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Vijaya Saradhi V Upadhyayula - One of the best experts on this subject based on the ideXlab platform.
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acetylcholinesterase inhibitory activity of stigmasterol hexacosanol is responsible for Larvicidal and repellent properties of chromolaena odorata
Biochimica et Biophysica Acta, 2017Co-Authors: Sriramya Gade, Maheshwari Rajamanikyam, Varahalarao Vadlapudi, Krishna Madhav Nukala, Rajendar Aluvala, Chandrasekhar Giddigari, Naga Jyothi Karanam, Richa Pandey, Nabin C Barua, Vijaya Saradhi V UpadhyayulaAbstract:Abstract Background Chromolaena odorata , has been traditionally known for its insect repellent property. Aim of this study was to determine Larvicidal tendency of C. odorata on Culex quinquefasciatus and isolate compounds responsible for this activity and to determine the mechanism of action of these compounds. Methods C. odorata plant extract was screened for mosquito Larvicidal activity. The extract was fractionated using chromatography and the bioactive fraction showing Larvicidal activity was identified. The chemical nature of the compounds in the bioactive fraction was determined using NMR and Mass spectrometry. Results We identified phytosterols and alkanols to be the compounds regulating Larvicidal activity in the bioactive fraction of the plant extract. Stigmasterol and 1-hexacosanol were identified to be the chief orchestrators of Larvicidal activity and their mode of action has been observed to be neurotoxicity. At a molecular level both stigmasterol and 1-hexacosanol were found to be inhibiting acetylcholinesterase activity in C. quinquefasciatus & A. aegypti. The acetylcholinesterase inhibitory effect was validated in vitro using recombinant acetylcholinesterase and ex vivo in larval homogenates of Culex and Aedes . Electrophysiological studies using electroantennography have shown enhanced neural response to these compounds. Conclusions Neurotoxic effect of C. odorata derived stigmasterol and 1-hexacosanol, exerted through acetylcholinesterase inhibition was responsible for the mortality of C. quinquefasciatus , A. aegypti & Chironomus riparius . EAG studies pointed out hyper-excitability of the olfactory system by these compounds. General significance These compounds are natural agents for mosquito control that can be used in vector control as Larvicidal compounds, pending further investigations.
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Acetylcholinesterase inhibitory activity of stigmasterol & hexacosanol is responsible for Larvicidal and repellent properties of Chromolaena odorata
Biochimica et Biophysica Acta, 2016Co-Authors: Sriramya Gade, Maheshwari Rajamanikyam, Varahalarao Vadlapudi, Krishna Madhav Nukala, Rajendar Aluvala, Chandrasekhar Giddigari, Naga Jyothi Karanam, Richa Pandey, Nabin C Barua, Vijaya Saradhi V UpadhyayulaAbstract:Abstract Background Chromolaena odorata , has been traditionally known for its insect repellent property. Aim of this study was to determine Larvicidal tendency of C. odorata on Culex quinquefasciatus and isolate compounds responsible for this activity and to determine the mechanism of action of these compounds. Methods C. odorata plant extract was screened for mosquito Larvicidal activity. The extract was fractionated using chromatography and the bioactive fraction showing Larvicidal activity was identified. The chemical nature of the compounds in the bioactive fraction was determined using NMR and Mass spectrometry. Results We identified phytosterols and alkanols to be the compounds regulating Larvicidal activity in the bioactive fraction of the plant extract. Stigmasterol and 1-hexacosanol were identified to be the chief orchestrators of Larvicidal activity and their mode of action has been observed to be neurotoxicity. At a molecular level both stigmasterol and 1-hexacosanol were found to be inhibiting acetylcholinesterase activity in C. quinquefasciatus & A. aegypti. The acetylcholinesterase inhibitory effect was validated in vitro using recombinant acetylcholinesterase and ex vivo in larval homogenates of Culex and Aedes . Electrophysiological studies using electroantennography have shown enhanced neural response to these compounds. Conclusions Neurotoxic effect of C. odorata derived stigmasterol and 1-hexacosanol, exerted through acetylcholinesterase inhibition was responsible for the mortality of C. quinquefasciatus , A. aegypti & Chironomus riparius . EAG studies pointed out hyper-excitability of the olfactory system by these compounds. General significance These compounds are natural agents for mosquito control that can be used in vector control as Larvicidal compounds, pending further investigations.
