The Experts below are selected from a list of 123 Experts worldwide ranked by ideXlab platform
Tomo Inomata - One of the best experts on this subject based on the ideXlab platform.
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fertility of spermatozoa cryopreserved with 2 acetamide or glycerol through artificial insemination in the Japanese White Rabbit
Experimental Animals, 2007Co-Authors: Yasushi Okuda, Yasunari Seita, Shin Hisamatsu, Shigenori Sonoki, Masao Shino, Toshio Masaoka, Tomo Inomata, Shinichi Kamijo, Naomi KashiwazakiAbstract:The Rabbit is considered to be a valuable laboratory animal. We compared 2% acetamide and glycerol as cryoprotectants in egg-yolk diluent for ejaculated Japanese White Rabbit spermatozoa to improve sperm cryopreservation methods. Fertility through artificial insemination, forward progressive motility and plasma membrane integrity of the post-thaw spermatozoa were examined. The rates of forward progressive motility and plasma membrane integrity of the spermatozoa frozen with acetamide (27.1 ± 8.3% and 24.5 ± 6.5%) were significantly (P<0.05) higher than those of the spermatozoa frozen with glycerol (16.3 ± 10.9% and 14.3 ± 7.6%). Though there was no significant difference in the kindling rates, the litter size of females inseminated with spermatozoa frozen with acetamide (6.0 ± 1.1) were significantly (P<0.05) higher than those of spermatozoa frozen with glycerol (3.0 ± 0.4). The results indicate that 2% acetamide has a higher cryoprotective effect than 2% glycerol for sperm cryopreservation in the Japanese White Rabbit.
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Fertility of spermatozoa cryopreserved with 2% acetamide or glycerol through artificial insemination in the Japanese White Rabbit.
Experimental animals, 2007Co-Authors: Yasushi Okuda, Yasunari Seita, Shin Hisamatsu, Shigenori Sonoki, Masao Shino, Toshio Masaoka, Tomo Inomata, Shinichi Kamijo, Naomi KashiwazakiAbstract:The Rabbit is considered to be a valuable laboratory animal. We compared 2% acetamide and glycerol as cryoprotectants in egg-yolk diluent for ejaculated Japanese White Rabbit spermatozoa to improve sperm cryopreservation methods. Fertility through artificial insemination, forward progressive motility and plasma membrane integrity of the post-thaw spermatozoa were examined. The rates of forward progressive motility and plasma membrane integrity of the spermatozoa frozen with acetamide (27.1 ± 8.3% and 24.5 ± 6.5%) were significantly (P
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comparison of glycerol lactamide acetamide and dimethylsulfoxide as cryoprotectants of Japanese White Rabbit spermatozoa
Journal of Reproduction and Development, 2006Co-Authors: Naomi Kashiwazaki, Yasushi Okuda, Yasunari Seita, Shin Hisamatsu, Shigenori Sonoki, Masao Shino, Toshio Masaoka, Tomo InomataAbstract:The Rabbit is considered to be a valuable laboratory animal. We compared glycerol, lactamide, acetamide, and dimethylsulfoxide (DMSO) as cryoprotectants in egg-yolk diluent of ejaculated Japanese White Rabbit spermatozoa for improvement of sperm cryopreservation methods. Rabbit semen was frozen with 1.0 M glycerol, lactamide, acetamide, or DMSO in plastic straws. Forward progressive motility and plasma membrane integrity of the post-thaw spermatozoa were examined. The rate of forward progressive motile spermatozoa in lactamide (37.8 +/- 3.0%) was significantly (P<0.05) higher than in glycerol (17.0 +/- 3.3%). In addition, the rates of sperm plasma membrane integrity in lactamide and acetamide (35.9 +/- 3.3% and 30.2 +/- 3.0%, respectively) were significantly (P<0.05) higher than in glycerol (17.0 +/- 2.6%). The results indicate that 1.0 M lactamide and acetamide have higher cryoprotective effects than 1.0 M glycerol for cryopreservation of Japanese White Rabbit spermatozoa.
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Comparison of Glycerol, Lactamide, Acetamide and Dimethylsulfoxide as Cryoprotectants of Japanese White Rabbit Spermatozoa
The Journal of reproduction and development, 2006Co-Authors: Naomi Kashiwazaki, Yasushi Okuda, Yasunari Seita, Shin Hisamatsu, Shigenori Sonoki, Masao Shino, Toshio Masaoka, Tomo InomataAbstract:The Rabbit is considered to be a valuable laboratory animal. We compared glycerol, lactamide, acetamide, and dimethylsulfoxide (DMSO) as cryoprotectants in egg-yolk diluent of ejaculated Japanese White Rabbit spermatozoa for improvement of sperm cryopreservation methods. Rabbit semen was frozen with 1.0 M glycerol, lactamide, acetamide, or DMSO in plastic straws. Forward progressive motility and plasma membrane integrity of the post-thaw spermatozoa were examined. The rate of forward progressive motile spermatozoa in lactamide (37.8 +/- 3.0%) was significantly (P
Naomi Kashiwazaki - One of the best experts on this subject based on the ideXlab platform.
