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Jack L Summers - One of the best experts on this subject based on the ideXlab platform.
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apatone induces endometrioid ovarian carcinoma mdah 2774 cells to undergo Karyolysis and cell death by autoschizis a potent and safe anticancer treatment
Translational Research in Anatomy, 2015Co-Authors: Jacques Gilloteaux, Lee H Lau, Ioulia Gourari, Deborah R Neal, James M Jamison, Jack L SummersAbstract:Abstract Ovarian cancers are still the most lethal gynecologic malignancy. As a novel strategy against this poor outcome cytotoxic alterations induced by a pro-oxidant treatment on human ovarian endometrioid carcinoma (MDAH 2774) cells are revisited by using light, scanning and transmission electron microscopy. A series of sequential and concomitant cellular and organelle injuries induced by ascorbate: menadione combination (VC: VK3) or Apatone® is emphasized. This adjuvant or treatment is able to kill majority of these tumor cells through ‘autoschizic cell death’, a mode of cell death different than apoptosis. Autoschizic cell death is significant after a short period of treatment to decrease the ovarian tumor cell population through induced injuries that proceed from membranes to most organelles: Karyolysis with nucleolar segregation and fragmentation, autophagy of mitochondria, lysosome and other organelles as well as cytoskeletal defects. The cytoskeletal damages are evidenced by morphology changes that included auto- or self-excised pieces of cytoplasm lacking organelles apparently facilitated by grouping of vacuolated endoplasm. These results obtained against this endometrioid ovary cell line are comforted by other studies using Apatone® against other carcinomas in vitro and in vivo. Altogether these reports support Apatone® as a new drug that can favorably be used as a novel potent, safe, and inexpensive clinical adjuvant or treatment against ovarian cancers.
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Apatone® induces endometrioid ovarian carcinoma (MDAH 2774) cells to undergo Karyolysis and cell death by autoschizis: A potent and safe anticancer treatment
Elsevier, 2015Co-Authors: Jacques Gilloteaux, Lee H Lau, Ioulia Gourari, James M Jamison, Deborah Neal, Jack L SummersAbstract:Ovarian cancers are still the most lethal gynecologic malignancy. As a novel strategy against this poor outcome cytotoxic alterations induced by a pro-oxidant treatment on human ovarian endometrioid carcinoma (MDAH 2774) cells are revisited by using light, scanning and transmission electron microscopy. A series of sequential and concomitant cellular and organelle injuries induced by ascorbate: menadione combination (VC: VK3) or Apatone® is emphasized. This adjuvant or treatment is able to kill majority of these tumor cells through ‘autoschizic cell death’, a mode of cell death different than apoptosis. Autoschizic cell death is significant after a short period of treatment to decrease the ovarian tumor cell population through induced injuries that proceed from membranes to most organelles: Karyolysis with nucleolar segregation and fragmentation, autophagy of mitochondria, lysosome and other organelles as well as cytoskeletal defects. The cytoskeletal damages are evidenced by morphology changes that included auto- or self-excised pieces of cytoplasm lacking organelles apparently facilitated by grouping of vacuolated endoplasm. These results obtained against this endometrioid ovary cell line are comforted by other studies using Apatone® against other carcinomas in vitro and in vivo. Altogether these reports support Apatone® as a new drug that can favorably be used as a novel potent, safe, and inexpensive clinical adjuvant or treatment against ovarian cancers. Keywords: Ascorbate, Menadione, Endometrioid ovarian cancer MDAH 2774, Autoschizis cell death, DN
Jacques Gilloteaux - One of the best experts on this subject based on the ideXlab platform.
