Kinetic Experiment

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Guoli Shen - One of the best experts on this subject based on the ideXlab platform.

  • surface modified phanerochaete chrysosporium as a biosorbent for cr vi contaminated wastewater
    Journal of Hazardous Materials, 2011
    Co-Authors: Guiqiu Chen, Wenjuan Zhang, Guangming Zeng, Jinhui Huang, Liang Wang, Guoli Shen
    Abstract:

    To improve the removal efficiency of heavy metals from wastewater, the surface of a fungal biomass was modified to obtain a high-capacity biosorbent for Cr(VI) in wastewater. The effects of pH, initial concentration, and sorption time on Cr(VI) removal by polyethylenimine (PEI)-modified Phanerochaete chrysosporium were investigated. The biomass adsorption capacity was significantly dependent on the pH of the solution, and the optimum pH was approximately 3.0. The maximum removal for Cr(VI) was 344.8 mg/g as determined with the Langmuir adsorption isotherm. Pseudo-first-order Lagergren model is better than pseudo-second-order Lagergren model when simulating the Kinetic Experiment results. Furthermore, an amount of Cr(VI) was reduced to Cr(III), indicating that some reactions occurred on the surface of the biomass leading to the reduction of Cr(VI). The point of zero potential for the modified biomass increased from an initial pH of 3.0 to a much higher value of 10.8, indicating that the PEI-modified biomass is better than the pristine biomass for adsorption of anionic adsorbates. Results showed that the PEI-modified biosorbent presented high efficiency in treating Cr(VI)-contaminated wastewater.

Mahmood Aliofkhazraei - One of the best experts on this subject based on the ideXlab platform.

  • conductive microbial cellulose as a novel biocathode for cr vi bioreduction
    Carbohydrate Polymers, 2017
    Co-Authors: Mahshid Loloei, Alireza Sabour Roohaghdam, Abbas Rezaee, Mahmood Aliofkhazraei
    Abstract:

    Abstract In the present study, microbial cellulose (MC) as a carbohydrate polymer was made conductive by oxidative polymerization with aniline. Sulfate-reducing bacteria (SRB) were immobilized on the surface of the conductive biopolymer, and this was used as a biocathode in a bioreduction process to reduce Cr (VI) as a model of heavy metals. The results of Fourier transform infrared analysis confirmed that the polyaniline was distributed on the cellulose surface. The maximum tensile stress of the conductive biopolymer was obtained 23 MPa using calculating Young’s modulus. A current density of 60 mA/m 2 was determined as optimal, and an increase in pH from 5 to 7 significantly reduced the required time for reduction of Cr (VI). The system reached >99% removal of Cr (VI) within 1.5 h at pH 7. Kinetic Experiment studies showed a high constant rate (mean K obs 0.78, R 2 0.95). The results showed that the conductive MC can be used as an appropriate bioelectrode to reduce Cr (VI) in bioelectrochemical processes. It is expected that Experimental results could be used as a reference for the utilization of MC in bioelectrochemical systems.

Guiqiu Chen - One of the best experts on this subject based on the ideXlab platform.

  • surface modified phanerochaete chrysosporium as a biosorbent for cr vi contaminated wastewater
    Journal of Hazardous Materials, 2011
    Co-Authors: Guiqiu Chen, Wenjuan Zhang, Guangming Zeng, Jinhui Huang, Liang Wang, Guoli Shen
    Abstract:

    To improve the removal efficiency of heavy metals from wastewater, the surface of a fungal biomass was modified to obtain a high-capacity biosorbent for Cr(VI) in wastewater. The effects of pH, initial concentration, and sorption time on Cr(VI) removal by polyethylenimine (PEI)-modified Phanerochaete chrysosporium were investigated. The biomass adsorption capacity was significantly dependent on the pH of the solution, and the optimum pH was approximately 3.0. The maximum removal for Cr(VI) was 344.8 mg/g as determined with the Langmuir adsorption isotherm. Pseudo-first-order Lagergren model is better than pseudo-second-order Lagergren model when simulating the Kinetic Experiment results. Furthermore, an amount of Cr(VI) was reduced to Cr(III), indicating that some reactions occurred on the surface of the biomass leading to the reduction of Cr(VI). The point of zero potential for the modified biomass increased from an initial pH of 3.0 to a much higher value of 10.8, indicating that the PEI-modified biomass is better than the pristine biomass for adsorption of anionic adsorbates. Results showed that the PEI-modified biosorbent presented high efficiency in treating Cr(VI)-contaminated wastewater.

Roeckel-drevet Patricia - One of the best experts on this subject based on the ideXlab platform.

