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Larry J Forney - One of the best experts on this subject based on the ideXlab platform.
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Influence of Lactobacillus crispatus, Lactobacillus Iners and Gardnerella vaginalis on bacterial vaginal composition in pregnant women
Archives of Gynecology and Obstetrics, 2021Co-Authors: Steven S. Witkin, Iara M Linhares, Antonio F. Moron, Larry J ForneyAbstract:Purpose To investigate associations between bacterial species in the vagina in mid-trimester pregnant women from Brazil. Methods The vaginal microbiome in 613 subjects was identified by analysis of the V1–V3 region of bacterial 16S ribosomal RNA and the relative prevalence of individual bacteria were determined. Results The bacterial species present in the greatest number of women were Lactobacillus crispatus (306 women) , L. Iners (298 women) and Gardnerella vaginalis (179 women). When present in the vagina, L. crispatus was the most abundant bacterium more than 85% of the time. In contrast, L. Iners and G. vaginalis were most abundant in 63% and 41% of women who were positive for these microorganisms, respectively ( p
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Influence of Lactobacillus crispatus, Lactobacillus Iners and Gardnerella vaginalis on bacterial vaginal composition in pregnant women.
Archives of gynecology and obstetrics, 2021Co-Authors: Steven S. Witkin, Iara M Linhares, Antonio F. Moron, Larry J ForneyAbstract:To investigate associations between bacterial species in the vagina in mid-trimester pregnant women from Brazil. The vaginal microbiome in 613 subjects was identified by analysis of the V1–V3 region of bacterial 16S ribosomal RNA and the relative prevalence of individual bacteria were determined. The bacterial species present in the greatest number of women were Lactobacillus crispatus (306 women), L. Iners (298 women) and Gardnerella vaginalis (179 women). When present in the vagina, L. crispatus was the most abundant bacterium more than 85% of the time. In contrast, L. Iners and G. vaginalis were most abundant in 63% and 41% of women who were positive for these microorganisms, respectively (p
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influence of Lactobacillus crispatus Lactobacillus Iners and gardnerella vaginalis on bacterial vaginal composition in pregnant women
Archives of Gynecology and Obstetrics, 2021Co-Authors: Steven S. Witkin, Iara M Linhares, Antonio F. Moron, Larry J ForneyAbstract:To investigate associations between bacterial species in the vagina in mid-trimester pregnant women from Brazil. The vaginal microbiome in 613 subjects was identified by analysis of the V1–V3 region of bacterial 16S ribosomal RNA and the relative prevalence of individual bacteria were determined. The bacterial species present in the greatest number of women were Lactobacillus crispatus (306 women), L. Iners (298 women) and Gardnerella vaginalis (179 women). When present in the vagina, L. crispatus was the most abundant bacterium more than 85% of the time. In contrast, L. Iners and G. vaginalis were most abundant in 63% and 41% of women who were positive for these microorganisms, respectively (p < 0.0001 vs. L. crispatus). The proportion of L. crispatus was negatively associated with the proportions of L. Iners, L. jensenii, L. gasseri, G. vaginalis, Megasphaera, Atopobium vaginae and Prevotella (p < 0.0001). In contrast, the proportion of G. vaginalis was positively associated with levels of Megasphaera, A. vaginae and Prevotella (p < 0.0001) while L. Iners proportion was unrelated to the proportion of L. jensenii, G. vaginalis, Megasphaera, A. vaginae or Prevotella. The composition of the vaginal microbiota in mid-trimester pregnant women is influenced by the relative concentrations of L. crispatus, L. Iners and G. vaginalis.
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Changes in the vaginal microbiota across a gradient of urbanization.
Scientific reports, 2020Co-Authors: Daniela Vargas-robles, Larry J Forney, Jacques Ravel, Natalia Morales, Iveth Rodríguez, Tahidid Nieves, Filipa Godoy-vitorino, Luis David Alcaraz, María-eglée Pérez, Maria Gloria Dominguez-belloAbstract:The vaginal microbiota of healthy women typically has low diversity, which increases after perturbations. Among these, lifestyle associated with certain sexual and antimicrobial practices may be associated with higher diversity. To test this hypothesis, we characterized the vaginal microbiota in the cervicovaginal and introital sites in sexually active Amerindians (N = 82) spanning urbanization, and in urban mestizos (N = 29), in the Venezuelan Amazonas. HPV status was also considered. Sampling was performed in an urban gradient from remote villages to a town, and women were individually classified by the degree of urbanization (low, medium, and high). Amerindian cervicovaginal and introital microbiota diversity were not associated with major changes in urbanization or ethnicity. There was a non-significant trend of increased diversity with urbanization, with a few taxa found overrepresented in urban Amerindians (Brevibacterium linens and Peptoniphilus lacrimalis) or mestizos (Mobiluncus mulieris and Prevotella sp.). Among all women, cervicovaginal and introital samples clustered, respectively, in four and two community state types (CSTs), where most profiles were dominated by Lactobacillus Iners, Gardnerella vaginalis or were highly diverse profiles. HPV status did not associate with microbial diversity. In conclusion, no association was found between urban level and the vaginal microbiome in Amerindian women, and little difference was found between ethnicities. L. Iners and high diversity profiles, associated with vaginal health outcomes, prevail in these populations.
