The Experts below are selected from a list of 291 Experts worldwide ranked by ideXlab platform
Salvatore De Maria - One of the best experts on this subject based on the ideXlab platform.
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A possible interplay between HR-HPV and stemness in tumor development: an in vivo investigation of CD133 as a putative marker of cancer stem Cell in HPV18-infected KB Cell Line.
APMIS : acta pathologica microbiologica et immunologica Scandinavica, 2020Co-Authors: Salvatore De Maria, Angela Santoro, Maria Pia Fuggetta, Romina Rocchetti, Andrea Cottarelli, Giulia Lanzilli, Paola Stiuso, Giuseppe Angelico, Saveria Spadola, Gian Franco ZannoniAbstract:High-risk HPVs (HR-HPVs) are DNA viruses considered as primary etiologic factors in malignancies of the low female genital tract. Their presence has also been documented in oropharyngeal and laryngeal cancers. However, HPV infection is considered a necessary but not sufficient cause of tumoral development; meantime, increasing evidences on the tumorigenic role of cancer stem Cells (CSCs) have been documented in the literature. CSCs represent a small subpopulation of neoplastic Cells with self-renewal potential, capable of maintaining tumor growth and Cell differentiation, also involved in metastatic process, recurrence, and resistance to chemotherapeutic agents. In the present study, performed on KB Cell Lines, we evaluated the tumor forming potential of CSCs, and their relationship with the HPV infection status. We started our study by identifying the most aggressive Cell Line on the minimal number of Cells being able of growth in vivo in a model of athymic nude mice (BALB/c nu/nu). We used an oral-derived KB Cell Line separated in the KB-CD133+ and KB-CD133- populations, by using immunomagnetic beads and fluorescence-activated Cell sorting (FACS). The separated populations were injected in athymic nude mice (BALB/c nu/nu). Xenograft tumors have been analyzed for tumor size, CD133 expression by immunohistochemistry (IHC) and for DNA HR-HPV integration by in situ hybridization (ISH), comparing CD133-enriched xenograft tumors versus the CD133 non-enriched ones. On standard conditions, the KB Cell Line has a poor population of glycosylated CD133 marker (
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a possible interplay between hr hpv and stemness in tumor development an in vivo investigation of cd133 as a putative marker of cancer stem Cell in hpv18 infected KB Cell Line
Apmis, 2020Co-Authors: Salvatore De Maria, Angela Santoro, Maria Pia Fuggetta, Romina Rocchetti, Andrea Cottarelli, Giulia Lanzilli, Paola Stiuso, Giuseppe Angelico, Saveria SpadolaAbstract:High-risk HPVs (HR-HPVs) are DNA viruses considered as primary etiologic factors in malignancies of the low female genital tract. Their presence has also been documented in oropharyngeal and laryngeal cancers. However, HPV infection is considered a necessary but not sufficient cause of tumoral development; meantime, increasing evidences on the tumorigenic role of cancer stem Cells (CSCs) have been documented in the literature. CSCs represent a small subpopulation of neoplastic Cells with self-renewal potential, capable of maintaining tumor growth and Cell differentiation, also involved in metastatic process, recurrence, and resistance to chemotherapeutic agents. In the present study, performed on KB Cell Lines, we evaluated the tumor forming potential of CSCs, and their relationship with the HPV infection status. We started our study by identifying the most aggressive Cell Line on the minimal number of Cells being able of growth in vivo in a model of athymic nude mice (BALB/c nu/nu). We used an oral-derived KB Cell Line separated in the KB-CD133+ and KB-CD133- populations, by using immunomagnetic beads and fluorescence-activated Cell sorting (FACS). The separated populations were injected in athymic nude mice (BALB/c nu/nu). Xenograft tumors have been analyzed for tumor size, CD133 expression by immunohistochemistry (IHC) and for DNA HR-HPV integration by in situ hybridization (ISH), comparing CD133-enriched xenograft tumors versus the CD133 non-enriched ones. On standard conditions, the KB Cell Line has a poor population of glycosylated CD133 marker (<5.0%) when investigated with antibodies versus CD133, and more specifically its glycosylated epitope (AC133). Enriched CD133 KB Cells possess a higher capacity of tumor growth in xenograft models of nude mice when compared to KB CD133-negative Cells. We observed that the AC133 epitope, extensively used to purifying hematopoietic stem Cells, is able to select an epithelial subpopulation of cancer stem Cells with aggressive behavior. We retain that CD133 may be a useful target in anticancer strategies including pharmacological and immunological therapies.
Liu Dong-juan - One of the best experts on this subject based on the ideXlab platform.
