The Experts below are selected from a list of 51 Experts worldwide ranked by ideXlab platform
Fei Zhang - One of the best experts on this subject based on the ideXlab platform.
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R (2013) Isolation of Irkut Virus from a Murina leucogaster bat
2016Co-Authors: Ye Liu, Shoufeng Zhang, Jinghui Zhao, Fei ZhangAbstract:Background and objectives: Bats are recognized as a major reservoir of lyssaViruses; however, no bat lyssaVirus has been isolated in Asia except for Aravan and Khujand Virus in Central Asia. All Chinese lyssaVirus isolates in previous reports have been of species rabies Virus, mainly from dogs. Following at least two recent bat-associated human rabies-like cases in northeast China, we have initiated a study of the prevalence of lyssaViruses in bats in Jilin province and their public health implications. A bat lyssaVirus has been isolated and its pathogenicity in mice and genomic alignment have been determined. Results: We report the first isolation of a bat lyssaVirus in China, from the brain of a northeastern bat, Murina leucogaster. Its nucleoprotein gene shared 92.4%/98.9 % (nucleotide) and 92.2%/98.8 % (amino acid) identity with the two known Irkut Virus isolates from Russia, and was designated IRKV-THChina12. Following intracranial and intramuscular injection, IRKV-THChina12 produced rabies-like symptoms in adult mice with a short inoculation period and high mortality. Nucleotide sequence analysis showed that IRKV-THChina12 has the same genomic organization as other lyssaViruses and its isolation provides an independent origin for the species IRKV. Conclusions: We have identified the existence of a bat lyssaVirus in a common Chinese bat species. Its high pathogenicity in adult mice suggests that public warnings and medical education regarding bat bites in China should be increased, and tha
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Isolation of Irkut Virus from a Murina leucogaster bat in China.
PLoS neglected tropical diseases, 2013Co-Authors: Ye Liu, Shoufeng Zhang, Jinghui Zhao, Fei ZhangAbstract:Background and objectives Bats are recognized as a major reservoir of lyssaViruses; however, no bat lyssaVirus has been isolated in Asia except for Aravan and Khujand Virus in Central Asia. All Chinese lyssaVirus isolates in previous reports have been of species rabies Virus, mainly from dogs. Following at least two recent bat-associated human rabies-like cases in northeast China, we have initiated a study of the prevalence of lyssaViruses in bats in Jilin province and their public health implications. A bat lyssaVirus has been isolated and its pathogenicity in mice and genomic alignment have been determined. Results We report the first isolation of a bat lyssaVirus in China, from the brain of a northeastern bat, Murina leucogaster. Its nucleoprotein gene shared 92.4%/98.9% (nucleotide) and 92.2%/98.8% (amino acid) identity with the two known Irkut Virus isolates from Russia, and was designated IRKV-THChina12. Following intracranial and intramuscular injection, IRKV-THChina12 produced rabies-like symptoms in adult mice with a short inoculation period and high mortality. Nucleotide sequence analysis showed that IRKV-THChina12 has the same genomic organization as other lyssaViruses and its isolation provides an independent origin for the species IRKV. Conclusions We have identified the existence of a bat lyssaVirus in a common Chinese bat species. Its high pathogenicity in adult mice suggests that public warnings and medical education regarding bat bites in China should be increased, and that surveillance be extended to provide a better understanding of Irkut Virus ecology and its significance for public health.
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Maximum likelihood of the lyssaVirus-rooted phylogenetic tree based on the complete N sequences.
2013Co-Authors: Ye Liu, Shoufeng Zhang, Jinghui Zhao, Fei ZhangAbstract:The isolate, IRKV-THChina12, is marked using black triangle. Bootstrap values are presented as percentages for key nodes on the tree, and branch lengths are indicated using a scale bar. EBLV-1: European bat lyssaVirus-1; DUVV: Duvenhage Virus; IRKV: Irkut Virus; ARAV: Aravan Virus; BBLV: Bokeloh bat lyssaVirus; KHUV: Khujand Virus; EBLV-2: European bat lyssaVirus-2; ABLV: Australian bat lyssaVirus; RABV: rabies Virus; WCBV: West Caucasian bat Virus; SHIBV: Shimoni bat Virus; MOKV: Mokola Virus; LBV: Lagos bat Virus; and IKOV: Ikoma lyssaVirus.
