The Experts below are selected from a list of 264 Experts worldwide ranked by ideXlab platform
Diane Davis Davey - One of the best experts on this subject based on the ideXlab platform.
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Accuracy of ThinPrep Imaging System in Detecting Low-Grade Squamous Intraepithelial Lesions
Archives of Pathology & Laboratory Medicine, 2007Co-Authors: Fred Fangyuan Zhang, Helen Whitler Banks, Shanna Maria Langford, Diane Davis DaveyAbstract:Abstract Context.—The ThinPrep Imaging System (Imager) for cervical cytology is used in many US laboratories, but the ability of the system to identify classic changes of low-grade squamous intraepithelial lesion (LSIL) has not been independently reported. Objective.—To evaluate the accuracy of this system in detecting classic LSIL cells. Design.—A total of 114 imaged ThinPrep LSIL cases from April to June 2005 were reviewed to determine whether the most diagnostically relevant cells were present in the 22 fields selected by Imager. Those LSIL specimens from January to June 2005 that were initially screened by the Imager and classified as “negative” in the 22 fields were also reviewed. The average number of classic Koilocytes per slide was compared among cases that had Koilocytes within 22 fields with cases upgraded during full review. Results.—Twelve (10.5%) of 114 LSIL cases from April to June 2005 did not show diagnostic LSIL cells within the initial 22 fields. Full manual rescreening was performed on 1025 cases from January to June (20.4% of negative cases). Nine cases that were initially negative in the 22 fields were revised to LSIL (2.4% of all 381 LSIL cases reported from January to June 2005). An average of 10 to 11 Koilocytes were present in these 2 groups, which was significantly lower than the average of 75 Koilocytes in cases in which the 22 Imager-selected fields showed LSIL. Conclusions.—Although the ThinPrep Imager finds abnormal cells in most LSIL cases, the system may have limitations in detecting Koilocytes in the 22 Imager-selected fields. When 10% quality control rescreening is performed as required by federal regulations, full manual rescreening will provide the most accurate results.
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Accuracy of ThinPrep Imaging System in detecting low-grade squamous intraepithelial lesions.
Archives of pathology & laboratory medicine, 2007Co-Authors: Fred Fangyuan Zhang, Helen Whitler Banks, Shanna Maria Langford, Diane Davis DaveyAbstract:Abstract Context.—The ThinPrep Imaging System (Imager) for cervical cytology is used in many US laboratories, but the ability of the system to identify classic changes of low-grade squamous intraepithelial lesion (LSIL) has not been independently reported. Objective.—To evaluate the accuracy of this system in detecting classic LSIL cells. Design.—A total of 114 imaged ThinPrep LSIL cases from April to June 2005 were reviewed to determine whether the most diagnostically relevant cells were present in the 22 fields selected by Imager. Those LSIL specimens from January to June 2005 that were initially screened by the Imager and classified as “negative” in the 22 fields were also reviewed. The average number of classic Koilocytes per slide was compared among cases that had Koilocytes within 22 fields with cases upgraded during full review. Results.—Twelve (10.5%) of 114 LSIL cases from April to June 2005 did not show diagnostic LSIL cells within the initial 22 fields. Full manual rescreening was performed on ...
Michal Michal - One of the best experts on this subject based on the ideXlab platform.