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Nonoptimal Vaginal Microbiota After Azithromycin Treatment for Chlamydia trachomatis Infection.
The Journal of infectious diseases, 2019Co-Authors: Jeanne Tamarelle, Larry J Forney, Pawel Gajer, Michael S. Humphrys, Mishka Terplan, Katrina Mark, Anne C. M. Thiébaut, Rebecca M. Brotman, Elisabeth Delarocque-astagneauAbstract:We characterized the composition and structure of the vaginal microbiota in a cohort of 149 women with genital Chlamydia trachomatis infection at baseline who were followed quarterly for 9 months after antibiotic treatment. At time of diagnosis, the vaginal microbiota was dominated by Lactobacillus Iners or a diverse array of bacterial vaginosis-associated bacteria including Gardnerella vaginalis. Interestingly, L. Iners-dominated communities were most common after azithromycin treatment (1 g monodose), consistent with the observed relative resistance of L. Iners to azithromycin. Lactobacillus Iners-dominated communities have been associated with increased risk of C. trachomatis infection, suggesting that the impact of antibiotic treatment on the vaginal microbiota could favor reinfections. These results provide support for the dual need to account for the potential perturbing effect(s) of antibiotic treatment on the vaginal microbiota, and to develop strategies to protect and restore optimal vaginal microbiota.
Gregor Reid - One of the best experts on this subject based on the ideXlab platform.
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promising prebiotic candidate established by evaluation of lactitol lactulose raffinose and oligofructose for maintenance of a Lactobacillus dominated vaginal microbiota
Applied and Environmental Microbiology, 2017Co-Authors: Amy Mcmillan, Stephanie L Collins, Shannon Seney, Charlotte Van Der Veer, Remco Kort, Mark W Sumarah, Gregor ReidAbstract:Perturbations to the vaginal microbiota can lead to dysbiosis, including bacterial vaginosis (BV), which affects a large portion of the female population. In a healthy state, the vaginal microbiota is characterized by low diversity and colonization by Lactobacillus spp., whereas in BV, these species are displaced by a highly diverse population of bacteria associated with adverse vaginal health outcomes. Since prebiotic ingestion has been a highly effective approach to invigorate lactobacilli for improved intestinal health, we hypothesized that these compounds could stimulate lactobacilli at the expense of BV organisms to maintain vaginal health. Monocultures of commensal Lactobacillus crispatus, Lactobacillus vaginalis, Lactobacillus gasseri, Lactobacillus johnsonii, Lactobacillus jensenii, and Lactobacillus Iners, in addition to BV-associated organisms and Candida albicans, were tested for their ability to utilize a representative group of prebiotics consisting of lactitol, lactulose, raffinose, and oligofructose. The disaccharide lactulose was found to most broadly and specifically stimulate vaginal lactobacilli, including the strongly health-associated species L. crispatus, and importantly, not to stimulate BV organisms or C. albicans. Using freshly collected vaginal samples, we showed that exposure to lactulose promoted commensal Lactobacillus growth and dominance and resulted in healthy acidity partially through lactic acid production. This provides support for further testing of lactulose to prevent dysbiosis and potentially to reduce the need for antimicrobial agents in managing vaginal health. © 2018 American Society for Microbiology.
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Lactobacillus Iners : friend or foe?
