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Mudassir Khan - One of the best experts on this subject based on the ideXlab platform.
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production of nitrogen fixing azotobacter sr 4 and phosphorus solubilizing aspergillus niger and their evaluation on Lagenaria siceraria and abelmoschus esculentus
Biotechnology Reports, 2019Co-Authors: Misbahud Din, Rubina Nelofer, Muhammad Salman, Faisal Hayat Khan, Asad Khan, Munib Ahmad, Fazal Jalil, Jalal Ud Din, Mudassir KhanAbstract:Abstract Aims Current study was aimed to produce nitrogen fixing Azotobacter sp. (SR-4) and phosphorus solubilizers Aspergillus niger (A. niger) and to evaluate their efficiency as biofertilizers for agricultural practices. Methods Two biofertilizer including nitrogen fixing and phosphorus solubilizing were grown. The nitrogen fixing efficiency of Azotobacter (SR-4) was determined by Kjeldahl method. Similarly, Vanadomoybdate method was used to measure the soluble phosphorus while Heinonen method was used to analyze concentration of phytase and phosphatase in the cultures. Furthermore, both biofertilizers were tested in a field trail on Lagenaria siceraria (bottle gourd) and Abelmoschus esculentus (okra). Results The Azotobacter (SR-4) strain was found efficient nitrogen fixer as 35.08 mg of nitrogen per gram of carbon was produced after 72 h of fermentation. Similarly, A. niger strain excrete extracellular phosphate solubilizing enzymes such as phytase (133UI in 48 h of fermentation) and phosphatase (170UI in 48 h of fermentation) which can solubilize the rock phosphate and make it available to plants. In field trials on selected plants (L. siceraria and A. esculentus), both biofertilizers showed significant increase in plant height, leaf length/width, fruit size and number of fruits per plant when compared with controls/untreated plants. Furthermore, plants co-inoculated with both the N fixing Azotobacter and phosphorus solubilizing A. niger have enhanced performance than those treated with each biofertilizer alone. Conclusion The inoculation of seeds with A. niger and Azotobacter may replace costly and environment toxic chemical fertilizers with environment friendly and cost effective biofertilizers.
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Production of nitrogen fixing Azotobacter (SR-4) and phosphorus solubilizing Aspergillus niger and their evaluation on Lagenaria siceraria and Abelmoschus esculentus
'Elsevier BV', 2019Co-Authors: Misbahud Din, Rubina Nelofer, Muhammad Salman, Faisal Hayat Khan, Asad Khan, Munib Ahmad, Fazal Jalil, Jalal Ud Din, Mudassir KhanAbstract:Aims: Current study was aimed to produce nitrogen fixing Azotobacter sp. (SR-4) and phosphorus solubilizers Aspergillus niger (A. niger) and to evaluate their efficiency as biofertilizers for agricultural practices. Methods: Two biofertilizer including nitrogen fixing and phosphorus solubilizing were grown. The nitrogen fixing efficiency of Azotobacter (SR-4) was determined by Kjeldahl method. Similarly, Vanadomoybdate method was used to measure the soluble phosphorus while Heinonen method was used to analyze concentration of phytase and phosphatase in the cultures. Furthermore, both biofertilizers were tested in a field trail on Lagenaria siceraria (bottle gourd) and Abelmoschus esculentus (okra). Results: The Azotobacter (SR-4) strain was found efficient nitrogen fixer as 35.08 mg of nitrogen per gram of carbon was produced after 72 h of fermentation. Similarly, A. niger strain excrete extracellular phosphate solubilizing enzymes such as phytase (133UI in 48 h of fermentation) and phosphatase (170UI in 48 h of fermentation) which can solubilize the rock phosphate and make it available to plants. In field trials on selected plants (L. siceraria and A. esculentus), both biofertilizers showed significant increase in plant height, leaf length/width, fruit size and number of fruits per plant when compared with controls/untreated plants. Furthermore, plants co-inoculated with both the N fixing Azotobacter and phosphorus solubilizing A. niger have enhanced performance than those treated with each biofertilizer alone. Conclusion: The inoculation of seeds with A. niger and Azotobacter may replace costly and environment toxic chemical fertilizers with environment friendly and cost effective biofertilizers. Keywords: Biofertilizers, Azotobacter, A. niger, Bottle gourd and okr
Misbahud Din - One of the best experts on this subject based on the ideXlab platform.