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Comparison of the ultrastructure of tumor Cell and tumor sphere Cell in human KB Cell Line and CCL227 Line
Journal of Modern Oncology, 2012Co-Authors: Liu Dong-juanAbstract:Objective:To compare the ultrastructure of tumor Cell and tumor sphere Cell in human KB Cell Line and CCL227 Cell Line.Methods: SSM was used to culture KB Cells and CCL227 Cells.The SFM was used to induce KB tumor sphere and CCL227 tumor sphere.The sections of transmission electron microscope of KB Cell,CCL227 Cell,KB tumor sphere Cell,CCL227 tumor sphere Cell were made by routine method,and were observed and photographed.Results: The Cells displayed two kinds of morphous roughly.One kind Cell had surface claw,more ribosome and more mitochondrion.Another Cell had not surface claw,less ribosome and mitochondrion,and more microfilaments appear around Cell nucleus.Chromatin in Cell nucleus keep to the side.Moreover,the amount of the latter was more in tumor sphere Cell than tumor Cell.Conclusion: The structure of tumor stem Cell has some changes with the change of the physiological functions.These change maybe result in a drop of protein synthesis.
Zheng Zhi-hong - One of the best experts on this subject based on the ideXlab platform.
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Expression of β-catenin in human KB Cell Line and tumor sphere Cell
Journal of Modern Oncology, 2011Co-Authors: Zheng Zhi-hongAbstract:Objectivf:To study the expression of signaling pathway β-catenin in human KB Cell Line and tumor sphere Cell.Methods:KB and KB tumor sphere Cells were cultured.RNA was extract respectively from KB Cell and KB tumor sphere Cell.The mRNA of β-catenin was detected by RT-PCR with primer designed by ourselves.The protein of β-catenin was detected by western.Resulsts:β-catenin of two kind Cell were positive,but the mRNA amount of KB Cell was higher than tumor sphere Cell.Western results showes two kind Cell were positive,but the protein amount of tumor sphere Cell was higher than KB Cell.Conclusion: β-catenin protein is accumulated and transcribed in KB Cell and KB tumor sphere Cell.
Saveria Spadola - One of the best experts on this subject based on the ideXlab platform.
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A possible interplay between HR-HPV and stemness in tumor development: an in vivo investigation of CD133 as a putative marker of cancer stem Cell in HPV18-infected KB Cell Line.
APMIS : acta pathologica microbiologica et immunologica Scandinavica, 2020Co-Authors: Salvatore De Maria, Angela Santoro, Maria Pia Fuggetta, Romina Rocchetti, Andrea Cottarelli, Giulia Lanzilli, Paola Stiuso, Giuseppe Angelico, Saveria Spadola, Gian Franco ZannoniAbstract:High-risk HPVs (HR-HPVs) are DNA viruses considered as primary etiologic factors in malignancies of the low female genital tract. Their presence has also been documented in oropharyngeal and laryngeal cancers. However, HPV infection is considered a necessary but not sufficient cause of tumoral development; meantime, increasing evidences on the tumorigenic role of cancer stem Cells (CSCs) have been documented in the literature. CSCs represent a small subpopulation of neoplastic Cells with self-renewal potential, capable of maintaining tumor growth and Cell differentiation, also involved in metastatic process, recurrence, and resistance to chemotherapeutic agents. In the present study, performed on KB Cell Lines, we evaluated the tumor forming potential of CSCs, and their relationship with the HPV infection status. We started our study by identifying the most aggressive Cell Line on the minimal number of Cells being able of growth in vivo in a model of athymic nude mice (BALB/c nu/nu). We used an oral-derived KB Cell Line separated in the KB-CD133+ and KB-CD133- populations, by using immunomagnetic beads and fluorescence-activated Cell sorting (FACS). The separated populations were injected in athymic nude mice (BALB/c nu/nu). Xenograft tumors have been analyzed for tumor size, CD133 expression by immunohistochemistry (IHC) and for DNA HR-HPV integration by in situ hybridization (ISH), comparing CD133-enriched xenograft tumors versus the CD133 non-enriched ones. On standard conditions, the KB Cell Line has a poor population of glycosylated CD133 marker (
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a possible interplay between hr hpv and stemness in tumor development an in vivo investigation of cd133 as a putative marker of cancer stem Cell in hpv18 infected KB Cell Line
Apmis, 2020Co-Authors: Salvatore De Maria, Angela Santoro, Maria Pia Fuggetta, Romina Rocchetti, Andrea Cottarelli, Giulia Lanzilli, Paola Stiuso, Giuseppe Angelico, Saveria SpadolaAbstract:High-risk HPVs (HR-HPVs) are DNA viruses considered as primary etiologic factors in malignancies of the low female genital tract. Their presence has also been documented in oropharyngeal and laryngeal cancers. However, HPV infection is considered a necessary but not sufficient cause of tumoral development; meantime, increasing evidences on the tumorigenic role of cancer stem Cells (CSCs) have been documented in the literature. CSCs represent a small subpopulation of neoplastic Cells with self-renewal potential, capable of maintaining tumor growth and Cell differentiation, also involved in metastatic process, recurrence, and resistance to chemotherapeutic agents. In the present study, performed on KB Cell Lines, we evaluated the tumor forming potential of CSCs, and their relationship with the HPV infection status. We started our study by identifying the most aggressive Cell Line on the minimal number of Cells being able of growth in vivo in a model of athymic nude mice (BALB/c nu/nu). We used an oral-derived KB Cell Line separated in the KB-CD133+ and KB-CD133- populations, by using immunomagnetic beads and fluorescence-activated Cell sorting (FACS). The separated populations were injected in athymic nude mice (BALB/c nu/nu). Xenograft tumors have been analyzed for tumor size, CD133 expression by immunohistochemistry (IHC) and for DNA HR-HPV integration by in situ hybridization (ISH), comparing CD133-enriched xenograft tumors versus the CD133 non-enriched ones. On standard conditions, the KB Cell Line has a poor population of glycosylated CD133 marker (<5.0%) when investigated with antibodies versus CD133, and more specifically its glycosylated epitope (AC133). Enriched CD133 KB Cells possess a higher capacity of tumor growth in xenograft models of nude mice when compared to KB CD133-negative Cells. We observed that the AC133 epitope, extensively used to purifying hematopoietic stem Cells, is able to select an epithelial subpopulation of cancer stem Cells with aggressive behavior. We retain that CD133 may be a useful target in anticancer strategies including pharmacological and immunological therapies.