Trends in microbiology, 2016Co-Authors: Mariya I. Petrova, Mario Vaneechoutte, Gregor Reid, Sarah LebeerAbstract:The vaginal microbial community is typically characterized by abundant lactobacilli. Lactobacillus Iners, a fairly recently detected species, is frequently present in the vaginal niche. However, the role of this species in vaginal health is unclear, since it can be detected in normal conditions as well as during vaginal dysbiosis, such as bacterial vaginosis, a condition characterized by an abnormal increase in bacterial diversity and lack of typical lactobacilli. Compared to other Lactobacillus species, L. Iners has more complex nutritional requirements and a Gram-variable morphology. L. Iners has an unusually small genome (ca. 1 Mbp), indicative of a symbiotic or parasitic lifestyle, in contrast to other lactobacilli that show niche flexibility and genomes of up to 3–4 Mbp. The presence of specific L. Iners genes, such as those encoding iron–sulfur proteins and unique σ-factors, reflects a high degree of niche specification. The genome of L. Iners strains also encodes inerolysin, a pore-forming toxin related to vaginolysin of Gardnerella vaginalis. Possibly, this organism may have clonal variants that in some cases promote a healthy vagina, and in other cases are associated with dysbiosis and disease. Future research should examine this friend or foe relationship with the host.
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Adhesion of Lactobacillus Iners AB-1 to Human Fibronectin: A Key Mediator for Persistence in the Vagina?
Reproductive Sciences, 2013Co-Authors: Amy Mcmillan, Jean M Macklaim, Jeremy P. Burton, Gregor ReidAbstract:Lactobacillus Iners is prominent in the human vagina and is able to persist despite development of bacterial vaginosis and treatment with antibiotics. A probable factor in its persistent survival is its ability to be retained in the vaginal epithelia. Genome sequencing of the strain showed an organism deplete of many metabolic pathways, yet equipped with fibronectin (Fn)-binding adhesins. The objective of the present study was to assess the ability of L Iners AB-1 to bind immobilized Fn. Results showed that the organism superiorly bound the protein compared to other species of Lactobacillus and known binders such as Staphylococcus aureus . Treatment of L Iners cells by protease rendered its binding abilities to Fn nonfunctional. The findings indicate a mechanism of vaginal persistence for a Lactobacillus species, with implications for reproductive health.
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comparative meta rna seq of the vaginal microbiota and differential expression by Lactobacillus Iners in health and dysbiosis
Microbiome, 2013Co-Authors: Jean M Macklaim, Andrew D Fernandes, Julia M Di Bella, Joanne Hammond, Gregor Reid, Gregory B GloorAbstract:Bacterial vaginosis (BV), the most common vaginal condition of reproductive-aged women, is associated with a highly diverse and heterogeneous microbiota. Here we present a proof-of-principle analysis to uncover the function of the microbiota using meta-RNA-seq to uncover genes and pathways that potentially differentiate healthy vaginal microbial communities from those in the dysbiotic state of bacterial vaginosis (BV). The predominant organism, Lactobacillus Iners, was present in both conditions and showed a differing expression profile in BV compared to healthy. Despite its minimal genome, L. Iners differentially expressed over 10% of its gene complement. Notably, in a BV environment L. Iners increased expression of a cholesterol-dependent cytolysin, and of mucin and glycerol transport and related metabolic enzymes. Genes belonging to a CRISPR system were greatly upregulated suggesting that bacteriophage influence the community. Reflective of L. Iners, the bacterial community as a whole demonstrated a preference for glycogen and glycerol as carbon sources under BV conditions. The predicted end-products of metabolism under BV conditions include an abundance of succinate and other short-chain fatty-acids, while healthy conditions are predicted to largely contain lactic acid. Our study underscores the importance of understanding the functional activity of the bacterial community in addition to characterizing the population structure when investigating the human microbiome.
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Comparative meta-RNA-seq of the vaginal microbiota and differential expression by Lactobacillus Iners in health and dysbiosis
Microbiome, 2013Co-Authors: Jean M Macklaim, Andrew D Fernandes, Julia M Di Bella, Joanne Hammond, Gregor Reid, Gregory B GloorAbstract:Background Bacterial vaginosis (BV), the most common vaginal condition of reproductive-aged women, is associated with a highly diverse and heterogeneous microbiota. Here we present a proof-of-principle analysis to uncover the function of the microbiota using meta-RNA-seq to uncover genes and pathways that potentially differentiate healthy vaginal microbial communities from those in the dysbiotic state of bacterial vaginosis (BV). Results The predominant organism, Lactobacillus Iners , was present in both conditions and showed a differing expression profile in BV compared to healthy. Despite its minimal genome, L. Iners differentially expressed over 10% of its gene complement. Notably, in a BV environment L. Iners increased expression of a cholesterol-dependent cytolysin, and of mucin and glycerol transport and related metabolic enzymes. Genes belonging to a CRISPR system were greatly upregulated suggesting that bacteriophage influence the community. Reflective of L. Iners , the bacterial community as a whole demonstrated a preference for glycogen and glycerol as carbon sources under BV conditions. The predicted end-products of metabolism under BV conditions include an abundance of succinate and other short-chain fatty-acids, while healthy conditions are predicted to largely contain lactic acid. Conclusions Our study underscores the importance of understanding the functional activity of the bacterial community in addition to characterizing the population structure when investigating the human microbiome.
