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Brian Austin - One of the best experts on this subject based on the ideXlab platform.
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properties of probiotics Kocuria sm1 and rhodococcus sm2 isolated from fish guts
Probiotics and Antimicrobial Proteins, 2018Co-Authors: S M Sharifuzzaman, Brian Austin, Hafizur Rahman, D A AustinAbstract:This study characterized probiotics Kocuria SM1 and Rhodococcus SM2, which were recovered from the intestinal microbiota of rainbow trout (Oncorhynchus mykiss, Walbaum). The cultures were Gram-positive, non-motile, catalase-positive and oxidase-negative cocci or rods. Cell multiplication of SM1 and SM2 was observed at 4–37 °C (45 °C for SM1), in 0–20% (w/v) NaCl and at pH 2–11. The viability was not affected when exposed to pepsin at pH 2.0 and 3.0, and pancreatin at pH 8.0. Neither isolates were chrome azurol S-positive for siderophore production. Of the 19 common enzymes analysed using the API-ZYM system, only 8 were evident in the culture of SM1 compared to 11 enzymes for SM2. The secondary metabolites of both probiotics were inhibitory to Acinetobacter baumannii, Vibrio anguillarum and V. ordalii; SM2 inhibited Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus. SM2 was resistant to penicillin and sulphatriad, out of six antimicrobial agents; SM1 was resistant to sulphatriad. These results suggest that Kocuria SM1 and Rhodococcus SM2 are able to grow over a wide range of temperature, salinity and pH, including in conditions that mimic the gastrointestinal environment of fish and produce extracellular enzymes that may have a role in the host digestive processes. Importantly, Rhodococcus SM2 displays a high degree of bacteriocinogenic potential against multi-drug-resistant human pathogens that have never been documented among the gut microbiota of fish.
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development of protection in rainbow trout oncorhynchus mykiss walbaum to vibrio anguillarum following use of the probiotic Kocuria sm1
Fish & Shellfish Immunology, 2010Co-Authors: S M Sharifuzzaman, Brian AustinAbstract:Probiotic Kocuria SM1, which was part of the allochthonous gut microbiota of rainbow trout, was administered to rainbow trout (Oncorhynchus mykiss) fingerlings as dietary supplement at ∼108 cells g−1 for two weeks. The fish were challenged with Vibrio anguillarum and innate immunity determined over the following 5 weeks. The probiotic-fed fish survived (mortalities = 10–28%; P < 0.05) better than the controls (mortalities = 73–92%) for 5 weeks after stopping administration of Kocuria SM1, although the relative percent survival (RPS) declined steadily from 87 to 36% by the end of the experiment. A significant (P < 0.05) effect on the immune parameters was observed in fish within 3 weeks after stopping administration of Kocuria SM1 in comparison with the controls, i.e. enhancement of the respiratory burst (OD550nm) from 0.051 ± 0.014 to 0.067 ± 0.009, lysozyme 605 ± 185 units ml−1 to 872 ± 114 units ml−1, total protein 19.8 ± 2.0 mg ml−1 to 23.8 ± 0.8 mg ml−1 at 2 weeks, and leukocrit 2.9 ± 1.8% to 5.1 ± 1.1%, peroxidase (OD550nm) 0.23 ± 0.02 to 0.37 ± 0.11 and bacterial killing activities (percentage of surviving bacteria = 82 ± 12% to 57 ± 4%) at 3 weeks. Generally, the levels reduced progressively towards the end of experiment.
S M Sharifuzzaman - One of the best experts on this subject based on the ideXlab platform.