Rei Han - One of the best experts on this subject based on the ideXlab platform.
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Symposium-in-Print Synthesis, Characterization and Photodynamic Activity of Amino- substituted Hypocrellin Derivatives ¶
2001Co-Authors: Yi-jin Tang, Hong-yan Liu, Rei HanAbstract:Three new hypocrellin derivatives, amino- or amino acid‐substituted on the side ring of hypocrellin B (HB), were synthesized by the reactions of HB with 3-methoxypropylamine, 6-aminohexanoic acid and g-amino-n-butyric acid, respectively. The structures of these compounds were characterized with proton nuclear magnetic resonance spectra, infrared spectra and mass spectra. The UV‐visible absorption spectra, singlet oxygen‐generating quantum yield and amphiphilicities of hypocrellin derivatives were measured and compared with HB, the parent compound. These derivatives showed strong absorption in the domain of the phototherapeutic window (600‐900 nm) and improved amphiphilicity. HB and the derivatives were preliminarily tested for their photodynamic effects on human oral cavity epithelial carcinoma KB Cell Line in vitro. Two amino acid‐substituted hypocrellins showed phototoxicity to the KB Cell Line. At an inhibitory dosage of 50% killing only 0.51 mmol L 21 com
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Synthesis, characterization and photodynamic activity of amino-substituted hypocrellin derivatives.
Photochemistry and photobiology, 2001Co-Authors: Yi-jin Tang, Hong-yan Liu, Rei HanAbstract:Abstract Three new hypocrellin derivatives, amino- or amino acid–substituted on the side ring of hypocrellin B (HB), were synthesized by the reactions of HB with 3-methoxypropylamine, 6-aminohexanoic acid and γ-amino-n-butyric acid, respectively. The structures of these compounds were characterized with proton nuclear magnetic resonance spectra, infrared spectra and mass spectra. The UV–visible absorption spectra, singlet oxygen–generating quantum yield and amphiphilicities of hypocrellin derivatives were measured and compared with HB, the parent compound. These derivatives showed strong absorption in the domain of the phototherapeutic window (600–900 nm) and improved amphiphilicity. HB and the derivatives were preliminarily tested for their photodynamic effects on human oral cavity epithelial carcinoma KB Cell Line in vitro. Two amino acid–substituted hypocrellins showed phototoxicity to the KB Cell Line. At an inhibitory dosage of 50% killing only 0.51 μmol L−1 compound 3 (or 0.88 μmol L−1 compound 2) a...
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Photodynamic action of hypocrellin dyes: structure–activity relationships
Dyes and Pigments, 1999Co-Authors: Hong-yan Liu, Rei Han, Li-jin JiangAbstract:Abstract Hypocrellin and its derivatives were compared for their photodynamic effects on human oral cavity epithelial carcinoma KB Cell Line. The amphiphilicity as well as the singlet oxygen generating quantum yield of the hypocrellin dyes affected their photodynamic activity. The most hydrophilic dyes exhibited the lowest phototoxic activity, whereas the hydrophobic and amphiphilic dyes with higher singlet oxygen-generating quantum yield, exhibited high photodynamic activity. Cysteamine mono- and di-substituted hypocrellin B and cysteine mono-substituted hypocrellin B, demonstrating strong red absorption in the domain of phototherapeutic window (600–900 nm), proper hydrophobic and amphiphilic properties and high photocytotoxicity to KB Cells, might prove to be potential phototherapeutic agents.