Mario Vaneechoutte - One of the best experts on this subject based on the ideXlab platform.
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Lactobacillus Iners, the unusual suspect
Research in microbiology, 2017Co-Authors: Mario VaneechoutteAbstract:Lactobacillus Iners is an unusual Lactobacillus species which does not grow on de Man Rogosa Sharpe agar, does not produce d-lactic acid, and only limited amounts of hydrogen peroxide. Its production of inerolysin, a cytotoxin, is also unusual for a Lactobacillus. Epidemiological studies point to an ambiguous role for this species, which is quite often recovered in high numbers from vaginal dysbiosis and offers limited protection against vaginal dysbiosis and, subsequently, against sexually transmitted infections and adverse pregnancy outcome. Several data indicate that L. Iners might even contribute to the onset and maintenance of vaginal dysbiosis and be a risk factor for adverse pregnancy outcome.
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Lactobacillus Iners : friend or foe?
Trends in microbiology, 2016Co-Authors: Mariya I. Petrova, Mario Vaneechoutte, Gregor Reid, Sarah LebeerAbstract:The vaginal microbial community is typically characterized by abundant lactobacilli. Lactobacillus Iners, a fairly recently detected species, is frequently present in the vaginal niche. However, the role of this species in vaginal health is unclear, since it can be detected in normal conditions as well as during vaginal dysbiosis, such as bacterial vaginosis, a condition characterized by an abnormal increase in bacterial diversity and lack of typical lactobacilli. Compared to other Lactobacillus species, L. Iners has more complex nutritional requirements and a Gram-variable morphology. L. Iners has an unusually small genome (ca. 1 Mbp), indicative of a symbiotic or parasitic lifestyle, in contrast to other lactobacilli that show niche flexibility and genomes of up to 3–4 Mbp. The presence of specific L. Iners genes, such as those encoding iron–sulfur proteins and unique σ-factors, reflects a high degree of niche specification. The genome of L. Iners strains also encodes inerolysin, a pore-forming toxin related to vaginolysin of Gardnerella vaginalis. Possibly, this organism may have clonal variants that in some cases promote a healthy vagina, and in other cases are associated with dysbiosis and disease. Future research should examine this friend or foe relationship with the host.
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A DNA tool for early detection of vaginal dysbiosis in African women
Research in microbiology, 2015Co-Authors: Vicky Jespers, Mario Vaneechoutte, Tania Crucitti, Janneke Van De Wijgert, Sinead Delany-moretlwe, Mary Mwaura, Stephen Agabe, Joris MentenAbstract:A next-generation diagnostic tool for bacterial vaginosis, consisting of quantitative and/or qualitative molecular criteria, has not yet been identified. The optimal diagnostic tool should not only diagnose bacterial vaginosis in diverse populations, but should also detect early signs of transition to dysbiosis. We evaluated a tool based on log10-transformed qPCR data for Lactobacillus crispatus, Lactobacillus Iners, Lactobacillus jensenii, Lactobacillus gasseri, Lactobacillus vaginalis, Lactobacillus genus, Atopobium vaginae and Gardnerella vaginalis in vaginal specimens of 426 African women to detect dysbiosis and predict transition to dysbiosis. G. vaginalis (p = 0.204) and A. vaginae (p = 0.001) were more commonly present in women who evolved to an intermediate (Nugent 4-6) or bacterial vaginosis score (Nugent 7-10) compared to women who continued to have a normal Nugent score. The combination of G. vaginalis, A. vaginae and Lactobacillus genus counts performed best for diagnostic accuracy for bacterial vaginosis--sensitivity 93.4% and specificity 83.6%; and for predictive accuracy for bacterial vaginosis--sensitivity 79% and specificity 52%. L. crispatus combinations did not perform well. We conclude that a triple-G. vaginalis-A. vaginae-Lactobacillus genus-qPCR tool holds promise for research in sub-Saharan Africa or when developed as a next-generation clinical diagnostic modality for bacterial vaginosis, ideally engineered as a rapid assay.
