Lamiaceae

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Hugh D. Wilson - One of the best experts on this subject based on the ideXlab platform.

Sanda Vladimir-knežević - One of the best experts on this subject based on the ideXlab platform.

  • Total Hydroxycinnamic Acids Assay: Prevalidation and Application on Lamiaceae Species
    Food Analytical Methods, 2014
    Co-Authors: Maja Bival Štefan, Jadranka Vuković Rodríguez, Biljana Blažeković, Marija Kindl, Sanda Vladimir-knežević
    Abstract:

    In this work, qualitative and quantitative analyses of total hydroxycinnamic acid (THA) derivatives in leaves, flowers and aerial parts of 32 Lamiaceae species were carried out. HPTLC analysis of plant material indicated rosmarinic acid as the main phenolic acid in 22 Lamiaceae plant extracts, while chlorogenic acid predominated in other 10 investigated species. Determination of THA in different medicinal plants was performed by colorimetric methods using chromogenic system of HCl-NaNO_2–Na_2MoO_4–NaOH and two standards: rosmarinic acid (R-THA procedure) and chlorogenic acid (C-THA procedure). Performance characteristics of THA assay were established, and the quality of the analytical procedures was evaluated using valuable prevalidation strategy. Good quality of the measurements at the lower analyte level, excellent resolution of blank and analyte signals, homogenous data material, ideal linear calibration and analytical evaluation functions, very low limit of detection [ L _D(R-THA) = 0.53 μg; L _D(C-THD) = 1.75 μg] and limit of quantitation [ L _Q(R-THD) = 1.76 μg; L _Q(C-THD) = 4.21 μg], and high precision and accuracy confirmed the high quality of investigated procedures as valuable tools in THA analysis. Application of these prevalidated, simple and rapid analytical methods revealed that the studied Lamiaceae plants differ greatly in their THA contents, ranging from 1.95 % ( Acinos arvensis ) to 11.03 % ( Satureja subspicata ). The results obtained from qualitative and quantitative analysis provided new information regarding phytochemical characterization of Lamiaceae species originating from Croatia and highlighted members of the genus Micromeria , Origanum , Melissa and Satureja as rich sources of hydroxycinnamic acids.

Marija Kindl - One of the best experts on this subject based on the ideXlab platform.

  • Total Hydroxycinnamic Acids Assay: Prevalidation and Application on Lamiaceae Species
    Food Analytical Methods, 2014
    Co-Authors: Maja Bival Štefan, Jadranka Vuković Rodríguez, Biljana Blažeković, Marija Kindl, Sanda Vladimir-knežević
    Abstract:

    In this work, qualitative and quantitative analyses of total hydroxycinnamic acid (THA) derivatives in leaves, flowers and aerial parts of 32 Lamiaceae species were carried out. HPTLC analysis of plant material indicated rosmarinic acid as the main phenolic acid in 22 Lamiaceae plant extracts, while chlorogenic acid predominated in other 10 investigated species. Determination of THA in different medicinal plants was performed by colorimetric methods using chromogenic system of HCl-NaNO_2–Na_2MoO_4–NaOH and two standards: rosmarinic acid (R-THA procedure) and chlorogenic acid (C-THA procedure). Performance characteristics of THA assay were established, and the quality of the analytical procedures was evaluated using valuable prevalidation strategy. Good quality of the measurements at the lower analyte level, excellent resolution of blank and analyte signals, homogenous data material, ideal linear calibration and analytical evaluation functions, very low limit of detection [ L _D(R-THA) = 0.53 μg; L _D(C-THD) = 1.75 μg] and limit of quantitation [ L _Q(R-THD) = 1.76 μg; L _Q(C-THD) = 4.21 μg], and high precision and accuracy confirmed the high quality of investigated procedures as valuable tools in THA analysis. Application of these prevalidated, simple and rapid analytical methods revealed that the studied Lamiaceae plants differ greatly in their THA contents, ranging from 1.95 % ( Acinos arvensis ) to 11.03 % ( Satureja subspicata ). The results obtained from qualitative and quantitative analysis provided new information regarding phytochemical characterization of Lamiaceae species originating from Croatia and highlighted members of the genus Micromeria , Origanum , Melissa and Satureja as rich sources of hydroxycinnamic acids.

