The Experts below are selected from a list of 300 Experts worldwide ranked by ideXlab platform
Elpidio Cesar B Nadala - One of the best experts on this subject based on the ideXlab platform.
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production of high efficiency of plating hepatitis a virus in primary african green monkey Kidney Cells
Experimental Biology and Medicine, 1992Co-Authors: Elpidio Cesar B NadalaAbstract:AbstractHigh-titered hepatitis A virus, strain HM-175, was produced in primary African green monkey Kidney Cells (5.5 × 1010 tissue culture ID50/850 cm2 roller bottle). The virus preparation had an efficiency of plating of 15 particles per infectious unit. Single-cycle growth kinetics of the adapted virus indicated that after an eclipse period of 2 days, maximal yields were attained 6 days after infection. [P.S.E.B.M. 1992, Vol 199]
Gerd Sutter - One of the best experts on this subject based on the ideXlab platform.
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Highly attenuated modified vaccinia virus Ankara replicates in baby hamster Kidney Cells, a potential host for virus propagation, but not in various human transformed and primary Cells
Journal of General Virology, 1998Co-Authors: Ingo Drexler, Karl Heller, Volker Erfle, Britta Wahren, Gerd SutterAbstract:Although desirable for safety reasons, the host range restrictions of modified vaccinia virus Ankara (MVA) make it less applicable for general use. Propagation in primary chicken embryo fibroblasts (CEF) requires particular cell culture experience and has no pre-established record of tissue culture reproducibility. We investigated a variety of established cell lines for productive virus growth and recombinant gene expression. Baby hamster Kidney Cells (BHK), a well-characterized, easily maintained cell line, supported MVA growth and as proficient expression of the E. coli lacZ reporter gene as the highly efficient CEF, whereas other cell lines were non-permissive or allowed only very limited MVA replication. Importantly, no virus production occurred in patient-derived infected primary human Cells. These results emphasize the safety and now improved accessibility of MVA for the development of expression vectors and live recombinant vaccines.
C Brouwer - One of the best experts on this subject based on the ideXlab platform.
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thiopurine metabolism and identification of the thiopurine metabolites transported by mrp4 and mrp5 overexpressed in human embryonic Kidney Cells
Molecular Pharmacology, 2002Co-Authors: Peter R Wielinga, John D Schuetz, Glen Reid, Liesbeth Van Deemter, E E Challa, I M Van Der Heijden, M De Haas, Annemieke Kuil, E Groeneveld, C BrouwerAbstract:Mercaptopurines have been used as anticancer agents for more than 40 years, and most acute lymphoblastic leukemias are treated with 6-mercaptopurine (6MP) or 6-thioguanine (TG). Overexpression of the two related multidrug resistance proteins MRP4 and MRP5 has been shown to confer some resistance against mercaptopurines, which has been attributed to extrusion of mercaptopurine metabolites by these transporters. We have analyzed the mercaptopurine metabolites formed in human embryonic Kidney Cells and determined which metabolites are extruded by MRP4 and MRP5. Incubation with 6MP led to the formation of thioinosine and thioxanthosine metabolites and we found that thio-IMP was transported by both MRP4 and MRP5; MRP5 showed the highest transport rate. In contrast, only MRP5 transported thioxanthosine monophosphate (tXMP). During incubation with TG, the monophosphorylated form of thioguanosine was transported by both MRP4 and MRP5; the highest transport rate was for MRP4. Similarly, only 6-methyl-thio-IMP was formed during incubation with 6-methyl mercaptopurine riboside. This compound was a substrate for both MRP4 and MRP5; MRP4 showed the highest transport rate. Our results show that all major thiopurine monophosphates important in the efficacy of mercaptopurine treatment are transported by MRP4 and MRP5, although the substrate specificity of the two transporters differs in detail.
Shuvo Roy - One of the best experts on this subject based on the ideXlab platform.
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apical shear stress enhanced organic cation transport in human oct2 mate1 transfected madin darby canine Kidney Cells involves ciliary sensing
Journal of Pharmacology and Experimental Therapeutics, 2019Co-Authors: Aishwarya Jayagopal, Paul Brakeman, Peter Soler, Nicholas Ferrell, William H Fissell, Deanna L Kroetz, Shuvo RoyAbstract:Active transport by renal proximal tubules plays a significant role in drug disposition. During drug development, estimates of renal excretion are essential to dose determination. Kidney bioreactors that reproduce physiologic cues in the Kidney, such as flow-induced shear stress, may better predict in vivo drug behavior than do current in vitro models. In this study, we investigated the role of shear stress on active transport of 4-(4-(dimethylamino)styryl)-N-methylpyridinium iodide (ASP+) by Madin-Darby canine Kidney Cells exogenously expressing the human organic cation transporters organic cation transporter 2 (OCT2) and multidrug and toxin extrusion protein 1 (MATE1). Cells cultured in a parallel plate under continuous media perfusion formed a tight monolayer with a high barrier to inulin. In response to increasing levels of shear stress (0.2-2 dynes/cm2), Cells showed a corresponding increase in transport of ASP+, reaching a maximal 4.2-fold increase at 2 dynes/cm2 compared with Cells cultured under static conditions. This transport was inhibited with imipramine, indicating active transport was present under shear stress conditions. Cells exposed to shear stress of 2 dynes/cm2 also showed an increase in RNA expression of both transfected human and endogenous OCT2 (3.7- and 2.0-fold, respectively). Removal of cilia by ammonium sulfate eliminated the effects of shear on ASP+ transport at 0.5 dynes/cm2 with no effect on ASP+ transport under static conditions. These results indicate that shear stress affects active transport of organic cations in renal tubular epithelial Cells in a cilia-dependent manner.
Suzanne U Emerson - One of the best experts on this subject based on the ideXlab platform.
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hepatitis e virus genotype 1 infection of swine Kidney Cells in vitro is inhibited at multiple levels
Journal of Virology, 2014Co-Authors: Hanh Nguyen, Priyanka Shukla, Udana Torian, Kristina Faulk, Suzanne U EmersonAbstract:Genotype 1 hepatitis E viruses (HEVs) are restricted to primate hosts, whereas genotype 3 HEVs predominantly infect swine, in addition to primates. In order to identify possible determinants of the host range, infectious recombinant viruses and chimeras of a genotype 1 isolate and a genotype 3 isolate were compared for their ability to infect versus transfect cultured human HepG2/C3A Cells and swine LLC-PK Cells. The patterns of luciferase expression from virus replicons containing the Gaussia luciferase gene in place of the viral ORF2 or ORF3 genes demonstrated that translation of the ORF2 capsid gene of genotype 1 virus is severely inhibited in swine Kidney Cells compared to its translation in rhesus macaque Kidney or human liver Cells. Therefore, this virus may produce insufficient capsid protein for optimal assembly in swine Cells. Infectivity assays with a virus containing a chimeric capsid protein confirmed that amino acids 456 to 605 of the virus capsid protein comprised the virus receptor-binding region and suggested that genotype 1 viruses may be prevented from infecting swine because genotype 1 viruses are unable to enter swine Cells. Rhesus macaque Cells appeared to be better than human Cells for growing the genotype 1 virus. These cell and virus combinations may serve as a useful in vitro model with which to study determinants of the natural host range of this virus.