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production of nitrogen fixing azotobacter sr 4 and phosphorus solubilizing aspergillus niger and their evaluation on Lagenaria siceraria and abelmoschus esculentus
Biotechnology Reports, 2019Co-Authors: Misbahud Din, Rubina Nelofer, Muhammad Salman, Faisal Hayat Khan, Asad Khan, Munib Ahmad, Fazal Jalil, Jalal Ud Din, Mudassir KhanAbstract:Abstract Aims Current study was aimed to produce nitrogen fixing Azotobacter sp. (SR-4) and phosphorus solubilizers Aspergillus niger (A. niger) and to evaluate their efficiency as biofertilizers for agricultural practices. Methods Two biofertilizer including nitrogen fixing and phosphorus solubilizing were grown. The nitrogen fixing efficiency of Azotobacter (SR-4) was determined by Kjeldahl method. Similarly, Vanadomoybdate method was used to measure the soluble phosphorus while Heinonen method was used to analyze concentration of phytase and phosphatase in the cultures. Furthermore, both biofertilizers were tested in a field trail on Lagenaria siceraria (bottle gourd) and Abelmoschus esculentus (okra). Results The Azotobacter (SR-4) strain was found efficient nitrogen fixer as 35.08 mg of nitrogen per gram of carbon was produced after 72 h of fermentation. Similarly, A. niger strain excrete extracellular phosphate solubilizing enzymes such as phytase (133UI in 48 h of fermentation) and phosphatase (170UI in 48 h of fermentation) which can solubilize the rock phosphate and make it available to plants. In field trials on selected plants (L. siceraria and A. esculentus), both biofertilizers showed significant increase in plant height, leaf length/width, fruit size and number of fruits per plant when compared with controls/untreated plants. Furthermore, plants co-inoculated with both the N fixing Azotobacter and phosphorus solubilizing A. niger have enhanced performance than those treated with each biofertilizer alone. Conclusion The inoculation of seeds with A. niger and Azotobacter may replace costly and environment toxic chemical fertilizers with environment friendly and cost effective biofertilizers.
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Production of nitrogen fixing Azotobacter (SR-4) and phosphorus solubilizing Aspergillus niger and their evaluation on Lagenaria siceraria and Abelmoschus esculentus
'Elsevier BV', 2019Co-Authors: Misbahud Din, Rubina Nelofer, Muhammad Salman, Faisal Hayat Khan, Asad Khan, Munib Ahmad, Fazal Jalil, Jalal Ud Din, Mudassir KhanAbstract:Aims: Current study was aimed to produce nitrogen fixing Azotobacter sp. (SR-4) and phosphorus solubilizers Aspergillus niger (A. niger) and to evaluate their efficiency as biofertilizers for agricultural practices. Methods: Two biofertilizer including nitrogen fixing and phosphorus solubilizing were grown. The nitrogen fixing efficiency of Azotobacter (SR-4) was determined by Kjeldahl method. Similarly, Vanadomoybdate method was used to measure the soluble phosphorus while Heinonen method was used to analyze concentration of phytase and phosphatase in the cultures. Furthermore, both biofertilizers were tested in a field trail on Lagenaria siceraria (bottle gourd) and Abelmoschus esculentus (okra). Results: The Azotobacter (SR-4) strain was found efficient nitrogen fixer as 35.08 mg of nitrogen per gram of carbon was produced after 72 h of fermentation. Similarly, A. niger strain excrete extracellular phosphate solubilizing enzymes such as phytase (133UI in 48 h of fermentation) and phosphatase (170UI in 48 h of fermentation) which can solubilize the rock phosphate and make it available to plants. In field trials on selected plants (L. siceraria and A. esculentus), both biofertilizers showed significant increase in plant height, leaf length/width, fruit size and number of fruits per plant when compared with controls/untreated plants. Furthermore, plants co-inoculated with both the N fixing Azotobacter and phosphorus solubilizing A. niger have enhanced performance than those treated with each biofertilizer alone. Conclusion: The inoculation of seeds with A. niger and Azotobacter may replace costly and environment toxic chemical fertilizers with environment friendly and cost effective biofertilizers. Keywords: Biofertilizers, Azotobacter, A. niger, Bottle gourd and okr
P G Yeole - One of the best experts on this subject based on the ideXlab platform.