  • acetylcholinesterase inhibitory antioxidant and phytochemical properties of selected medicinal plants of the Lamiaceae family
    Molecules, 2014
    Co-Authors: Sanda Vladimirkneževic, Marija Kindl, Biljana Blažekovic, Jelena Vladic, Agnieszka D Lowernedza, Adelheid Brantner
    Abstract:

    The present study aimed to evaluate acetylcholinesterase (AChE) inhibitory and antioxidant activities of Lamiaceae medicinal plants growing wild in Croatia. Using Ellman’s colorimetric assay all tested ethanolic extracts and their hydroxycinnamic acid constituents demonstrated in vitro AChE inhibitory properties in a dose dependent manner. The extracts of Mentha x piperita, M. longifolia, Salvia officinalis, Satureja montana, Teucrium arduini, T. chamaedrys, T. montanum, T. polium and Thymus vulgaris at 1 mg/mL showed strong inhibitory activity against AChE. The antioxidant potential of the investigated Lamiaceae species was assessed by DPPH• scavenging activity and total antioxidant capacity assays, in comparison with hydroxycinnamic acids and trolox. The extracts differed greatly in their total hydroxycinnamic derivatives content, determined spectrophotometrically. Rosmarinic acid was found to be the predominant constituent in most of the investigated medicinal plants (by RP-HPLC) and had a substantial influence on their AChE inhibitory and antioxidant properties, with the exception of Teucrium species. These findings indicate that Lamiaceae species are a rich source of various natural AChE inhibitors and antioxidants that could be useful in the prevention and treatment of Alzheimer’s and other related diseases.

Hugh D. Wilson - One of the best experts on this subject based on the ideXlab platform.

Maja Bival Štefan - One of the best experts on this subject based on the ideXlab platform.

  • Total Hydroxycinnamic Acids Assay: Prevalidation and Application on Lamiaceae Species
    Food Analytical Methods, 2014
    Co-Authors: Maja Bival Štefan, Jadranka Vuković Rodríguez, Biljana Blažeković, Marija Kindl, Sanda Vladimir-knežević
    Abstract:

    In this work, qualitative and quantitative analyses of total hydroxycinnamic acid (THA) derivatives in leaves, flowers and aerial parts of 32 Lamiaceae species were carried out. HPTLC analysis of plant material indicated rosmarinic acid as the main phenolic acid in 22 Lamiaceae plant extracts, while chlorogenic acid predominated in other 10 investigated species. Determination of THA in different medicinal plants was performed by colorimetric methods using chromogenic system of HCl-NaNO_2–Na_2MoO_4–NaOH and two standards: rosmarinic acid (R-THA procedure) and chlorogenic acid (C-THA procedure). Performance characteristics of THA assay were established, and the quality of the analytical procedures was evaluated using valuable prevalidation strategy. Good quality of the measurements at the lower analyte level, excellent resolution of blank and analyte signals, homogenous data material, ideal linear calibration and analytical evaluation functions, very low limit of detection [ L _D(R-THA) = 0.53 μg; L _D(C-THD) = 1.75 μg] and limit of quantitation [ L _Q(R-THD) = 1.76 μg; L _Q(C-THD) = 4.21 μg], and high precision and accuracy confirmed the high quality of investigated procedures as valuable tools in THA analysis. Application of these prevalidated, simple and rapid analytical methods revealed that the studied Lamiaceae plants differ greatly in their THA contents, ranging from 1.95 % ( Acinos arvensis ) to 11.03 % ( Satureja subspicata ). The results obtained from qualitative and quantitative analysis provided new information regarding phytochemical characterization of Lamiaceae species originating from Croatia and highlighted members of the genus Micromeria , Origanum , Melissa and Satureja as rich sources of hydroxycinnamic acids.