The Experts below are selected from a list of 126 Experts worldwide ranked by ideXlab platform
Masaru Tomozawa - One of the best experts on this subject based on the ideXlab platform.
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Preparation and characterization of S-[2-carboxy-1-1H-imidazol-4-yl)ethyl]glutathione and its derivatives as proposed precursors of S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteine, a compound found in human urine
Biochimica et Biophysica Acta, 1993Co-Authors: Masahiro Kinuta, Toshihiko Ubuka, Satsuki Yamada, Keishi Yukihiro, Masaru TomozawaAbstract:Abstract Formation of 3-[(carboxymethyl)thio]-3-(1H-imidazol-4-yl)propanoic acid (I) and S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteine II, compounds found in human urinee, has been demonstrated by enzymatic degradation of S-[2-carboxy-1(1H-imidazol-4-yl)ethyl]glutathione (III). Compound (III) was chemically synthetisized in 72% yield by incubating the reaction mixture of trans-urocanic acid and 3-fold excess GSH at 65°C for 1 wk, which was accompanied by formation of N-{S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteinyl}glycine (IV) in 15% yield. S-[2-Carboxy-1-(1H-imidazol-4-yl)ethyl]-N-γ-glutamylcysteine (V) was produced by partial hydrolysis of compound (III) in HCl. The synthesisized compounds were characterized mainly by fast-atom bombardment mass spectrometry and high-voltage paper electrophoresis as well as chemical degradation. Incubation of compound (III) with rat Kidney Homogenate in a Tris buffer (pH 8), formed compound (II) in 80% yield possibly via compound (IV). Yield of compound (II) was increased by adding glycylglycine to the reaction mixture. However, little degradation of compound (III) occurred in the use of rat liver, brain,heart, or spleen Homogenate as the enzyme source. Compound (II) was further metabolized to compound (I) by incubation with rat Kidney Homogenate in a phosphate buffer of pH 7.4 From these results, we suggest that the urinary compounds are products of enzymatic degradation of compound (III) and that GSH may participate in the metabolism of urocanic acid, the first catabolite of L -histidine.
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Preparation and characterization of S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]glutathione and its derivatives as proposed precursors of S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteine, a compound found in human urine.
Biochimica et biophysica acta, 1993Co-Authors: Masahiro Kinuta, Toshihiko Ubuka, Satsuki Yamada, Keishi Yukihiro, Masaru TomozawaAbstract:Formation of 3-[(carboxymethyl)thio]-3-(1H-imidazol-4-yl)propanoic acid (I) and S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteine (II), compounds found in human urine, has been demonstrated by enzymatic degradation of S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]glutathione (III). Compound (III) was chemically synthesized in 72% yield by incubating the reaction mixture of trans-urocanic acid and 3-fold excess GSH at 65 degrees C for 1 wk, which was accompanied by formation of N-(S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteinyl)glycine (IV) in 15% yield. S-[2-Carboxy-1-(1H-imidazol-4-yl)ethyl]-N-gamma-glutamylcysteine (V) was produced by partial hydrolysis of compound (III) in HCl. The synthesized compounds were characterized mainly by fast-atom bombardment mass spectrometry and high-voltage paper electrophoresis as well as chemical degradation. Incubation of compound (III) with rat Kidney Homogenate in a Tris buffer (pH 8), formed compound (II) in 80% yield possibly via compound (IV). Yield of compound (II) was increased by adding glycylglycine to the reaction mixture. However, little degradation of compound (III) occurred in the use of rat liver, brain, heart or spleen Homogenate as the enzyme source. Compound (II) was further metabolized to compound (I) by incubation with rat Kidney Homogenate in a phosphate buffer of pH 7.4. From these results, we suggest that the urinary compounds are products of enzymatic degradation of compound (III) and that GSH may participate in the metabolism of urocanic acid, the first catabolite of L-histidine.
Masahiro Kinuta - One of the best experts on this subject based on the ideXlab platform.