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antihyperlipidemic effect of the methanolic extract from Lagenaria siceraria stand fruit in hyperlipidemic rats
Journal of Ethnopharmacology, 2009Co-Authors: B V Ghule, Mahavir H. Ghante, A N Saoji, P G YeoleAbstract:Ethnopharmacological relevance Lagenaria siceraria Stand. (Cucurbitaceae) fruits have been used, traditionally, in the treatment of hyperlipidemia and atherosclerotic impasse and considered as cardioprotective and cardiotonic drug. Aim of the study The present work was undertaken to investigate the effects of methanolic extract from Lagenaria siceraria in experimentally induced hyperlipidemia in rats. Materials and methods Methanolic extract of Lagenaria siceraria fruits (LSFE) (100, 200 and 300 mg/kg; p.o.) was administered to the high fat-diet-induced hyperlipidemic rats for 30 days to evaluate its antihyperlipidemic activity. Atorvastatin (10 mg/kg; p.o.) was used as a standard drug. Results At the 30th day, most significant reduction in lipid levels in the LSFE treated rats as compared to the rats fed with high-fat diet at the 0th day were: total cholesterol 290.14 ± 18.42 mg/dl vs. 228.58 ± 16.38 mg/dl, low-density lipoprotein cholesterol 195.14 ± 8.86 mg/dl vs. 120.57 ± 8.11 mg/dl, triglyceride 232.41 ± 15.22 mg/dl vs. 181.79 ± 15.68 mg/dl, very low-density lipoprotein cholesterol 46.48 ± 3.04 mg/dl vs. 36.35 ± 3.13 mg/dl (P < 0.0001). Conversely, high-density lipoprotein cholesterol levels were significantly (P < 0.0001) increased from 48.52 ± 6.52 to 71.66 ± 5.14 mg/dl. The increase in weight in rats administered with LSFE was less when compared to rats fed with high-fat diet. Moreover, LSFE also exhibited significant increase in excretion of bile acids. Conclusion The results demonstrate that the LSFE has a definite antihyperlipidemic potential. There is also a valid scientific basis for consuming it in the treatment of coronary artery diseases in India.
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diuretic activity of Lagenaria siceraria fruit extracts in rats
Indian Journal of Pharmaceutical Sciences, 2007Co-Authors: B V Ghule, Mahavir H. Ghante, P G Yeole, A N SaojiAbstract:Vacuum dried juice extract and methanol extract of the fruits of Lagenaria siceraria Mol. have been evaluated for its diuretic activity in albino rats. Different parameters viz. total urine volume (corrected for water intake during the test period), urine concentration of electrolytes such as sodium, potassium and chloride have been evaluated. The rats treated with vacuum dried Lagenaria siceraria juice extract (LSJE) and Lagenaria siceraria methanol extract (LSME) (100-200 mg/kg; p.o. ) showed higher urine volume when compared to the respective control. Both LSJE and LSME have exhibited dose-dependent increase in the excretion of electrolytes when compared to control group. The elevated diuretic potential of LSFE and LSME was statistically significant ( P <0.05) and comparable to that of the standard diuretic agent furosemide (20 mg/kg; i.p.).
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hypolipidemic and antihyperlipidemic effects of Lagenaria siceraria mol fruit extracts
Indian Journal of Experimental Biology, 2006Co-Authors: B V Ghule, Mahavir H. Ghante, A N Saoji, P G YeoleAbstract:Bottle gourd [(Lagenaria siceraria (Mol.) Stand.] fruit is ascribed with many therapeutic effects. The present study was undertaken to explore the antihyperlipidemic effect of four different extracts viz. petroleum ether, chloroform, alcoholic and aqueous extracts from bottle gourd in Triton-induced hyperlipidemic rats and their hypolipidemic effects in normocholesteremic rats. The study is comprised preliminary phytochemical screening of the extracts. Oral administration of the extracts, at doses of 200 and 400 mg/kg body weight in rats, dose-dependently inhibited the total cholesterol, triglycerides, low-density lipoproteins level, and significantly increased the high density lipoproteins level. However, petroleum ether extract did not show the significant effects. Both the chloroform and alcoholic extract exhibited more significant effects in lowering total cholesterol, triglycerides and low density lipoproteins along with increase in HDL as compared to the others. Preliminary phytochemical screening revealed the presence of flavonoids, sterols, cucurbitacin saponins, polyphenolics, proteins, and carbohydrates. The results obtained suggest marked antihyperlipidemic and hypolipidemic activity of the extracts.