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fertility of spermatozoa cryopreserved with 2 acetamide or glycerol through artificial insemination in the Japanese White Rabbit
Experimental Animals, 2007Co-Authors: Yasushi Okuda, Yasunari Seita, Shin Hisamatsu, Shigenori Sonoki, Masao Shino, Toshio Masaoka, Tomo Inomata, Shinichi Kamijo, Naomi KashiwazakiAbstract:The Rabbit is considered to be a valuable laboratory animal. We compared 2% acetamide and glycerol as cryoprotectants in egg-yolk diluent for ejaculated Japanese White Rabbit spermatozoa to improve sperm cryopreservation methods. Fertility through artificial insemination, forward progressive motility and plasma membrane integrity of the post-thaw spermatozoa were examined. The rates of forward progressive motility and plasma membrane integrity of the spermatozoa frozen with acetamide (27.1 ± 8.3% and 24.5 ± 6.5%) were significantly (P<0.05) higher than those of the spermatozoa frozen with glycerol (16.3 ± 10.9% and 14.3 ± 7.6%). Though there was no significant difference in the kindling rates, the litter size of females inseminated with spermatozoa frozen with acetamide (6.0 ± 1.1) were significantly (P<0.05) higher than those of spermatozoa frozen with glycerol (3.0 ± 0.4). The results indicate that 2% acetamide has a higher cryoprotective effect than 2% glycerol for sperm cryopreservation in the Japanese White Rabbit.
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Fertility of spermatozoa cryopreserved with 2% acetamide or glycerol through artificial insemination in the Japanese White Rabbit.
Experimental animals, 2007Co-Authors: Yasushi Okuda, Yasunari Seita, Shin Hisamatsu, Shigenori Sonoki, Masao Shino, Toshio Masaoka, Tomo Inomata, Shinichi Kamijo, Naomi KashiwazakiAbstract:The Rabbit is considered to be a valuable laboratory animal. We compared 2% acetamide and glycerol as cryoprotectants in egg-yolk diluent for ejaculated Japanese White Rabbit spermatozoa to improve sperm cryopreservation methods. Fertility through artificial insemination, forward progressive motility and plasma membrane integrity of the post-thaw spermatozoa were examined. The rates of forward progressive motility and plasma membrane integrity of the spermatozoa frozen with acetamide (27.1 ± 8.3% and 24.5 ± 6.5%) were significantly (P
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comparison of glycerol lactamide acetamide and dimethylsulfoxide as cryoprotectants of Japanese White Rabbit spermatozoa
Journal of Reproduction and Development, 2006Co-Authors: Naomi Kashiwazaki, Yasushi Okuda, Yasunari Seita, Shin Hisamatsu, Shigenori Sonoki, Masao Shino, Toshio Masaoka, Tomo InomataAbstract:The Rabbit is considered to be a valuable laboratory animal. We compared glycerol, lactamide, acetamide, and dimethylsulfoxide (DMSO) as cryoprotectants in egg-yolk diluent of ejaculated Japanese White Rabbit spermatozoa for improvement of sperm cryopreservation methods. Rabbit semen was frozen with 1.0 M glycerol, lactamide, acetamide, or DMSO in plastic straws. Forward progressive motility and plasma membrane integrity of the post-thaw spermatozoa were examined. The rate of forward progressive motile spermatozoa in lactamide (37.8 +/- 3.0%) was significantly (P<0.05) higher than in glycerol (17.0 +/- 3.3%). In addition, the rates of sperm plasma membrane integrity in lactamide and acetamide (35.9 +/- 3.3% and 30.2 +/- 3.0%, respectively) were significantly (P<0.05) higher than in glycerol (17.0 +/- 2.6%). The results indicate that 1.0 M lactamide and acetamide have higher cryoprotective effects than 1.0 M glycerol for cryopreservation of Japanese White Rabbit spermatozoa.
