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Francesco Aragona - One of the best experts on this subject based on the ideXlab platform.
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Keratin 7 expression as an early marker of reflux related columnar mucosa without intestinal metaplasia in the esophagus
Medical Science Monitor, 2009Co-Authors: Daniela Cabibi, Eugenio Fiorentino, G Pantuso, Antonio Mastrosimone, Cosimo Callari, Matilde Cacciatore, Maria Campione, Francesco AragonaAbstract:Background: The role of Barrett esophagus in carcinogenesis is widely accepted, but the significance of esophageal columnar mucosa without histological intestinal metaplasia, known as columnar-lined esophagus, is debated. Material/Methods: We studied 128 patients free of Helicobacter pylori with reflux-related symptoms and columnar mucosa in the esophagus at endoscopy, 106 patients with Barrett esophagus (referred to as the Barrett group) and 22 patients without intestinal metaplasia (columnar group). Samples from 20 subjects free of H. pylori were used as controls. Immunostaining for Keratin 7 (KRT7), Keratin 20 (KRT20), caudal type homeobox 2 (CDX2), mucin 2, oligomeric mucus/gel-forming (MUC2), and tumor protein p53 (TP53) was assessed. Results: Samples taken 1 cm above the gastroesophageal junction showed KRT7 staining in all cases in the Barrett and columnar groups and none in the control group. Immunostaining for TP53 was absent in the control group, and more frequent in the columnar group (7, 31.8%) compared with the Barrett group (14, 13.2%, P=0.033). In the columnar group, low grade dysplasia and TP53 expression was seen in 7 of 22 biopsy specimens (31.8%) at baseline and in 4 additional specimens after 2 years, for a total of 11 specimens (50.0%). Conclusions: The expression of KRT7 might help to explain the pathological, reflux-related nature of columnar-lined esophagus, as aberrant expression in a very early stage of the multistep Barrett esophagus progression. Expression of KRT7 may occur in basal glandular cells as a result of their multi-potentiality and susceptibility to immunophenotype changes induced by reflux.
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Keratin 7 expression as an early marker of reflux related columnar mucosa without intestinal metaplasia in the esophagus
Medical Science Monitor, 2009Co-Authors: Daniela Cabibi, Eugenio Fiorentino, G Pantuso, Antonio Mastrosimone, Cosimo Callari, Matilde Cacciatore, Maria Campione, Francesco AragonaAbstract:BACKGROUND: The role of Barrett esophagus in carcinogenesis is widely accepted, but the significance of esophageal columnar mucosa without histological intestinal metaplasia, known as columnar-lined esophagus, is debated. MATERIAL/METHODS: We studied 128 patients free of Helicobacter pylori with reflux-related symptoms and columnar mucosa in the esophagus at endoscopy, 106 patients with Barrett esophagus (referred to as the Barrett group) and 22 patients without intestinal metaplasia (columnar group). Samples from 20 subjects free of H. pylori were used as controls. Immunostaining for Keratin 7 (KRT7), Keratin 20 (KRT20), caudal type homeobox 2 (CDX2), mucin 2, oligomeric mucus/gel-forming (MUC2), and tumor protein p53 (TP53) was assessed. RESULTS: Samples taken 1 cm above the gastroesophageal junction showed KRT7 staining in all cases in the Barrett and columnar groups and none in the control group. Immunostaining for TP53 was absent in the control group, and more frequent in the columnar group (7, 31.8%) compared with the Barrett group (14, 13.2%, P=0.033). In the columnar group, low grade dysplasia and TP53 expression was seen in 7 of 22 biopsy specimens (31.8%) at baseline and in 4 additional specimens after 2 years, for a total of 11 specimens (50.0%). CONCLUSIONS: The expression of KRT7 might help to explain the pathological, reflux-related nature of columnar-lined esophagus, as aberrant expression in a very early stage of the multistep Barrett esophagus progression. Expression of KRT7 may occur in basal glandular cells as a result of their multipotentiality and susceptibility to immunophenotype changes induced by reflux.
Bishr Omary - One of the best experts on this subject based on the ideXlab platform.