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properties of probiotics Kocuria sm1 and rhodococcus sm2 isolated from fish guts
Probiotics and Antimicrobial Proteins, 2018Co-Authors: S M Sharifuzzaman, Brian Austin, Hafizur Rahman, D A AustinAbstract:This study characterized probiotics Kocuria SM1 and Rhodococcus SM2, which were recovered from the intestinal microbiota of rainbow trout (Oncorhynchus mykiss, Walbaum). The cultures were Gram-positive, non-motile, catalase-positive and oxidase-negative cocci or rods. Cell multiplication of SM1 and SM2 was observed at 4–37 °C (45 °C for SM1), in 0–20% (w/v) NaCl and at pH 2–11. The viability was not affected when exposed to pepsin at pH 2.0 and 3.0, and pancreatin at pH 8.0. Neither isolates were chrome azurol S-positive for siderophore production. Of the 19 common enzymes analysed using the API-ZYM system, only 8 were evident in the culture of SM1 compared to 11 enzymes for SM2. The secondary metabolites of both probiotics were inhibitory to Acinetobacter baumannii, Vibrio anguillarum and V. ordalii; SM2 inhibited Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus. SM2 was resistant to penicillin and sulphatriad, out of six antimicrobial agents; SM1 was resistant to sulphatriad. These results suggest that Kocuria SM1 and Rhodococcus SM2 are able to grow over a wide range of temperature, salinity and pH, including in conditions that mimic the gastrointestinal environment of fish and produce extracellular enzymes that may have a role in the host digestive processes. Importantly, Rhodococcus SM2 displays a high degree of bacteriocinogenic potential against multi-drug-resistant human pathogens that have never been documented among the gut microbiota of fish.
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development of protection in rainbow trout oncorhynchus mykiss walbaum to vibrio anguillarum following use of the probiotic Kocuria sm1
Fish & Shellfish Immunology, 2010Co-Authors: S M Sharifuzzaman, Brian AustinAbstract:Probiotic Kocuria SM1, which was part of the allochthonous gut microbiota of rainbow trout, was administered to rainbow trout (Oncorhynchus mykiss) fingerlings as dietary supplement at ∼108 cells g−1 for two weeks. The fish were challenged with Vibrio anguillarum and innate immunity determined over the following 5 weeks. The probiotic-fed fish survived (mortalities = 10–28%; P < 0.05) better than the controls (mortalities = 73–92%) for 5 weeks after stopping administration of Kocuria SM1, although the relative percent survival (RPS) declined steadily from 87 to 36% by the end of the experiment. A significant (P < 0.05) effect on the immune parameters was observed in fish within 3 weeks after stopping administration of Kocuria SM1 in comparison with the controls, i.e. enhancement of the respiratory burst (OD550nm) from 0.051 ± 0.014 to 0.067 ± 0.009, lysozyme 605 ± 185 units ml−1 to 872 ± 114 units ml−1, total protein 19.8 ± 2.0 mg ml−1 to 23.8 ± 0.8 mg ml−1 at 2 weeks, and leukocrit 2.9 ± 1.8% to 5.1 ± 1.1%, peroxidase (OD550nm) 0.23 ± 0.02 to 0.37 ± 0.11 and bacterial killing activities (percentage of surviving bacteria = 82 ± 12% to 57 ± 4%) at 3 weeks. Generally, the levels reduced progressively towards the end of experiment.
Jinwoo Bae - One of the best experts on this subject based on the ideXlab platform.
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Kocuria salsicia sp nov isolated from salt fermented seafood
International Journal of Systematic and Evolutionary Microbiology, 2011Co-Authors: Jihyun Yun, Eunjin Park, Minsoo Kim, Seong Woon Roh, Mija Jung, Keesun Shin, Youngdo Nam, Jinwoo BaeAbstract:Strain 104T was isolated from a traditional salt-fermented seafood in Korea. It was a Gram-positive, non-motile, coccus-shaped bacterium. It formed lemon–yellow, opaque colonies that were circular with entire margins. Optimal growth occurred at 30–37 °C, pH 7–8 and in the presence of 0–2 % (w/v) NaCl. Phylogenetic analysis of 16S rRNA gene sequences from strain 104T and reference species of the genus Kocuria indicated that strain 104T formed an independent line. The G+C content of the chromosomal DNA was 60.6 mol%. MK-7 was the major menaquinone and the predominant fatty acids were anteiso-C15 : 0 (76.7 %), anteiso-C17 : 0 (10.9 %) and iso-C16 : 0 (4.5 %). Strain 104T was most closely related to Kocuria rhizophila TA68T (98.9 % 16S rRNA gene sequence similarity). The DNA–DNA hybridization value between strain 104T and K. rhizophila TA68T was 14.1±3.4 %. On the basis of this polyphasic taxonomic analysis, strain 104T appears to represent a novel species in the genus Kocuria. The name Kocuria salsicia sp. nov. is proposed. The type strain is 104T (=KACC 21128T=JCM 16361T).
