The Experts below are selected from a list of 288 Experts worldwide ranked by ideXlab platform
Guoping Chen - One of the best experts on this subject based on the ideXlab platform.
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biochemical and molecular analysis of a temperature sensitive albino mutant in Kale named white dove
Plant Growth Regulation, 2013Co-Authors: Shuang Zhou, Bin Zhang, Mingku Zhu, Lei Deng, Yu Pan, Guoping ChenAbstract:“White Dove” is a mutant in Kale (Brassica oleracea var. acephala f. tricolor), which exhibits a mutant albino phenotype in the interior of the plant under low temperature conditions. Chlorophyll content in “White Dove” was dramatically reduced under low temperature conditions, while the content in “Green Dove” decreased slightly under the same conditions. The levels of five chlorophyll precursors suggested that chlorophyll biosynthesis in white Kale was inhibited by low temperature stress at the step of Pchlide. However, Mg-Proto IX was not inhibited in white Kale grown under low temperature conditions. The results of quantitative RT-PCR illustrated that the chlorophyll biosynthetic genes in the white cultivar were dramatically down-regulated by low temperature stress from the step of POR, while CISC and DBB1B in the white cultivar were dramatically induced under low temperature conditions. The results of transmission electron microscopy analysis showed that there were normal chloroplasts in the young leaves of white Kale grown at 20 °C, whereas proplastids were observed in white Kale grown at 5 °C. These results strongly suggested that low-temperature stress significantly inhibited plastid development in the young leaves of white Kale, and repressed chlorophyll biosynthesis at the step of Pchlide by down-regulating the expression of downstream chlorophyll biosynthetic genes, resulting in undifferentiated proplastids and the albino phenotype observed in young leaves. Several genes associated with chlorophyll accumulation were also affected by low temperature conditions in white Kale, especially CISC and DBB1B.
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a putative functional myb transcription factor induced by low temperature regulates anthocyanin biosynthesis in purple Kale brassica oleracea var acephala f tricolor
Plant Cell Reports, 2012Co-Authors: Bin Zhang, Zongli Hu, Yanjie Zhang, Yali Li, Shuang Zhou, Guoping ChenAbstract:The purple Kale (Brassica Oleracea var. acephala f. tricolor) is a mutation in Kales, giving the mutant phenotype of brilliant purple color in the interior. Total anthocyanin analysis showed that the amount of anthocyanins in the purple Kale was up to 1.73 mg g−1 while no anthocyanin was detected in the white Kale. To elucidate the molecular mechanism of the anthocyanin biosynthesis in the purple Kale, we analyzed the expression of structural genes and some transcription factors associated with anthocyanin biosynthesis in the purple cultivar “Red Dove” and the white cultivar “White Dove”. The result showed that nearly all the anthocyanin biosynthetic genes showed higher expression levels in the purple cultivar than in the white cultivar, especially for DFR and ANS, they were barely detected in the white cultivar. Interestingly, the fact that a R2R3 MYB transcription factor named BoPAP1 was extremely up-regulated in the purple Kale and induced by low temperature attracted our attention. Further sequence analysis showed that BoPAP1 shared high similarity with AtPAP1 and BoMYB1. In addition, the anthocyanin accumulation in the purple Kale is strongly induced by the low temperature stress. The total anthocyanin contents in the purple Kale under low temperature were about 50-fold higher than the plants grown in the greenhouse. The expression of anthocyanin biosynthetic genes C4H, F3H, DFR, ANS and UFGT were all enhanced under the low temperature. These evidences strongly suggest that BoPAP1 may play an important role in activating the anthocyanin structural genes for the abundant anthocyanin accumulation in the purple Kale.
Nikolai Kuhnert - One of the best experts on this subject based on the ideXlab platform.
