The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Xiangyang Shi - One of the best experts on this subject based on the ideXlab platform.
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targeted ct mr dual mode imaging of human hepatocellular carcinoma using Lactobionic Acid modified polyethyleneimine entrapped gold nanoparticles
Journal of Materials Chemistry B, 2017Co-Authors: Jia Yang, Shihui Wen, Mingwu Shen, Linfeng Zheng, Guixiang Zhang, Xiangyang ShiAbstract:We report the synthesis and characterization of Lactobionic Acid-modified multifunctional polyethyleneimine-entrapped gold nanoparticles for targeted dual mode computed tomography/magnetic resonance imaging. The nanodevice displays good X-ray attenuation properties, good r1 relaxivity, and hepatocellular carcinoma targeting specificity, and can be used for targeted CT/MR imaging of hepatocellular carcinoma in vitro and in vivo.
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facile synthesis of Lactobionic Acid targeted iron oxide nanoparticles with ultrahigh relaxivity for targeted mr imaging of an orthotopic model of human hepatocellular carcinoma
Particle & Particle Systems Characterization, 2017Co-Authors: Ruizhi Wang, Xiangyang Shi, Ling Ding, Xiaolin Wang, Mingwu ShenAbstract:Development of multifunctional nanoprobes for tumor diagnosis is extremely important in the field of molecular imaging. In this study, the facile synthesis of Lactobionic Acid (LA)-targeted superparamagnetic iron oxide (Fe3O4) nanoparticles (NPs) with ultrahigh relaxivity for targeted magnetic resonance (MR) imaging of an orthotopic hepatocellular carcinoma (HCC) is reported. Polyethyleneimine (PEI)-stabilized Fe3O4 NPs prepared via a mild reduction route are sequentially coupled with fluorescein isothiocyanate and polyethylene glycol-LA (LA-PEG-COOH) segment, followed by acetylation of the remaining PEI surface amines. The formed LA-targeted Fe3O4 NPs are thoroughly characterized. It is shown that the developed multifunctional LA-targeted Fe3O4 NPs are colloidally stable and water-dispersible, display an ultrahigh r 2 relaxivity (579.89 × 10−3 m −1 s−1) and excellent hemocompatibility and cytocompatibility in the given concentration range, and can target HepG2 cells overexpressing asialoglycoprotein receptors as confirmed by in vitro cellular uptake assay, flow cytometry, and confocal microscopy. Most strikingly, the developed multifunctional LA-targeted Fe3O4 NPs can be used as a nanoprobe for targeted MR imaging of HepG2 cells in vitro and an orthotopic tumor model of HCC in vivo. With the ultrahigh r 2 relaxivity and the versatile PEI amine-mediated conjugation chemistry, a range of different Fe3O4 NP-based nanoprobes may be developed for theranostics of different types of cancer.
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capturing hepatocellular carcinoma cells using Lactobionic Acid functionalized electrospun polyvinyl alcohol polyethyleneimine nanofibers
RSC Advances, 2015Co-Authors: Yili Zhao, Mingwu Shen, Zhangyu Fan, Xiangyang ShiAbstract:We report a facile approach to immobilizing Lactobionic Acid (LA) onto electrospun polyvinyl alcohol (PVA)/polyethyleneimine (PEI) nanofibers through a polyethylene glycol (PEG) spacer for capturing hepatocellular carcinoma cells. In this work, electrospun PVA/PEI nanofibers were crosslinked using glutaraldehyde vapor, covalently conjugated with PEGylated LA via an N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) coupling reaction, followed by acetylation of the remaining PEI amines on the fiber surface. The formed LA-functionalized nanofibers were characterized via scanning electron microscopy and attenuated total reflectance-Fourier transform infrared spectroscopy. We show that the fiber morphology does not significantly change after fiber surface modification. The functionalized nanofibers display good hemocompatibility and superior capability to capture asialoglycoprotein receptor (ASGPR)-overexpressing hepatocellular carcinoma cells in vitro via a ligand–receptor interaction. The developed LA-modified PVA/PEI nanofibers may be applied to capture circulating tumor cells for cancer diagnosis applications.