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Kocuria atrinae sp nov isolated from traditional korean fermented seafood
International Journal of Systematic and Evolutionary Microbiology, 2010Co-Authors: Eunjin Park, Minsoo Kim, Seong Woon Roh, Mija Jung, Jinwoo BaeAbstract:A novel actinobacterium, strain P30(T), was isolated from jeotgal, a traditional Korean fermented seafood. Cells were aerobic, Gram-positive, non-motile and coccoid. Optimal growth occurred at 30-37 degrees C, at pH 8-9 and in the presence of 0-2 % (w/v) NaCl. Based on 16S rRNA gene sequence analysis, strain P30(T) was phylogenetically closely related to Kocuria carniphila, Kocuria gwangalliensis, Kocuria rhizophila, Kocuria marina, Kocuria rosea and K. varians with levels of similarity of 98.6, 98.2, 98.1, 97.4, 97.3 and 97.3 %, respectively, to the type strains of these species. Levels of DNA-DNA relatedness between strain P30(T) and the type strains of K. carniphila, K. rhizophila, K. marina, K. rosea and K. varians were 37, 43, 37, 25 and 17 %, respectively. The predominant menaquinone of strain P30(T) was MK-7. Major cellular fatty acids were anteiso-C(15 : 0), iso-C(15 : 0) and iso-C(16 : 0). The genomic DNA G+C content of strain P30(T) was 70.2 mol%. Based on these data, strain P30(T) is considered to represent a novel species of the genus Kocuria, for which the name Kocuria atrinae sp. nov. is proposed. The type strain is P30(T) (=KCTC 19594(T)=JCM 15914(T)).
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Kocuria koreensis sp nov isolated from fermented seafood
International Journal of Systematic and Evolutionary Microbiology, 2010Co-Authors: Eunjin Park, Minsoo Kim, Seong Woon Roh, Mija Jung, Keesun Shin, Jinwoo BaeAbstract:A Gram-positive, aerobic, non-motile and coccoid actinobacterium, designated P31T, was isolated from a traditional, fermented seafood. The strain was catalase-positive and oxidase-negative. Cells grew in the presence of 0–15.0 % (w/v) NaCl, and at pH 5–10 and 15–37 °C. Major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. Strain P31T contained MK-7 as the predominant menaquinone. The DNA G+C content of the genomic DNA of strain P31T was 65.2 mol%. A phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain P31T was most closely related to Kocuria kristinae DSM 20032T, with 96.9 % similarity, and these two strains clustered together in constructed phylogenetic trees. The DNA–DNA hybridization value between strain P31T and K. kristinae DSM 20032T was 21.1 %. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, it is suggested that strain P31T represents a novel species of the genus Kocuria, for which the name Kocuria koreensis sp. nov. is proposed. The type strain is P31T (=KCTC 19595T=JCM 15915T).
Yehuda Carmeli - One of the best experts on this subject based on the ideXlab platform.
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vitek 2 automated identification system and Kocuria kristinae
Journal of Clinical Microbiology, 2005Co-Authors: Michael Boudewijns, Ronen Benami, J Vandeven, Jan Verhaegen, Yehuda CarmeliAbstract:We have read with great interest the article by Ben-Ami et al. ([2][1]) about the misidentification of coagulase-negative staphylococci (CNoS) as Kocuria spp. by the Vitek 2 system (bioMerieux, Marcy l'Etoile, France). They warned that a clinical specimen growing Kocuria should raise suspicion of
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erroneous reporting of coagulase negative staphylococci as Kocuria spp by the vitek 2 system
Journal of Clinical Microbiology, 2005Co-Authors: Ronen Benami, Shiri Navonvenezia, David A Schwartz, Y Schlezinger, Y Mekuzas, Yehuda CarmeliAbstract:Misidentification of coagulase-negative staphylococci (CoNS) may delay appropriate treatment. We investigated 20 clinical isolates identified as Kocuria spp. by the Vitek 2 system. All were identified as CoNS by 16S rRNA gene sequencing (18 Staphylococcus epidermidis, 1 Staphylococcus haemolyticus). Four Kocuria isolates were shown to be identical to CoNS from the same patient by pulsed-field gel electrophoresis. Isolates identified by Vitek 2 as Kocuria most likely represent misidentified CoNS, and if clinically indicated, should be investigated further by genomic methods.