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Antibiotic susceptibility of Atopobium vaginae
BMC infectious diseases, 2006Co-Authors: Ellen De Backer, Hans Verstraelen, Rita Verhelst, Geert Claeys, Gerda Verschraegen, Marleen Temmerman, Mario VaneechoutteAbstract:Previous studies have indicated that a recently described anaerobic bacterium, Atopobium vaginae is associated with bacterial vaginosis (BV). Thus far the four isolates of this fastidious micro-organism were found to be highly resistant to metronidazole and susceptible for clindamycin, two antibiotics preferred for the treatment of BV. Nine strains of Atopobium vaginae, four strains of Gardnerella vaginalis, two strains of Lactobacillus Iners and one strain each of Bifidobacterium breve, B. longum, L. crispatus, L. gasseri and L. jensenii were tested against 15 antimicrobial agents using the Etest. All nine strains of A. vaginae were highly resistant to nalidixic acid and colistin while being inhibited by low concentrations of clindamycin (range: 256 μg/ml) but susceptible to clindamycin (0.023 – 0.125 μg/ml). Clindamycin has higher activity against G. vaginalis and A. vaginae than metronidazole, but not all A. vaginae isolates are metronidazole resistant, as seemed to be a straightforward conclusion from previous studies on a more limited number of strains.
Urban Forsum - One of the best experts on this subject based on the ideXlab platform.
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Lactobacillus Iners a marker of changes in the vaginal flora
Journal of Clinical Microbiology, 2007Co-Authors: Tell Jakobsson, Urban ForsumAbstract:Lactobacillus Iners seems to be a species of lactobacilli occurring in the human vagina that deserves close scrutiny, as it was not found in earlier studies due to its peculiar culture requirements but is now discussed as one of the normal vaginal bacteria ([1][1], [4][2]). Following up on the
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Vaginal Lactobacillus flora of healthy Swedish women
Journal of Clinical Microbiology, 2002Co-Authors: Alejandra Vasquez, Tell Jakobsson, Urban Forsum, Siv Ahrne, Göran MolinAbstract:Species of the Lactobacillus acidophilus complex are generally considered to constitute most of the vaginal Lactobacillus flora, but the flora varies between studies. However, this may be due to difficulties in identifying the closely related species within the L. acidophilus complex by using traditional methods and to variations in the vaginal status of the participants. Two hundred two isolates from the vaginal fluids of 23 Swedish women without bacterial vaginosis, as defined by the criteria of Nugent et al. (R. P. Nugent, M. A. Krohn, and S. L. Hillier, J. Clin. Microbiol. 29:297-301, 1991), were typed by randomly amplified polymorphic DNA (RAPD) analysis and identified to the species level by temporal temperature gradient gel electrophoresis, multiplex PCR, and 16S ribosomal DNA sequencing. The vaginal flora of most participants was dominated by a single RAPD type, but five of them harbored two RAPD types representing two different species or strains. The most frequently occurring species were Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus Iners, and Lactobacillus jensenii. L. Iners has not previously been reported as one of the predominant Lactobacillus species in the vagina.
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Identification of randomly selected colonies of lactobacilli from normal vaginal fluid by pyrosequencing of the 16S rDNA variable V1 and V3 regions
APMIS : acta pathologica microbiologica et immunologica Scandinavica, 2002Co-Authors: Maria Tärnberg, Tell Jakobsson, Jon Jonasson, Urban ForsumAbstract:The present study aimed to characterize lactobacilli in vaginal fluid from 23 adult healthy women by using high-throughput DNA sequencing for identification of a large number of randomly selected colonies appearing on Rogosa and blood agar. The typing method was based on broad-range PCR of 16S rRNA gene variable regions V1 and V3, pyrosequencing, and classification of the fragments by alignment with NCBI-catalogued sequences and type strain sequences. Four major groups of sequences were found among the 402 isolates clearly corresponding to Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus Iners and Lactobacillus jensenii when compared to the sequences obtained for type strains. Our results indicate that pyrosequencing of 16S rRNA gene fragments as used here is a fast and reliable method well suited for identification to the species level, even within the Lactobacillus acidophilus complex.
Tell Jakobsson - One of the best experts on this subject based on the ideXlab platform.