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apatone induces endometrioid ovarian carcinoma mdah 2774 cells to undergo Karyolysis and cell death by autoschizis a potent and safe anticancer treatment
Translational Research in Anatomy, 2015Co-Authors: Jacques Gilloteaux, Lee H Lau, Ioulia Gourari, Deborah R Neal, James M Jamison, Jack L SummersAbstract:Abstract Ovarian cancers are still the most lethal gynecologic malignancy. As a novel strategy against this poor outcome cytotoxic alterations induced by a pro-oxidant treatment on human ovarian endometrioid carcinoma (MDAH 2774) cells are revisited by using light, scanning and transmission electron microscopy. A series of sequential and concomitant cellular and organelle injuries induced by ascorbate: menadione combination (VC: VK3) or Apatone® is emphasized. This adjuvant or treatment is able to kill majority of these tumor cells through ‘autoschizic cell death’, a mode of cell death different than apoptosis. Autoschizic cell death is significant after a short period of treatment to decrease the ovarian tumor cell population through induced injuries that proceed from membranes to most organelles: Karyolysis with nucleolar segregation and fragmentation, autophagy of mitochondria, lysosome and other organelles as well as cytoskeletal defects. The cytoskeletal damages are evidenced by morphology changes that included auto- or self-excised pieces of cytoplasm lacking organelles apparently facilitated by grouping of vacuolated endoplasm. These results obtained against this endometrioid ovary cell line are comforted by other studies using Apatone® against other carcinomas in vitro and in vivo. Altogether these reports support Apatone® as a new drug that can favorably be used as a novel potent, safe, and inexpensive clinical adjuvant or treatment against ovarian cancers.
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Apatone® induces endometrioid ovarian carcinoma (MDAH 2774) cells to undergo Karyolysis and cell death by autoschizis: A potent and safe anticancer treatment
Elsevier, 2015Co-Authors: Jacques Gilloteaux, Lee H Lau, Ioulia Gourari, James M Jamison, Deborah Neal, Jack L SummersAbstract:Ovarian cancers are still the most lethal gynecologic malignancy. As a novel strategy against this poor outcome cytotoxic alterations induced by a pro-oxidant treatment on human ovarian endometrioid carcinoma (MDAH 2774) cells are revisited by using light, scanning and transmission electron microscopy. A series of sequential and concomitant cellular and organelle injuries induced by ascorbate: menadione combination (VC: VK3) or Apatone® is emphasized. This adjuvant or treatment is able to kill majority of these tumor cells through ‘autoschizic cell death’, a mode of cell death different than apoptosis. Autoschizic cell death is significant after a short period of treatment to decrease the ovarian tumor cell population through induced injuries that proceed from membranes to most organelles: Karyolysis with nucleolar segregation and fragmentation, autophagy of mitochondria, lysosome and other organelles as well as cytoskeletal defects. The cytoskeletal damages are evidenced by morphology changes that included auto- or self-excised pieces of cytoplasm lacking organelles apparently facilitated by grouping of vacuolated endoplasm. These results obtained against this endometrioid ovary cell line are comforted by other studies using Apatone® against other carcinomas in vitro and in vivo. Altogether these reports support Apatone® as a new drug that can favorably be used as a novel potent, safe, and inexpensive clinical adjuvant or treatment against ovarian cancers. Keywords: Ascorbate, Menadione, Endometrioid ovarian cancer MDAH 2774, Autoschizis cell death, DN
Erhan Ünlü - One of the best experts on this subject based on the ideXlab platform.
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histopathological changes in the gill and liver tissues of freshwater fish cirrhinus mrigala exposed to dichlorvos
Brazilian Archives of Biology and Technology, 2009Co-Authors: Babu Velmurugan, Michael Selvanayagam, Elif Ipek Cengiz, Erhan ÜnlüAbstract:The histopathological effects of dichlorvos, an organophosphate pesticide, on the gill and liver tissues in Cirrhinus mrigala were determined by light microscopy. The fishes were exposed to sublethal concentrations (0.91 and 1.82 ppm) of dichlorvos for 10 days with parallel untreated control. No histopathological effects were observed in control group. Hyperplasia, desquamation, and necrosis of epithelial, epithelial lifting, oedema, lamellar fusion, collapsed secondary lamellae, curling of secondary lamellae and aneurism in the secondary lamellae were observed in gill tissues exposed to dichlorvos. Hepatic lesions in the liver tissues of fishes exposed to dichlorvos were characterized by cloudy swelling of hepatocytes, congestion, vacuolar degeneration, Karyolysis, karyohexis, dilation of sinusoids and nuclear hypertrophy.