Jalal Ud Din - One of the best experts on this subject based on the ideXlab platform.
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production of nitrogen fixing azotobacter sr 4 and phosphorus solubilizing aspergillus niger and their evaluation on Lagenaria siceraria and abelmoschus esculentus
Biotechnology Reports, 2019Co-Authors: Misbahud Din, Rubina Nelofer, Muhammad Salman, Faisal Hayat Khan, Asad Khan, Munib Ahmad, Fazal Jalil, Jalal Ud Din, Mudassir KhanAbstract:Abstract Aims Current study was aimed to produce nitrogen fixing Azotobacter sp. (SR-4) and phosphorus solubilizers Aspergillus niger (A. niger) and to evaluate their efficiency as biofertilizers for agricultural practices. Methods Two biofertilizer including nitrogen fixing and phosphorus solubilizing were grown. The nitrogen fixing efficiency of Azotobacter (SR-4) was determined by Kjeldahl method. Similarly, Vanadomoybdate method was used to measure the soluble phosphorus while Heinonen method was used to analyze concentration of phytase and phosphatase in the cultures. Furthermore, both biofertilizers were tested in a field trail on Lagenaria siceraria (bottle gourd) and Abelmoschus esculentus (okra). Results The Azotobacter (SR-4) strain was found efficient nitrogen fixer as 35.08 mg of nitrogen per gram of carbon was produced after 72 h of fermentation. Similarly, A. niger strain excrete extracellular phosphate solubilizing enzymes such as phytase (133UI in 48 h of fermentation) and phosphatase (170UI in 48 h of fermentation) which can solubilize the rock phosphate and make it available to plants. In field trials on selected plants (L. siceraria and A. esculentus), both biofertilizers showed significant increase in plant height, leaf length/width, fruit size and number of fruits per plant when compared with controls/untreated plants. Furthermore, plants co-inoculated with both the N fixing Azotobacter and phosphorus solubilizing A. niger have enhanced performance than those treated with each biofertilizer alone. Conclusion The inoculation of seeds with A. niger and Azotobacter may replace costly and environment toxic chemical fertilizers with environment friendly and cost effective biofertilizers.
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Production of nitrogen fixing Azotobacter (SR-4) and phosphorus solubilizing Aspergillus niger and their evaluation on Lagenaria siceraria and Abelmoschus esculentus
'Elsevier BV', 2019Co-Authors: Misbahud Din, Rubina Nelofer, Muhammad Salman, Faisal Hayat Khan, Asad Khan, Munib Ahmad, Fazal Jalil, Jalal Ud Din, Mudassir KhanAbstract:Aims: Current study was aimed to produce nitrogen fixing Azotobacter sp. (SR-4) and phosphorus solubilizers Aspergillus niger (A. niger) and to evaluate their efficiency as biofertilizers for agricultural practices. Methods: Two biofertilizer including nitrogen fixing and phosphorus solubilizing were grown. The nitrogen fixing efficiency of Azotobacter (SR-4) was determined by Kjeldahl method. Similarly, Vanadomoybdate method was used to measure the soluble phosphorus while Heinonen method was used to analyze concentration of phytase and phosphatase in the cultures. Furthermore, both biofertilizers were tested in a field trail on Lagenaria siceraria (bottle gourd) and Abelmoschus esculentus (okra). Results: The Azotobacter (SR-4) strain was found efficient nitrogen fixer as 35.08 mg of nitrogen per gram of carbon was produced after 72 h of fermentation. Similarly, A. niger strain excrete extracellular phosphate solubilizing enzymes such as phytase (133UI in 48 h of fermentation) and phosphatase (170UI in 48 h of fermentation) which can solubilize the rock phosphate and make it available to plants. In field trials on selected plants (L. siceraria and A. esculentus), both biofertilizers showed significant increase in plant height, leaf length/width, fruit size and number of fruits per plant when compared with controls/untreated plants. Furthermore, plants co-inoculated with both the N fixing Azotobacter and phosphorus solubilizing A. niger have enhanced performance than those treated with each biofertilizer alone. Conclusion: The inoculation of seeds with A. niger and Azotobacter may replace costly and environment toxic chemical fertilizers with environment friendly and cost effective biofertilizers. Keywords: Biofertilizers, Azotobacter, A. niger, Bottle gourd and okr
Fazal Jalil - One of the best experts on this subject based on the ideXlab platform.