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Human papillomavirus infection and p16 expression in the immunocompetent patients with extragenital/extraungual Bowen’s disease
Diagnostic Pathology, 2016Co-Authors: Marián Švajdler, Roman Mezencev, Jana Kašpírková, Denisa Kacerovská, Dmitry V. Kazakov, Ondrej Ondič, Michal MichalAbstract:Background The role of human papillomaviruses (HPV) in the development of squamous cell carcinoma (SCC) has been established for anogenital lesions but still remains controversial for carcinomas in other sites. The aim of this study was to determine the α-HPV and β-HPV prevalence and their association with p16 expression, sun exposure, and clinicopathological findings in patients with Bowen’s disease (BD). Methods One hundred sixty nine skin biopsy specimens from 157 immunocompetent patients with extragenital/extraungual BD were examined for HPV status and p16 expression. The presence of Koilocyte-like changes, solar elastosis and papillomatosis was recorded for each specimen. Results BD was diagnosed more often in potentially sun-exposed sites with prevalence 73.6 % and a remarkable predilection for the head and neck region. High risk α-HPV or β-HPV were detected in 34.7 % of lesions and β-HPV infections dominated over α-HPV. Higher prevalence of Koilocyte-like changes and papillomatosis was found in HPV-positive specimens but it was not statistically significant. The expression of p16 was detected in 79.8 % of lesions and displayed no correlation with the HPV status. HPV-positivity tended to be detected more often in sun-protected sites. Dual infections by α-HPV/β-HPV genera and mixed α-HPV infections were not detected, while 37.5 % of β-HPV positive specimens were infected by two or more β-HPV genotypes. HPV 9 was significantly associated with mixed β-HPV infections. Conclusions HPV may play an etiological role at least in some SCC in situ arising in extragenital sites. Sunprotected sites may be more dependent on HPV-mediated co-carcinogenesis than sun exposed areas. The presence of the p16-expression, papillomatosis or Koilocyte-like change is not a reliable marker of HPV infection in SCC in situ.
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Human papillomavirus infection and p16 expression in the immunocompetent patients with extragenital/extraungual Bowen’s disease
Diagnostic pathology, 2016Co-Authors: Marián Švajdler, Roman Mezencev, Jana Kašpírková, Denisa Kacerovská, Dmitry V. Kazakov, Ondrej Ondič, Michal MichalAbstract:The role of human papillomaviruses (HPV) in the development of squamous cell carcinoma (SCC) has been established for anogenital lesions but still remains controversial for carcinomas in other sites. The aim of this study was to determine the α-HPV and β-HPV prevalence and their association with p16 expression, sun exposure, and clinicopathological findings in patients with Bowen’s disease (BD). One hundred sixty nine skin biopsy specimens from 157 immunocompetent patients with extragenital/extraungual BD were examined for HPV status and p16 expression. The presence of Koilocyte-like changes, solar elastosis and papillomatosis was recorded for each specimen. BD was diagnosed more often in potentially sun-exposed sites with prevalence 73.6 % and a remarkable predilection for the head and neck region. High risk α-HPV or β-HPV were detected in 34.7 % of lesions and β-HPV infections dominated over α-HPV. Higher prevalence of Koilocyte-like changes and papillomatosis was found in HPV-positive specimens but it was not statistically significant. The expression of p16 was detected in 79.8 % of lesions and displayed no correlation with the HPV status. HPV-positivity tended to be detected more often in sun-protected sites. Dual infections by α-HPV/β-HPV genera and mixed α-HPV infections were not detected, while 37.5 % of β-HPV positive specimens were infected by two or more β-HPV genotypes. HPV 9 was significantly associated with mixed β-HPV infections. HPV may play an etiological role at least in some SCC in situ arising in extragenital sites. Sunprotected sites may be more dependent on HPV-mediated co-carcinogenesis than sun exposed areas. The presence of the p16-expression, papillomatosis or Koilocyte-like change is not a reliable marker of HPV infection in SCC in situ.
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Spiradenocylindroma-like basaloid carcinoma of the anus and rectum: case report, including HPV studies and analysis of the CYLD gene mutations.
The American Journal of dermatopathology, 2008Co-Authors: Denisa Kacerovská, Michal Michal, Szépe P, Tomas Vanecek, Jana Nemcova, Petr Mukensnabl, Dmitry V. KazakovAbstract:The authors report a case of basaloid carcinoma involving the anus and rectum of a 57-year-old woman. Microscopically, the tumor showed unusual morphologic features strongly resembling a spiradenocylindroma because it consisted, in most parts, of basaloid cell nodules arranged in a jigsaw-puzzle fashion containing or surrounded by eosinophilic basal membrane material; in addition, there were intratumoral lymphocytes. The overlying squamous epithelium manifested dysplastic changes compatible with in situ squamous carcinoma that gradually became invasive and blended with basaloid cell islands; additionally, there were Koilocytes in the squamous epithelium. A molecular biology study identified HPV-16 in the lesional tissue. Analysis of the CYLD gene did not prove any mutation.