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Comparison of Glycerol, Lactamide, Acetamide and Dimethylsulfoxide as Cryoprotectants of Japanese White Rabbit Spermatozoa
The Journal of reproduction and development, 2006Co-Authors: Naomi Kashiwazaki, Yasushi Okuda, Yasunari Seita, Shin Hisamatsu, Shigenori Sonoki, Masao Shino, Toshio Masaoka, Tomo InomataAbstract:The Rabbit is considered to be a valuable laboratory animal. We compared glycerol, lactamide, acetamide, and dimethylsulfoxide (DMSO) as cryoprotectants in egg-yolk diluent of ejaculated Japanese White Rabbit spermatozoa for improvement of sperm cryopreservation methods. Rabbit semen was frozen with 1.0 M glycerol, lactamide, acetamide, or DMSO in plastic straws. Forward progressive motility and plasma membrane integrity of the post-thaw spermatozoa were examined. The rate of forward progressive motile spermatozoa in lactamide (37.8 +/- 3.0%) was significantly (P
Yoichi Takano - One of the best experts on this subject based on the ideXlab platform.
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The efficacy of slide tracheoplasty in a growing Rabbit model
Pediatric surgery international, 2011Co-Authors: Yoichi Takano, Kosaku Maeda, Chieko Hisamatsu, Eiji NishijimaAbstract:Purpose Slide tracheoplasty for congenital tracheal stenosis was reported by Tsang. This procedure provides good results, but there are few studies about the tracheal growth of post-surgical condition. Recently, not only traditional posterior to anterior slide plasty but also right side to left side slide plasty was reported. We evaluated the tracheal growth after slide tracheoplasty in growing Japanese White Rabbit model.
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ORIGINAL ARTICLE The efficacy of slide tracheoplasty in a growing Rabbit model
2011Co-Authors: Yoichi Takano, Kosaku Maeda, Chieko Hisamatsu, Eiji NishijimaAbstract:Ó The Author(s) 2011. This article is published with open access at Springerlink.com Purpose Slide tracheoplasty for congenital tracheal stenosis was reported by Tsang. This procedure provides good results, but there are few studies about the tracheal growth of post-surgical condition. Recently, not only traditional posterior to anterior slide plasty but also right side to left side slide plasty was reported. We evaluated the tracheal growth after slide tracheoplasty in growing Japanese White Rabbit model. Methods The Japanese White Rabbits, weighing 1,600– 2,400 g, were used for this study. One-third of the estimated length of the trachea was slantingly resected about an appropriate angle. We performed a slant, side-to-side tracheal anastomosis in two ways (slanted from the front to the back in four animals, and slanted from right to left in four animals) on growing Rabbits as an animal model of slide tracheoplasty. Results All the Rabbits were alive until the 10th week after surgery. There was no evidence of differential growth in any part of the circumference of the normal and anastomotic tracheas. Histologically, all tracheal lumina were completely lined with normal respiratory epithelium and normal surrounding cartilage
Kosaku Maeda - One of the best experts on this subject based on the ideXlab platform.
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The efficacy of slide tracheoplasty in a growing Rabbit model
Pediatric surgery international, 2011Co-Authors: Yoichi Takano, Kosaku Maeda, Chieko Hisamatsu, Eiji NishijimaAbstract:Purpose Slide tracheoplasty for congenital tracheal stenosis was reported by Tsang. This procedure provides good results, but there are few studies about the tracheal growth of post-surgical condition. Recently, not only traditional posterior to anterior slide plasty but also right side to left side slide plasty was reported. We evaluated the tracheal growth after slide tracheoplasty in growing Japanese White Rabbit model.
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ORIGINAL ARTICLE The efficacy of slide tracheoplasty in a growing Rabbit model
2011Co-Authors: Yoichi Takano, Kosaku Maeda, Chieko Hisamatsu, Eiji NishijimaAbstract:Ó The Author(s) 2011. This article is published with open access at Springerlink.com Purpose Slide tracheoplasty for congenital tracheal stenosis was reported by Tsang. This procedure provides good results, but there are few studies about the tracheal growth of post-surgical condition. Recently, not only traditional posterior to anterior slide plasty but also right side to left side slide plasty was reported. We evaluated the tracheal growth after slide tracheoplasty in growing Japanese White Rabbit model. Methods The Japanese White Rabbits, weighing 1,600– 2,400 g, were used for this study. One-third of the estimated length of the trachea was slantingly resected about an appropriate angle. We performed a slant, side-to-side tracheal anastomosis in two ways (slanted from the front to the back in four animals, and slanted from right to left in four animals) on growing Rabbits as an animal model of slide tracheoplasty. Results All the Rabbits were alive until the 10th week after surgery. There was no evidence of differential growth in any part of the circumference of the normal and anastomotic tracheas. Histologically, all tracheal lumina were completely lined with normal respiratory epithelium and normal surrounding cartilage
Yasushi Okuda - One of the best experts on this subject based on the ideXlab platform.