Sriramya Gade - One of the best experts on this subject based on the ideXlab platform.
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acetylcholinesterase inhibitory activity of stigmasterol hexacosanol is responsible for Larvicidal and repellent properties of chromolaena odorata
Biochimica et Biophysica Acta, 2017Co-Authors: Sriramya Gade, Maheshwari Rajamanikyam, Varahalarao Vadlapudi, Krishna Madhav Nukala, Rajendar Aluvala, Chandrasekhar Giddigari, Naga Jyothi Karanam, Richa Pandey, Nabin C Barua, Vijaya Saradhi V UpadhyayulaAbstract:Abstract Background Chromolaena odorata , has been traditionally known for its insect repellent property. Aim of this study was to determine Larvicidal tendency of C. odorata on Culex quinquefasciatus and isolate compounds responsible for this activity and to determine the mechanism of action of these compounds. Methods C. odorata plant extract was screened for mosquito Larvicidal activity. The extract was fractionated using chromatography and the bioactive fraction showing Larvicidal activity was identified. The chemical nature of the compounds in the bioactive fraction was determined using NMR and Mass spectrometry. Results We identified phytosterols and alkanols to be the compounds regulating Larvicidal activity in the bioactive fraction of the plant extract. Stigmasterol and 1-hexacosanol were identified to be the chief orchestrators of Larvicidal activity and their mode of action has been observed to be neurotoxicity. At a molecular level both stigmasterol and 1-hexacosanol were found to be inhibiting acetylcholinesterase activity in C. quinquefasciatus & A. aegypti. The acetylcholinesterase inhibitory effect was validated in vitro using recombinant acetylcholinesterase and ex vivo in larval homogenates of Culex and Aedes . Electrophysiological studies using electroantennography have shown enhanced neural response to these compounds. Conclusions Neurotoxic effect of C. odorata derived stigmasterol and 1-hexacosanol, exerted through acetylcholinesterase inhibition was responsible for the mortality of C. quinquefasciatus , A. aegypti & Chironomus riparius . EAG studies pointed out hyper-excitability of the olfactory system by these compounds. General significance These compounds are natural agents for mosquito control that can be used in vector control as Larvicidal compounds, pending further investigations.
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Acetylcholinesterase inhibitory activity of stigmasterol & hexacosanol is responsible for Larvicidal and repellent properties of Chromolaena odorata
Biochimica et Biophysica Acta, 2016Co-Authors: Sriramya Gade, Maheshwari Rajamanikyam, Varahalarao Vadlapudi, Krishna Madhav Nukala, Rajendar Aluvala, Chandrasekhar Giddigari, Naga Jyothi Karanam, Richa Pandey, Nabin C Barua, Vijaya Saradhi V UpadhyayulaAbstract:Abstract Background Chromolaena odorata , has been traditionally known for its insect repellent property. Aim of this study was to determine Larvicidal tendency of C. odorata on Culex quinquefasciatus and isolate compounds responsible for this activity and to determine the mechanism of action of these compounds. Methods C. odorata plant extract was screened for mosquito Larvicidal activity. The extract was fractionated using chromatography and the bioactive fraction showing Larvicidal activity was identified. The chemical nature of the compounds in the bioactive fraction was determined using NMR and Mass spectrometry. Results We identified phytosterols and alkanols to be the compounds regulating Larvicidal activity in the bioactive fraction of the plant extract. Stigmasterol and 1-hexacosanol were identified to be the chief orchestrators of Larvicidal activity and their mode of action has been observed to be neurotoxicity. At a molecular level both stigmasterol and 1-hexacosanol were found to be inhibiting acetylcholinesterase activity in C. quinquefasciatus & A. aegypti. The acetylcholinesterase inhibitory effect was validated in vitro using recombinant acetylcholinesterase and ex vivo in larval homogenates of Culex and Aedes . Electrophysiological studies using electroantennography have shown enhanced neural response to these compounds. Conclusions Neurotoxic effect of C. odorata derived stigmasterol and 1-hexacosanol, exerted through acetylcholinesterase inhibition was responsible for the mortality of C. quinquefasciatus , A. aegypti & Chironomus riparius . EAG studies pointed out hyper-excitability of the olfactory system by these compounds. General significance These compounds are natural agents for mosquito control that can be used in vector control as Larvicidal compounds, pending further investigations.