Fred Fangyuan Zhang - One of the best experts on this subject based on the ideXlab platform.
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Accuracy of ThinPrep Imaging System in Detecting Low-Grade Squamous Intraepithelial Lesions
Archives of Pathology & Laboratory Medicine, 2007Co-Authors: Fred Fangyuan Zhang, Helen Whitler Banks, Shanna Maria Langford, Diane Davis DaveyAbstract:Abstract Context.—The ThinPrep Imaging System (Imager) for cervical cytology is used in many US laboratories, but the ability of the system to identify classic changes of low-grade squamous intraepithelial lesion (LSIL) has not been independently reported. Objective.—To evaluate the accuracy of this system in detecting classic LSIL cells. Design.—A total of 114 imaged ThinPrep LSIL cases from April to June 2005 were reviewed to determine whether the most diagnostically relevant cells were present in the 22 fields selected by Imager. Those LSIL specimens from January to June 2005 that were initially screened by the Imager and classified as “negative” in the 22 fields were also reviewed. The average number of classic Koilocytes per slide was compared among cases that had Koilocytes within 22 fields with cases upgraded during full review. Results.—Twelve (10.5%) of 114 LSIL cases from April to June 2005 did not show diagnostic LSIL cells within the initial 22 fields. Full manual rescreening was performed on 1025 cases from January to June (20.4% of negative cases). Nine cases that were initially negative in the 22 fields were revised to LSIL (2.4% of all 381 LSIL cases reported from January to June 2005). An average of 10 to 11 Koilocytes were present in these 2 groups, which was significantly lower than the average of 75 Koilocytes in cases in which the 22 Imager-selected fields showed LSIL. Conclusions.—Although the ThinPrep Imager finds abnormal cells in most LSIL cases, the system may have limitations in detecting Koilocytes in the 22 Imager-selected fields. When 10% quality control rescreening is performed as required by federal regulations, full manual rescreening will provide the most accurate results.
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Accuracy of ThinPrep Imaging System in detecting low-grade squamous intraepithelial lesions.
Archives of pathology & laboratory medicine, 2007Co-Authors: Fred Fangyuan Zhang, Helen Whitler Banks, Shanna Maria Langford, Diane Davis DaveyAbstract:Abstract Context.—The ThinPrep Imaging System (Imager) for cervical cytology is used in many US laboratories, but the ability of the system to identify classic changes of low-grade squamous intraepithelial lesion (LSIL) has not been independently reported. Objective.—To evaluate the accuracy of this system in detecting classic LSIL cells. Design.—A total of 114 imaged ThinPrep LSIL cases from April to June 2005 were reviewed to determine whether the most diagnostically relevant cells were present in the 22 fields selected by Imager. Those LSIL specimens from January to June 2005 that were initially screened by the Imager and classified as “negative” in the 22 fields were also reviewed. The average number of classic Koilocytes per slide was compared among cases that had Koilocytes within 22 fields with cases upgraded during full review. Results.—Twelve (10.5%) of 114 LSIL cases from April to June 2005 did not show diagnostic LSIL cells within the initial 22 fields. Full manual rescreening was performed on ...
Marián Švajdler - One of the best experts on this subject based on the ideXlab platform.