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Preparation and characterization of S-[2-carboxy-1-1H-imidazol-4-yl)ethyl]glutathione and its derivatives as proposed precursors of S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteine, a compound found in human urine
Biochimica et Biophysica Acta, 1993Co-Authors: Masahiro Kinuta, Toshihiko Ubuka, Satsuki Yamada, Keishi Yukihiro, Masaru TomozawaAbstract:Abstract Formation of 3-[(carboxymethyl)thio]-3-(1H-imidazol-4-yl)propanoic acid (I) and S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteine II, compounds found in human urinee, has been demonstrated by enzymatic degradation of S-[2-carboxy-1(1H-imidazol-4-yl)ethyl]glutathione (III). Compound (III) was chemically synthetisized in 72% yield by incubating the reaction mixture of trans-urocanic acid and 3-fold excess GSH at 65°C for 1 wk, which was accompanied by formation of N-{S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteinyl}glycine (IV) in 15% yield. S-[2-Carboxy-1-(1H-imidazol-4-yl)ethyl]-N-γ-glutamylcysteine (V) was produced by partial hydrolysis of compound (III) in HCl. The synthesisized compounds were characterized mainly by fast-atom bombardment mass spectrometry and high-voltage paper electrophoresis as well as chemical degradation. Incubation of compound (III) with rat Kidney Homogenate in a Tris buffer (pH 8), formed compound (II) in 80% yield possibly via compound (IV). Yield of compound (II) was increased by adding glycylglycine to the reaction mixture. However, little degradation of compound (III) occurred in the use of rat liver, brain,heart, or spleen Homogenate as the enzyme source. Compound (II) was further metabolized to compound (I) by incubation with rat Kidney Homogenate in a phosphate buffer of pH 7.4 From these results, we suggest that the urinary compounds are products of enzymatic degradation of compound (III) and that GSH may participate in the metabolism of urocanic acid, the first catabolite of L -histidine.
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Preparation and characterization of S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]glutathione and its derivatives as proposed precursors of S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteine, a compound found in human urine.
Biochimica et biophysica acta, 1993Co-Authors: Masahiro Kinuta, Toshihiko Ubuka, Satsuki Yamada, Keishi Yukihiro, Masaru TomozawaAbstract:Formation of 3-[(carboxymethyl)thio]-3-(1H-imidazol-4-yl)propanoic acid (I) and S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteine (II), compounds found in human urine, has been demonstrated by enzymatic degradation of S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]glutathione (III). Compound (III) was chemically synthesized in 72% yield by incubating the reaction mixture of trans-urocanic acid and 3-fold excess GSH at 65 degrees C for 1 wk, which was accompanied by formation of N-(S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteinyl)glycine (IV) in 15% yield. S-[2-Carboxy-1-(1H-imidazol-4-yl)ethyl]-N-gamma-glutamylcysteine (V) was produced by partial hydrolysis of compound (III) in HCl. The synthesized compounds were characterized mainly by fast-atom bombardment mass spectrometry and high-voltage paper electrophoresis as well as chemical degradation. Incubation of compound (III) with rat Kidney Homogenate in a Tris buffer (pH 8), formed compound (II) in 80% yield possibly via compound (IV). Yield of compound (II) was increased by adding glycylglycine to the reaction mixture. However, little degradation of compound (III) occurred in the use of rat liver, brain, heart or spleen Homogenate as the enzyme source. Compound (II) was further metabolized to compound (I) by incubation with rat Kidney Homogenate in a phosphate buffer of pH 7.4. From these results, we suggest that the urinary compounds are products of enzymatic degradation of compound (III) and that GSH may participate in the metabolism of urocanic acid, the first catabolite of L-histidine.
Toshihiko Ubuka - One of the best experts on this subject based on the ideXlab platform.
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Preparation and characterization of S-[2-carboxy-1-1H-imidazol-4-yl)ethyl]glutathione and its derivatives as proposed precursors of S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteine, a compound found in human urine
Biochimica et Biophysica Acta, 1993Co-Authors: Masahiro Kinuta, Toshihiko Ubuka, Satsuki Yamada, Keishi Yukihiro, Masaru TomozawaAbstract:Abstract Formation of 3-[(carboxymethyl)thio]-3-(1H-imidazol-4-yl)propanoic acid (I) and S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteine II, compounds found in human urinee, has been demonstrated by enzymatic degradation of S-[2-carboxy-1(1H-imidazol-4-yl)ethyl]glutathione (III). Compound (III) was chemically synthetisized in 72% yield by incubating the reaction mixture of trans-urocanic acid and 3-fold excess GSH at 65°C for 1 wk, which was accompanied by formation of N-{S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteinyl}glycine (IV) in 15% yield. S-[2-Carboxy-1-(1H-imidazol-4-yl)ethyl]-N-γ-glutamylcysteine (V) was produced by partial hydrolysis of compound (III) in HCl. The synthesisized compounds were characterized mainly by fast-atom bombardment mass spectrometry and high-voltage paper electrophoresis as well as chemical degradation. Incubation of compound (III) with rat Kidney Homogenate in a Tris buffer (pH 8), formed compound (II) in 80% yield possibly via compound (IV). Yield of compound (II) was increased by adding glycylglycine to the reaction mixture. However, little degradation of compound (III) occurred in the use of rat liver, brain,heart, or spleen Homogenate as the enzyme source. Compound (II) was further metabolized to compound (I) by incubation with rat Kidney Homogenate in a phosphate buffer of pH 7.4 From these results, we suggest that the urinary compounds are products of enzymatic degradation of compound (III) and that GSH may participate in the metabolism of urocanic acid, the first catabolite of L -histidine.