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Keratin 20 serine 13 phosphorylation is a stress and intestinal goblet cell marker
Journal of Biological Chemistry, 2006Co-Authors: Qin Zhou, Monique Cadrin, Harald Herrmann, Chehong Chen, Robert J Chalkley, Alma L Burlingame, Bishr OmaryAbstract:Keratin polypeptide 20 (K20) is an intermediate filament protein with preferential expression in epithelia of the stomach, intestine, uterus, and bladder and in Merkel cells of the skin. K20 expression is used as a marker to distinguish metastatic tumor origin, but nothing is known regarding its regulation and function. We studied K20 phosphorylation as a first step toward understanding its physiologic role. K20 phosphorylation occurs preferentially on serine, with a high stoichiometry as compared with Keratin polypeptides 18 and 19. Mass spectrometry analysis predicted that either K20 Ser13 or Ser14 was a likely phosphorylation site, and Ser13 was confirmed as the phospho-moiety using mutation and transfection analysis and generation of an anti-K20-phospho-Ser13 antibody. K20 Ser13 phosphorylation increases after protein kinase C activation, and Ser13-to-Ala mutation interferes with Keratin filament reorganization in transfected cells. In physiological contexts, K20 degradation and associated Ser13 hyperphosphorylation occur during apoptosis, and chemically induced mouse colitis also promotes Ser13 phosphorylation. Among mouse small intestinal enterocytes, K20 Ser13 is pref erentially phosphorylated in goblet cells and undergoes dramatic hyperphosphorylation after starvation and mucin secretion. Therefore, K20 Ser13 is a highly dynamic protein kinase C-related phosphorylation site that is induced during apoptosis and tissue injury. K20 Ser13 phosphorylation also serves as a unique marker of small intestinal goblet cells.
Frank Smedts - One of the best experts on this subject based on the ideXlab platform.
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Keratins 7 and 20 as diagnostic markers of carcinomas metastatic to the ovary
Human Pathology, 1995Co-Authors: Carla C.a.p. Wauters, Louis G.m Gerrits, Frank Smedts, Fred T. Bosman, Frans C. S. RamaekersAbstract:Abstract The most common carcinomas metastatic to the ovary that mimic ovarian primaries are colonic adenocarcinomas and endometrial carcinomas. Conventional histochemical staining procedures, even in combination with additional immunohistochemical assays, are of limited value in distinguishing between these metastases and primary ovarian carcinomas. In this study we investigated whether the application of monoclonal antibodies against Keratins 7, 8, and 20 could help in differentiating between these categories. The reactivity patterns of 40 carcinomas metastatic to the ovary were compared with those of their primary carcinomas on the one hand and with various primary ovarian carcinomas and mesotheliomas on the other. Colon cancer metastatic to the ovary was Keratin 7 negative and Keratin 20 positive in 94% of the cases; in contrast, all primary ovarian carcinomas were Keratin 7 positive and Keratin 20 negative, with the exception of two cases of mucinous cystadenocarcinoma. Ovarian metastases of gastric cancer usually contained Keratins 7 and 20. Metastases of endometrial cancer to the ovary and primary ovarian carcinomas usually showed similar Keratin expression. We propose that Keratin 7 and 20 antibodies may be of help to distinguish between primary ovarian carcinomas and carcinoma metastases in the ovary.
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expression of Keratins 1 6 15 16 and 20 in normal cervical epithelium squamous metaplasia cervical intraepithelial neoplasia and cervical carcinoma
American Journal of Pathology, 1993Co-Authors: Frank Smedts, F. C. S. Ramaekers, K Keijser, M Link, P VooijsAbstract:Expression of Keratins 1, 6, 15, 16, and 20 was examined in normal cervical epithelia, squamous metaplasia, various grades of cervical intraepithelial neoplasia, and both squamous cell carcinomas and adenocarcinomas of the cervix with monospecific antibodies. Ectocervical epithelium contains all of these Keratins except Keratin 20. They show a heterogeneous distribution, with a basally restricted detection of Keratin 15. Endocervical columnar cells were found to contain significant amounts of Keratin 16, whereas the subcolumnar reserve cells expressed considerable amounts of Keratin 15 and 16, and frequently Keratin 6. These reserve cell Keratins were also found in immature and mature squamous metaplastic epithelium. In the cervical intraepithelial neoplastic lesions they were generally found with increasing intensity as the severity of the lesion progressed. In the Keratinizing variety of squamous cell carcinoma of the cervix, these three Keratins seem to constitute an important part of the intermediate filament cytoskeleton, whereas in nonKeratinizing squamous cell carcinoma, they occur to a much lesser extent. Surprisingly, these Keratins were also occasionally found in adenocarcinomas. From these data we conclude that the Keratin phenotype of reserve cells and endocervical columnar cells is more complex than previously suggested. In particular, the Keratins occurring in reserve cells are also present in most of the premalignant and in a considerable number of the malignant lesions of the cervix. The differentiation features of the various carcinoma types are, however, reflected in their specific Keratin filament composition.
Daniela Cabibi - One of the best experts on this subject based on the ideXlab platform.