Charles E Rupprecht - One of the best experts on this subject based on the ideXlab platform.
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experimental infection of big brown bats eptesicus fuscus with eurasian bat lyssaViruses aravan Khujand and irkut Virus
Archives of Virology, 2006Co-Authors: Gareth J Hughes, Ivan V Kuzmin, A Schmitz, Jesse D Blanton, Jamie Manangan, Staci M Murphy, Charles E RupprechtAbstract:Here we describe the results of experimental infections of captive big brown bats (Eptesicus fuscus) with three newly isolated bat lyssaViruses from Eurasia (Aravan, Khujand, and Irkut Viruses). Infection of E. fuscus was moderate (total, 55–75%). There was no evidence of transmission to in-contact cage mates. Incubation periods for Irkut Virus infection were significantly shorter (p < 0.05) than for either Aravan or Khujand Virus infections. In turn, quantification of viral RNA by TaqMan PCR suggests that the dynamics of Irkut Virus infection may differ from those of Aravan/Khujand Virus infection. Although infectious Virus and viral RNA were detected in the brain of every rabid animal, dissemination to non-neuronal tissues was limited. Levels of viral RNA in brain of Aravan/Khujand Virus-infected bats was significantly correlated with the number of other tissues positive by TaqMan PCR (p < 0.05), whereas no such relationship was observed for Irkut Virus infection (where viral RNA was consistently detected in all tissues other than kidney). Infectious Virus was isolated sporadically from salivary glands, and both infectious Virus and viral RNA were obtained from oral swabs. The detection of viral RNA in oral swabs suggests that viral shedding in saliva occurred <5 days before the onset of clinical disease.
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Survey for Bat LyssaViruses, Thailand
Emerging infectious diseases, 2005Co-Authors: Boonlert Lumlertdacha, Ivan V Kuzmin, Kalyanee Boongird, Sawai Wanghongsa, Supaporn Wacharapluesadee, Lawan Chanhome, P. Khawplod, Thiravat Hemachudha, Charles E RupprechtAbstract:Surveillance for lyssaViruses was conducted among bat populations in 8 provinces in Thailand. In 2002 and 2003, a total of 932 bats of 11 species were captured and released after serum collection. LyssaVirus infection was determined by conducting Virus neutralization assays on bat serum samples. Of collected samples, 538 were either hemolysed or insufficient in volume, which left 394 suitable for analysis. These samples included the following: Pteropus lylei (n = 335), Eonycteris spelaea (n = 45), Hipposideros armiger (n = 13), and Rousettus leschennaulti (n = 1). No serum samples had evidence of neutralizing antibodies when tested against rabies Virus. However, 16 samples had detectable neutralizing antibodies against Aravan Virus, Khujand Virus, Irkut Virus, or Australian bat lyssaVirus; all were specifically associated with fruit bats P. lylei (n = 15) and E. spelaea (n = 1). These results are consistent with the presence of naturally occurring Viruses related to new putative lyssaVirus genotypes.
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Bat lyssaViruses (Aravan and Khujand) from Central Asia: phylogenetic relationships according to N, P and G gene sequences.
Virus research, 2003Co-Authors: Ivan V Kuzmin, Lillian A. Orciari, Yohko T. Arai, Jean S. Smith, Cathleen A. Hanlon, Yosuke Kameoka, Charles E RupprechtAbstract:Bat lyssaViruses Aravan and Khujand were isolated in southern Kyrgyzstan in 1991 and in northern Tajikistan in 2001, respectively. Preliminary studies with anti-nucleocapsid monoclonal antibodies suggested that the Viruses were distinct from other lyssaVirus serotypes. These data were supported by sequencing of the N gene of Aravan Virus. In the present study, we sequenced the entire N, P and G genes of both Aravan and Khujand Viruses and compared them with respective sequences of other lyssaViruses available from GenBank. The results suggested that each Virus should be considered as a newly recognized genotype according to the current approaches for genotype definition (amount of nucleotide identity of the N gene and bootstrap support of joining to certain phylogenetic groups). Use of different phylogenetic methods and comparison of different parts of the genomes generally suggested that Khujand Virus was mainly related to genotype 6, while Aravan Virus, on the one hand, was related to Khujand Virus, and, on the other hand, demonstrated moderate similarity to genotypes 4, 5 and 6. The potential significance of these new lyssaViruses for veterinary and public health should not be underestimated.