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changes in low molecular weight carbohydrates in Kale during development and acclimation to cold temperatures determined by chromatographic techniques coupled to mass spectrometry
Food Research International, 2020Co-Authors: Roberto Megiasperez, Christoph Hahn, Ana I Ruizmatute, Britta Behrends, Dirk C Albach, Nikolai KuhnertAbstract:Abstract Kale (Brassica oleracea) is a cool-season vegetable widely employed in the elaboration of diverse products such as tea and smoothies in USA or in the Northern German cuisine in wintertime. Besides, Kale is gaining attention due to the diverse health benefits reported in the literature for its consumption, e.g. antigenotoxic and anticarcinogenic effect, protection of cardiovascular system and gastrointestinal tract. Low molecular weight carbohydrates (LMWC) are compounds directly related with Kale taste, flavour and nutrition quality. Despite different studies focusing on the chemical composition of Kale, few information on LMWC is available. Thus, in this work a multianalytical approach was conducted in order to perform a comprehensive study of Kale LMWC; their evolution during plant development and acclimation to cool temperatures was also evaluated. Gas chromatography coupled to mass spectrometry allowed the identification of 13 LMWC, being myo-inositol, galactinol, maltose and melibiose described for the first time in Kale. Eight major LMWC were quantified in three different commercial Kale cultivars using hydrophilic interaction liquid chromatography coupled to mass spectrometry to monitor possible differences in their content during plant development and as consequence of plant acclimation to cold temperatures. Overall, for all types of Kales under study, the content of maltose and sucrose decreased during the plant development while the concentration of fructose, melibiose, maltose, raffinose and galactinol was increased in all Kale types exposed to low temperatures. These results underline the importance of controlling the temperature during Kale cultivation in order to obtain products with a high nutritional value.
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Diversity of Kale (Brassica oleracea var. sabellica): Glucosinolate Content and Phylogenetic Relationships.
Journal of agricultural and food chemistry, 2016Co-Authors: Christoph Hahn, Nikolai Kuhnert, Anja Müller, Dirk C AlbachAbstract:Recently, Kale has become popular due to nutritive components beneficial for human health. It is an important source of phytochemicals such as glucosinolates that trigger associated cancer-preventive activity. However, nutritional value varies among glucosinolates and among cultivars. Here, we start a systematic determination of the content of five glucosinolates in 25 Kale varieties and 11 non-Kale Brassica oleracea cultivars by HPLC-DAD-ESI-MSn and compare the profiles with results from the analysis of SNPs derived from a KASP genotyping assay. Our results demonstrate that the glucosinolate levels differ markedly among varieties of different origin. Comparison of the phytochemical data with phylogenetic relationships revealed that the common name Kale refers to at least three different groups. German, American, and Italian Kales differ morphologically and phytochemically. Landraces do not show outstanding glucosinolate levels. Our results demonstrate the diversity of Kale and the importance of preservin...
Chenghuan Yan - One of the best experts on this subject based on the ideXlab platform.
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Rapid identification of the purple stem (Ps) gene of Chinese Kale (Brassica oleracea var. alboglabra) in a segregation distortion population by bulked segregant analysis and RNA sequencing
Molecular Breeding, 2017Co-Authors: Qiwei Tang, Guanghui An, Jiongjiong Chen, Mengyu Tian, Weiyi Zhang, Chenghuan YanAbstract:Abundant anthocyanins accumulate in many flowers, leaves, fruits, as well as stems. In this study, an F2 segregating population derived from a cross between Chinese Kale with a purple stem and Kale with a green stem was used to decipher the genetic basis for the stem color. Bulked segregant analysis in combination with RNA sequencing (BSR-seq) was used to genetically map the causal gene for the purple stem (Ps), and the allele frequency difference between the two pools showed a single peak on chromosome C09. Ps was fine-mapped to the 0.32 cM interval with 406 kb sequences. A gene-encoding dihydroflavonol 4-reductase (DFR), an enzyme in the anthocyanin biosynthesis pathway, was differentially expressed between the two pools, and was identified as the candidate gene for the purple stem in Chinese Kale. Sequence analysis revealed that 1 bp was inserted in the coding sequence of exon 2 of the BoDRF gene in green Kale, causing frameshift and loss of function. The expression level of the BoDFR gene was significantly lower than the Chinese Kale cultivar with a purple stem. The genetic results of the Ps gene will be useful for future development of purple vegetables.
Dirk C Albach - One of the best experts on this subject based on the ideXlab platform.
