-glucosyl-rhmanoside (IG5)] were found in the extracts. The Isorhamnetin profile pattern was dependent on the enzyme and process conditions used. The extract containing higher amounts of IG5 was the most potent inhibitor of nitric oxide reducing its production by 71.6 ± 4.8%. Selective extraction of IG5 was achieved using RMF enzyme and optimal conditions determined by a response surface methodology. The use of enzyme under SC-CO2 conditions affected the release of Isorhamnetin conjugate profiles which induced remarkable differences in the anti-inflammatory activity.
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extraction of Isorhamnetin conjugates from opuntia ficus indica l mill using supercritical fluids
Journal of Supercritical Fluids, 2017Co-Authors: Marilena Antunesricardo, Janet A Gutierrezuribe, Daniel GuajardofloresAbstract:Abstract Flavonoids from Opuntia ficus-indica (L.) Mill were extracted using supercritical carbon dioxide (SC-CO2) technique. A central composite design was used to evaluate the influence of pressure, temperature and flow rate on the total extract yield and the Isorhamnetin recovery. Isorhamnetin-3-O-glucosyl-rhamnosyl-rhamnoside, Isorhamnetin-3-O-glucosyl-rhamnosyl-pentoside, Isorhamnetin-3-O-glucosyl-rhamnoside, Isorhamnetin-3-O-glucosyl-pentoside were the most abundant flavonoids in O. ficus-indica supercritical extracts. Pressure had a significant effect on the extraction yield and Isorhamnetin recovery, particularly a quadratic response was observed for extraction yield. A similar flavonoids profile than the observed for the methanolic extract or after alkaline hydrolysis was obtained with the following conditions: 50°C, 300 bar and a CO2 flow rate of 80 g/min. SC-CO2 technique allowed the preferential extraction of 3-O-methyl-quercetin at 400 bar, 35 °C, and a flow rate of 100 g/min. This report shows the potential uses of SC-CO2 as an alternative solvent free methodology to extract high value ingredients from natural sources.