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Preparation and characterization of S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]glutathione and its derivatives as proposed precursors of S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteine, a compound found in human urine.
Biochimica et biophysica acta, 1993Co-Authors: Masahiro Kinuta, Toshihiko Ubuka, Satsuki Yamada, Keishi Yukihiro, Masaru TomozawaAbstract:Formation of 3-[(carboxymethyl)thio]-3-(1H-imidazol-4-yl)propanoic acid (I) and S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteine (II), compounds found in human urine, has been demonstrated by enzymatic degradation of S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]glutathione (III). Compound (III) was chemically synthesized in 72% yield by incubating the reaction mixture of trans-urocanic acid and 3-fold excess GSH at 65 degrees C for 1 wk, which was accompanied by formation of N-(S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteinyl)glycine (IV) in 15% yield. S-[2-Carboxy-1-(1H-imidazol-4-yl)ethyl]-N-gamma-glutamylcysteine (V) was produced by partial hydrolysis of compound (III) in HCl. The synthesized compounds were characterized mainly by fast-atom bombardment mass spectrometry and high-voltage paper electrophoresis as well as chemical degradation. Incubation of compound (III) with rat Kidney Homogenate in a Tris buffer (pH 8), formed compound (II) in 80% yield possibly via compound (IV). Yield of compound (II) was increased by adding glycylglycine to the reaction mixture. However, little degradation of compound (III) occurred in the use of rat liver, brain, heart or spleen Homogenate as the enzyme source. Compound (II) was further metabolized to compound (I) by incubation with rat Kidney Homogenate in a phosphate buffer of pH 7.4. From these results, we suggest that the urinary compounds are products of enzymatic degradation of compound (III) and that GSH may participate in the metabolism of urocanic acid, the first catabolite of L-histidine.
Keishi Yukihiro - One of the best experts on this subject based on the ideXlab platform.
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Preparation and characterization of S-[2-carboxy-1-1H-imidazol-4-yl)ethyl]glutathione and its derivatives as proposed precursors of S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteine, a compound found in human urine
Biochimica et Biophysica Acta, 1993Co-Authors: Masahiro Kinuta, Toshihiko Ubuka, Satsuki Yamada, Keishi Yukihiro, Masaru TomozawaAbstract:Abstract Formation of 3-[(carboxymethyl)thio]-3-(1H-imidazol-4-yl)propanoic acid (I) and S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteine II, compounds found in human urinee, has been demonstrated by enzymatic degradation of S-[2-carboxy-1(1H-imidazol-4-yl)ethyl]glutathione (III). Compound (III) was chemically synthetisized in 72% yield by incubating the reaction mixture of trans-urocanic acid and 3-fold excess GSH at 65°C for 1 wk, which was accompanied by formation of N-{S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteinyl}glycine (IV) in 15% yield. S-[2-Carboxy-1-(1H-imidazol-4-yl)ethyl]-N-γ-glutamylcysteine (V) was produced by partial hydrolysis of compound (III) in HCl. The synthesisized compounds were characterized mainly by fast-atom bombardment mass spectrometry and high-voltage paper electrophoresis as well as chemical degradation. Incubation of compound (III) with rat Kidney Homogenate in a Tris buffer (pH 8), formed compound (II) in 80% yield possibly via compound (IV). Yield of compound (II) was increased by adding glycylglycine to the reaction mixture. However, little degradation of compound (III) occurred in the use of rat liver, brain,heart, or spleen Homogenate as the enzyme source. Compound (II) was further metabolized to compound (I) by incubation with rat Kidney Homogenate in a phosphate buffer of pH 7.4 From these results, we suggest that the urinary compounds are products of enzymatic degradation of compound (III) and that GSH may participate in the metabolism of urocanic acid, the first catabolite of L -histidine.
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Preparation and characterization of S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]glutathione and its derivatives as proposed precursors of S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteine, a compound found in human urine.