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targeted doxorubicin delivery to hepatocarcinoma cells by Lactobionic Acid modified laponite nanodisks
New Journal of Chemistry, 2015Co-Authors: Guangxiang Chen, Xiangyang Shi, Xueyan Cao, Jianhua Wang, Rui GuoAbstract:In this study, we covalently conjugated polyethylene glycol-linked Lactobionic Acid (PEG-LA) onto the surface of laponite (LAP) nanodisks for the targeted delivery of doxorubicin (DOX) to liver cancer cells. LAP nanodisks were firstly modified with 3-aminopropyldimethylethoxysilane to introduce amino groups on the surface, and then PEG-LA were successfully conjugated to form targeted LM-PEG-LA nanodisks via EDC chemistry. Finally, the anticancer drug DOX was encapsulated into the synthesized nanocarriers with an exceptionally high loading efficiency of 91.5%. In vitro release studies showed that LM-PEG-LA/DOX could release drugs in a sustained manner with a higher speed under Acidic conditions than that under physiological ones. MTT assay results proved that LM-PEG-LA/DOX displayed a significant higher therapeutic efficacy in inhibiting the growth of hepatocellular carcinoma cells (HepG2 cells) than untargeted ones at the same DOX concentration. The targeting specificity of LM-PEG-LA/DOX was further demonstrated by flow cytometric analysis and confocal laser scanning microscopy. The developed LA-modified LAP nanodisks could serve as a targeted carrier for efficient loading and specific delivery of different anticancer drugs to liver cancer cells.
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targeted ct imaging of human hepatocellular carcinoma using low generation dendrimer entrapped gold nanoparticles modified with Lactobionic Acid
Journal of Materials Chemistry B, 2015Co-Authors: Yiyun Cao, Mingwu Shen, Hui Liu, Yu Luo, Jindong Xia, Xiangyang ShiAbstract:Development of cost-effective nanoscale contrast agents for targeted tumor computed tomography (CT) imaging remains a great challenge. Here, we report the synthesis of dendrimer-entrapped AuNPs (Au DENPs) using generation 2 poly(amidoamine) dendrimers pre-modified with fluorescein isothiocyanate via a thiourea linkage and Lactobionic Acid (LA) via a polyethylene glycol spacer as templates. The formed Au DENPs were characterized via different techniques and were used as a nanoprobe for targeted CT imaging of hepatocellular carcinoma (HCC). We show that the LA-modified Au DENPs with a mean Au core size of 1.8 nm are water-dispersible, colloidally stable under different temperatures (4–50 °C) and pH (5–8) conditions, and cytocompatible in the studied concentration range. Flow cytometry results reveal that the LA-Au DENPs are able to specifically target HepG2 cells (a human HCC cell line) overexpressing asialoglycoprotein receptors via a receptor-mediated targeting pathway. Importantly, the developed LA-Au DENPs can be used as a nanoprobe for targeted CT imaging of HepG2 cells in vitro and the xenografted tumor model in vivo. With the demonstrated organ compatibility, the developed LA-Au DENPs using low-generation dendrimers as templates can be a good candidate to be used as a highly efficient and cost-effective nanoprobe for targeted CT imaging of HCC.
Renzhong Qiao - One of the best experts on this subject based on the ideXlab platform.
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hepatocyte targeting and sustained release activity of water soluble anti hbv prodrug Lactobionic Acid grafted chitosan lamivudine conjugate
Reactive & Functional Polymers, 2016Co-Authors: Hongran Wang, Jin Zhang, Xinchao Yang, Renzhong QiaoAbstract:Abstract Lamivudine (3TC), a contemporary anti-HBV drug, has limited clinical application due to its non-specific biodistribution and side effects on normal cells. A new hepatocyte-targeting prodrug LA-CS-3TC is synthesized based on covalently linking 3TC and Lactobionic Acid with chitosan. Lactobionic Acid can specifically bind to the asialoglycoprotein receptor on the hepatocyte surface. In our study, the degrees of substitution of 3TC on chitosan polymer are 14.9%, while Lactobionic Acid moiety increases the intracellular accumulation of prodrug in HepG2.215 cells. LA-CS-3TC shows superior sustained release ability at pH 7.4 compared with CS-3TC and the major release product isopropyl-5′-O-lamivudine monophosphate (3TC-P-H) can enhance the therapeutic effects. Also, LA-CS-3TC demonstrates lower cytotoxicity and higher water-solubility than the parent compound. Hence, it is depicted that the chitosan modified with Lactobionic Acid could be applied as a potential anti-HBV drug vector with water-soluble, hepatocyte targeted, lowly cytotoxic and sustained release.