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Keratin 7 expression as an early marker of reflux related columnar mucosa without intestinal metaplasia in the esophagus
Medical Science Monitor, 2009Co-Authors: Daniela Cabibi, Eugenio Fiorentino, G Pantuso, Antonio Mastrosimone, Cosimo Callari, Matilde Cacciatore, Maria Campione, Francesco AragonaAbstract:Background: The role of Barrett esophagus in carcinogenesis is widely accepted, but the significance of esophageal columnar mucosa without histological intestinal metaplasia, known as columnar-lined esophagus, is debated. Material/Methods: We studied 128 patients free of Helicobacter pylori with reflux-related symptoms and columnar mucosa in the esophagus at endoscopy, 106 patients with Barrett esophagus (referred to as the Barrett group) and 22 patients without intestinal metaplasia (columnar group). Samples from 20 subjects free of H. pylori were used as controls. Immunostaining for Keratin 7 (KRT7), Keratin 20 (KRT20), caudal type homeobox 2 (CDX2), mucin 2, oligomeric mucus/gel-forming (MUC2), and tumor protein p53 (TP53) was assessed. Results: Samples taken 1 cm above the gastroesophageal junction showed KRT7 staining in all cases in the Barrett and columnar groups and none in the control group. Immunostaining for TP53 was absent in the control group, and more frequent in the columnar group (7, 31.8%) compared with the Barrett group (14, 13.2%, P=0.033). In the columnar group, low grade dysplasia and TP53 expression was seen in 7 of 22 biopsy specimens (31.8%) at baseline and in 4 additional specimens after 2 years, for a total of 11 specimens (50.0%). Conclusions: The expression of KRT7 might help to explain the pathological, reflux-related nature of columnar-lined esophagus, as aberrant expression in a very early stage of the multistep Barrett esophagus progression. Expression of KRT7 may occur in basal glandular cells as a result of their multi-potentiality and susceptibility to immunophenotype changes induced by reflux.
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Keratin 7 expression as an early marker of reflux related columnar mucosa without intestinal metaplasia in the esophagus
Medical Science Monitor, 2009Co-Authors: Daniela Cabibi, Eugenio Fiorentino, G Pantuso, Antonio Mastrosimone, Cosimo Callari, Matilde Cacciatore, Maria Campione, Francesco AragonaAbstract:BACKGROUND: The role of Barrett esophagus in carcinogenesis is widely accepted, but the significance of esophageal columnar mucosa without histological intestinal metaplasia, known as columnar-lined esophagus, is debated. MATERIAL/METHODS: We studied 128 patients free of Helicobacter pylori with reflux-related symptoms and columnar mucosa in the esophagus at endoscopy, 106 patients with Barrett esophagus (referred to as the Barrett group) and 22 patients without intestinal metaplasia (columnar group). Samples from 20 subjects free of H. pylori were used as controls. Immunostaining for Keratin 7 (KRT7), Keratin 20 (KRT20), caudal type homeobox 2 (CDX2), mucin 2, oligomeric mucus/gel-forming (MUC2), and tumor protein p53 (TP53) was assessed. RESULTS: Samples taken 1 cm above the gastroesophageal junction showed KRT7 staining in all cases in the Barrett and columnar groups and none in the control group. Immunostaining for TP53 was absent in the control group, and more frequent in the columnar group (7, 31.8%) compared with the Barrett group (14, 13.2%, P=0.033). In the columnar group, low grade dysplasia and TP53 expression was seen in 7 of 22 biopsy specimens (31.8%) at baseline and in 4 additional specimens after 2 years, for a total of 11 specimens (50.0%). CONCLUSIONS: The expression of KRT7 might help to explain the pathological, reflux-related nature of columnar-lined esophagus, as aberrant expression in a very early stage of the multistep Barrett esophagus progression. Expression of KRT7 may occur in basal glandular cells as a result of their multipotentiality and susceptibility to immunophenotype changes induced by reflux.
Frans C. S. Ramaekers - One of the best experts on this subject based on the ideXlab platform.
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Keratins 7 and 20 as diagnostic markers of carcinomas metastatic to the ovary
Human Pathology, 1995Co-Authors: Carla C.a.p. Wauters, Louis G.m Gerrits, Frank Smedts, Fred T. Bosman, Frans C. S. RamaekersAbstract:Abstract The most common carcinomas metastatic to the ovary that mimic ovarian primaries are colonic adenocarcinomas and endometrial carcinomas. Conventional histochemical staining procedures, even in combination with additional immunohistochemical assays, are of limited value in distinguishing between these metastases and primary ovarian carcinomas. In this study we investigated whether the application of monoclonal antibodies against Keratins 7, 8, and 20 could help in differentiating between these categories. The reactivity patterns of 40 carcinomas metastatic to the ovary were compared with those of their primary carcinomas on the one hand and with various primary ovarian carcinomas and mesotheliomas on the other. Colon cancer metastatic to the ovary was Keratin 7 negative and Keratin 20 positive in 94% of the cases; in contrast, all primary ovarian carcinomas were Keratin 7 positive and Keratin 20 negative, with the exception of two cases of mucinous cystadenocarcinoma. Ovarian metastases of gastric cancer usually contained Keratins 7 and 20. Metastases of endometrial cancer to the ovary and primary ovarian carcinomas usually showed similar Keratin expression. We propose that Keratin 7 and 20 antibodies may be of help to distinguish between primary ovarian carcinomas and carcinoma metastases in the ovary.