V Venkatesalu - One of the best experts on this subject based on the ideXlab platform.
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Larvicidal Activity of Neolamarckia cadamba Against the Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus
Proceedings of the Zoological Society, 2020Co-Authors: Sofi Imtiyaz Ali, V VenkatesaluAbstract:The excessive use of conventional insecticides to Culicidae leads to the emergence of mosquitoe resistant, adverse environmental and human effects. Therefore, the present study was carried out to explore the Larvicidal efficacy of petroleum ether, chloroform, ethyl acetate and methanol extracts of roots, leaves, and bark of Neolamarckia cadamba against the early 4th instar larvae of Anopheles stephensi , Aedes aegypti and Culex quinquefasciatus at concentrations ranging from 12.5 to 200 ppm under laboratory conditions. The root extracts showed the highest Larvicidal potential than the leaf and bark extracts. After 24 h of exposure period, the highest Larvicidal activities were observed in the methanol extract of roots with LC_50 value of 43.29 and LC_90 value of 202.85 ppm against An. stephensi ; LC_50 value of 70.82 and LC_90 value of 253.73 ppm against Ae. Aegypti and LC_50 value of 99.51 and LC_90 value of 311.02 ppm against the Cx. Quinquefasciatus , respectively. Thus, the plant extracts of N. cadamba showed considerable mosquito Larvicidal activities and can be considered for further investigation.
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differential Larvicidal efficacy of four species of vitex against culex quinquefasciatus larvae
Parasitology Research, 2007Co-Authors: Krishnan Kannathasan, A Senthilkumar, M Chandrasekaran, V VenkatesaluAbstract:The early fourth instar larvae of Culex quinquefasciatus, reared in the laboratory were used for Larvicidal assay with leaf extracts of Vitex negundo, Vitex trifolia, Vitex peduncularis and Vitex altissima. The methanol extracts of the four species possessed varying levels of Larvicidal nature. The highest Larvicidal activity was found with the extract of V. trifolia (LC50 = 41.41 ppm) followed by V. peduncularis (LC50 = 76.28 ppm), V. altissima (LC50 = 128.04 ppm) and V. negundo (LC50 = 212.57 ppm).
Krishna Madhav Nukala - One of the best experts on this subject based on the ideXlab platform.