Mario Diaz - One of the best experts on this subject based on the ideXlab platform.
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bioactive synbiotic coatings with Lactobionic Acid and lactobacillus plantarum cect 9567 in the production and characterization of a new functional dairy product
Journal of Functional Foods, 2020Co-Authors: S Saezorviz, Manuel Rendueles, C Puertas, Ismael Marcet, Mario DiazAbstract:Abstract Lactobionic Acid (LBA), a prebiotic with beneficial health properties, can be mixed with a probiotic, Lactobacillus plantarum CECT 9567, to prepare novel synbiotic coatings. Coatings deposited on cottage cheese contained either probiotic (PRO, L. plantarum CECT 9567), prebiotic (PRE2 and PRE4; 20 and 40 g L−1 of LBA, respectively) or synbiotic compositions (SYN2 and SYN4). Coated cottage cheeses were analysed to determine probiotic and LBA concentration changes during storage, their textural properties and investigate the survival of bacteria during simulated digestion. Before the digestion test, PRO, SYN2 and SYN4 met minimal requirements to attain probiotic category and PRE2, PRE4, SYN2 and SYN4 contained adequate quantities of LBA throughout the experiment. Textural properties of cheese samples varied, with changes in the stickiness parameter. The digestion test showed that only SYN2 and SYN4 maintained acceptable probiotic values after simulated digestion, due to the presence of LBA, which increased probiotic survival.
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a new synbiotic dairy food containing Lactobionic Acid and lactobacillus casei
International Journal of Dairy Technology, 2019Co-Authors: Cristina Garcia, Manuel Rendueles, Lucia Bautista, Mario DiazAbstract:As well as its beneficial health properties, the incorporation of the prebiotic Lactobionic Acid (LBA) in fermented dairy products can provide a technological advantage due to its gelling capacities. This study aimed to develop a new functional dairy product containing LBA synthesised within the process itself by the bacterium Pseudomonas taetrolens. A probiotic Lactobacillus casei strain was introduced through a sequential fermentation system. After incubation, in the case of the most effective experimental procedure, a synbiotic fermented milk with 30 g/L of prebiotic LBA was obtained, together with an active L. casei population of 10⁹ cfu/mL and <1% lactose content.
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tunable decoupled overproduction of Lactobionic Acid in pseudomonas taetrolens through temperature control strategies
Process Biochemistry, 2017Co-Authors: Saul Alonso, Manuel Rendueles, Mario DiazAbstract:Abstract Triggering the switch from growth to Lactobionic Acid formation in Pseudomonas taetrolens is crucial to attain a high-yield bio-production system. This study elucidates how the growth-coupled and uncoupled Lactobionic Acid formation ratio can be modulated through the application of temperature-control strategies. Whereas the combination of high initial cell densities with a temperature value of 30 °C stimulated the onset of the Lactobionic Acid production phase from early cultivation times, temperature-stat conditions below 28 °C and above 30 °C led to a growth-coupled Lactobionic Acid formation with reduced cell growth. Further implementation of a temperature-control bioprocessing strategy at 28 °C with a pH-shift cultivation at 6.5 resulted in higher Lactobionic Acid yields (98%), volumetric productivity (2.04 g/L h), and specific productivity rates (1.73 g/g h) within only 24 h. Controlling the culture temperature at 28 °C via either temperature-stat or -shift bioprocessing strategies led to an overproduction of Lactobionic Acid, overcoming the low-yield mixed-growth-associated production patterns found at temperatures different than 28 °C. This novel temperature-driven approach provides a step forward in the bio-based production of Lactobionic Acid at high specific production rates and yields by uncoupling completely the production phase from the cell growth.