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fertility of spermatozoa cryopreserved with 2 acetamide or glycerol through artificial insemination in the Japanese White Rabbit
Experimental Animals, 2007Co-Authors: Yasushi Okuda, Yasunari Seita, Shin Hisamatsu, Shigenori Sonoki, Masao Shino, Toshio Masaoka, Tomo Inomata, Shinichi Kamijo, Naomi KashiwazakiAbstract:The Rabbit is considered to be a valuable laboratory animal. We compared 2% acetamide and glycerol as cryoprotectants in egg-yolk diluent for ejaculated Japanese White Rabbit spermatozoa to improve sperm cryopreservation methods. Fertility through artificial insemination, forward progressive motility and plasma membrane integrity of the post-thaw spermatozoa were examined. The rates of forward progressive motility and plasma membrane integrity of the spermatozoa frozen with acetamide (27.1 ± 8.3% and 24.5 ± 6.5%) were significantly (P<0.05) higher than those of the spermatozoa frozen with glycerol (16.3 ± 10.9% and 14.3 ± 7.6%). Though there was no significant difference in the kindling rates, the litter size of females inseminated with spermatozoa frozen with acetamide (6.0 ± 1.1) were significantly (P<0.05) higher than those of spermatozoa frozen with glycerol (3.0 ± 0.4). The results indicate that 2% acetamide has a higher cryoprotective effect than 2% glycerol for sperm cryopreservation in the Japanese White Rabbit.
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Fertility of spermatozoa cryopreserved with 2% acetamide or glycerol through artificial insemination in the Japanese White Rabbit.
Experimental animals, 2007Co-Authors: Yasushi Okuda, Yasunari Seita, Shin Hisamatsu, Shigenori Sonoki, Masao Shino, Toshio Masaoka, Tomo Inomata, Shinichi Kamijo, Naomi KashiwazakiAbstract:The Rabbit is considered to be a valuable laboratory animal. We compared 2% acetamide and glycerol as cryoprotectants in egg-yolk diluent for ejaculated Japanese White Rabbit spermatozoa to improve sperm cryopreservation methods. Fertility through artificial insemination, forward progressive motility and plasma membrane integrity of the post-thaw spermatozoa were examined. The rates of forward progressive motility and plasma membrane integrity of the spermatozoa frozen with acetamide (27.1 ± 8.3% and 24.5 ± 6.5%) were significantly (P
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comparison of glycerol lactamide acetamide and dimethylsulfoxide as cryoprotectants of Japanese White Rabbit spermatozoa
Journal of Reproduction and Development, 2006Co-Authors: Naomi Kashiwazaki, Yasushi Okuda, Yasunari Seita, Shin Hisamatsu, Shigenori Sonoki, Masao Shino, Toshio Masaoka, Tomo InomataAbstract:The Rabbit is considered to be a valuable laboratory animal. We compared glycerol, lactamide, acetamide, and dimethylsulfoxide (DMSO) as cryoprotectants in egg-yolk diluent of ejaculated Japanese White Rabbit spermatozoa for improvement of sperm cryopreservation methods. Rabbit semen was frozen with 1.0 M glycerol, lactamide, acetamide, or DMSO in plastic straws. Forward progressive motility and plasma membrane integrity of the post-thaw spermatozoa were examined. The rate of forward progressive motile spermatozoa in lactamide (37.8 +/- 3.0%) was significantly (P<0.05) higher than in glycerol (17.0 +/- 3.3%). In addition, the rates of sperm plasma membrane integrity in lactamide and acetamide (35.9 +/- 3.3% and 30.2 +/- 3.0%, respectively) were significantly (P<0.05) higher than in glycerol (17.0 +/- 2.6%). The results indicate that 1.0 M lactamide and acetamide have higher cryoprotective effects than 1.0 M glycerol for cryopreservation of Japanese White Rabbit spermatozoa.
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Comparison of Glycerol, Lactamide, Acetamide and Dimethylsulfoxide as Cryoprotectants of Japanese White Rabbit Spermatozoa
The Journal of reproduction and development, 2006Co-Authors: Naomi Kashiwazaki, Yasushi Okuda, Yasunari Seita, Shin Hisamatsu, Shigenori Sonoki, Masao Shino, Toshio Masaoka, Tomo InomataAbstract:The Rabbit is considered to be a valuable laboratory animal. We compared glycerol, lactamide, acetamide, and dimethylsulfoxide (DMSO) as cryoprotectants in egg-yolk diluent of ejaculated Japanese White Rabbit spermatozoa for improvement of sperm cryopreservation methods. Rabbit semen was frozen with 1.0 M glycerol, lactamide, acetamide, or DMSO in plastic straws. Forward progressive motility and plasma membrane integrity of the post-thaw spermatozoa were examined. The rate of forward progressive motile spermatozoa in lactamide (37.8 +/- 3.0%) was significantly (P