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acetylcholinesterase inhibitory activity of stigmasterol hexacosanol is responsible for Larvicidal and repellent properties of chromolaena odorata
Biochimica et Biophysica Acta, 2017Co-Authors: Sriramya Gade, Maheshwari Rajamanikyam, Varahalarao Vadlapudi, Krishna Madhav Nukala, Rajendar Aluvala, Chandrasekhar Giddigari, Naga Jyothi Karanam, Richa Pandey, Nabin C Barua, Vijaya Saradhi V UpadhyayulaAbstract:Abstract Background Chromolaena odorata , has been traditionally known for its insect repellent property. Aim of this study was to determine Larvicidal tendency of C. odorata on Culex quinquefasciatus and isolate compounds responsible for this activity and to determine the mechanism of action of these compounds. Methods C. odorata plant extract was screened for mosquito Larvicidal activity. The extract was fractionated using chromatography and the bioactive fraction showing Larvicidal activity was identified. The chemical nature of the compounds in the bioactive fraction was determined using NMR and Mass spectrometry. Results We identified phytosterols and alkanols to be the compounds regulating Larvicidal activity in the bioactive fraction of the plant extract. Stigmasterol and 1-hexacosanol were identified to be the chief orchestrators of Larvicidal activity and their mode of action has been observed to be neurotoxicity. At a molecular level both stigmasterol and 1-hexacosanol were found to be inhibiting acetylcholinesterase activity in C. quinquefasciatus & A. aegypti. The acetylcholinesterase inhibitory effect was validated in vitro using recombinant acetylcholinesterase and ex vivo in larval homogenates of Culex and Aedes . Electrophysiological studies using electroantennography have shown enhanced neural response to these compounds. Conclusions Neurotoxic effect of C. odorata derived stigmasterol and 1-hexacosanol, exerted through acetylcholinesterase inhibition was responsible for the mortality of C. quinquefasciatus , A. aegypti & Chironomus riparius . EAG studies pointed out hyper-excitability of the olfactory system by these compounds. General significance These compounds are natural agents for mosquito control that can be used in vector control as Larvicidal compounds, pending further investigations.
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Acetylcholinesterase inhibitory activity of stigmasterol & hexacosanol is responsible for Larvicidal and repellent properties of Chromolaena odorata
Biochimica et Biophysica Acta, 2016Co-Authors: Sriramya Gade, Maheshwari Rajamanikyam, Varahalarao Vadlapudi, Krishna Madhav Nukala, Rajendar Aluvala, Chandrasekhar Giddigari, Naga Jyothi Karanam, Richa Pandey, Nabin C Barua, Vijaya Saradhi V UpadhyayulaAbstract:Abstract Background Chromolaena odorata , has been traditionally known for its insect repellent property. Aim of this study was to determine Larvicidal tendency of C. odorata on Culex quinquefasciatus and isolate compounds responsible for this activity and to determine the mechanism of action of these compounds. Methods C. odorata plant extract was screened for mosquito Larvicidal activity. The extract was fractionated using chromatography and the bioactive fraction showing Larvicidal activity was identified. The chemical nature of the compounds in the bioactive fraction was determined using NMR and Mass spectrometry. Results We identified phytosterols and alkanols to be the compounds regulating Larvicidal activity in the bioactive fraction of the plant extract. Stigmasterol and 1-hexacosanol were identified to be the chief orchestrators of Larvicidal activity and their mode of action has been observed to be neurotoxicity. At a molecular level both stigmasterol and 1-hexacosanol were found to be inhibiting acetylcholinesterase activity in C. quinquefasciatus & A. aegypti. The acetylcholinesterase inhibitory effect was validated in vitro using recombinant acetylcholinesterase and ex vivo in larval homogenates of Culex and Aedes . Electrophysiological studies using electroantennography have shown enhanced neural response to these compounds. Conclusions Neurotoxic effect of C. odorata derived stigmasterol and 1-hexacosanol, exerted through acetylcholinesterase inhibition was responsible for the mortality of C. quinquefasciatus , A. aegypti & Chironomus riparius . EAG studies pointed out hyper-excitability of the olfactory system by these compounds. General significance These compounds are natural agents for mosquito control that can be used in vector control as Larvicidal compounds, pending further investigations.
Rajendar Aluvala - One of the best experts on this subject based on the ideXlab platform.
