The Experts below are selected from a list of 1821579 Experts worldwide ranked by ideXlab platform
Alipasha Vaziri - One of the best experts on this subject based on the ideXlab platform.
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brain wide 3d imaging of neuronal activity in caenorhabditis elegans with sculpted light
Nature Methods, 2013Co-Authors: Tina Schrodel, Robert Prevedel, Karin Aumayr, Manuel Zimmer, Alipasha VaziriAbstract:Recent efforts in neuroscience research have been aimed at obtaining detailed anatomical neuronal wiring maps as well as information on how neurons in these networks engage in dynamic activities. Although the entire connectivity map of the nervous system of Caenorhabditis elegans has been known for more than 25 years, this knowledge has not been sufficient to predict all functional connections underlying behavior. To approach this goal, we developed a two-photon technique for brain-wide calcium imaging in C. elegans, using wide-field temporal focusing (WF-TeFo). Pivotal to our results was the use of a nuclear-localized, genetically encoded calcium indicator, NLS-GCaMP5K, that permits unambiguous discrimination of individual neurons within the densely packed head ganglia of C. elegans. We demonstrate near-simultaneous recording of activity of up to 70% of all head neurons. In combination with a Lab-on-a-Chip Device for stimulus delivery, this method provides an enabling platform for establishing functional maps of neuronal networks.
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Brain-wide 3D imaging of neuronal activity in Caenorhabditis elegans with sculpted light
Nature Methods, 2013Co-Authors: Tina Schrodel, Robert Prevedel, Karin Aumayr, Manuel Zimmer, Alipasha VaziriAbstract:This work describes wide-field temporal focusing, a two-photon volumetric imaging technique based on light sculpting that enables functional imaging of the majority of neurons in the head ganglia of C. elegans with high temporal and spatial resolution. Recent efforts in neuroscience research have been aimed at obtaining detailed anatomical neuronal wiring maps as well as information on how neurons in these networks engage in dynamic activities. Although the entire connectivity map of the nervous system of Caenorhabditis elegans has been known for more than 25 years, this knowledge has not been sufficient to predict all functional connections underlying behavior. To approach this goal, we developed a two-photon technique for brain-wide calcium imaging in C. elegans , using wide-field temporal focusing (WF-TeFo). Pivotal to our results was the use of a nuclear-localized, genetically encoded calcium indicator, NLS-GCaMP5K, that permits unambiguous discrimination of individual neurons within the densely packed head ganglia of C. elegans . We demonstrate near-simultaneous recording of activity of up to 70% of all head neurons. In combination with a Lab-on-a-Chip Device for stimulus delivery, this method provides an enabling platform for establishing functional maps of neuronal networks.
Robert Prevedel - One of the best experts on this subject based on the ideXlab platform.
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brain wide 3d imaging of neuronal activity in caenorhabditis elegans with sculpted light
Nature Methods, 2013Co-Authors: Tina Schrodel, Robert Prevedel, Karin Aumayr, Manuel Zimmer, Alipasha VaziriAbstract:Recent efforts in neuroscience research have been aimed at obtaining detailed anatomical neuronal wiring maps as well as information on how neurons in these networks engage in dynamic activities. Although the entire connectivity map of the nervous system of Caenorhabditis elegans has been known for more than 25 years, this knowledge has not been sufficient to predict all functional connections underlying behavior. To approach this goal, we developed a two-photon technique for brain-wide calcium imaging in C. elegans, using wide-field temporal focusing (WF-TeFo). Pivotal to our results was the use of a nuclear-localized, genetically encoded calcium indicator, NLS-GCaMP5K, that permits unambiguous discrimination of individual neurons within the densely packed head ganglia of C. elegans. We demonstrate near-simultaneous recording of activity of up to 70% of all head neurons. In combination with a Lab-on-a-Chip Device for stimulus delivery, this method provides an enabling platform for establishing functional maps of neuronal networks.