  • Early transcriptional response to gravistimulation in poplar without phototropic confounding factors
    'Oxford University Press (OUP)', 2021
    Co-Authors: Lopez David, Franchel Jérôme, Venisse Jean-stéphane, Drevet Joël, Label Philippe, Coutand Catherine, Roeckel-drevet Patricia
    Abstract:

    International audienceIn response to gravistimulation under anisotropic light, tree stems showing an active cambium produce reaction wood that redirects the axis of the trees. Several studies have described transcriptomic or proteomic models of reaction wood relative to the opposite wood. However, the mechanisms leading to the formation of reaction wood are difficult to decipher because so many environmental factors can induce various signalling pathways leading to this developmental reprogramming. Using an innovative isotropic device where the phototropic response does not interfere with gravistimulation we characterized the early molecular responses occurring in the stem of poplar after gravistimulation in an isotropic environment, and without deformation of the stem. After 30 min tilting at 35 degrees under anisotropic light, we collected the upper and lower xylems from the inclined stems. Controls were collected from vertical stems. We used a microarray approach to identify differentially expressed transcripts. High-throughput real-time PCR allowed a Kinetic Experiment at 0, 30, 120 and 180 min after tilting at 35 degrees, with candidate genes. We identified 668 differentially expressed transcripts, from which we selected 153 candidates for additional Fluidigm qPCR assessment. Five candidate co-expression gene clusters have been identified after the Kinetic monitoring of the expression of candidate genes. Gene ontology analyses indicate that molecular reprogramming of processes such as 'wood cell expansion', 'cell wall reorganization' and 'programmed cell death' occur as early as 30 min after gravistimulation. Of note is that the change in the expression of different genes involves a fine regulation of gibberellin and brassinosteroid pathways as well as flavonoid and phosphoinositide pathways. Our Experimental set-up allowed the identification of genes regulated in early gravitropic response without the bias introduced by phototropic and stem bending responses

  • Early transcriptional response to gravistimulation in poplar without phototropic confounding factors
    'Oxford University Press (OUP)', 2021
    Co-Authors: Lopez David, Franchel Jérôme, Venisse Jean-stéphane, Label Philippe, Coutand Catherine, Drevet Patricia, Roeckel-drevet Patricia
    Abstract:

    In response to gravistimulation under anisotropic light, tree stems showing an active cambium produce reaction wood that redirects the axis of the trees. Several studies have described transcriptomic or proteomic models of reaction wood relative to the opposite wood. However, the mechanisms leading to the formation of reaction wood are difficult to decipher because so many environmental factors can induce various signalling pathways leading to this developmental reprogramming. Using an innovative isotropic device where the phototropic response does not interfere with gravistimulation we characterized the early molecular responses occurring in the stem of poplar after gravistimulation in an isotropic environment, and without deformation of the stem. After 30 min tilting at 35° under anisotropic light, we collected the upper and lower xylems from the inclined stems. Controls were collected from vertical stems. We used a microarray approach to identify differentially expressed transcripts. High-throughput real-time PCR allowed a Kinetic Experiment at 0, 30, 120 and 180 min after tilting at 35°, with candidate genes. We identified 668 differentially expressed transcripts, from which we selected 153 candidates for additional Fluidigm qPCR assessment. Five candidate co-expression gene clusters have been identified after the Kinetic monitoring of the expression of candidate genes. Gene ontology analyses indicate that molecular reprogramming of processes such as 'wood cell expansion', 'cell wall reorganization' and 'programmed cell death' occur as early as 30 min after gravistimulation. Of note is that the change in the expression of different genes involves a fine regulation of gibberellin and brassinosteroid pathways as well as flavonoid and phosphoinositide pathways. Our Experimental set-up allowed the identification of genes regulated in early gravitropic response without the bias introduced by phototropic and stem bending responses

Liang Wang - One of the best experts on this subject based on the ideXlab platform.

  • surface modified phanerochaete chrysosporium as a biosorbent for cr vi contaminated wastewater
    Journal of Hazardous Materials, 2011
    Co-Authors: Guiqiu Chen, Wenjuan Zhang, Guangming Zeng, Jinhui Huang, Liang Wang, Guoli Shen
    Abstract:

    To improve the removal efficiency of heavy metals from wastewater, the surface of a fungal biomass was modified to obtain a high-capacity biosorbent for Cr(VI) in wastewater. The effects of pH, initial concentration, and sorption time on Cr(VI) removal by polyethylenimine (PEI)-modified Phanerochaete chrysosporium were investigated. The biomass adsorption capacity was significantly dependent on the pH of the solution, and the optimum pH was approximately 3.0. The maximum removal for Cr(VI) was 344.8 mg/g as determined with the Langmuir adsorption isotherm. Pseudo-first-order Lagergren model is better than pseudo-second-order Lagergren model when simulating the Kinetic Experiment results. Furthermore, an amount of Cr(VI) was reduced to Cr(III), indicating that some reactions occurred on the surface of the biomass leading to the reduction of Cr(VI). The point of zero potential for the modified biomass increased from an initial pH of 3.0 to a much higher value of 10.8, indicating that the PEI-modified biomass is better than the pristine biomass for adsorption of anionic adsorbates. Results showed that the PEI-modified biosorbent presented high efficiency in treating Cr(VI)-contaminated wastewater.