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bio production of Lactobionic Acid current status applications and future prospects
Biotechnology Advances, 2013Co-Authors: Saul Alonso, Manuel Rendueles, Mario DiazAbstract:Lactobionic Acid has appeared on the commercial scene as a versatile polyhydroxy Acid with numerous promising applications in the food, medicine, pharmaceutical, cosmetics and chemical industries. This high value-added bio-product has recently received growing attention as a bioactive compound, providing an excellent chemical platform for the synthesis of novel potentially biocompatible and biodegradable drug delivery vehicles. Recent advances in tissue engineering and nanomedicine have also underlined the increased importance of this organic Acid as a key biofunctionalization agent. The growing commercial relevance of Lactobionic Acid has therefore prompted the development of novel systems for its biotechnological production that are both sustainable and efficient. The present review explores recent advances and studies related to Lactobionic Acid bio-production, whether through microbial or enzymatic approaches, highlighting the key bioprocessing conditions for enhanced bio-production. Detailed overviews of the current microbial cell factories as well as downstream processing methodologies for Lactobionic Acid production are also presented. Furthermore, the potential prospects and current applications of this polyhydroxy Acid are also discussed, with an emphasis on the role of Lactobionic Acid as a key platform in the development of novel drugs, biomaterials, nanoparticles and biopolymer systems.
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selection method of ph conditions to establish pseudomonas taetrolens physiological states and Lactobionic Acid production
Applied Microbiology and Biotechnology, 2013Co-Authors: Saul Alonso, Manuel Rendueles, Mario DiazAbstract:Microbial physiological responses resulting from inappropriate bioprocessing conditions may have a marked impact on process performance within any fermentation system. The influence of different pH-control strategies on physiological status, microbial growth and Lactobionic Acid production from whey by Pseudomonas taetrolens during bioreactor cultivations has been investigated for the first time in this work. Both cellular behaviour and bioconversion efficiency from P. taetrolens were found to be negatively influenced by pH-control modes carried out at values lower than 6.0 and higher than 7.0. Production schemes were also influenced by the operational pH employed, with asynchronous production from damaged and metabolically active subpopulations at pH values lower than 6.0. Moreover, P. taetrolens showed reduced cellular proliferation and a subsequent delay in the onset of the production phase under Acidic conditions (pH < 6.0). Unlike cultivations performed at 6.5, both pH-shift and pH-stat cultivation strategies performed at pH values lower than 6.0 resulted in decreased Lactobionic Acid production. Whereas the cellular response showed a stress-induced physiological response under Acidic conditions, healthy functional cells were predominant at medium operational pH values (6.5–7.0). P. taetrolens thus displayed a robust physiological status at initial pH value of 6.5, resulting in an enhanced bioconversion yield and Lactobionic Acid productivity (7- and 4-fold higher compared to those attained at initial pH values of 4.5 and 5.0, respectively). These results have shown that pH-control modes strongly affected both the physiological response of cells and the biological performance of P. taetrolens, providing key information for bio-production of Lactobionic Acid on an industrial scale.
Chiawen Liu - One of the best experts on this subject based on the ideXlab platform.
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synthesis of Lactobionic Acid grafted pegylated chitosan with enhanced hepg2 cells transfection
Carbohydrate Polymers, 2011Co-Authors: Wenjen Lin, Tze Dan Chen, Chiawen Liu, Jiin Long Chen, Fu Hsiung ChangAbstract:The aim of this study was to develop a functional nanoparticulate carrier with an enhanced transfection in asialoglycoprotein receptor overexpressed HepG2 cells. A series of chemical modifications of chitosan were conducted by grafting a hydrophilic methoxy poly(ethylene glycol) (MPEG) and a receptor ligand, Lactobionic Acid (LA). The pegylation efficiency of deacetylated-depolymerized-chitosan (DADP-CS) was 26% (w/w). Increase in feed molar ratio of Lactobionic Acid from 0.25 to 0.5 increased LA grafting degree of Lactobionic Acid-grafted-pegylated-chitosan (DADP-CS-(O-MPEG)-(N-LA)) from 10% to 28%. Plasmid DNA was more compacted by DADP-CS-(O-MPEG)-(N-LA) than by pegylated-chitosan (DADP-CS-(O-MPEG)) in terms of smaller particle size of DADP-CS-(O-MPEG)-(N-LA)/DNA nanoparticle complex. The bearing of receptor ligand of DADP-CS-(O-MPEG)-(N-LA) (45.3%) showed better transfection efficiency than ligand-free DADP-CS-(O-MPEG) (19.8%) in asialoglycoprotein receptor overexpressed HepG2 cells.