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Brain-wide 3D imaging of neuronal activity in Caenorhabditis elegans with sculpted light
Nature Methods, 2013Co-Authors: Tina Schrodel, Robert Prevedel, Karin Aumayr, Manuel Zimmer, Alipasha VaziriAbstract:This work describes wide-field temporal focusing, a two-photon volumetric imaging technique based on light sculpting that enables functional imaging of the majority of neurons in the head ganglia of C. elegans with high temporal and spatial resolution. Recent efforts in neuroscience research have been aimed at obtaining detailed anatomical neuronal wiring maps as well as information on how neurons in these networks engage in dynamic activities. Although the entire connectivity map of the nervous system of Caenorhabditis elegans has been known for more than 25 years, this knowledge has not been sufficient to predict all functional connections underlying behavior. To approach this goal, we developed a two-photon technique for brain-wide calcium imaging in C. elegans , using wide-field temporal focusing (WF-TeFo). Pivotal to our results was the use of a nuclear-localized, genetically encoded calcium indicator, NLS-GCaMP5K, that permits unambiguous discrimination of individual neurons within the densely packed head ganglia of C. elegans . We demonstrate near-simultaneous recording of activity of up to 70% of all head neurons. In combination with a Lab-on-a-Chip Device for stimulus delivery, this method provides an enabling platform for establishing functional maps of neuronal networks.
Jing Cheng - One of the best experts on this subject based on the ideXlab platform.
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integration of sperm motility and chemotaxis screening with a microchannel based Device
Chinese Journal of Laboratory Medicine, 2010Co-Authors: Lan Xie, Chao Han, Tian Qiu, Guoliang Huang, M A Rui, Jing ChengAbstract:Objective Sperm screening is an essential step in IVF procedures. The swim-up method, an assay on sperm motility, is used clinically to select the ideal sperm for subsequent manipulation. However, additional parameters, including acrosome reaction capability, chemotaxis, and thermotaxis are also important indicators of mammalian sperm health. To monitor both sperm motility and chemotaxis simultaneously during sperm screening, we designed and constructed a microDevice comprising a straight channel connected with a bi-branch channel that mimics the mammalian female reproductive tract. Methods The width and length of the straight channel were optimized to select the motile sperm. Cumulus cells were selectively cultured in the bi-branch channel to generate a chemoattractant-forming chemical gradient. Sperm chemotaxis was represented by the ratio of the sperm swimming towards different branches. Results The percentage of motile sperm was improved from ( 58. 5 ± 3. 8 ) % to ( 82. 6±2.9)% by a straight channel 7 mm in length and 1 mm in width. About 10% of sperm were found chemotactically responsive in our experiment, which is consistent with previous studies. Conclusion The combined evaluation of both sperm motility and chemotaxis was achieved for the first time, and the motile and chemotactically responsive sperm can be easily enriched on a Lab-on-a-Chip Device to improve IVF outcome. Key words: Sperm screening; Sperm health; Sperm motility; Chemotaxis; Microchannel
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integration of sperm motility and chemotaxis screening with a microchannel based Device
Clinical Chemistry, 2010Co-Authors: Lan Xie, Chao Han, Qiufang Zhang, Tian Qiu, Lei Wang, Guoliang Huang, Jie Qiao, Jundong Wang, Jing ChengAbstract:BACKGROUND: Sperm screening is an essential step in in vitro fertilization (IVF) procedures. The swim-up method, an assay for sperm motility, is used clinically to select the ideal sperm for subsequent manipulation. However, additional parameters, including acrosome reaction capability, chemotaxis, and thermotaxis, are also important indicators of mammalian sperm health. To monitor both sperm motility and chemotaxis simultaneously during sperm screening, we designed and constructed a microDevice comprising a straight channel connected with a bibranch channel that mimics the mammalian female reproductive tract. METHODS: The width and length of the straight channel were optimized to select the motile sperms. We selectively cultured cumulus cells in the bibranch channel to generate a chemoattractant-forming chemical gradient. Sperm chemotaxis was represented by the ratio of the sperm swimming toward different branches. RESULTS: The percentage of motile sperms improved from 58.5% (3.8%) to 82.6% (2.9%) by a straight channel 7 mm in length and 1 mm in width. About 10% of sperms were found to be chemotactically responsive in our experiment, which is consistent with previous studies. CONCLUSIONS: For the first time, we achieved the combined evaluation of both sperm motility and chemotaxis. The motile and chemotactically responsive sperms can easily be enriched on a Lab-on-a-Chip Device to improve IVF outcome.
Karin Aumayr - One of the best experts on this subject based on the ideXlab platform.
