The Experts below are selected from a list of 276 Experts worldwide ranked by ideXlab platform
Ilya Raskin - One of the best experts on this subject based on the ideXlab platform.
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A rapid and efficient in vitro regeneration system for lettuce (Lactuca sativa L.)
Plant Methods, 2017Co-Authors: Isabel Armas, Natalia Pogrebnyak, Ilya RaskinAbstract:Successful biotechnological improvement of crop plants requires a reliable and efficient in vitro regeneration system. Lettuce (Lactuca sativa L.), one the most important vegetable crops worldwide, is strongly genotype-dependent in terms of regeneration capacity, limiting the potential for biotechnological improvement of cultivars which show recalcitrance under currently available protocols. The effect of different nutrient sources, plant hormone combinations and activated charcoal supplementation on shoot induction efficiency was evaluated on the cultivar `RSL NFR', which had previously shown poor regeneration efficiency.
Ken Ichi Tomizawa - One of the best experts on this subject based on the ideXlab platform.
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efficient and stable transformation of Lactuca sativa l cv cisco lettuce plastids
Transgenic Research, 2006Co-Authors: Hirosuke Kanamoto, Atsushi Yamashita, Hiroshi Asao, Satoru Okumura, Hisabumi Takase, Masahira Hattori, Akiho Yokota, Ken Ichi TomizawaAbstract:Transgenic plastids offer unique advantages in plant biotechnology, including high-level foreign protein expression. However, broad application of plastid genome engineering in biotechnology has been largely hampered by the lack of plastid transformation systems for major crops. Here we describe the development of a plastid transformation system for lettuce, Lactuca sativa L. cv. Cisco. The transforming DNA carries a spectinomycin-resistance gene (aadA) under the control of lettuce chloroplast regulatory expression elements, flanked by two adjacent lettuce plastid genome sequences allowing its targeted insertion between the rbcL and accD genes. On average, we obtained 1 transplastomic lettuce plant per bombardment. We show that lettuce leaf chloroplasts can express transgene-encoded GFP to ~36% of the total soluble protein. All transplastomic T0 plants were fertile and the T1 progeny uniformly showed stability of the transgene in the chloroplast genome. This system will open up new possibilities for the efficient production of edible vaccines, pharmaceuticals, and antibodies in plants.
Isabel Armas - One of the best experts on this subject based on the ideXlab platform.
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A rapid and efficient in vitro regeneration system for lettuce (Lactuca sativa L.)
Plant Methods, 2017Co-Authors: Isabel Armas, Natalia Pogrebnyak, Ilya RaskinAbstract:Successful biotechnological improvement of crop plants requires a reliable and efficient in vitro regeneration system. Lettuce (Lactuca sativa L.), one the most important vegetable crops worldwide, is strongly genotype-dependent in terms of regeneration capacity, limiting the potential for biotechnological improvement of cultivars which show recalcitrance under currently available protocols. The effect of different nutrient sources, plant hormone combinations and activated charcoal supplementation on shoot induction efficiency was evaluated on the cultivar `RSL NFR', which had previously shown poor regeneration efficiency.
Hirosuke Kanamoto - One of the best experts on this subject based on the ideXlab platform.
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efficient and stable transformation of Lactuca sativa l cv cisco lettuce plastids
Transgenic Research, 2006Co-Authors: Hirosuke Kanamoto, Atsushi Yamashita, Hiroshi Asao, Satoru Okumura, Hisabumi Takase, Masahira Hattori, Akiho Yokota, Ken Ichi TomizawaAbstract:Transgenic plastids offer unique advantages in plant biotechnology, including high-level foreign protein expression. However, broad application of plastid genome engineering in biotechnology has been largely hampered by the lack of plastid transformation systems for major crops. Here we describe the development of a plastid transformation system for lettuce, Lactuca sativa L. cv. Cisco. The transforming DNA carries a spectinomycin-resistance gene (aadA) under the control of lettuce chloroplast regulatory expression elements, flanked by two adjacent lettuce plastid genome sequences allowing its targeted insertion between the rbcL and accD genes. On average, we obtained 1 transplastomic lettuce plant per bombardment. We show that lettuce leaf chloroplasts can express transgene-encoded GFP to ~36% of the total soluble protein. All transplastomic T0 plants were fertile and the T1 progeny uniformly showed stability of the transgene in the chloroplast genome. This system will open up new possibilities for the efficient production of edible vaccines, pharmaceuticals, and antibodies in plants.
Natalia Pogrebnyak - One of the best experts on this subject based on the ideXlab platform.
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A rapid and efficient in vitro regeneration system for lettuce (Lactuca sativa L.)
Plant Methods, 2017Co-Authors: Isabel Armas, Natalia Pogrebnyak, Ilya RaskinAbstract:Successful biotechnological improvement of crop plants requires a reliable and efficient in vitro regeneration system. Lettuce (Lactuca sativa L.), one the most important vegetable crops worldwide, is strongly genotype-dependent in terms of regeneration capacity, limiting the potential for biotechnological improvement of cultivars which show recalcitrance under currently available protocols. The effect of different nutrient sources, plant hormone combinations and activated charcoal supplementation on shoot induction efficiency was evaluated on the cultivar `RSL NFR', which had previously shown poor regeneration efficiency.