Biochimica et biophysica acta, 1993Co-Authors: Masahiro Kinuta, Toshihiko Ubuka, Satsuki Yamada, Keishi Yukihiro, Masaru TomozawaAbstract:Formation of 3-[(carboxymethyl)thio]-3-(1H-imidazol-4-yl)propanoic acid (I) and S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteine (II), compounds found in human urine, has been demonstrated by enzymatic degradation of S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]glutathione (III). Compound (III) was chemically synthesized in 72% yield by incubating the reaction mixture of trans-urocanic acid and 3-fold excess GSH at 65 degrees C for 1 wk, which was accompanied by formation of N-(S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteinyl)glycine (IV) in 15% yield. S-[2-Carboxy-1-(1H-imidazol-4-yl)ethyl]-N-gamma-glutamylcysteine (V) was produced by partial hydrolysis of compound (III) in HCl. The synthesized compounds were characterized mainly by fast-atom bombardment mass spectrometry and high-voltage paper electrophoresis as well as chemical degradation. Incubation of compound (III) with rat Kidney Homogenate in a Tris buffer (pH 8), formed compound (II) in 80% yield possibly via compound (IV). Yield of compound (II) was increased by adding glycylglycine to the reaction mixture. However, little degradation of compound (III) occurred in the use of rat liver, brain, heart or spleen Homogenate as the enzyme source. Compound (II) was further metabolized to compound (I) by incubation with rat Kidney Homogenate in a phosphate buffer of pH 7.4. From these results, we suggest that the urinary compounds are products of enzymatic degradation of compound (III) and that GSH may participate in the metabolism of urocanic acid, the first catabolite of L-histidine.
Satsuki Yamada - One of the best experts on this subject based on the ideXlab platform.
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Preparation and characterization of S-[2-carboxy-1-1H-imidazol-4-yl)ethyl]glutathione and its derivatives as proposed precursors of S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteine, a compound found in human urine
Biochimica et Biophysica Acta, 1993Co-Authors: Masahiro Kinuta, Toshihiko Ubuka, Satsuki Yamada, Keishi Yukihiro, Masaru TomozawaAbstract:Abstract Formation of 3-[(carboxymethyl)thio]-3-(1H-imidazol-4-yl)propanoic acid (I) and S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteine II, compounds found in human urinee, has been demonstrated by enzymatic degradation of S-[2-carboxy-1(1H-imidazol-4-yl)ethyl]glutathione (III). Compound (III) was chemically synthetisized in 72% yield by incubating the reaction mixture of trans-urocanic acid and 3-fold excess GSH at 65°C for 1 wk, which was accompanied by formation of N-{S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteinyl}glycine (IV) in 15% yield. S-[2-Carboxy-1-(1H-imidazol-4-yl)ethyl]-N-γ-glutamylcysteine (V) was produced by partial hydrolysis of compound (III) in HCl. The synthesisized compounds were characterized mainly by fast-atom bombardment mass spectrometry and high-voltage paper electrophoresis as well as chemical degradation. Incubation of compound (III) with rat Kidney Homogenate in a Tris buffer (pH 8), formed compound (II) in 80% yield possibly via compound (IV). Yield of compound (II) was increased by adding glycylglycine to the reaction mixture. However, little degradation of compound (III) occurred in the use of rat liver, brain,heart, or spleen Homogenate as the enzyme source. Compound (II) was further metabolized to compound (I) by incubation with rat Kidney Homogenate in a phosphate buffer of pH 7.4 From these results, we suggest that the urinary compounds are products of enzymatic degradation of compound (III) and that GSH may participate in the metabolism of urocanic acid, the first catabolite of L -histidine.
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Preparation and characterization of S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]glutathione and its derivatives as proposed precursors of S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteine, a compound found in human urine.
Biochimica et biophysica acta, 1993Co-Authors: Masahiro Kinuta, Toshihiko Ubuka, Satsuki Yamada, Keishi Yukihiro, Masaru TomozawaAbstract:Formation of 3-[(carboxymethyl)thio]-3-(1H-imidazol-4-yl)propanoic acid (I) and S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteine (II), compounds found in human urine, has been demonstrated by enzymatic degradation of S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]glutathione (III). Compound (III) was chemically synthesized in 72% yield by incubating the reaction mixture of trans-urocanic acid and 3-fold excess GSH at 65 degrees C for 1 wk, which was accompanied by formation of N-(S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]cysteinyl)glycine (IV) in 15% yield. S-[2-Carboxy-1-(1H-imidazol-4-yl)ethyl]-N-gamma-glutamylcysteine (V) was produced by partial hydrolysis of compound (III) in HCl. The synthesized compounds were characterized mainly by fast-atom bombardment mass spectrometry and high-voltage paper electrophoresis as well as chemical degradation. Incubation of compound (III) with rat Kidney Homogenate in a Tris buffer (pH 8), formed compound (II) in 80% yield possibly via compound (IV). Yield of compound (II) was increased by adding glycylglycine to the reaction mixture. However, little degradation of compound (III) occurred in the use of rat liver, brain, heart or spleen Homogenate as the enzyme source. Compound (II) was further metabolized to compound (I) by incubation with rat Kidney Homogenate in a phosphate buffer of pH 7.4. From these results, we suggest that the urinary compounds are products of enzymatic degradation of compound (III) and that GSH may participate in the metabolism of urocanic acid, the first catabolite of L-histidine.