Ye Liu - One of the best experts on this subject based on the ideXlab platform.
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R (2013) Isolation of Irkut Virus from a Murina leucogaster bat
2016Co-Authors: Ye Liu, Shoufeng Zhang, Jinghui Zhao, Fei ZhangAbstract:Background and objectives: Bats are recognized as a major reservoir of lyssaViruses; however, no bat lyssaVirus has been isolated in Asia except for Aravan and Khujand Virus in Central Asia. All Chinese lyssaVirus isolates in previous reports have been of species rabies Virus, mainly from dogs. Following at least two recent bat-associated human rabies-like cases in northeast China, we have initiated a study of the prevalence of lyssaViruses in bats in Jilin province and their public health implications. A bat lyssaVirus has been isolated and its pathogenicity in mice and genomic alignment have been determined. Results: We report the first isolation of a bat lyssaVirus in China, from the brain of a northeastern bat, Murina leucogaster. Its nucleoprotein gene shared 92.4%/98.9 % (nucleotide) and 92.2%/98.8 % (amino acid) identity with the two known Irkut Virus isolates from Russia, and was designated IRKV-THChina12. Following intracranial and intramuscular injection, IRKV-THChina12 produced rabies-like symptoms in adult mice with a short inoculation period and high mortality. Nucleotide sequence analysis showed that IRKV-THChina12 has the same genomic organization as other lyssaViruses and its isolation provides an independent origin for the species IRKV. Conclusions: We have identified the existence of a bat lyssaVirus in a common Chinese bat species. Its high pathogenicity in adult mice suggests that public warnings and medical education regarding bat bites in China should be increased, and tha
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Isolation of Irkut Virus from a Murina leucogaster bat in China.
PLoS neglected tropical diseases, 2013Co-Authors: Ye Liu, Shoufeng Zhang, Jinghui Zhao, Fei ZhangAbstract:Background and objectives Bats are recognized as a major reservoir of lyssaViruses; however, no bat lyssaVirus has been isolated in Asia except for Aravan and Khujand Virus in Central Asia. All Chinese lyssaVirus isolates in previous reports have been of species rabies Virus, mainly from dogs. Following at least two recent bat-associated human rabies-like cases in northeast China, we have initiated a study of the prevalence of lyssaViruses in bats in Jilin province and their public health implications. A bat lyssaVirus has been isolated and its pathogenicity in mice and genomic alignment have been determined. Results We report the first isolation of a bat lyssaVirus in China, from the brain of a northeastern bat, Murina leucogaster. Its nucleoprotein gene shared 92.4%/98.9% (nucleotide) and 92.2%/98.8% (amino acid) identity with the two known Irkut Virus isolates from Russia, and was designated IRKV-THChina12. Following intracranial and intramuscular injection, IRKV-THChina12 produced rabies-like symptoms in adult mice with a short inoculation period and high mortality. Nucleotide sequence analysis showed that IRKV-THChina12 has the same genomic organization as other lyssaViruses and its isolation provides an independent origin for the species IRKV. Conclusions We have identified the existence of a bat lyssaVirus in a common Chinese bat species. Its high pathogenicity in adult mice suggests that public warnings and medical education regarding bat bites in China should be increased, and that surveillance be extended to provide a better understanding of Irkut Virus ecology and its significance for public health.
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Maximum likelihood of the lyssaVirus-rooted phylogenetic tree based on the complete N sequences.
2013Co-Authors: Ye Liu, Shoufeng Zhang, Jinghui Zhao, Fei ZhangAbstract:The isolate, IRKV-THChina12, is marked using black triangle. Bootstrap values are presented as percentages for key nodes on the tree, and branch lengths are indicated using a scale bar. EBLV-1: European bat lyssaVirus-1; DUVV: Duvenhage Virus; IRKV: Irkut Virus; ARAV: Aravan Virus; BBLV: Bokeloh bat lyssaVirus; KHUV: Khujand Virus; EBLV-2: European bat lyssaVirus-2; ABLV: Australian bat lyssaVirus; RABV: rabies Virus; WCBV: West Caucasian bat Virus; SHIBV: Shimoni bat Virus; MOKV: Mokola Virus; LBV: Lagos bat Virus; and IKOV: Ikoma lyssaVirus.