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Human papillomavirus infection and p16 expression in the immunocompetent patients with extragenital/extraungual Bowen’s disease
Diagnostic Pathology, 2016Co-Authors: Marián Švajdler, Roman Mezencev, Jana Kašpírková, Denisa Kacerovská, Dmitry V. Kazakov, Ondrej Ondič, Michal MichalAbstract:Background The role of human papillomaviruses (HPV) in the development of squamous cell carcinoma (SCC) has been established for anogenital lesions but still remains controversial for carcinomas in other sites. The aim of this study was to determine the α-HPV and β-HPV prevalence and their association with p16 expression, sun exposure, and clinicopathological findings in patients with Bowen’s disease (BD). Methods One hundred sixty nine skin biopsy specimens from 157 immunocompetent patients with extragenital/extraungual BD were examined for HPV status and p16 expression. The presence of Koilocyte-like changes, solar elastosis and papillomatosis was recorded for each specimen. Results BD was diagnosed more often in potentially sun-exposed sites with prevalence 73.6 % and a remarkable predilection for the head and neck region. High risk α-HPV or β-HPV were detected in 34.7 % of lesions and β-HPV infections dominated over α-HPV. Higher prevalence of Koilocyte-like changes and papillomatosis was found in HPV-positive specimens but it was not statistically significant. The expression of p16 was detected in 79.8 % of lesions and displayed no correlation with the HPV status. HPV-positivity tended to be detected more often in sun-protected sites. Dual infections by α-HPV/β-HPV genera and mixed α-HPV infections were not detected, while 37.5 % of β-HPV positive specimens were infected by two or more β-HPV genotypes. HPV 9 was significantly associated with mixed β-HPV infections. Conclusions HPV may play an etiological role at least in some SCC in situ arising in extragenital sites. Sunprotected sites may be more dependent on HPV-mediated co-carcinogenesis than sun exposed areas. The presence of the p16-expression, papillomatosis or Koilocyte-like change is not a reliable marker of HPV infection in SCC in situ.
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Human papillomavirus infection and p16 expression in the immunocompetent patients with extragenital/extraungual Bowen’s disease
Diagnostic pathology, 2016Co-Authors: Marián Švajdler, Roman Mezencev, Jana Kašpírková, Denisa Kacerovská, Dmitry V. Kazakov, Ondrej Ondič, Michal MichalAbstract:The role of human papillomaviruses (HPV) in the development of squamous cell carcinoma (SCC) has been established for anogenital lesions but still remains controversial for carcinomas in other sites. The aim of this study was to determine the α-HPV and β-HPV prevalence and their association with p16 expression, sun exposure, and clinicopathological findings in patients with Bowen’s disease (BD). One hundred sixty nine skin biopsy specimens from 157 immunocompetent patients with extragenital/extraungual BD were examined for HPV status and p16 expression. The presence of Koilocyte-like changes, solar elastosis and papillomatosis was recorded for each specimen. BD was diagnosed more often in potentially sun-exposed sites with prevalence 73.6 % and a remarkable predilection for the head and neck region. High risk α-HPV or β-HPV were detected in 34.7 % of lesions and β-HPV infections dominated over α-HPV. Higher prevalence of Koilocyte-like changes and papillomatosis was found in HPV-positive specimens but it was not statistically significant. The expression of p16 was detected in 79.8 % of lesions and displayed no correlation with the HPV status. HPV-positivity tended to be detected more often in sun-protected sites. Dual infections by α-HPV/β-HPV genera and mixed α-HPV infections were not detected, while 37.5 % of β-HPV positive specimens were infected by two or more β-HPV genotypes. HPV 9 was significantly associated with mixed β-HPV infections. HPV may play an etiological role at least in some SCC in situ arising in extragenital sites. Sunprotected sites may be more dependent on HPV-mediated co-carcinogenesis than sun exposed areas. The presence of the p16-expression, papillomatosis or Koilocyte-like change is not a reliable marker of HPV infection in SCC in situ.
J S Lawson - One of the best experts on this subject based on the ideXlab platform.