Michael Wink - One of the best experts on this subject based on the ideXlab platform.
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Kocuria tytonicola new bacteria from the preen glands of american barn owls tyto furcata
Systematic and Applied Microbiology, 2019Co-Authors: Markus Santhosh Braun, Erjia Wang, Stefan Zimmermann, Hermann Wagner, Sebastien Boutin, Michael WinkAbstract:Abstract Although birds are hosts to a large number of microorganisms, microbes have rarely been found in avian oil glands. Here, we report on two strains of a new bacterial species from the preen oil of American barn owls (Tyto furcata). Phenotypic as well as genotypic methods placed the isolates to the genus Kocuria. Strains are non-fastidious, non-lipophilic Gram-positive cocci and can be unambiguously discriminated from their closest relative Kocuria rhizophila DSM 11926T. In phylogenetic trees, the owl bacteria formed a distinct cluster which was clearly separated from all other known Kocuria species. The same conclusion was drawn from MALDI-TOF MS analyses. Once again, the new bacterial strains were very similar to one another, but exhibited substantial differences when compared to the most closely related species. Besides, the results of the biochemical tests, optimum growth conditions and pigmentation differed from closely related Kocuria spp. Finally, ANIb values of less than 87% provided striking evidence that the isolates recovered from American barn owls represent a hitherto undescribed species, for which we propose the name Kocuria tytonicola sp. nov. The type strain is 489T (DSM 104133T = LMG 29945T, taxonumber TA00340).
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Kocuria tytonis sp. nov., isolated from the uropygial gland of an American barn owl (Tyto furcata).
International journal of systematic and evolutionary microbiology, 2019Co-Authors: Markus Santhosh Braun, Erjia Wang, Stefan Zimmermann, Hermann Wagner, Michael WinkAbstract:Avian uropygial glands have received increasing attention in recent years, but little is known about micro-organisms in uropygial glands. In this study, we isolated a strain of Gram-stain-positive, non-motile, non-spore-forming cocci, designated 442T, from the uropygial gland of an American barn owl (Tyto furcata) and characterized it using a polyphasic approach. 16S rRNA gene sequence analysis placed the isolate in the genus Kocuria. The G+C content was 70.8 mol%, the major menaquinone was MK-7(H2) and the predominant cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C15 : 0. Phylogenetic analyses based on the 16S rRNA gene identified Kocuria rhizophila DSM 11926T (99.6 % similarity), Kocuria salsicia DSM 24776T (98.7 %), Kocuria varians DSM 20033T (98.3 %) and Kocuria marina DSM 16420T (98.3 %) as the most closely related species. However, average nucleotide identity values below 86 % indicated that the isolate differed from all species hitherto described. Chemotaxonomic analyses and whole-cell protein profiles corroborated these findings. Accordingly, the isolate is considered to be a member of a novel species, for which the name Kocuria tytonis sp. nov. is proposed. The type strain is 442T (=DSM 104130T=LMG 29944T).
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Kocuria uropygioeca sp nov and Kocuria uropygialis sp nov isolated from the preen glands of great spotted woodpeckers dendrocopos major
Systematic and Applied Microbiology, 2017Co-Authors: Markus Santhosh Braun, Erjia Wang, Stefan Zimmermann, Sebastien Boutin, Michael WinkAbstract:Abstract Two new species of Gram-positive cocci were isolated from the uropygial glands of wild woodpeckers (Dendrocopos major) originating from different locations in Germany. A polyphasic approach confirmed the affiliation of the isolates to the genus Kocuria. Phylogenetic analysis based on the 16S rRNA gene showed high degree of similarity to Kocuria koreensis DSM 23367T (99.0% for both isolates). However, low ANIb values of