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changes in low molecular weight carbohydrates in Kale during development and acclimation to cold temperatures determined by chromatographic techniques coupled to mass spectrometry
Food Research International, 2020Co-Authors: Roberto Megiasperez, Christoph Hahn, Ana I Ruizmatute, Britta Behrends, Dirk C Albach, Nikolai KuhnertAbstract:Abstract Kale (Brassica oleracea) is a cool-season vegetable widely employed in the elaboration of diverse products such as tea and smoothies in USA or in the Northern German cuisine in wintertime. Besides, Kale is gaining attention due to the diverse health benefits reported in the literature for its consumption, e.g. antigenotoxic and anticarcinogenic effect, protection of cardiovascular system and gastrointestinal tract. Low molecular weight carbohydrates (LMWC) are compounds directly related with Kale taste, flavour and nutrition quality. Despite different studies focusing on the chemical composition of Kale, few information on LMWC is available. Thus, in this work a multianalytical approach was conducted in order to perform a comprehensive study of Kale LMWC; their evolution during plant development and acclimation to cool temperatures was also evaluated. Gas chromatography coupled to mass spectrometry allowed the identification of 13 LMWC, being myo-inositol, galactinol, maltose and melibiose described for the first time in Kale. Eight major LMWC were quantified in three different commercial Kale cultivars using hydrophilic interaction liquid chromatography coupled to mass spectrometry to monitor possible differences in their content during plant development and as consequence of plant acclimation to cold temperatures. Overall, for all types of Kales under study, the content of maltose and sucrose decreased during the plant development while the concentration of fructose, melibiose, maltose, raffinose and galactinol was increased in all Kale types exposed to low temperatures. These results underline the importance of controlling the temperature during Kale cultivation in order to obtain products with a high nutritional value.
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Diversity of Kale (Brassica oleracea var. sabellica): Glucosinolate Content and Phylogenetic Relationships.
Journal of agricultural and food chemistry, 2016Co-Authors: Christoph Hahn, Nikolai Kuhnert, Anja Müller, Dirk C AlbachAbstract:Recently, Kale has become popular due to nutritive components beneficial for human health. It is an important source of phytochemicals such as glucosinolates that trigger associated cancer-preventive activity. However, nutritional value varies among glucosinolates and among cultivars. Here, we start a systematic determination of the content of five glucosinolates in 25 Kale varieties and 11 non-Kale Brassica oleracea cultivars by HPLC-DAD-ESI-MSn and compare the profiles with results from the analysis of SNPs derived from a KASP genotyping assay. Our results demonstrate that the glucosinolate levels differ markedly among varieties of different origin. Comparison of the phytochemical data with phylogenetic relationships revealed that the common name Kale refers to at least three different groups. German, American, and Italian Kales differ morphologically and phytochemically. Landraces do not show outstanding glucosinolate levels. Our results demonstrate the diversity of Kale and the importance of preservin...
Jianjun Lei - One of the best experts on this subject based on the ideXlab platform.
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Cloning and Expression Analysis of the BocMBF1c Gene Involved in Heat Tolerance in Chinese Kale.
International journal of molecular sciences, 2019Co-Authors: Zou Lifang, Bihao Cao, Guoju Chen, Changming Chen, Jianjun LeiAbstract:Chinese Kale (Brassica oleracea var. chinensis Lei) is an important vegetable crop in South China, valued for its nutritional content and taste. Nonetheless, the thermal tolerance of Chinese Kale still needs improvement. Molecular characterization of Chinese Kale’s heat stress response could provide a timely solution for developing a thermally tolerant Chinese Kale variety. Here, we report the cloning of multi-protein bridging factor (MBF) 1c from Chinese Kale (BocMBF1c), an ortholog to the key heat stress responsive gene MBF1c. Phylogenetic analysis showed that BocMBF1c is highly similar to the stress-response transcriptional coactivator MBF1c from Arabidopsis thaliana (AtMBF1c), and the BocMBF1c coding region conserves MBF1 and helix-turn-helix (HTH) domains. Moreover, the promoter region of BocMBF1c contains three heat shock elements (HSEs) and, thus, is highly responsive to heat treatment. This was verified in Nicotiana benthamiana leaf tissue using a green fluorescent protein (GFP) reporter. In addition, the expression of BocMBF1c can be induced by various abiotic stresses in Chinese Kale which indicates the involvement of stress responses. The BocMBF1c-eGFP (enhanced green fluorescent protein) chimeric protein quickly translocated into the nucleus under high temperature treatment in Nicotiana benthamiana leaf tissue. Overexpression of BocMBF1c in Arabidopsis thaliana results in a larger size and enhanced thermal tolerance compared with the wild type. Our results provide valuable insight for the role of BocMBF1c during heat stress in Chinese Kale.