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synthesis and characterization of Lactobionic Acid grafted pegylated chitosan and nanoparticle complex application
Polymer, 2009Co-Authors: Wenjen Lin, Tze Dan Chen, Chiawen LiuAbstract:Abstract A series of chemical modifications of chitosan were conducted by grafting a hydrophilic methoxy poly(ethylene glycol) (MPEG) and a target sugar molecule Lactobionic Acid (LA). The MPEG was grafted onto C6–OH position of chitosan, and the grafting degree was reduced for chitosan with high degree of depolymerization. The Lactobionic Acid was proposed to graft onto C2–NH2 position of chitosan. The LA grafting ratio was dependent on pegylation degree of chitosan, where the flexibility and shielding effect of MPEG hindered LA grafting onto chitosan. The Lactobionic Acid grafted pegylated chitosan, DADP-CS-(O-MPEG)-(N-LA), successfully provoked DNA condensation into nanoparticle complexes due to electrostatic compaction. The presence of MPEG on DADP-CS-(O-MPEG)-(N-LA) played an important role on preventing nanoparticle aggregation.
Mingwu Shen - One of the best experts on this subject based on the ideXlab platform.
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targeted ct mr dual mode imaging of human hepatocellular carcinoma using Lactobionic Acid modified polyethyleneimine entrapped gold nanoparticles
Journal of Materials Chemistry B, 2017Co-Authors: Jia Yang, Shihui Wen, Mingwu Shen, Linfeng Zheng, Guixiang Zhang, Xiangyang ShiAbstract:We report the synthesis and characterization of Lactobionic Acid-modified multifunctional polyethyleneimine-entrapped gold nanoparticles for targeted dual mode computed tomography/magnetic resonance imaging. The nanodevice displays good X-ray attenuation properties, good r1 relaxivity, and hepatocellular carcinoma targeting specificity, and can be used for targeted CT/MR imaging of hepatocellular carcinoma in vitro and in vivo.
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facile synthesis of Lactobionic Acid targeted iron oxide nanoparticles with ultrahigh relaxivity for targeted mr imaging of an orthotopic model of human hepatocellular carcinoma
Particle & Particle Systems Characterization, 2017Co-Authors: Ruizhi Wang, Xiangyang Shi, Ling Ding, Xiaolin Wang, Mingwu ShenAbstract:Development of multifunctional nanoprobes for tumor diagnosis is extremely important in the field of molecular imaging. In this study, the facile synthesis of Lactobionic Acid (LA)-targeted superparamagnetic iron oxide (Fe3O4) nanoparticles (NPs) with ultrahigh relaxivity for targeted magnetic resonance (MR) imaging of an orthotopic hepatocellular carcinoma (HCC) is reported. Polyethyleneimine (PEI)-stabilized Fe3O4 NPs prepared via a mild reduction route are sequentially coupled with fluorescein isothiocyanate and polyethylene glycol-LA (LA-PEG-COOH) segment, followed by acetylation of the remaining PEI surface amines. The formed LA-targeted Fe3O4 NPs are thoroughly characterized. It is shown that the developed multifunctional LA-targeted Fe3O4 NPs are colloidally stable and water-dispersible, display an ultrahigh r 2 relaxivity (579.89 × 10−3 m −1 s−1) and excellent hemocompatibility and cytocompatibility in the given concentration range, and can target HepG2 cells overexpressing asialoglycoprotein receptors as confirmed by in vitro cellular uptake assay, flow cytometry, and confocal microscopy. Most strikingly, the developed multifunctional LA-targeted Fe3O4 NPs can be used as a nanoprobe for targeted MR imaging of HepG2 cells in vitro and an orthotopic tumor model of HCC in vivo. With the ultrahigh r 2 relaxivity and the versatile PEI amine-mediated conjugation chemistry, a range of different Fe3O4 NP-based nanoprobes may be developed for theranostics of different types of cancer.