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acetylcholinesterase inhibitory activity of stigmasterol hexacosanol is responsible for Larvicidal and repellent properties of chromolaena odorata
Biochimica et Biophysica Acta, 2017Co-Authors: Sriramya Gade, Maheshwari Rajamanikyam, Varahalarao Vadlapudi, Krishna Madhav Nukala, Rajendar Aluvala, Chandrasekhar Giddigari, Naga Jyothi Karanam, Richa Pandey, Nabin C Barua, Vijaya Saradhi V UpadhyayulaAbstract:Abstract Background Chromolaena odorata , has been traditionally known for its insect repellent property. Aim of this study was to determine Larvicidal tendency of C. odorata on Culex quinquefasciatus and isolate compounds responsible for this activity and to determine the mechanism of action of these compounds. Methods C. odorata plant extract was screened for mosquito Larvicidal activity. The extract was fractionated using chromatography and the bioactive fraction showing Larvicidal activity was identified. The chemical nature of the compounds in the bioactive fraction was determined using NMR and Mass spectrometry. Results We identified phytosterols and alkanols to be the compounds regulating Larvicidal activity in the bioactive fraction of the plant extract. Stigmasterol and 1-hexacosanol were identified to be the chief orchestrators of Larvicidal activity and their mode of action has been observed to be neurotoxicity. At a molecular level both stigmasterol and 1-hexacosanol were found to be inhibiting acetylcholinesterase activity in C. quinquefasciatus & A. aegypti. The acetylcholinesterase inhibitory effect was validated in vitro using recombinant acetylcholinesterase and ex vivo in larval homogenates of Culex and Aedes . Electrophysiological studies using electroantennography have shown enhanced neural response to these compounds. Conclusions Neurotoxic effect of C. odorata derived stigmasterol and 1-hexacosanol, exerted through acetylcholinesterase inhibition was responsible for the mortality of C. quinquefasciatus , A. aegypti & Chironomus riparius . EAG studies pointed out hyper-excitability of the olfactory system by these compounds. General significance These compounds are natural agents for mosquito control that can be used in vector control as Larvicidal compounds, pending further investigations.
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Acetylcholinesterase inhibitory activity of stigmasterol & hexacosanol is responsible for Larvicidal and repellent properties of Chromolaena odorata
Biochimica et Biophysica Acta, 2016Co-Authors: Sriramya Gade, Maheshwari Rajamanikyam, Varahalarao Vadlapudi, Krishna Madhav Nukala, Rajendar Aluvala, Chandrasekhar Giddigari, Naga Jyothi Karanam, Richa Pandey, Nabin C Barua, Vijaya Saradhi V UpadhyayulaAbstract:Abstract Background Chromolaena odorata , has been traditionally known for its insect repellent property. Aim of this study was to determine Larvicidal tendency of C. odorata on Culex quinquefasciatus and isolate compounds responsible for this activity and to determine the mechanism of action of these compounds. Methods C. odorata plant extract was screened for mosquito Larvicidal activity. The extract was fractionated using chromatography and the bioactive fraction showing Larvicidal activity was identified. The chemical nature of the compounds in the bioactive fraction was determined using NMR and Mass spectrometry. Results We identified phytosterols and alkanols to be the compounds regulating Larvicidal activity in the bioactive fraction of the plant extract. Stigmasterol and 1-hexacosanol were identified to be the chief orchestrators of Larvicidal activity and their mode of action has been observed to be neurotoxicity. At a molecular level both stigmasterol and 1-hexacosanol were found to be inhibiting acetylcholinesterase activity in C. quinquefasciatus & A. aegypti. The acetylcholinesterase inhibitory effect was validated in vitro using recombinant acetylcholinesterase and ex vivo in larval homogenates of Culex and Aedes . Electrophysiological studies using electroantennography have shown enhanced neural response to these compounds. Conclusions Neurotoxic effect of C. odorata derived stigmasterol and 1-hexacosanol, exerted through acetylcholinesterase inhibition was responsible for the mortality of C. quinquefasciatus , A. aegypti & Chironomus riparius . EAG studies pointed out hyper-excitability of the olfactory system by these compounds. General significance These compounds are natural agents for mosquito control that can be used in vector control as Larvicidal compounds, pending further investigations.