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histopathology of lambda cyhalothrin on tissues gill kidney liver and intestine of cirrhinus mrigala
Environmental Toxicology and Pharmacology, 2007Co-Authors: Babu Velmurugan, Michael Selvanayagam, Elif Ipek Cengiz, Erhan ÜnlüAbstract:The histopathology of lambda-cyhalothrin, a synthetic pyrethroid pesticide, on the gill, liver, intestine and kidney tissues in fish, mrigal (Cirrhinus mrigala), which is one of Indian major carp species were determined by light microscopy. The fish were experimentally exposed to sub-lethal concentrations (0.3 ppb and 0.6 ppb) of lambda-cyhalothrin for 10 days. Tissues were normal in the control group. Epithelial hyperplasia, aneurism, epithelial necrosis, desquamation, epithelial lifting, oedema, shortening of secondary lamellae and lamellar fusion were observed in gill tissues exposed to lambda-cyhalothrin. Necrosis of tubular epithelium, cloudy swelling of epithelial cells of renal tubules, narrowing of the tubular lumen, contraction of the glomerulus and expansion of space inside the Bowman’s capsule were observed in the kidney tissues of fish after exposure. Hepatic lesions in fish exposed to lambda-cyhalothrin were characterized by hypertrophy of hepatocytes, cloudy degeneration, congestion, Karyolysis, karyohexis, dilatation of sinusoids and focal necrosis. The intestinal lesions included infiltration of eosinophils into the lamina propria and atrophy of epithelial cells. The present study proves its toxic potential in terms of the damages induced by lambda-cyhalothrin in organ level. In natural condition lambda-cyhalothrin will be less than the present study, but continuous usage of the pesticide might lead to the concentration that was used in the experimental condition. © 2007 Published by Elsevier B.V.
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histopathology of lambda cyhalothrin on tissues gill kidney liver and intestine of cirrhinus mrigala
Environmental Toxicology and Pharmacology, 2007Co-Authors: Babu Velmurugan, Michael Selvanayagam, Elif Ipek Cengiz, Erhan ÜnlüAbstract:The histopathology of lambda-cyhalothrin, a synthetic pyrethroid pesticide, on the gill, liver, intestine and kidney tissues in fish, mrigal (Cirrhinus mrigala), which is one of Indian major carp species were determined by light microscopy. The fish were experimentally exposed to sub-lethal concentrations (0.3 ppb and 0.6 ppb) of lambda-cyhalothrin for 10 days. Tissues were normal in the control group. Epithelial hyperplasia, aneurism, epithelial necrosis, desquamation, epithelial lifting, oedema, shortening of secondary lamellae and lamellar fusion were observed in gill tissues exposed to lambda-cyhalothrin. Necrosis of tubular epithelium, cloudy swelling of epithelial cells of renal tubules, narrowing of the tubular lumen, contraction of the glomerulus and expansion of space inside the Bowman’s capsule were observed in the kidney tissues of fish after exposure. Hepatic lesions in fish exposed to lambda-cyhalothrin were characterized by hypertrophy of hepatocytes, cloudy degeneration, congestion, Karyolysis, karyohexis, dilatation of sinusoids and focal necrosis. The intestinal lesions included infiltration of eosinophils into the lamina propria and atrophy of epithelial cells. The present study proves its toxic potential in terms of the damages induced by lambda-cyhalothrin in organ level. In natural condition lambda-cyhalothrin will be less than the present study, but continuous usage of the pesticide might lead to the concentration that was used in the experimental condition. © 2007 Published by Elsevier B.V.
Serap Ergenegozukara - One of the best experts on this subject based on the ideXlab platform.
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cytogenetic biomonitoring in petrol station attendants micronucleus test in exfoliated buccal cells
Mutagenesis, 2003Co-Authors: Ayla Celik, Tolga Cavas, Serap ErgenegozukaraAbstract:To study the effects of occupational exposure to petroleum derivates such as benzene, exfoliated buccal cells from 50 petrol station attendants and 50 age- and sex-matched control subjects were examined for micronucleus (MN) frequency. Frequencies of nuclear abnormalities (NA) other than micronuclei, such as binucleates, karyorrhexis and Karyolysis, were also evaluated. Benzene exposure was ascertained by measuring urinary phenol levels. The mean urinary phenol level of station workers was found to be significantly higher than that of control subjects (P < 0.05). Analysis of buccal cells revealed that MN and NA frequencies in petrol station workers were significantly higher than in control subjects (P < 0.01) and also significantly related to smoking habit (P < 0.01). Our findings indicate that the petrol station workers are under risk of significant cytogenetic damage.
Babu Velmurugan - One of the best experts on this subject based on the ideXlab platform.