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capturing hepatocellular carcinoma cells using Lactobionic Acid functionalized electrospun polyvinyl alcohol polyethyleneimine nanofibers
RSC Advances, 2015Co-Authors: Yili Zhao, Mingwu Shen, Zhangyu Fan, Xiangyang ShiAbstract:We report a facile approach to immobilizing Lactobionic Acid (LA) onto electrospun polyvinyl alcohol (PVA)/polyethyleneimine (PEI) nanofibers through a polyethylene glycol (PEG) spacer for capturing hepatocellular carcinoma cells. In this work, electrospun PVA/PEI nanofibers were crosslinked using glutaraldehyde vapor, covalently conjugated with PEGylated LA via an N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) coupling reaction, followed by acetylation of the remaining PEI amines on the fiber surface. The formed LA-functionalized nanofibers were characterized via scanning electron microscopy and attenuated total reflectance-Fourier transform infrared spectroscopy. We show that the fiber morphology does not significantly change after fiber surface modification. The functionalized nanofibers display good hemocompatibility and superior capability to capture asialoglycoprotein receptor (ASGPR)-overexpressing hepatocellular carcinoma cells in vitro via a ligand–receptor interaction. The developed LA-modified PVA/PEI nanofibers may be applied to capture circulating tumor cells for cancer diagnosis applications.
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targeted ct imaging of human hepatocellular carcinoma using low generation dendrimer entrapped gold nanoparticles modified with Lactobionic Acid
Journal of Materials Chemistry B, 2015Co-Authors: Yiyun Cao, Mingwu Shen, Hui Liu, Yu Luo, Jindong Xia, Xiangyang ShiAbstract:Development of cost-effective nanoscale contrast agents for targeted tumor computed tomography (CT) imaging remains a great challenge. Here, we report the synthesis of dendrimer-entrapped AuNPs (Au DENPs) using generation 2 poly(amidoamine) dendrimers pre-modified with fluorescein isothiocyanate via a thiourea linkage and Lactobionic Acid (LA) via a polyethylene glycol spacer as templates. The formed Au DENPs were characterized via different techniques and were used as a nanoprobe for targeted CT imaging of hepatocellular carcinoma (HCC). We show that the LA-modified Au DENPs with a mean Au core size of 1.8 nm are water-dispersible, colloidally stable under different temperatures (4–50 °C) and pH (5–8) conditions, and cytocompatible in the studied concentration range. Flow cytometry results reveal that the LA-Au DENPs are able to specifically target HepG2 cells (a human HCC cell line) overexpressing asialoglycoprotein receptors via a receptor-mediated targeting pathway. Importantly, the developed LA-Au DENPs can be used as a nanoprobe for targeted CT imaging of HepG2 cells in vitro and the xenografted tumor model in vivo. With the demonstrated organ compatibility, the developed LA-Au DENPs using low-generation dendrimers as templates can be a good candidate to be used as a highly efficient and cost-effective nanoprobe for targeted CT imaging of HCC.
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multifunctional Lactobionic Acid modified dendrimers for targeted drug delivery to liver cancer cells investigating the role played by peg spacer
ACS Applied Materials & Interfaces, 2014Co-Authors: Jingyi Zhu, Shihui Wen, Mingwu Shen, Xiangyang ShiAbstract:We report the development of a Lactobionic Acid (LA)-modified multifunctional dendrimer-based carrier system for targeted therapy of liver cancer cells overexpressing asialoglycoprotein receptors. In this study, generation 5 (G5) poly(amidoamine) (PAMAM) dendrimers were sequentially modified with fluorescein isothiocyanate (FI) and LA (or polyethylene glycol (PEG)-linked LA, PEG-LA), followed by acetylation of the remaining dendrimer terminal amines. The synthesized G5.NHAc-FI-LA or G5.NHAc-FI-PEG-LA conjugates (NHAc denotes acetamide groups) were used to encapsulate a model anticancer drug doxorubicin (DOX). We show that both conjugates are able to encapsulate approximately 5.0 DOX molecules within each dendrimer and the formed dendrimer/DOX complexes are stable under different pH conditions and different aqueous media. The G5.NHAc-FI-PEG-LA conjugate appears to have a better cytocompatibility, enables a slightly faster DOX release rate, and displays better liver cancer cell targeting ability than the G5...