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Human papillomavirus and Epstein Barr virus in prostate cancer: Koilocytes indicate potential oncogenic influences of human papillomavirus in prostate cancer
The Prostate, 2012Co-Authors: N J Whitaker, W K Glenn, Arisha Sahrudin, Matthew M. Orde, Warick Delprado, J S LawsonAbstract:INTRODUCTION The purpose of this study is to determine if high risk human papillomaviruses (HPV) and Epstein Barr virus (EBV) are both present in the same prostate cancer specimens. METHODS We used a range of analytical techniques including in situ polymerase chain reaction (IS-PCR) and standard liquid PCR followed by sequencing of the product to seek to identify HPV and EBV in normal, benign, and malignant prostate tissues. RESULTS Both HPV type 18 and EBV gene sequences were identified in a high and approximately equal proportion of normal, benign, and prostate cancer specimens. These sequences were located in the nuclei of prostate epithelial cells. HPV associated Koilocytes were identified in 24% of prostate cancer specimens. CONCLUSIONS The presence of both HPV and EBV gene sequences in most of the same normal, benign, and malignant prostate specimens is particularly noteworthy because of recent experimental evidence demonstrating that EBV and HPV can collaborate to increase proliferation of cultured cervical cells. Because the presence of EBV and HPV in normal, benign, and malignant prostate tissues appears to be ubiquitous, it is possible that they are harmless. On the other hand HPV type 18 in particular, has high oncogenic potential and may be associated with some prostate cancers. The identification of HPV associated Koilocytes in prostate cancer specimens is an indication of HPV infection and potential oncogenic influences of human papillomavirus in prostate cancer. Prostate 73: 236–241, 2013. © 2012 Wiley Periodicals, Inc.
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Reply: Koilocytes indicate a role for human papilloma virus in breast cancer
British Journal of Cancer, 2010Co-Authors: W K Glenn, B Heng, N J Whitaker, J S LawsonAbstract:1 page(s
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Koilocytes indicate a role for human papilloma virus in breast cancer
British Journal of Cancer, 2009Co-Authors: J S Lawson, W K Glenn, B Heng, B Tran, L Lutze-mann, N J WhitakerAbstract:Background: High-risk human papilloma viruses (HPVs) are candidates as causal viruses in breast cancer. The scientific challenge is to determine whether HPVs are causal and not merely passengers or parasites. Studies of HPV-related Koilocytes in breast cancer offer an opportunity to address this crucial issue. Koilocytes are epithelial cells characterised by perinuclear haloes surrounding condensed nuclei and are commonly present in cervical intraepithelial neoplasia. Koilocytosis is accepted as pathognomonic (characteristic of a particular disease) of HPV infection. The aim of this investigation is to determine whether putative Koilocytes in normal and malignant breast tissues are because of HPV infection. Methods: Archival formalin-fixed normal and malignant breast specimens were investigated by histology, in situ PCR with confirmation of the findings by standard PCR and sequencing of the products, plus immunohistochemistry to identify HPV E6 oncoproteins. Results: human papilloma virus-associated Koilocytes were present in normal breast skin and lobules and in the breast skin and cancer tissue of patients with ductal carcinoma in situ (DCIS) and invasive ductal carcinomas (IDCs). Interpretation: As Koilocytes are known to be the precursors of some HPV-associated cervical cancer, it follows that HPVs may be causally associated with breast cancer.
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Koilocytes indicate a role for human papilloma virus in breast cancer.
British journal of cancer, 2009Co-Authors: J S Lawson, W K Glenn, B Heng, B Tran, L Lutze-mann, N J WhitakerAbstract:High-risk human papilloma viruses (HPVs) are candidates as causal viruses in breast cancer. The scientific challenge is to determine whether HPVs are causal and not merely passengers or parasites. Studies of HPV-related Koilocytes in breast cancer offer an opportunity to address this crucial issue. Koilocytes are epithelial cells characterised by perinuclear haloes surrounding condensed nuclei and are commonly present in cervical intraepithelial neoplasia. Koilocytosis is accepted as pathognomonic (characteristic of a particular disease) of HPV infection. The aim of this investigation is to determine whether putative Koilocytes in normal and malignant breast tissues are because of HPV infection. Archival formalin-fixed normal and malignant breast specimens were investigated by histology, in situ PCR with confirmation of the findings by standard PCR and sequencing of the products, plus immunohistochemistry to identify HPV E6 oncoproteins. human papilloma virus-associated Koilocytes were present in normal breast skin and lobules and in the breast skin and cancer tissue of patients with ductal carcinoma in situ (DCIS) and invasive ductal carcinomas (IDCs). As Koilocytes are known to be the precursors of some HPV-associated cervical cancer, it follows that HPVs may be causally associated with breast cancer.