Shoufeng Zhang - One of the best experts on this subject based on the ideXlab platform.
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R (2013) Isolation of Irkut Virus from a Murina leucogaster bat
2016Co-Authors: Ye Liu, Shoufeng Zhang, Jinghui Zhao, Fei ZhangAbstract:Background and objectives: Bats are recognized as a major reservoir of lyssaViruses; however, no bat lyssaVirus has been isolated in Asia except for Aravan and Khujand Virus in Central Asia. All Chinese lyssaVirus isolates in previous reports have been of species rabies Virus, mainly from dogs. Following at least two recent bat-associated human rabies-like cases in northeast China, we have initiated a study of the prevalence of lyssaViruses in bats in Jilin province and their public health implications. A bat lyssaVirus has been isolated and its pathogenicity in mice and genomic alignment have been determined. Results: We report the first isolation of a bat lyssaVirus in China, from the brain of a northeastern bat, Murina leucogaster. Its nucleoprotein gene shared 92.4%/98.9 % (nucleotide) and 92.2%/98.8 % (amino acid) identity with the two known Irkut Virus isolates from Russia, and was designated IRKV-THChina12. Following intracranial and intramuscular injection, IRKV-THChina12 produced rabies-like symptoms in adult mice with a short inoculation period and high mortality. Nucleotide sequence analysis showed that IRKV-THChina12 has the same genomic organization as other lyssaViruses and its isolation provides an independent origin for the species IRKV. Conclusions: We have identified the existence of a bat lyssaVirus in a common Chinese bat species. Its high pathogenicity in adult mice suggests that public warnings and medical education regarding bat bites in China should be increased, and tha
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Isolation of Irkut Virus from a Murina leucogaster bat in China.
PLoS neglected tropical diseases, 2013Co-Authors: Ye Liu, Shoufeng Zhang, Jinghui Zhao, Fei ZhangAbstract:Background and objectives Bats are recognized as a major reservoir of lyssaViruses; however, no bat lyssaVirus has been isolated in Asia except for Aravan and Khujand Virus in Central Asia. All Chinese lyssaVirus isolates in previous reports have been of species rabies Virus, mainly from dogs. Following at least two recent bat-associated human rabies-like cases in northeast China, we have initiated a study of the prevalence of lyssaViruses in bats in Jilin province and their public health implications. A bat lyssaVirus has been isolated and its pathogenicity in mice and genomic alignment have been determined. Results We report the first isolation of a bat lyssaVirus in China, from the brain of a northeastern bat, Murina leucogaster. Its nucleoprotein gene shared 92.4%/98.9% (nucleotide) and 92.2%/98.8% (amino acid) identity with the two known Irkut Virus isolates from Russia, and was designated IRKV-THChina12. Following intracranial and intramuscular injection, IRKV-THChina12 produced rabies-like symptoms in adult mice with a short inoculation period and high mortality. Nucleotide sequence analysis showed that IRKV-THChina12 has the same genomic organization as other lyssaViruses and its isolation provides an independent origin for the species IRKV. Conclusions We have identified the existence of a bat lyssaVirus in a common Chinese bat species. Its high pathogenicity in adult mice suggests that public warnings and medical education regarding bat bites in China should be increased, and that surveillance be extended to provide a better understanding of Irkut Virus ecology and its significance for public health.
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Maximum likelihood of the lyssaVirus-rooted phylogenetic tree based on the complete N sequences.
2013Co-Authors: Ye Liu, Shoufeng Zhang, Jinghui Zhao, Fei ZhangAbstract:The isolate, IRKV-THChina12, is marked using black triangle. Bootstrap values are presented as percentages for key nodes on the tree, and branch lengths are indicated using a scale bar. EBLV-1: European bat lyssaVirus-1; DUVV: Duvenhage Virus; IRKV: Irkut Virus; ARAV: Aravan Virus; BBLV: Bokeloh bat lyssaVirus; KHUV: Khujand Virus; EBLV-2: European bat lyssaVirus-2; ABLV: Australian bat lyssaVirus; RABV: rabies Virus; WCBV: West Caucasian bat Virus; SHIBV: Shimoni bat Virus; MOKV: Mokola Virus; LBV: Lagos bat Virus; and IKOV: Ikoma lyssaVirus.