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production of nitrogen fixing azotobacter sr 4 and phosphorus solubilizing aspergillus niger and their evaluation on Lagenaria siceraria and abelmoschus esculentus
Biotechnology Reports, 2019Co-Authors: Misbahud Din, Rubina Nelofer, Muhammad Salman, Faisal Hayat Khan, Asad Khan, Munib Ahmad, Fazal Jalil, Jalal Ud Din, Mudassir KhanAbstract:Abstract Aims Current study was aimed to produce nitrogen fixing Azotobacter sp. (SR-4) and phosphorus solubilizers Aspergillus niger (A. niger) and to evaluate their efficiency as biofertilizers for agricultural practices. Methods Two biofertilizer including nitrogen fixing and phosphorus solubilizing were grown. The nitrogen fixing efficiency of Azotobacter (SR-4) was determined by Kjeldahl method. Similarly, Vanadomoybdate method was used to measure the soluble phosphorus while Heinonen method was used to analyze concentration of phytase and phosphatase in the cultures. Furthermore, both biofertilizers were tested in a field trail on Lagenaria siceraria (bottle gourd) and Abelmoschus esculentus (okra). Results The Azotobacter (SR-4) strain was found efficient nitrogen fixer as 35.08 mg of nitrogen per gram of carbon was produced after 72 h of fermentation. Similarly, A. niger strain excrete extracellular phosphate solubilizing enzymes such as phytase (133UI in 48 h of fermentation) and phosphatase (170UI in 48 h of fermentation) which can solubilize the rock phosphate and make it available to plants. In field trials on selected plants (L. siceraria and A. esculentus), both biofertilizers showed significant increase in plant height, leaf length/width, fruit size and number of fruits per plant when compared with controls/untreated plants. Furthermore, plants co-inoculated with both the N fixing Azotobacter and phosphorus solubilizing A. niger have enhanced performance than those treated with each biofertilizer alone. Conclusion The inoculation of seeds with A. niger and Azotobacter may replace costly and environment toxic chemical fertilizers with environment friendly and cost effective biofertilizers.
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Production of nitrogen fixing Azotobacter (SR-4) and phosphorus solubilizing Aspergillus niger and their evaluation on Lagenaria siceraria and Abelmoschus esculentus
'Elsevier BV', 2019Co-Authors: Misbahud Din, Rubina Nelofer, Muhammad Salman, Faisal Hayat Khan, Asad Khan, Munib Ahmad, Fazal Jalil, Jalal Ud Din, Mudassir KhanAbstract:Aims: Current study was aimed to produce nitrogen fixing Azotobacter sp. (SR-4) and phosphorus solubilizers Aspergillus niger (A. niger) and to evaluate their efficiency as biofertilizers for agricultural practices. Methods: Two biofertilizer including nitrogen fixing and phosphorus solubilizing were grown. The nitrogen fixing efficiency of Azotobacter (SR-4) was determined by Kjeldahl method. Similarly, Vanadomoybdate method was used to measure the soluble phosphorus while Heinonen method was used to analyze concentration of phytase and phosphatase in the cultures. Furthermore, both biofertilizers were tested in a field trail on Lagenaria siceraria (bottle gourd) and Abelmoschus esculentus (okra). Results: The Azotobacter (SR-4) strain was found efficient nitrogen fixer as 35.08 mg of nitrogen per gram of carbon was produced after 72 h of fermentation. Similarly, A. niger strain excrete extracellular phosphate solubilizing enzymes such as phytase (133UI in 48 h of fermentation) and phosphatase (170UI in 48 h of fermentation) which can solubilize the rock phosphate and make it available to plants. In field trials on selected plants (L. siceraria and A. esculentus), both biofertilizers showed significant increase in plant height, leaf length/width, fruit size and number of fruits per plant when compared with controls/untreated plants. Furthermore, plants co-inoculated with both the N fixing Azotobacter and phosphorus solubilizing A. niger have enhanced performance than those treated with each biofertilizer alone. Conclusion: The inoculation of seeds with A. niger and Azotobacter may replace costly and environment toxic chemical fertilizers with environment friendly and cost effective biofertilizers. Keywords: Biofertilizers, Azotobacter, A. niger, Bottle gourd and okr