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brain wide 3d imaging of neuronal activity in caenorhabditis elegans with sculpted light
Nature Methods, 2013Co-Authors: Tina Schrodel, Robert Prevedel, Karin Aumayr, Manuel Zimmer, Alipasha VaziriAbstract:Recent efforts in neuroscience research have been aimed at obtaining detailed anatomical neuronal wiring maps as well as information on how neurons in these networks engage in dynamic activities. Although the entire connectivity map of the nervous system of Caenorhabditis elegans has been known for more than 25 years, this knowledge has not been sufficient to predict all functional connections underlying behavior. To approach this goal, we developed a two-photon technique for brain-wide calcium imaging in C. elegans, using wide-field temporal focusing (WF-TeFo). Pivotal to our results was the use of a nuclear-localized, genetically encoded calcium indicator, NLS-GCaMP5K, that permits unambiguous discrimination of individual neurons within the densely packed head ganglia of C. elegans. We demonstrate near-simultaneous recording of activity of up to 70% of all head neurons. In combination with a Lab-on-a-Chip Device for stimulus delivery, this method provides an enabling platform for establishing functional maps of neuronal networks.
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Brain-wide 3D imaging of neuronal activity in Caenorhabditis elegans with sculpted light
Nature Methods, 2013Co-Authors: Tina Schrodel, Robert Prevedel, Karin Aumayr, Manuel Zimmer, Alipasha VaziriAbstract:This work describes wide-field temporal focusing, a two-photon volumetric imaging technique based on light sculpting that enables functional imaging of the majority of neurons in the head ganglia of C. elegans with high temporal and spatial resolution. Recent efforts in neuroscience research have been aimed at obtaining detailed anatomical neuronal wiring maps as well as information on how neurons in these networks engage in dynamic activities. Although the entire connectivity map of the nervous system of Caenorhabditis elegans has been known for more than 25 years, this knowledge has not been sufficient to predict all functional connections underlying behavior. To approach this goal, we developed a two-photon technique for brain-wide calcium imaging in C. elegans , using wide-field temporal focusing (WF-TeFo). Pivotal to our results was the use of a nuclear-localized, genetically encoded calcium indicator, NLS-GCaMP5K, that permits unambiguous discrimination of individual neurons within the densely packed head ganglia of C. elegans . We demonstrate near-simultaneous recording of activity of up to 70% of all head neurons. In combination with a Lab-on-a-Chip Device for stimulus delivery, this method provides an enabling platform for establishing functional maps of neuronal networks.
Tina Schrodel - One of the best experts on this subject based on the ideXlab platform.
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brain wide 3d imaging of neuronal activity in caenorhabditis elegans with sculpted light
Nature Methods, 2013Co-Authors: Tina Schrodel, Robert Prevedel, Karin Aumayr, Manuel Zimmer, Alipasha VaziriAbstract:Recent efforts in neuroscience research have been aimed at obtaining detailed anatomical neuronal wiring maps as well as information on how neurons in these networks engage in dynamic activities. Although the entire connectivity map of the nervous system of Caenorhabditis elegans has been known for more than 25 years, this knowledge has not been sufficient to predict all functional connections underlying behavior. To approach this goal, we developed a two-photon technique for brain-wide calcium imaging in C. elegans, using wide-field temporal focusing (WF-TeFo). Pivotal to our results was the use of a nuclear-localized, genetically encoded calcium indicator, NLS-GCaMP5K, that permits unambiguous discrimination of individual neurons within the densely packed head ganglia of C. elegans. We demonstrate near-simultaneous recording of activity of up to 70% of all head neurons. In combination with a Lab-on-a-Chip Device for stimulus delivery, this method provides an enabling platform for establishing functional maps of neuronal networks.
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Brain-wide 3D imaging of neuronal activity in Caenorhabditis elegans with sculpted light
Nature Methods, 2013Co-Authors: Tina Schrodel, Robert Prevedel, Karin Aumayr, Manuel Zimmer, Alipasha VaziriAbstract:This work describes wide-field temporal focusing, a two-photon volumetric imaging technique based on light sculpting that enables functional imaging of the majority of neurons in the head ganglia of C. elegans with high temporal and spatial resolution. Recent efforts in neuroscience research have been aimed at obtaining detailed anatomical neuronal wiring maps as well as information on how neurons in these networks engage in dynamic activities. Although the entire connectivity map of the nervous system of Caenorhabditis elegans has been known for more than 25 years, this knowledge has not been sufficient to predict all functional connections underlying behavior. To approach this goal, we developed a two-photon technique for brain-wide calcium imaging in C. elegans , using wide-field temporal focusing (WF-TeFo). Pivotal to our results was the use of a nuclear-localized, genetically encoded calcium indicator, NLS-GCaMP5K, that permits unambiguous discrimination of individual neurons within the densely packed head ganglia of C. elegans . We demonstrate near-simultaneous recording of activity of up to 70% of all head neurons. In combination with a Lab-on-a-Chip Device for stimulus delivery, this method provides an enabling platform for establishing functional maps of neuronal networks.