Jinghui Zhao - One of the best experts on this subject based on the ideXlab platform.
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R (2013) Isolation of Irkut Virus from a Murina leucogaster bat
2016Co-Authors: Ye Liu, Shoufeng Zhang, Jinghui Zhao, Fei ZhangAbstract:Background and objectives: Bats are recognized as a major reservoir of lyssaViruses; however, no bat lyssaVirus has been isolated in Asia except for Aravan and Khujand Virus in Central Asia. All Chinese lyssaVirus isolates in previous reports have been of species rabies Virus, mainly from dogs. Following at least two recent bat-associated human rabies-like cases in northeast China, we have initiated a study of the prevalence of lyssaViruses in bats in Jilin province and their public health implications. A bat lyssaVirus has been isolated and its pathogenicity in mice and genomic alignment have been determined. Results: We report the first isolation of a bat lyssaVirus in China, from the brain of a northeastern bat, Murina leucogaster. Its nucleoprotein gene shared 92.4%/98.9 % (nucleotide) and 92.2%/98.8 % (amino acid) identity with the two known Irkut Virus isolates from Russia, and was designated IRKV-THChina12. Following intracranial and intramuscular injection, IRKV-THChina12 produced rabies-like symptoms in adult mice with a short inoculation period and high mortality. Nucleotide sequence analysis showed that IRKV-THChina12 has the same genomic organization as other lyssaViruses and its isolation provides an independent origin for the species IRKV. Conclusions: We have identified the existence of a bat lyssaVirus in a common Chinese bat species. Its high pathogenicity in adult mice suggests that public warnings and medical education regarding bat bites in China should be increased, and tha
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Isolation of Irkut Virus from a Murina leucogaster bat in China.
PLoS neglected tropical diseases, 2013Co-Authors: Ye Liu, Shoufeng Zhang, Jinghui Zhao, Fei ZhangAbstract:Background and objectives Bats are recognized as a major reservoir of lyssaViruses; however, no bat lyssaVirus has been isolated in Asia except for Aravan and Khujand Virus in Central Asia. All Chinese lyssaVirus isolates in previous reports have been of species rabies Virus, mainly from dogs. Following at least two recent bat-associated human rabies-like cases in northeast China, we have initiated a study of the prevalence of lyssaViruses in bats in Jilin province and their public health implications. A bat lyssaVirus has been isolated and its pathogenicity in mice and genomic alignment have been determined. Results We report the first isolation of a bat lyssaVirus in China, from the brain of a northeastern bat, Murina leucogaster. Its nucleoprotein gene shared 92.4%/98.9% (nucleotide) and 92.2%/98.8% (amino acid) identity with the two known Irkut Virus isolates from Russia, and was designated IRKV-THChina12. Following intracranial and intramuscular injection, IRKV-THChina12 produced rabies-like symptoms in adult mice with a short inoculation period and high mortality. Nucleotide sequence analysis showed that IRKV-THChina12 has the same genomic organization as other lyssaViruses and its isolation provides an independent origin for the species IRKV. Conclusions We have identified the existence of a bat lyssaVirus in a common Chinese bat species. Its high pathogenicity in adult mice suggests that public warnings and medical education regarding bat bites in China should be increased, and that surveillance be extended to provide a better understanding of Irkut Virus ecology and its significance for public health.
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Maximum likelihood of the lyssaVirus-rooted phylogenetic tree based on the complete N sequences.
2013Co-Authors: Ye Liu, Shoufeng Zhang, Jinghui Zhao, Fei ZhangAbstract:The isolate, IRKV-THChina12, is marked using black triangle. Bootstrap values are presented as percentages for key nodes on the tree, and branch lengths are indicated using a scale bar. EBLV-1: European bat lyssaVirus-1; DUVV: Duvenhage Virus; IRKV: Irkut Virus; ARAV: Aravan Virus; BBLV: Bokeloh bat lyssaVirus; KHUV: Khujand Virus; EBLV-2: European bat lyssaVirus-2; ABLV: Australian bat lyssaVirus; RABV: rabies Virus; WCBV: West Caucasian bat Virus; SHIBV: Shimoni bat Virus; MOKV: Mokola Virus; LBV: Lagos bat Virus; and IKOV: Ikoma lyssaVirus.