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Lactobacillus Iners and the normal vaginal flora
2008Co-Authors: Tell JakobssonAbstract:The ecological niche of the vagina contains a large number of different microbes that are constantly interacting with each other and the host. Culture methods have not been sufficient in order to resolve the complexity of the normal vaginal flora. Further, the methods for delineating normal flora from not normal flora are not easily handled and are traditionally not based on culture but on microscopy of elements of the vaginal fluid. In the work presented in this thesis, an international collaboration was established that pin-pointed some of the difficulties in classifying vaginal floras, including staining, sampling, and discordance when lactobacilli are few in number, and that emphasized the importance of the size of the vision field in microscopes. As lactobacilli are prominent members of the normal vaginal flora they need to be carefully classified if further work towards more robust scoring tools is to be achieved.Phenotypic methods have not been able to separate the closely related Lactobacillus species of the vagina. Progress in molecular biology has provided possibilities to characterize these lactobacilli, which are mainly from the Lactobacillus acidophilus group. In this work a large number of strains collected by true random sampling were subjected to RAPD-PCR, TTGE and multiplex PCR for species identification. The major species found were L. crispatus, L. gasseri and L. jensenii and the recently described L. Iners. The presence of L. Iners has not been detected in previous studies due to its special nutrient requirements. Development of pyrosequencing technology also made it possible to match signatures of the two variable regions V1 and V3 of the 16S rRNA gene of the vaginal lactobacilli and identify them to the species level in a high throughput manner. The study confirmed that the dominating flora in women with normal vaginal flora comprises the four species mentioned previously. Repetitive sampling during IVF-treatment with highly varying oestrogen levels demonstrates changes that possibly occur during changes in the natural life cycle. Furthermore, L. Iners was found to be the first species to be established after spontaneously resolved or treated Bacterial Vaginosis.These findings can be of help in developing new strategies for regaining and retaining the normal vaginal flora.
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Lactobacillus Iners a marker of changes in the vaginal flora
Journal of Clinical Microbiology, 2007Co-Authors: Tell Jakobsson, Urban ForsumAbstract:Lactobacillus Iners seems to be a species of lactobacilli occurring in the human vagina that deserves close scrutiny, as it was not found in earlier studies due to its peculiar culture requirements but is now discussed as one of the normal vaginal bacteria ([1][1], [4][2]). Following up on the
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Vaginal Lactobacillus flora of healthy Swedish women
Journal of Clinical Microbiology, 2002Co-Authors: Alejandra Vasquez, Tell Jakobsson, Urban Forsum, Siv Ahrne, Göran MolinAbstract:Species of the Lactobacillus acidophilus complex are generally considered to constitute most of the vaginal Lactobacillus flora, but the flora varies between studies. However, this may be due to difficulties in identifying the closely related species within the L. acidophilus complex by using traditional methods and to variations in the vaginal status of the participants. Two hundred two isolates from the vaginal fluids of 23 Swedish women without bacterial vaginosis, as defined by the criteria of Nugent et al. (R. P. Nugent, M. A. Krohn, and S. L. Hillier, J. Clin. Microbiol. 29:297-301, 1991), were typed by randomly amplified polymorphic DNA (RAPD) analysis and identified to the species level by temporal temperature gradient gel electrophoresis, multiplex PCR, and 16S ribosomal DNA sequencing. The vaginal flora of most participants was dominated by a single RAPD type, but five of them harbored two RAPD types representing two different species or strains. The most frequently occurring species were Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus Iners, and Lactobacillus jensenii. L. Iners has not previously been reported as one of the predominant Lactobacillus species in the vagina.
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Identification of randomly selected colonies of lactobacilli from normal vaginal fluid by pyrosequencing of the 16S rDNA variable V1 and V3 regions
APMIS : acta pathologica microbiologica et immunologica Scandinavica, 2002Co-Authors: Maria Tärnberg, Tell Jakobsson, Jon Jonasson, Urban ForsumAbstract:The present study aimed to characterize lactobacilli in vaginal fluid from 23 adult healthy women by using high-throughput DNA sequencing for identification of a large number of randomly selected colonies appearing on Rogosa and blood agar. The typing method was based on broad-range PCR of 16S rRNA gene variable regions V1 and V3, pyrosequencing, and classification of the fragments by alignment with NCBI-catalogued sequences and type strain sequences. Four major groups of sequences were found among the 402 isolates clearly corresponding to Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus Iners and Lactobacillus jensenii when compared to the sequences obtained for type strains. Our results indicate that pyrosequencing of 16S rRNA gene fragments as used here is a fast and reliable method well suited for identification to the species level, even within the Lactobacillus acidophilus complex.