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histopathological changes in the gill and liver tissues of freshwater fish cirrhinus mrigala exposed to dichlorvos
Brazilian Archives of Biology and Technology, 2009Co-Authors: Babu Velmurugan, Michael Selvanayagam, Elif Ipek Cengiz, Erhan ÜnlüAbstract:The histopathological effects of dichlorvos, an organophosphate pesticide, on the gill and liver tissues in Cirrhinus mrigala were determined by light microscopy. The fishes were exposed to sublethal concentrations (0.91 and 1.82 ppm) of dichlorvos for 10 days with parallel untreated control. No histopathological effects were observed in control group. Hyperplasia, desquamation, and necrosis of epithelial, epithelial lifting, oedema, lamellar fusion, collapsed secondary lamellae, curling of secondary lamellae and aneurism in the secondary lamellae were observed in gill tissues exposed to dichlorvos. Hepatic lesions in the liver tissues of fishes exposed to dichlorvos were characterized by cloudy swelling of hepatocytes, congestion, vacuolar degeneration, Karyolysis, karyohexis, dilation of sinusoids and nuclear hypertrophy.
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histopathology of lambda cyhalothrin on tissues gill kidney liver and intestine of cirrhinus mrigala
Environmental Toxicology and Pharmacology, 2007Co-Authors: Babu Velmurugan, Michael Selvanayagam, Elif Ipek Cengiz, Erhan ÜnlüAbstract:The histopathology of lambda-cyhalothrin, a synthetic pyrethroid pesticide, on the gill, liver, intestine and kidney tissues in fish, mrigal (Cirrhinus mrigala), which is one of Indian major carp species were determined by light microscopy. The fish were experimentally exposed to sub-lethal concentrations (0.3 ppb and 0.6 ppb) of lambda-cyhalothrin for 10 days. Tissues were normal in the control group. Epithelial hyperplasia, aneurism, epithelial necrosis, desquamation, epithelial lifting, oedema, shortening of secondary lamellae and lamellar fusion were observed in gill tissues exposed to lambda-cyhalothrin. Necrosis of tubular epithelium, cloudy swelling of epithelial cells of renal tubules, narrowing of the tubular lumen, contraction of the glomerulus and expansion of space inside the Bowman’s capsule were observed in the kidney tissues of fish after exposure. Hepatic lesions in fish exposed to lambda-cyhalothrin were characterized by hypertrophy of hepatocytes, cloudy degeneration, congestion, Karyolysis, karyohexis, dilatation of sinusoids and focal necrosis. The intestinal lesions included infiltration of eosinophils into the lamina propria and atrophy of epithelial cells. The present study proves its toxic potential in terms of the damages induced by lambda-cyhalothrin in organ level. In natural condition lambda-cyhalothrin will be less than the present study, but continuous usage of the pesticide might lead to the concentration that was used in the experimental condition. © 2007 Published by Elsevier B.V.
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histopathology of lambda cyhalothrin on tissues gill kidney liver and intestine of cirrhinus mrigala
Environmental Toxicology and Pharmacology, 2007Co-Authors: Babu Velmurugan, Michael Selvanayagam, Elif Ipek Cengiz, Erhan ÜnlüAbstract:The histopathology of lambda-cyhalothrin, a synthetic pyrethroid pesticide, on the gill, liver, intestine and kidney tissues in fish, mrigal (Cirrhinus mrigala), which is one of Indian major carp species were determined by light microscopy. The fish were experimentally exposed to sub-lethal concentrations (0.3 ppb and 0.6 ppb) of lambda-cyhalothrin for 10 days. Tissues were normal in the control group. Epithelial hyperplasia, aneurism, epithelial necrosis, desquamation, epithelial lifting, oedema, shortening of secondary lamellae and lamellar fusion were observed in gill tissues exposed to lambda-cyhalothrin. Necrosis of tubular epithelium, cloudy swelling of epithelial cells of renal tubules, narrowing of the tubular lumen, contraction of the glomerulus and expansion of space inside the Bowman’s capsule were observed in the kidney tissues of fish after exposure. Hepatic lesions in fish exposed to lambda-cyhalothrin were characterized by hypertrophy of hepatocytes, cloudy degeneration, congestion, Karyolysis, karyohexis, dilatation of sinusoids and focal necrosis. The intestinal lesions included infiltration of eosinophils into the lamina propria and atrophy of epithelial cells. The present study proves its toxic potential in terms of the damages induced by lambda-cyhalothrin in organ level. In natural condition lambda-cyhalothrin will be less than the present study, but continuous usage of the pesticide might lead to the concentration that was used in the experimental condition. © 2007 Published by Elsevier B.V.
